1 results about "Candidate gene" patented technology

The invention relates to a screening method of silkworm hemocyte specific expressed genes and a method for analyzing and finding a hemocyte specific expression regulation element through cloning of a protease O gene promoter. The hemocyte specific expression regulation element is manufactured through the following steps that 1, a hemocyte specific expression candidate gene protease O gene is screened out through silkworm tissue chip expression data, transcriptome data, a quantitive RT-PCR and other methods, and the silkworm hemocyte specific expression of the gene is verified successfully; 2, a related gene promoter is successfully cloned, and functional analysis is carried out; 3, a Bac-to-Bac rhabdovirus expression system is successfully improved, an effective hemocyte promoter detection system is built, the activity of the candidate gene promoter is detected at the cell and the individual level, and tissue specificity of the promoter is verified; 4, the silkworm hemocyte specific gene protease O gene promoter is obtained through the study, and it is analyzed and found that the promoter is provided with the tissue specificity regulation element in the section between the upstream -333 and -80 of a transcriptional start site. The specificity expression of the gene on hemocyte is controlled.