The invention belongs to the technical field of marine organisms, and concretely relates to a method for the efficient ultralow temperature cryopreservation of turbot sperms. The method comprises the following steps: mixing the seminal fluid of turbots with an antifreeze liquid according to a volume ratio of 3:1-4:1, carrying out two-step cooling treatment, carrying out one-step balancing, adding to liquid nitrogen (having a temperature of -196DEG C), and preserving; and the antifreeze liquid is composed of a diluent, an antifreeze agent and an additive. High-quality frozen sperms obtained through the method for the efficient ultralow temperature cryopreservation of turbot sperms are of great significance of the artificial breeding, germplasm preservation, heredity diversity and sustainable culturing of the turbots.