The invention discloses a method for detecting HSL gene expression in bovine muscle. Through designing HSL gene specific primer pairs, amplification of internal primers of GAPDH qPCR, and the relativequantitative analysis to the result of the qPCR, the method for detecting HSL gene expression in the bovine muscle accurately and rapidly analysis the HSL gene expression in the bovine muscle. The established qPCR method for detecting HSL gene expression level can be used to accurately detect the HSL gene expression level of different cattle muscle. The method can be used to detect the expressionlevel of the bovine muscle HSL gene in different breeds, providing a reference for in-depth study of bovine HSL gene function. The method is easy and simple to operate.