The invention provides a purification method of
teicoplanin, and the purification method comprises the following steps: (1) adjusting
teicoplanin fermentation liquid to be alkaline, then performing
filtration; (2) adjusting the filtrate obtained in step (1) to be acidic, eluting the filtrate through macroporous adsorption resin by using water and
ammonia water in order, collecting the
ammonia water eluate; (3) filtering the eluate obtained in step (2), separating the filtrate through
silica gel chromatography, collecting the eluate of
ethanol aqueous solution with a volume ratio of 10-50%; (4) adjusting the eluate obtained in step (3) to be acidic, performing
ultrafiltration by an ultrafilter membrane with a cutoff molecular weight of 5000-30000 Da, preferably 10000 Da, so as to obtain an ultrafiltrate; and (5) performing
nanofiltration of the ultrafiltrate obtained in step (4) through a
nanofiltration membrane with a cutoff molecular weight of 100-1000 Da, preferably 200 Da, and concentrating to obtain the
teicoplanin. The purification method of the invention improves the production efficiency of teicoplanin to the maximum extent, and thus realizes industrial production of high-purity teicoplanin.