The invention discloses a tyrosine decarboxylase mutant and a gene and application thereof. The mutant is obtained after serine at the site 586 of tyrosine decarboxylase which comes from lactobacillus brevis and has an amino acid sequence shown as SEQ ID No:1 in a sequence table is substituted by alanine. The specific activity of the tyrosine decarboxylase mutant on the substrate tyrosine is 2.23 times that of wild-type tyrosine decarboxylase, and the Km value on the substrate is 72.4% that of the wild-type tyrosine decarboxylase. The tyrosine decarboxylase mutant is more suitable for preparation of tyramine products than the wild-type tyrosine decarboxylase and has a good industrial development prospect.