114 results about "Fermentation" patented technology

The invention relates to a method for producing an amino acid tobacco-specific foliar fertilizer by using abandoned fur. The method comprises the following steps of: degreasing, drying and crushing various abandoned animal hair in slaughterhouses, hair in barber shops and various abandoned animal leather which are used as main raw materials; carrying out microbial fermentation, mainly Freund crack mould or other keratin decomposing bacteria fermentation; then decomposing and filtering to prepare a crude amino acid solution; and chelating the following components in parts by weight: 30-50 parts of crude amino acid solution, 15-18 parts of potassium sulfate, 15-25 parts of ammonium sulfate, 15-18 parts of potassium dihydrogen phosphate, 3-6 parts of humic acid potassium, 1.3-2 parts of trace elements, 0.3-0.5 part of surfactant, 0.5-1 part of chelating agent and 0.15-0.2 part of plant growth regulating agent. The tobacco-specific foliar fertilizer has the effects of promoting growth of tobacco roots, enhancing the absorption and utilization of tobacco on soil and the fertilizer, promoting lengthening, widening and thickening of tobacco, enhancing the ability of disease resistance, and improving the quality and yield of tobacco leaves.

The invention discloses a method for preparing an organic fertilizer by fermenting cow dung compost. According to the method, two times of composting fermentation are adopted and a composting promoter is added, so that the fermentation speed and the composting degree can be quickly increased; meanwhile, polyacrylamide, bentonite, cocoanut active charcoal and the like are added to reduce the moisture in cow dung and achieve the effect of deodorizing; sodium bicarbonate and triple superphosphate are added to generate carbon dioxide which can expand the cow dung, so that the permeability is increased; the early-stage fermentation temperature of the cow dung can be increased by the heat released from reaction, so that the activity of natural fermentation bacteria agents in the cow dung is improved; meanwhile, when the heat generated by the cow dung after a period time of composting is overhigh, the polyacrylamide absorbs the moisture in the cow dung to achieve the effect of reducing the temperature and to reduce turning times; after the fermentation is completed, the organic fertilizer prepared by drying and dewatering is high in fertilizer efficiency, durable and efficient, has the effects of retaining water, sterilizing and improving soil, and can promote the rapid growth of crops, thus improving the yield and the quality of the crops.

The invention relates to an intelligent bread machine. The intelligent bread machine comprises a body, an upper cover, a bread bucket and a main controller, wherein bottom feet are arranged at the bottom of the body; a water pump, a temperature detection device, a heating device and a stirring device which are electrically connected with the main controller are arranged in the bread machine; the main controller comprises a motor control unit connected with the stirring device and a weight acquisition device arranged at one of the bottom feet of the body and used for acquiring a weight signal of the bread machine; the main controller is provided with a weight calculation unit electrically connected with the weight acquisition device, a water addition amount calculation unit electrically connected with the water pump as well as a fermentation calculation unit and a heating calculation unit which are electrically connected with the heating device; the weight calculation unit is used for receiving the weight signal from the weight acquisition device and calculating a weight difference before and after flour is added; and the water addition amount calculation unit, the fermentation calculation unit and the heating calculation unit are controlled to calculate the corresponding water addition amount, the fermentation time, the fermentation temperature, the heating time and the heating temperature according to the set programs by utilizing a signal of the weight difference.

The invention relates to an edible mushroom, spent mushroom substrate and probiotics-containing nutrient solution for livestock and poultry and a preparation method thereof. The preparation method comprises the following steps of: preparing leach liquor by using waste spent mushroom substrates as raw materials, extracting a plurality of active ingredients from the spent mushroom substrates, and then fermenting and preparing the nutrient solution containing a plurality of microorganisms beneficial for the growth of the livestock and poultry by using the leach liquor as a culture medium. The prepared nutrient solution is brown in color and tart, slightly has the incenses of edible mushrooms, and contains abundant probiotics groups so as to be beneficial for enhancing the appetite of the livestock and poultry and promoting the absorption of nourishment and the growth of the livestock and poultry. Compared with the solid fermentation of the conventional preparation method of spent mushroom substrate-containing feeds, due to the adoption of the main production method of liquid fermentation, the preparation method of the nutrient solution has the advantages of environmental protection, energy saving and convenient operation; and the obtained product is liquid so as to have the advantages of convenient application and favorable palatability for the livestock and poultry.

The invention discloses a pollution-free paddy rice planting method comprising following steps: seed pretreatment: paddy rice seeds are successively subjected to soaking in a soak solution, voltage application in a corona field and mixing to obtain paddy rice seeds for sowing; 2. land preparation: pig dung layer, three different bio-fertilizers and marsh manure layer are successively laminated and embedded in multiple trenches of soil and fermentation on-site is performed to obtain fertile seedling growing bed; 3. seedling growing and self-made nutrient soil: seedling growing holes of a seedling growing tray is filled with the nutrient soil, the paddy rice seeds for sowing are spread in the seedling growing holes, watering and fertilization management are performed to obtain seedlings to be transplanted; 4. transplanting. According to the method of the invention, through pretreatment of paddy rice seeds and land preparation of rice seedling beds, fertile seedling growing beds which can prevent insect disease are obtained to increase the germination rate and seedling emergency rate of paddy rice, improve the quality of rice seedlings and make the seedlings robust and root well developed; the seedlings are provided with strong insect disease-resistant ability; high quality and pollution-free paddy rice is obtained.

The invention discloses Escherichia coli and a method for preparing L-cysteine by using the same. Escherichia coli BL21(DE3) is used as a template for the genome of the Escherichia coli, and the template has serine acetyltransferase shown as a sequence in SEQ ID NO.1 and in deletion of a gene metC, a gene tnaA, a gene gshA and a gene dfp. The method comprises the following steps of: firstly, putting the Escherichia coli in a seed culture medium for culturing; and then putting the Escherichia coli in a fermentation culture medium to ferment to obtain the L-cysteine. The invention has the advantages that by utilizing the Escherichia coli to prepare the L-cysteine, the conversion rate of a substrate and the output of the L-cysteine are greatly improved; the conversion rate of the substrate can reach 30 percent improved by more than 50 percent compared with that of the prior art; and the output of the L-cysteine can be improved by more than 20 percent compared with that of the prior art.

The invention relates to a coal mine omnipotent gas extraction agent, in order to solve the problem of poor gas extraction effect in existing coal mines. The extraction agent comprises the following raw materials according to weight ratio: 100 of water-soluble organic acid and 3-28 of sugar. The weight ratio of raw materials is 100 of water-soluble organic acid and 3-28 of sugar, and the extraction agent further comprises water of which the weight is 5-18 times as that of the above raw materials. The water-soluble organic acid is selected from fulvic acid, acetic acid, complex acid obtained from plant fermentation, citric acid and lactic acid; the sugar is selected from maltose, sucrose, fructose, konjac powder and agar. The extraction agent has the advantages of low cost, easy operation and use, no influence on coal quality, good effect, ability of improving the gas extraction effect of coal mines dramatically, no obvious influence on underground operation staff and equipment, high security and high efficiency.

The invention discloses a method for lowering the content of nitrogen metabolism hazard materials in soy sauce through compound bacteria, and belongs to the technical field of microorganisms. By meansof combined fermentation of staphylococcus xylosus and Tetragenococcus halophilus, by lowering accumulation of biogenic amine and ethyl carbamate, the nitrogen metabolism hazard materials in soy sauce are controlled. In the soy sauce fermentation process, when soy sauce is mixed with finished koji and saline water according to the ratio of 1:1, when staphylococcus xylosus and Tetragenococcus halophilus are inoculated at the same time for fermentation, the total biogenic amine content in the finished soy sauce can be lowered by 64.2%, the content of ethyl carbamate is lowered by 77.2%, and meanwhile the amino nitrogen is increased by 61.1%.

The present invention utilizes water source heat pump technology in beer fermenting tank cooling step. The water source heat pump set has one side connected to the beer fermenting tank so as to cooling the fermenting tank with the heat pump refrigerator, and other side connected to boiler water replenishing tank so as to preparing hot water at 50 deg.c with the heat pump condenser. The hot water may be used to provide hot water to other step and provide water to boiler oxygen eliminator. The water source heat pump technology can utilize full heat and save energy at most.

The invention belongs to the technical field of prevention and treatment of plant diseases and relates to a bacillus amyloliquefaciens strain capable of preventing and treating wheat scab and application thereof. The preservation number of the separated bacillus amyloliquefaciens S76-3 is CCTCC NO:M2014315, and nucleotide of 16S rDNA of the separated bacillus amyloliquefaciens S76-3 is shown in SEQ ID NO:1. The invention discloses separation, identification and broad-spectrum bacteriostasis of the bacillus amyloliquefaciens, spore yield fermentation optimization of the bacillus amyloliquefaciens and field application of the bacillus amyloliquefaciens in prevention and treatment of wheat scab. The liquid inoculant of the bacillus amyloliquefaciens fermentation diluent can be used for effectively inhibiting the wheat scab and harm in the field and reducing toxins in wheat ears. The bacillus amyloliquefaciens strain has strong stress resistance and does less harm to the environment, so that the bacillus amyloliquefaciens strain can be taken as an efficient antibiological inoculant; and the bacillus amyloliquefaciens is broad in antibacterial spectrum and can be used for inhibiting growth of ten plant pathogenic fungi including fusarium graminearum, so that the bacillus amyloliquefaciens strain has a good development and application prospect.

The invention provides a method for rapidly optimizing metal ions to promote bacillus to produce spores by a response surface method. The method comprises the following steps: taking spores DPA fluorescence intensity as a response value; screening metal ions which possibly affect fermentation of spores by a simple factor design of experiments at first; and selecting types of metal ions which remarkably improve the fluorescence intensity and the most suitable adding concentration when the concentration of the ions is changed. A central combined design is adopted further, the fluorescence intensity of spores DPA is also used as a response value, a combined effect of the metal ions is counted, analyzed and determined through a response surface, and thus, maximization of the yield of spores under the combined condition of a certain concentration of the ions is implemented. By the method, the working strength of detection of the test design in a fermenting process of the spores can be greatly reduced, a test period is shortened, test accuracy is ensured, and the design efficiency is improved.

The invention relates to the technical field of food detection and particularly relates to a black tea fermentation quality index detection method based on electrical characteristic parameters. The detection method comprises the steps of respectively putting different fermentation degrees of tea into an electric characteristic testing system to measure electric characteristic parameters of parallel-connection equivalent capacitance, complex impedance, resistance, reactance, loss factors, phase position loss angles and the like; detecting quality indexes of sense grade, theaflavin, thearubigins, theabrownin and the like at the same time; establishing a database by utilizing the electric characteristic parameters and the quality indexes; performing feature electric characteristic parameter screen on the data in the database; establishing a black tea fermentation quality prediction model; utilizing an appropriate relative standard deviation value as an index of prediction capacity of the model. The detection method disclosed by the invention overcomes the defects of manual sense evaluation and physical-chemical detection, improves objectivity and precision of black tea fermentation quality evaluation, achieves automatic, intelligent and precise fermentation control and shows very good practical significance and application prospect in tea quality index detection.

The invention provides a method for preparing health-maintaining liquor from longan, phyllanthus emblica, olive, hawthorn and passion fruit. The method comprises the steps of: adding a yeast with flocculation optimization characteristics and a fermentation assistant into longan juice for fermentation; and then adding phyllanthus emblica juice, olive juice and/or hawthorn juice to obtain the health-maintaining wine.

The invention belongs to the field of microbial genetic engineering, and discloses a GTP cyclohydrolase I gene folE and application. A nucleotide sequence of the GTP cyclohydrolase I gene folE is shown as SEQ ID NO:1. The gene is derived from food-borne lactobacillus plantarum, is safe and can be used in the field of later-stage food fermentation. A gene knockout strain screening method can be used for improving the screening efficiency of knockout strains. The key role of gene folE gene in folic acid synthesis is proved, and a certain theoretical basis is provided for the research and development of folic acid synthesis functional food. The GTP cyclohydrolase I gene folE and application analyze that the gene is derived from food-grade microorganisms and is safe; and the GTP cyclohydrolaseI gene folE and application analyze that the gene folE gene has nothing to do with cell morphology and strain growth except playing an important role in folic acid synthesis.

A method for anaerobic digestion of easily acidified wastewater (substance) to produce methane comprises the following steps: (1) directly adding the easily acidified wastewater into a fully mixed anaerobic digestion reactor as a fermentation substrate; (2) adding the inoculum into a fully mixed anaerobic digestion reactor; (3) adding granular activated carbon into a fully mix anaerobic digestionreactor; (4) carrying out anaerobic digestion reaction, and before that reaction proceeds, introducing nitrogen gas into the reactor to ensure that there is no oxygen in the system; Then at the speedof 100r/min and the temperature of 30-50 DEG C, carrying out reation. During the process of the reaction, the pH value does not need to be adjusted, and the time of anaerobic digestion is controlled to be 5-20 days. Methanogenesis of easily acidified wastewater (substance) was realized by adding granular activated carbon into digested substrate, which was produced in food processing industry, vegetable processing industry and catering industry, and then the acidified wastewater (substance) was digested.

The invention relates to fields of pesticides and bacterial inhibitors, which particularly relates to an ophiobolin di-sesquiterpene compound derived from seaweed entophytic fungi, and preparation and application thereof. The structural formula is shown in a formula (I). The preparation method comprise: inoculating Aspergillus ustus cf-42 in a fungus liquid medium to ferment and culture. After purification, the fermentation product is the ophiobolin sesterterpene compound as shown in the formula (I). The ophiobolin di-sesquiterpene compound obtained by the invention can cause a higher death rate of artemia salina sinnaeu which is 78.4% when the compound concentration is 100 microgram/ml. An insecticidal activity experiment suggests that the median lethal concentration is 48.1 microgram/ml. At the meantime, this compound also possesses substantial bacteriostatic activity.

The invention provides a processing method of immature xinhui citrus peel filled with Liupao tea, and belongs to the technical field of tea leaf processing. The processing method comprises the steps of preparing semi-finished Liupao tea and preparing the immature xinhui citrus peel filled with Liupao tea, wherein the step of preparing the semi-finished Liupao tea comprises the steps of performingfresh leaf fixation, performing primary twisting, performing heaping for fermentation, performing secondary twisting and performing drying; and the step of preparing the immature xinhui citrus peel filled with Liupao tea comprises the steps of treating small green citruses, loading tea, performing primary steaming, performing cold fermentation, performing secondary steaming and performing ageing.The processing method disclosed by the invention is simple in technology, easy to operate and high in production practicality; and according to the method disclosed by the invention, high quality andhigh efficiency of processing of the immature xinhui citrus peel filled with Liupao tea can be guaranteed, particularly the color, fragrance, taste, and shape and the quality of tea grounds of the immature xinhui citrus peel filled with Liupao tea can be improved, the yield for making tea is increased, and the quality of the immature xinhui citrus peel filled with Liupao tea can be improved.

The invention relates to a making method of a high-protein fermented feed, belongs to the technical field of feed processing, and particularly relates to a making method of the high-protein fermented feed. The making method comprises the following steps of weighing a certain mass of linseed meal, adding sprouted cargo rice powder and ammonium sulfate, adding water and performing uniform mixing so as to obtain a solid culture medium; and enabling the solid culture medium to be subjected to enzymolysis, sterilization, fermentation, low temperature drying, crushing, granulation and packaging, so as to obtain finished products. The high-protein fermented feed disclosed by the invention has the advantages of being rich in nutrition, high in protein content, and good in palatability. In addition, the step of enzymolysis is performed before fermentation, so that nutrition resistant factors having toxicity in the linseed meal are decomposed and released, the eating safety is improved, nutrient substances having health-care efficacies, such as gamma-aminobutyric acid and flavonoid glycosides are also generated, and the health-care efficacies of the fermented feed are improved.

The invention discloses a preparation method of a tomato red sour soup. The method comprises the following steps: (1) preparing a fermentation liquid: adding rice wine to wheat flour, and conducting fermentation at 27-37 DEG C for 15-21h to obtain the fermentation liquid; (2) preparing raw materials: cleaning fresh tomatoes, cutting the tomatoes into blocks with a width and length of 0.5-1.1cm, and cleaning and chopping fresh red chilies; (3) conducting decocting: adding pungent litse fruit oil, hot pickled mustard tuber strips, raw ginger powder, garlic powder and purified water to the tomatoblocks and the chopped red chilies, conducting decocting until the mixture is in a thick state, and cooling the mixture for later use; (4) mixture materials: mixing the thick mixture with the fermentation liquid, water-milled glutinous rice flour, salt and rice wine with stirring so as to obtain a new mixture; and (5) conducting fermentation: fermenting the new mixture at 22-26 DEG C for 17-21 days to obtain the tomato red sour soup. With the preparation method of the tomato red sour soup provided by the invention, the prepared tomato red sour soup is denser and more fragrant in taste and characteristic in taste, has a long preservation time, and is suitable for mass production.