Kit and method for detecting content of KAPPA light chain and application of kit
A kit and light chain technology, applied in the field of kits for detecting KAPPA light chain content, can solve the problems of inability to make a clear diagnosis, inaccurate quantification, and long detection time in atypical patients, and achieve a high degree of automation and high detection efficiency. , the effect of improving the detection efficiency
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Embodiment 1
[0058] The kit for detecting the content of KAPPA light chain comprises: reagent R1, reagent R2 and KAPPA light chain calibrator.
[0059] Wherein, reagent R1 comprises the following components:
[0060]
[0061] Reagent R2 includes the following components:
[0062]
[0063]
[0064] KAPPA Light Chain Calibrators include the following components:
[0065]
[0066] According to the required concentration of the calibrator, add the corresponding amount of KAPPA light chain antigen to the mixture of other reagents above, and mix to obtain the KAPPA light chain calibrator. In this example, 5 different concentrations of KAPPA light chain calibrator were prepared, and the concentrations of the KAPPA light chain antigen were: 0mg / dL, 200mg / dL, 400mg / dL, 800mg / dL and 1200mg / dL. The KAPPA light chain calibrator was sterilized by filtration with a 0.22 μm filter membrane, and stored at 2-8°C.
[0067] Using the above kit, the samples to be tested were detected and
Embodiment 2
[0069] The kit for detecting the content of KAPPA light chain comprises: reagent R1, reagent R2 and KAPPA light chain calibrator.
[0070] Wherein, reagent R1 comprises the following components:
[0071]
[0072] Reagent R2 includes the following components:
[0073]
[0074]
[0075] KAPPA Light Chain Calibrators include the following components:
[0076]
[0077] According to the required concentration of the calibrator, add the corresponding amount of KAPPA light chain antigen to the mixture of other reagents above, and mix to obtain the KAPPA light chain calibrator. In this example, 5 different concentrations of KAPPA light chain calibrator were prepared, and the concentrations of the KAPPA light chain antigen were: 0mg / dL, 200mg / dL, 400mg / dL, 800mg / dL and 1200mg / dL. The KAPPA light chain calibrator was sterilized by filtration with a 0.22 μm filter membrane, and stored at 2-8°C.
[0078] Using the above kit, detection and analysis were carried out by
Embodiment 3
[0080] The kit for detecting the content of KAPPA light chain comprises: reagent R1, reagent R2 and KAPPA light chain calibrator.
[0081] Wherein, reagent R1 comprises the following components:
[0082]
[0083] Reagent R2 includes the following components:
[0084]
[0085] KAPPA Light Chain Calibrators include the following components:
[0086]
[0087]
[0088] In this embodiment, a high-concentration single-point calibrator is selected, so the concentration of the KAPPA light chain antigen is 1200 mg / dL. The KAPPA light chain calibrator was sterilized by filtration with a 0.22 μm filter membrane, and stored at 2-8°C. When in use, dilute with normal saline to form 5 different concentrations of KAPPA light chain calibrator, the concentrations of the KAPPA light chain antigen are: 0mg / dL, 200mg / dL, 400mg / dL, 800mg / dL and 1200mg / dL.
[0089] Using the above kit, detection and analysis were carried out by Hitachi 7080 automatic biochemical analyzer. Th
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