Molecule technical method for identifying indica/japonica type of Yunnan rice old variety of Yunnan rice old variety

[0021] Thirty rice seed resources from high altitude in Yunnan were identified by "morphological index method" and molecular identification method to identify the indica and japonica characteristics of the materials, and the identification results of the two methods were compared to investigate the reliability of the molecular identification method.

[0022] For molecular identification, 5 seeds of the material to be identified were germinated at 20°C, 5 days after germination, the leaves were taken to extract the total DNA of rice, the DNA concentration of the sample was measured and the DNA was quantitatively diluted to 25ng / ul, and then PCR reaction was carried out with the specific primer JI; 2% agarose (containing EtBr0.1ug / ml) gel electrophoresis separation; PCR amplification products and electrophoresis results were detected on an ultraviolet transilluminator, and the indica and japonica characteristics of the material were identified according to the electrophoresis res

[0033] The indica and japonica characteristics of the materials were identified by "morphological index method" and molecular identification method respectively, and the identification results of the two methods were compared to investigate the reliability of the molecular identification method.

[0034] For molecular identification, 5 seeds of the material to be identified were germinated at 35°C, 5 days after germination, the leaves were taken to extract the total DNA of rice, the DNA concentration of the sample was measured and the DNA was quantitatively diluted to 25ng / ul, and then PCR reaction was performed with specific primer JI; 2% agarose (containing EtBr0.1ug / ml) gel electrophoresis separation; PCR amplification products and electrophoresis results were detected on an ultraviolet transilluminator, and the indica and japonica characteristics of the material were identified according to the electrophoresis results. There are two products with molecular weights of 280bp

[0044] 120 accessions of rice seed resources from low altitude in Yunnan were identified by "morphological index method" and molecular identification method to identify the indica and japonica characteristics of the materials, and the identification results of the two methods were compared to investigate the reliability of the molecular identification method.

[0045] For molecular identification, 5 seeds of the material to be identified were germinated at 30°C, 5 days after germination, the leaves were taken to extract the total DNA of rice, the DNA concentration of the sample was measured and the DNA was quantitatively diluted to 25ng / ul, and then PCR reaction was performed with specific primer JI; 2% agarose (containing EtBr0.1ug / ml) gel electrophoresis separation; PCR amplification products and electrophoresis results were detected on an ultraviolet transilluminator, and the indica and japonica characteristics of the material were identified according to the electrophoresis