37 results about "Antibody" patented technology

The invention relates to anti-integrin antibodies which are covalently linked to nanoparticles, wherein these nanoparticles were prior loaded with chemotherapeutic/cytotoxic agents. The antibody-chemotherapeutic agent-nanoparticle conjugates according to the invention, especially wherein the antibody is MAb DI17E6 and the cytotoxic agent is doxorubicin show a significant increase of tumor cell toxicity.

A human fusion antibody for preventing and treating senile dementia features that the beta-amyloid peptide and the recognition and bind regions of the fibres generated by it, the connecting peptide of 2-4 amino acids, and the human antibody Fc segment recognized by human immunocells are sequentially contained by its terminals from N to C. The fusion gene for coding the said antibody is also disclosed.

The current invention relates to the development and methods of use of a recombinant agonistic antibody anti-human CD137, and glycosylation variants thereof. These antibodies act as an anti-cancer agents and/or immune modulators that are effective in shrinking solid tumors or other cancerous indications and preventing their recurrence. The types of cancer for which the contemplated antibody is effective in treating also include leukemia and lymphoma. In a preferred imbodiment the recombinant antibodies of the current invention were produced in and purified from the milk of transgenic animals.

The present invention discloses a novel device and methods thereof with utility application in the high-throughput detection, screening and disease management of cervical disease. The “multiwell” device of the present invention consists of a solid support featuring multiple well-separated areas, each accommodating a patient sample, leading to simultaneous evaluation of patient samples. The methods of the present invention comprise conventional cytological staining, cervical Pap staining, and immunochemical staining using antibodies or combination of antibodies which are capable of binding to biomarkers that are overexpressed in cancer including in cervical carcinoma and dysplasia, as compared to normal controls. The device and methods of the present invention can be practiced in either manual or automated mode, and applied to any biological fluid or cell suspension from any biological specimen in view of a variety of cell biology assays, and in view of detection and screening of cervical and other diseases.

Antibodies which specifically bind to components of the DNA synthesome which are altered in malignant cells are disclosed. These antibodies can be used, inter alia, to diagnose, prognoses, and treat malignancy and in assays to screen cells, tissues, and body fluids for the presence of a malignant phenotype. These antibodies can be further used to identify test compounds having the ability to suppress the malignant phenotype in a cell by assaying for the ability to inhibit or block the function of an altered component of the DNA synthesome associated with the malignant phenotype. Further, disclosed herein are methods and kit for minimally invasively detecting the presence of neoplasms and malignant conditions using easily obtainable body fluids, such as blood, plasma, lymph, pleural fluid, spinal fluid, saliva, sputum, urine, and semen, for example, to both detect the presence of cancer as well as assess the stage of the disease and the prognosis of the patient. By detecting the presence of an altered form of a component of the DNA synthesome in body fluid, one can diagnose and prognose malignancy. The disclosed method and kit therefor can be used as a diagnostic biomarker for malignancy as well as a means of monitoring the progress and effectiveness of therapeutics.

The invention discloses a kit for detecting the content of a KAPPA light chain.The kit comprises a reagent R1, a reagent R2 and a KAPPA light chain calibration material, wherein the reagent R1 comprises a first buffer solution, a first electrolyte, a surface active agent, a first stabilizer, a macromolecule accelerant and a first preservative, the reagent R2 comprises a second buffer solution, an anti-human KAPPA light chain antibody, a second electrolyte, a second stabilizer and a second preservative, and the C1 inhibitor calibration material comprises a third buffer solution, a third stabilizer, a third preservative, an antioxidant and a KAPPA light chain antigen.The invention further discloses the application of the kit for detecting the content of the KAPPA light chain and a method for detecting the content of the KAPPA light chain using the kit.By the adoption of the kit and the detection method, the content of the KAPPA light chain can be detected easily and quickly.

An immunological measurement is performed using anti-CTP antibody characterized by recognizing an antigenic determinant included in the polypeptide represented by amino acid numbers 118-132 of SEQ ID NO: 1, and reacting with native Cochlin-tomoprotein (CTP).

The invention relates to the field of biological detection and the field of nanomaterial functional application, and discloses a method for preparing a core-shell SERS structure based on the amplification of a nucleic acid strand by terminal deoxynucleotidyl transferases. The method comprises the following steps of: assembling a starting strand on the surface of a nanomaterial, carrying out the DNA amplification reaction on the surface of the terminal deoxynucleotidyl transferase, transferring deoxynucleotide to the starting primer strand assembled on the surface of the nanomaterial so as to form long single-stranded DNA on the surface of the nanomaterial and then obtain a nanomaterial-long-single-stranded-DNA complex; and covering a metal shell layer outside the nanomaterial-long-single-stranded-DNA complex by the chemical reduction method so as to form the core-shell structure. The core-shell SERS structure has long strand DNA which can be used as a Raman reporter small molecule so as to output efficient and uniform SERS signals to enhance the intensity of Raman signals. Bases are located between the core and the shell, so that the number of ''hot spots'' in the core-shell structure is increased, the adsorption of target molecules or antibodies on the surface of the core-shell structure is affected, the sensitivity of the detection can be greatly improved, and the method canbe applied to the field of biological analysis and detection.

A method of detecting the situation of existence of human immunodeficiency virus in biological samples is provided. Particularly, by detecting the situation of existence of antibodies against human immunodeficiency virus proteins in biological samples, it is judged whether HIV infection exists. Reagent kits used in the method are also provided.

The present invention is directed to multivalent DR5-Binding Molecules that comprise Binding Domain(s) of anti-DR5 antibodies, and particularly Binding Domain(s) of anti-human DR5 antibodies. The DR5-Binding Molecules of the present invention include bivalent and tetravalent molecules having two, three or four DR5-Binding Domains each capable of binding human DR5. In particular, the present invention is directed to multivalent DR5-Binding Molecules that comprise diabodies, and more particularly, diabodies that comprise a covalently bonded complex of two or more polypeptide chains. The invention particularly pertains to such multivalent DR5-Binding Molecules that comprise of the anti-DR5 antibodies DR5 mAb 1 and/or DR5 mAb 2, and/or humanized and chimeric versions of such antibodies.

The invention discloses a traditional Chinese medicine health preservation medicine bag with the effects of invigorating the stomach and promoting the digestion. The traditional Chinese medicine health preservation medicine bag is prepared from the following ingredients in parts by weight: 5 to 10 parts of semen euryales, 10 to 15 parts of coix seeds, 1 to 5 parts of polished round-grained rice, 5 to 10 parts of radix glycyrrhizae preparata, 30 to 40 parts of fructus crataegi, 10 to 15 parts of lemon, 20 to 30 parts of radix pseudostellariae, 5 to 10 parts of red beans, 5 to 10 parts of Chinese yam, 10 to 15 parts of malt and 5 to 10 parts of poria cocos. A traditional Chinese medicine form is used for achieving the effects of promoting the digestion, realizing dehumidification and simultaneously preserving the health on patients; the medicine effect of the traditional Chinese medicine is slow, but the problems can be fundamentally solved from the human body through the long-period conditioning of the traditional Chinese medicine; the human body cannot generate an antibody to the traditional Chinese medicine.

A label that permits rebridging of disulfide linkages in antibodies or proteins. The label has a general formula given by

wherein R₁ is methyl, ethyl or propyl and R₂ is a metal chelator, a fluorophore or a click-chemistry synthon.

The present description relates to an anti-S1008 protein for treating leukemia. More specifically, is disclosed anti-S100A8 antibody that specifically binds to a portion of S100A8 protein and/or a S100A8/S100A9 heterodimer for treating leukemia.

The invention discloses a magnetic MOF@aptamer and a method for high-sensitivity fluorescence detection of food-borne pathogenic bacteria by the same, which mainly comprises the following steps: preparing Fe3O4@ZIF-8 by solvothermal and layer-by-layer self-assembly method, modifying the Fe3O4@ZIF-8 by using the aptamer of target bacteria to prepare Fe3O4@ZIF-8@aptamer, capturing and separating the target bacteria, furthermore, using the FITC fluorescence labeled antibody for identifying the target bacteria, so that the fluorescence resonance energy transfer between the FITC fluorescence molecule and the surface of the Fe3O4@ZIF-8 metal group is realized, and the fluorescence enhancement of the FITC is realized. The research method disclosed by the invention has the advantages of high detection sensitivity, strong specificity, short detection time and the like, and is suitable for rapid detection of food-borne pathogenic bacteria in food samples.

The invention provides a cotton swab detection device for rapidly collecting human immunodeficiency viruses. The device comprises a detection box; an immunochromatography detection strip and a conjugate pad are arranged in the detection box; the conjugate pad is connected with the immunochromatography detection strip in an overlapping manner; a sample clamping assembly and a sampling swab are arranged at the front end of the detection box; the sampling swab is detachably connected to the front end of the detection box through the sample clamping assembly; the sampling swab is of a permeable structure; the rear end of the sampling swab is connected with the conjugate pad in an overlapped mode; the immunochromatography detection strip is sequentially composed of a sample absorption area, a solid-phase labeled antibody area, a detection area, a contrast area and a water absorption area from left to right; and viruses enter the sample absorption area through the sampling swab and the conjugate pad in sequence. According to the cotton swab detection device of the invention, the sampling swab is used for collecting viruses or saliva, the AIDS viruses can be rapidly sampled, the saliva is filtered through the conjugate pad, and the viruses permeate into the immunochromatography detection strip through the conjugate pad, so that the purpose of detecting the AIDS viruses is achieved.

Methods for the preparation of polymeric films which encase and preserve bioactive agents. In particular, the invention is directed to the preparation of oral thin films containing bioactive proteins or viruses. For example, methods and compositions are disclosed for preservation of rotavirus and antibodies in thin dry films.

The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands. Finally, the field of the invention also encompasses multi-omic MSI procedures, where MSI of photocleavable mass-tag probes is combined with other modes of MSI, such as direct label-free MSI of endogenous biomolecules from the biospecimen (e.g., tissue), whereby said biomolecules can be intact or digested (e.g., chemically digested or by enzyme).

The invention relates to a composite antibody extract, a preparation method and applications thereof. The preparation process of the composite antibody extract comprises: preparing a composite antigencontaining facial pathogenic bacteria, carrying out strengthening immunization on chicken, obtaining immunized eggs, separating and purifying the egg yolks of the immunized eggs to obtain a crude extract, and continuously separating and purifying to obtain an extract containing a composite antibody against the facial pathogenic bacteria. According to the present invention, the prepared compositeantibody extract has characteristics of high stability, strong antibody specificity and good safety, and can effectively resist the infestation of facial pathogenic bacteria, effectively resist bacteria and remove acne, and reduce the recurrence of acne.

The invention discloses a preparation method of an electrochemiluminescence sensor for detecting organochlorine pesticides. The preparation method is characterized in that firstly a novel two-dimensional nanocomposite-titanium dioxide/molybdenum disulfide composite, namely an iron and manganese codoped titanium dioxide nanodiamond and molybdenum disulfide in-situ composite two-dimensional nanocomposite FeMn-TiO2/MoS2, is prepared, organochlorine pesticide antibodies are loaded by utilizing the good biocompatibility and large specific surface area of the material, during detection, iron and manganese codoped titanium dioxide can catalyze hydrogen peroxide to generate O2 in situ and carry out electrochemical reaction with K2S2O8 in a base solution to generate electrochemiluminescence signals, and the impacts of specific quantitative combination of the antibodies and antigens on the electron transport capacity are utilized, so that the current intensity is reduced, thus reducing the luminescence intensity and finally achieving construction of the electrochemiluminescence sensor for detecting the organochlorine pesticides by adopting an unmarked electrochemiluminescence method.

The invention discloses a chemiluminescence immune detection kit of an anti-trophoblast cell membrane antibody and a preparation method of the chemiluminescence immune detection kit. The chemiluminescence immune detection kit of the anti-trophoblast cell membrane antibody comprises carboxylated magnetic micro particles coated with anti-trophoblast cell membrane antibody recombinant protein and an immunoglobulin-marked chemiluminescence marker. By the chemiluminescence immune detection kit of the anti-trophoblast cell membrane antibody, the anti-trophoblast cell membrane antibody is detected by a full-automatic chemiluminescence immunity analyzer as a detection tool. Through an experiment, the detection sensitivity of the chemiluminescence immune detection kit of the anti-trophoblast cell membrane antibody reaches 1U/L; compared with a traditional detection method of the anti-trophoblast cell membrane antibody, the sensitivity is improved by 10 times at least; and the chemiluminescence immune detection kit of the anti-trophoblast cell membrane antibody is relatively high in detection accuracy.

The invention is based on the development of several sensitive and simple methods for detecting erythropoietin and the erythropoietin receptor and associated antibodies. The methods and reagents are useful for differential diagnosis in Epo and EpoR related clinical problems. Methods and kits for simultaneous measurement of erythropoietin and erythropoietin receptor in a biological sample are described.

The present invention provides anti-plazomicin antibodies and methods of use thereof, e.g., in determining levels of plazomicin in samples from human patients undergoing treatment with plazomicin. Such antibodies are useful, for example, in monitoring plazomicin levels during treatment for an infectious disease.