88results about "Peptide preparation methods" patented technology

The present invention relates to molecules, particularly polypeptides, more particularly immunoglobulins (e.g., antibodies), comprising a variant Fc region, wherein said variant Fc region comprises at least one amino acid modification relative to a wild-type Fc region, which variant Fc region binds FcγRIIIA and/or FcγRIIA with a greater affinity, relative to a comparable molecule comprising the wild-type Fc region. The molecules of the invention are particularly useful in preventing, treating, or ameliorating one or more symptoms associated with a disease, disorder, or infection. The molecules of the invention are particularly useful for the treatment or prevention of a disease or disorder where an enhanced efficacy of effector cell function (e.g., ADCC) mediated by FcγR is desired, e.g., cancer, infectious disease, and in enhancing the therapeutic efficacy of therapeutic antibodies the effect of which is mediated by ADCC.

The present invention provides a monoclonal anti-idiotype antibody 11D10 that elicits an immune response against a specific epitope of a high molecular weight mucin of human milk fat globule (HMFG) and a hybridoma that produces 11D10. The hybridoma that produces 11D10 was selected by specific procedures. 11D10 induces an immunological response to HMFG in nice, rabbits, monkeys and patients with advanced HMFG-associated tumors. This invention provides compositions derived from polynucleotide sequences encoding the variable light and/or variable heavy regions of monoclonal anti-idiotype antibody 11D10, as well as polypeptides encoded thereby. The invention also provides compositions which can be used in the detection or treatment of HMFG-associated tumors.

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

Provided is a filler for affinity chromatography having a high dynamic binding capacity for proteins and having excellent alkali resistance and storage stability. The filler for affinity chromatography of the present invention comprises a porous particle consisting of a copolymer of 40 parts to 99.5 parts by mass of (M-1) a methacryloyl group-containing vinyl monomer that contains a hydroxyl group and does not contain an epoxy group, 0.5 parts to 30 parts by mass of (M-2) an epoxy group-containing vinyl monomer, 0 parts to 59.5 parts by mass of (M-3) a methacryloyl group-containing vinyl monomer which is other than the monomers (M-1) and (M-2), and 0 parts to 25 parts by mass of (M-4) a vinyl monomer other than the monomers (M-1), (M-2) and (M-3) (with the proviso that the total amount of the contents of (M-1), (M-2), (M-3) and (M-4) is 100 parts by mass); ring-opened epoxy group obtainable by ring-opening of epoxy group that is contained in the copolymer; and ligand that is bound to the porous particle.

A semi-recombinant method for the production of GLP-1 analogues and derivatives with non-proteogenic amino acids in the N-terminal part combining the use of recombinant expression techniques and chemical peptide synthesis.

The present invention provides novel conformationally-defined macrocyclic compounds that have been demonstrated to be selective modulators of the ghrelin receptor (growth hormone secretagogue receptor, GHS-R1a and subtypes, isoforms and variants thereof). Methods of synthesizing the novel compounds are also described herein. These compounds are useful as agonists of the ghrelin receptor and as medicaments for treatment and prevention of a range of medical conditions including, but not limited to, metabolic and/or endocrine disorders, gastrointestinal disorders, cardiovascular disorders, obesity and obesity-associated disorders, central nervous system disorders, genetic disorders, hyperproliferative disorders and inflammatory disorders.

The invention discloses a synthesizing method of sermaglutide. The synthesizing method comprises the following steps of step 1, preparing Fmoc-Gly-Wang resin; step 2, using the Fmoc-Gly-Wang resin, protective amino acids, and octadecandioic acid (OtBu)-gamma-Glu-OtBu-PEG-PEG-OH as the raw materials to prepare full-protective peptide resin which contains Depsipeptide Units; step 3, performing TFA (trifluoroacetic acid) cracking on the peptide resin, so as to obtain crude peptide; step 4, dissolving the crude peptide obtained in step 3, adjusting the pH (potential of hydrogen) value, and converting ester bonds into amide bonds to react, so as to obtain a crude product of the sermaglutide. The synthesizing method has the advantages that by introducing the Depsipeptide Units, the problem of difficulty in sequential synthesizing of the sermaglutide is solved, and the target product is obtained by the reaction of converting the ester bonds into the amide bonds; the purity and yield of the crude peptide are improved, and the synthesizing method is suitable for downstream purifying, and is suitable for industrialized production.

The present invention relates to a safe non-virulent Yersinia enterocolitica mutant strain for delivering heterologous proteins in target cells carrying mutations in at least one of the effector genes yopH, yopO, yopP, yopE, yopM, yopT genes and at least one additional mutation in the invasin genes chosen from yadA and/or inv. The present invention also relates to a safe non-virulent Yersinia enterocolitica mutant strain for delivering heterologous proteins in target cells according to claim 1 carrying mutations in all effector genes yopH, yopO, yopP, yopE, yopM, yopT genes and at least one additional mutation in the invasin genes chosen from yadA and/or inv. The present invention also relates to an expression vector for delivering a heterologous protein into a target cell using a Yersinia enterocolitica mutant strain according to any of the claims 1 to 4, which comprises in the 5' to 3' direction :(a) a promoter of a Yersinia virulon gene, (b) a first DNA sequence encoding a delivery signal from a Yersinia effector protein, operably linked to said promoter; and, (c) a second DNA sequence coding for said heterologous protein, fused in frame to the 3' end of said first DNA sequence. The present invention further relates to methods and compositions comprising (the use of) the afore-mentioned mutant strains and expression vectors.

The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to novel human secreted IGFBP-like polypeptides. Other aspects of the invention include vectors containing processes for producing novel human secreted IGFBP-like polypeptides, compositions, and methods of use for such polypeptides including in cancer.

Methods for forming maleimide functionalized polymers are provided. In one such embodiment, a maleimide functionalized polymer is prepared in a method that includes a step of carrying out a reverse Diels-Alder reaction. Intermediates useful in the methods, as well as methods for preparing the intermediates, are also provided. Also provided are polymeric reagents, methods of using polymeric reagents, compounds and conjugates.

Methods of enhancing immune responses in which soluble forms of costimulatory molecules, e.g., B7 molecules, are administered to augment immune responses to antigens, e.g., to tumor cells and infectious agents are provided. The subject methods are useful for both prophylactic and therapeutic immunization of subjects.

The present invention relates generally to microtiter plate format devices and methods for separating molecules having different net charges. The devices and methods of the invention are particularly suited for use in high-throughput screening to monitor enzymatic reactions which result in a product having an altered net charge. For example, the systems and methods disclosed herein may be used to detect the activity of phosphatases, proteases and kinases on various peptidic substrates under various conditions.

The invention belongs to the technical field of immunological analysis, in particular to an ELISA method for detecting doxycycline (DOX) remnant and a kit. The method mainly comprises the preparation of immunogen, coatingen and antibody, the pretreatment of the sample and the establishment of the ELISA detection method. The kit of the invention mainly comprises anti DOX specific antibody, a DOX standard substance, and an elisa plate coated with DOX-OVA conjugate. The kit and the method of the invention are characterized by simplicity, convenience, high speed, sensitivity, accuracy and the like and are suitable for detecting DOX remnant in animal edibility tissue.

The invention relates to a preparation method for recombinant human horny cell growth factor, special to prepare biological active polypeptide, which comprises: a. synthesizing artificially the gene of amino acid coding region of objective product; b. fusing the obtained gene with proper 3' end of S- transferase protein gene of GSH on expression carrier contained thrombin or enterokinase recognition site; c. conversing obtained expression carrier into proper escherichia coli to obtain genetic engineering fungus; d. fermenting the fungus, extracting, chromatography, purifying, and obtaining the GST-KGF fused protein; e. cutting by thrombin or enterokinase, separating and purifying, and obtaining the objective factor.

A process for preparing the active embryonic peptide with coproduct of corn oil from the embryo bud of corn at the same time includes such steps as wet and thermal pretreating, grinding by colloid mill, multi-stage zymolysis, centrifugal separation to obtain fibres, corn oil and milk, concentrating the milk by nanometre filtering membrane, further concentrating, homogenizing, emulsifying, drying and granulating.

A novel protein having inhibitory activity on the protease activity of hepatocyte growth factor (HGF) activator was purified and isolated, and its molecular weight (ca. 40,000 daltons) and its N-terminal and partial amino acid sequences were determined. A gene coding for the protein was cloned, and the gene DNA was incorporated into a vector, for transforming host cells. Cultivation of the transformant gave the desired protein. The protein can be used as an in vivo or in vitro control factor for HGF or HGF activator. It is also useful as an antigen to be used in producing an antibody to be used as means for kinetic studies of the protein, or as a standard in assay systems therefor.

The invention discloses a preparation method of a medical and edible fungus mycelium polysaccharide-polypeptide immune enhancer. The preparation method comprises efficient extraction of mycelium polysaccharide polypeptide based on combination of a physical method and enzymatic hydrolysis, ethanol-based precipitation separation, precipitation purification respectively with levamisole and high-concentration ethanol, and blending preparation of compound immunity-enhancing injection preparations. Through mixing with a vaccine or compounding with an injection, animal immunity is improved, production efficiency is high and a relative cost of production is low. The lactarius deliciosus mycelium polysaccharide is simpler than lactarius deliciosus mycelium monosaccharide and can be further decomposed into rare functional monosaccharide. The preparation method has simple processes.

A simple and accurate separation purification method for object biomolecules or bio-associated substance through a particulate aggregative reaction is provided. A microfluidic circuit utilizing a particulate aggregate as a detection marker in a nonfluorescence method such as an electrochemical method or surface plasmon resonance is provided. The separation purification method comprises the steps of forming a particulate aggregate by a reaction between particulates modified with a labeling substance specifically reacting with a label object substance and biomolecules or a bio-associated substance containing the label object substance and separation-purifying the particulate aggregate. The microfluidic circuit utilizes this separation purification method.

The present invention provides transgenic mice deficient in corticotropin releasing factor receptor 2 (CRFR2). Mice deficient for CRFR1 exhibit decreased anxiety-like behavior and a decreased stress response. In contrast, CRFR2 null mutant mice are hypersensitive to stress and display increased anxiety-like behavior. These mice are useful for the study of anxiety, depression, and the physiology of the HPA axis. CRFR2 null mutant mice also exhibit increased angiogenesis in all tissues examined. Thus, CRFR2 antagonists may be used to stimulate angiogenesis for the treatment of various conditions. In contrast, CRFR2 agonists may be used to inhibit angiogenesis. A combination of urocortin and bFGF was observed to stimulate rapid hair growth.

The invention discloses an efficient preparation method of low-value fish immunomdodulating peptides, relates to the development and utilization of low-value fish bioactive peptides and related anti-inflammatory drug products, and belongs to the technical field of low-value fish processing; the preparation method comprises the steps of preparing nemipterus virgatus myosin by a salt extraction method, fully expanding a high-spiral structural domain of myofibrillar protein by using a high-pressure homogenization method; fine-tuning a protein solution to pH 7.5, adding trypsin, performing enzymatic hydrolysis for 90 minutes, promoting an ultrafiltration membrane to separate a nemipterus virgatus protein enzymatic hydrolysate by using impressed current, and obtain a peptide fragment of 500 to3000 components; finally obtaining a powdery product by a freeze-drying method; the method can improve the degree of hydrolysis of the nemipterus virgatus myosin peptide, and the biological propertiesand yield of the immunomodulating peptides; the anti-inflammatory activity of the immunomodulatory peptides prepared by the method is better; the invention realizes the deep processing and comprehensive utilization of the low-value fish, and has important meaning for improving the added value of the low-value fish.