336 results about "Biology" patented technology

A method of modifying the content of certain chemical compounds in tobacco materials is provided, the method including treatment of a tobacco plant component with at least one probiotic. For example, the method may modify the asparagine content in tobacco materials, which can result in a modification in acrylamide production when the tobacco material is exposed to elevated temperatures. The type of tobacco plant component treated according to the invention can be a tobacco seed, a tobacco seedling, an immature live plant, a mature live plant, a harvested plant, or a portion thereof. Examples of probiotics include probiotic species of the genera bifidobacterium, lactobacillus, enterococcus, proionobacterium, bacillus, saccharomyces, streptococcus, and mixtures thereof. Smoking articles and other tobacco products including such probiotic-treated tobacco materials are also provided.

Methods and compositions for producing siRNAs, e.g., in the form of a d-siRNA composition, from dsRNAs are provided. In the subject methods, a dsRNA is contacted with a composition that includes an activity that cleaves dsRNA into siRNAs, where the composition efficiently cleaves dsRNA into siRNAs. siRNAs produced by the subject methods find use in a variety of applications, particularly in applications where the specific reduction or silencing of a gene is desired. Also provided are kits for use in practicing the subject invention.

A catheter system and method is provided for overcoming various problems commonly associated with the application of regional anesthesia. Disclosed herein are several embodiments for providing continuous regional anesthesia. In general, the catheter system includes an epidural catheter, an epidural needle, tubing for optional fluid administration, an attached conductive wire (for connection to a nerve stimulator) and a catheter introducer, which is integrated within the proximal hub of the insulated needle. The embodiments may differ in several ways.

The invention discloses a remediation method for cadmium-contaminated soil, and belongs to the field of soil remediation. In the remediation method, wood vinegar and biochar are prepared from rice straw and/or oilseed rape straw; paddy rice is planted in the cadmium-contaminated soil from May to October of each year firstly, and biochar is applied before paddy rice planting, so that paddy rice conforming to the national standard can be prepared in the cadmium-contaminated soil (to be specific, the cadmium content conforms to the national standard); oilseed rape is planted in the cadmium-contaminated soil from the time after the harvest of the paddy rice to May of next year, and wood vinegar is applied in a specific period to remove cadmium in the cadmium-contaminated soil safely and effectively, so that cadmium in the soil is effectively removed. Moreover, the method effectively utilizes the whole year, thereby guaranteeing the harvest of cereal crops while realizing the remediation of contaminated soil.

The invention discloses a solid microbial agent used for remedying the soil contaminated by petroleum, belonging to the technical field of bioremediation of soil contaminants. First-class seed liquid of acinetobacter radioresistens and rhodococcus erythropolis are mixed in equal parts after two steps of fermentation in liquid and solid states and cultivation to obtain the solid microbial agent, wherein, the number of effective viable bacteria of acinetobacter radioresistens and rhodococcus erythropolis is 10 to 10 CFU/g. The solid microbial agent in the invention has the advantages of low cost, high number of viable bacteria; the invention further has the advantages that micro-ecological environment improvement of the soil can be improved and secondary contamination cannot be caused to the environment, thus applicable to the bioremediation of the soil which is contaminated by petroleum and petroleum products.

Provided are peptidomimetic protein-binding arrays, their manufacture, use, and application. The protein-binding array elements of the invention include a peptidomimetic segment linked to a solid support via a stable anchor. The invention contemplates peptidomimetic array element library synthesis, distribution, and spotting of array elements onto solid planar substrates, labeling of complex protein mixtures, and the analysis of differential protein binding to the array. The invention also enables the enrichment or purification, and subsequent sequencing or structural analysis of proteins that are identified as differential by the array screen. Kits including proteomic microarrays in accordance with the present invention are also provided.

The present invention provides compositions and methods for the detection and characterization of mutations associated with cystic fibrosis. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of mutations in the CFTR gene associated with cystic fibrosis. The present invention also provides compositions, methods and kits for screening sets of CFTR alleles in a single reaction container.

The invention relates to an exosome detecting method based on adapter and rolling ring amplification. An electrochemical sensor method is applied for constructing an adapter probe based on exosome singularity. A signal is generated by means of H2O2 reaction catalysis by G-tetrad-Hemin simulated peroxidase. A large number of G-tetrads are synthesized through rolling ring amplification for performing signal amplification. The method realizes quantitative detection for the exosomes and is used for detecting an actual serum sample. According to the method of the invention, the adapter probe basedon the exosome singularity is constructed through applying an electrochemical sensor method; the signal is generated through H2O2 catalysis by the G-tetrad-Hemin simulated peroxidase; and furthermorea large number of G-tetrads are synthesized through rolling ring amplification for realizing signal amplification, thereby greatly improving detecting sensitivity of the exosome.

The invention discloses a mushroom cultivating material which is characterized in that bamboo sawdust, bamboo shells and biogas residues are used as raw materials, and the water content of the cultivating material is 62-65%. The invention also provides a manufacturing method of the cultivating material. Compared with the prior art, the mushroom cultivating material has the advantages that firstly, the cultivating material is reasonable in proportion, and a fungous material keeps certain air permeability, so that the growth speed of mycelia is increased, and the sackful time is shortened; secondly, bamboo sawdust particles are hard in texture and resistant to decomposition, and the biogas residues contains comprehensive nutrients including nitrogen, phosphorus and potassium, so that the growth of mushroom is promoted, the yield of a product is increased, and the quality of the product is improved; and thirdly, wood is replaced with bamboos and bran is replaced with the biogas residues, so that the source of a cultivating raw material is widened, and the production cost is reduced. The mushroom cultivating material has the social benefits that wood dust is replaced with the bamboo sawdust and the bamboo shells, so that part of contradiction between a fungus and forestry can be alleviated, waste materials can be changed into things of value, and energy conservation and emission reduction can be favorably realized.

The invention discloses a fruit jam with rose and aloe which comprises the following components in parts by mass: 30-40 parts of apples, 30-45 parts of pears, 40-50 parts of peaches, 50-60 parts of tomatoes, 15-20 parts of raisin, 20-25 parts of red dates, 10-15 parts of medlar, 10-15 parts of rose pollen, 15-20 parts of aloe, 15-20 parts of white sugar and 5-8 parts of vinegar. A preparation method of the fruit jam with rose and aloe comprises the following steps of: chopping apples, pears, peaches, tomatoes, red dates, medlar and aloe into less than 2*2 mm small pieces, decocting together with raisin, rose pollen, white sugar and vinegar for 3-5h at 100-120 DEG C, cooling to 80-90 DEG C, decocting for 2-3h to obtain the fruit jam with rose and aloe. The fruit jam with rose and aloe contains the nutrient components of various fruits, has health care efficacy and is a composite fruit jam with tasty and refreshing mouth feel.

The present invention relates to a soil-less sand based root zone medium for the production of sod. The present invention is also directed to a method for the production of sod using a soil-less sand based root zone medium and a sod mat produced by the method of the present invention.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a chtiosnaoligosaccharide (COS) capsule and a preparation method thereof. The COS capsule comprises a content and a capsule shell. The content is mainly prepared from COS, microcrystalline cellulose, adhesive, calcium stearate, ethyl alcohol and superfine silica powder. The capsule shell is mainly prepared from hydroxypropyl starch, xanthan gum, a thickening agent, a plasticizer and water. The COS capsule is free from extraneous odor, and clean, tidy and smooth in appearance; both the weight deviation and the solubility of the COS capsule meet the related stipulations of Chinese pharmacopoeia. In addition, the capsule has good leakproofness and oxidation resistance, the problem that oxidation and moisture absorption can happen to COS easily is effectively solved, and long-term storage of the COS is facilitated.

The invention provides a freshwater mussel meat-lotus root seasoning for instant noodles. The freshwater mussel meat-lotus root seasoning for instant noodles is prepared from the following raw materials in parts by weight: 70-80 parts of freshwater mussel meat, 50-60 parts of red wine, 40-50 parts of ginger juice, 60-70 parts of lotus root, 40-50 parts of hawthorn, 70-80 parts of honey, 100-120 parts of pineapple juice, 50-60 parts of walnut kernel, 7-8 parts of rose flowers and the like. The freshwater mussel meat-lotus root seasoning for instant noodles is delicious in taste due to the added freshwater mussel meat, is capable of promoting appetite and clearing heat due to the added hawthorn and lotus root, and also has certain effects of maintaining beauty, invigorating stomach and the like due to the added extracts of rose flowers, medlar, radix rehmanniae, gooseberry and the like.

Methods, reagents, compositions, and kits for in situ interaction determination (ISID) via interaction-dependent polymerase chain reaction (ID-PCR) are provided herein. ISID technology is useful for rapidly evaluating potential small molecule-target interactions from mixtures in a single solution. ISID is compatible with unpurified targets in biological samples and can be used to evaluate ligand-binding in DNA-encoded chemical libraries in cell lysates. ISID is also useful to screen ligand interactions of proteins or other molecules in their native state, including their native post-translational modifications and any interactions with accessory proteins and metabolites, in ways that better reflect their relevant biological environment. Because ISID is compatible with crude cell lysates, difficult-to-purify, poorly soluble, intrinsically unstable, and aggregation-prone targets may also be compatible with this method, without requiring truncation or other strategies used to promote heterologous expression.

The invention discloses a quantitative detection method for FOC (Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyd. & Hans.) race 4 from the soil. The method comprises the following steps: pretreating a sample to be detected and extracting DNA of the sample to be detected; preparing specific primers; conducting fluorescent nucleic acid isothermal amplification detection technique RealAmp reaction; constructing a RealAmp standard curve; and determining the result. The real-time fluorescent nucleic acid isothermal amplification detection technology provided by the invention is a novel nucleic acid detection technology combining a new generation of isothermal nucleic acid amplification technology with the real-time fluorescent detection technology, and has the advantages of high sensitivity, high specificity, low pollution and stable reaction. RealAmp can quantitatively detect pathogens; because of the characteristics of LAMP, Bst DNA polymerase has chain exchange capacity and good tolerance of inhibiting substances contained in the soil DNA extracts; the method carries out detection work on the soil, avoids cover opening and influence by contaminants and conducts on-site soil sample detection; the method for analysis and determination of reaction products is very simple; therefore, the method is suitable for wide application.

The invention provides a method for determining the physical form of a fibrous root plant. The method comprises the following steps: cleaning the root of a plant; sectioning the root of the plant after cleaning; separately sampling each section of the root and scanning the samples in a scanner to obtain root images; drying the samples and the rest of the root and separately weighing the samples and the rest of the root; and analyzing the root images by using a root image analysis software to obtain the root length, root volume and surface area of each sample of each section of the root. According to the invention, the method can reduce the time for scanning the root and provide complete data.

The invention discloses an odor identification method based on gas sensors and deep learning, which is characterized in that a response curve cluster of odor to be detected is obtained through a gas sensor array, original data is directly used as an input sample of an odor identification deep neural network, data preprocessing and data amplification are performed on the input sample, time sequenceresponse data hierarchical features are automatically extracted by adoption of deep learning, global feature extraction and long-range dynamic feature extraction are performed at the same time, and an odor label is output through a classifier, so that high-sensitivity and specific odor identification is realized. The odor identification method of the invention has high sensitivity and high reliability, and can be widely applied to the fields of industrial production, medical treatment, environment, safety and the like.

The invention discloses a 1,3-1,4-beta-glucanase mutant, and belongs to the fields of gene engineering and enzyme engineering. Lysine in the 20th position, 117th position and 165th position of 1,3-1,4-beta-glucanase from Bacillus terquilensis mutates through an overlapping extension PCR method to form serine in order to obtain single mutants K20S, K117S and K165S respectively. The above three mutation sites are integrally mutated to obtain a K20S/K117S/K165S triple mutant enzyme. Four mutant enzymes have higher catalysis activity and better thermal stability. Compared with wild enzymes, the above mutant enzymes are in favor of realizing the industrial application.

The invention provides a soil bio-activator for ginseng continuous cropping and belongs to the field of modernization of Chinese traditional medicine. The soil bio-activator for ginseng continuous cropping can be used for solving the problems of serious ginseng continuous cropping diseases, low yield and poor quality caused by deterioration of the physicochemical and biological characters of old ginseng-grown land soil. The soil bio-activator for ginseng continuous cropping is prepared from 2.0%-8.0% of bacillus subtilis, 2.0%-8.0% of actinomycetes, 5.0%-20.0% of xylanase, 5.0%-20.0% of cellulase, 10.0%-20.0% of urease, 1.0%-4.0% of potassium dihydrogen phosphate, 0.1%-0.5% of ferrous sulfate, 0.1%-0.4% of boric acid, 0.1%-0.5% of manganese sulfate, 0.05%-0.2% of copper sulfate, 0.1%-0.4% of zinc sulfate, 0.05%-0.2% of ammonium molybdate and the balance of organic materials through mixing and compositing. The soil bio-activator for ginseng continuous cropping is used for improving the soil for ginseng continuous cropping in ginseng growing.

The invention aims to provide a microfluidic chip system for culture and multiplication behavior research of marine microalgae. Inoculation, batch culture, semi-continuous chemostat culture of the marine microalgae and a marine microalgae population multiplication behavior online monitoring process are integrated on a functional chip to be completed by means of fabrication of a scale integration chip and flexible combination of multiple cell technologies on the chip. The microfluidic chip system consists of two elementary units, wherein the first elementary unit refers to the microfluidic chip for culture and multiplication behavior research of the marine microalgae, and the second elementary unit refers to microfluidic chip peripheral equipment for culture and multiplication behavior research of the marine microalgae. The microfluidic chip system has the advantages that the microfluidic chip system is high in throughput, simple and convenient to operate and low in cost and is a novel platform for marine microalgae culture, population multiplication research and correlational research fields of using microalgae as experimental subjects.

The present invention provides data to demonstrate that the re-fusion, of a mammalian karyoplast to an enucleated in vivo ovulated cocyte, following an unsuccessful initial simultaneous electrical fusion and activation event offers an additional alternative and improvement in the creation of activated and fused nuclear transfer-capable embryos for the production of live off-spring in various mammalian non-human species including goats, pigs, rodents, primates, rabbits and cattle. Additionally, multiple electrical pulses offers an alternative and more efficient activation method in a simultaneous fusion and activation methodology for viable offspring production in a animal nuclear transfer program.

The invention belongs to the field of molecular biology and discloses a serum/plasma miRNA composition and use thereof. The miRNA composition comprises one or more of the following miRNAs: hsa-miR-122, hsa-miR-92a, hsa-let-7c, hsa-miR-23a, hsa-miR-23b, hsa-miR-223, hsa-miR-342-3p, hsa-miR-423-5p, hsa-miR-375, hsa-miR-99a, hsa-miR-150, hsa-miR-125b and hsa-miR-10a. The miRNA composition screened out by the invention and the primers of the miRNA composition have much higher specificity and flexibility for detecting HBV-positive people and HBV-positive patients with hepatocellular carcinoma than protein markers currently used in clinic, and therefore can greatly improve the accuracy of diagnosis. The miRNA composition and the primer composition thereof both can be used in the preparation of reagents and tools for detecting HBV infection and/or HBV-positive hepatocellular carcinoma.

The invention relates to a preparation and using method of a vibrio vulnificus rapid detection kit; wherein, the interior of the kit includes a loop-mediated isothermal amplification reaction liquid A, a Bst DNA polymerase B and a chromogenic agent C; the reaction liquid A contains a reaction buffer liquid, a dNTP, a magnesium sulfate, an upstream internal primer 5-ACCTGTGTTCCATGCTGCTTCTATTCGCAACTGACATGTCGG-3, a downstream internal primer 5-TAACAAAGGTCACCGTCCAGGCCAGAACGCAGGTCTTGTGAA-3, an upstream external primer 5-CCAGAGCCAGAGTTCTTCCT-3, a downstream external primer 5-GGCCCATCTCTTCCATCAC-3 and a betaine; the method for detecting the vibrio vulnificus includes the DNA extraction of the samples or the bacteria to be detected, the loop-mediated isothermal amplification reaction of the vibrio vulnificus and the chromogenic detection. The preparation and using method has the advantages of swiftness, strong specificity, high sensitivity and low cost.