335 results about "Biotechnology" patented technology

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

The invention discloses a plant antifreezer and its preparation method. The plant antifreezer is preparegggggd from the following components of: by weight, 0.5-0.97% of glucose, 0.5-0.97% of cane sugar, 0.2-0.5% of potassium dihydrogen phosphate, 0.18-0.21% of novel monomolecular lipid film, 0.06-0.17% of paclobutrazol, 0.2-0.33% of amino acid, 0.02-0.05% of 6-benzylamino-purine, 0.01-0.05% of rapin, 46-48.4% of glycerin and 48.6-51% of water. The plant antifreezer provided by the invention is nontoxic and harmless, doesn't pollute the environment, and can be directly sprinkled on plant leaf surfaces, flowers and flower bus. The preparation method is simple and requires low cost. Effective antifreezing period can reach 15-20 days. By the adoption of the plant antifreezer, lower temperature freeze injury resistance of plants can be enhanced and the freeze injury can be rapidly mitigated after freeze injury. The plant antifreezer provided by the invention can be used to form a protection film on the surface of a plant and protect the plant from cold, and has an effect of preventing diseases.

A fermentation process for the production of ethanol from natural sources, such as corn, comprising introducing a fermentable sugar and an inoculant, and a bacteriophage cocktail into a fermentation system and introducing a cocktail comprising one or more lytic bacteriophage is added to one or more of the fermentable sugar, the inoculant, or the fermentation system. Bacteriophages that infect and lyse the bacteria that contaminate fermentable sugars are selected. The bacteriophage cocktail is added in an amount effective to substantially prevent growth of these bacteria.

The invention discloses electronic tobacco juice with baking aroma. A preparation method for the electronic tobacco juice with the baking aroma comprises the following steps of mixing tobacco powder, tobacco shreds or tobacco ash and solvent according to mass-to-volume ratio of 1:5-10; distilling the mixture for 1-4 hours at the temperature of 120-200 DEG C; and feeding inert gas to blow distilled matters in a distilling process until an absorbing liquid is formed so as to obtain the electronic tobacco juice. The tobacco powder, the tobacco shreds or the tobacco ash serve as raw materials; matters with the baking aroma in the tobacco powder, the tobacco shreds or the tobacco ash are extracted and separated from the tobacco powder, the tobacco shreds or the tobacco ash by using a distilling device at certain temperature; the aroma matters of the aroma are quite similar to ingredients of materials of baking aroma generated in the traditional cigarette burning process; after the electronic tobacco juice with the baking aroma is atomized by an atomizer, the feeling of a smoker smoking the electronic tobacco juice is consistent with the feeling of the smoker smoking the traditional cigarettes; and the electronic tobacco juice has the baking aroma generated in the cigarette burning process.

The invention provides a chicken meatball containing kudzu-vine root powder; the chicken meatball is composed of the following raw materials by weight parts: 50-70 parts of chicken breast, 20-30 parts of chicken skin, 2-4 parts of kudzu-vine root powder, 40-50 parts of ice water, 2-3 parts of salt, 1.5-2 parts of white granulated sugar, 3-4 parts of water-retaining agents, 8-10 parts of thickening agents and 2-3 parts of flavor enhancer; in the product, the kudzu-vine root powder which has multiple functions and high food therapy value is added, so as to improve the level of the chicken meat products, enrich the nutrition thereof and improve the health-care function.

The invention discloses primers and a method for purity identification of hybrid seeds of Chinese pumpkin 'Guangmi NO.1'. The primers are VRISSRPMK8-F:5'-TAATTAAGGCGTGAAATTGG-3' and VRISSRPMK8-R:5'-GAAAAGAAACATAAACAGGGC-3'. The identification method is as follows: 1) extracting a genome DNA (deoxyribonucleic acid) of a pumpkin seedling; 2) carrying out PCR (polymerase chain reaction) amplification by taking the genome DNA of the pumpkin as a template and using the SSR primers VRISSRPMK8; 3) carrying out gel electrophoresis on the amplified product; and 4) analyzing the electrophoresis result, wherein only the single plant simultaneously having parent specific bands is a real hybrid, and the plant lacking of any one band is marked as false hybrid. The primers and the method can be used for effectively distinguishing the hybrid seed of the 'Guangmi NO.1' from the female parent and the male parent, and can identify the purity of the hybrid seeds rapidly and accurately. The method has the advantages of being fast and accurate, low in cost and simple in operation, and the like.

The invention relates to new application of melatonin to improving saline-alkaline stress resistance of plant seeds. A product taking the melatonin as an effective ingredient is applied to the plant seeds under saline-alkaline stress, and the melatonin is a compound with the high lipophicity and hydrophilcity and can get in and out of plant cells freely, so that the melatonin can play a role in plant tissues. The melatonin can be applied to the plant seeds under the saline-alkaline stress conveniently, can be used for promoting the germination rate and the germination potential and reducing the salt damage rate of the plant seeds under the saline-alkaline stress, and can be used for promoting the seed germination and seed vigor, so that the purposes of keeping a full stand of seedlings, strengthening the seedlings, and preventing the reduction of yield and quality, caused by the saline-alkaline stress, can be achieved.

The invention belongs to the technical field of cosmetics, and concretely relates to a whitening moisture-retention essence and a preparation method thereof. The whitening-retention essence comprises the following raw materials in parts by weight: 0.2-0.5 parts of a thickening agent, 3-12 parts of polyol, 15-35 parts of a traditional Chinese medicine extract product, 1-2 parts of honey, 0.3-2 parts of pearl powder, 0.1-0.3 parts of essence and 40-70 parts of water. The preparation raw materials of a traditional Chinese medicine extract product comprise eucommia, seeds of Job's tears and blueberry fruit. The whitening moisture-retention essence has the advantages of good permeability and easy absorption, and has the efficacy for whitening and keeping moisture, restoring skin wrinkles, improving dark skin, enhancing skin luster and delaying skin aging.

The invention discloses denitrifying phosphorus removal bacteria bacillus cereus H-hrb01, and belongs to the technical fields of biological engineering and environmental engineering, wherein bacillus cereus H-hrb01 belongs to bacillus genus by identification and is conserved in the general microbiology centre of the China committee for culture collection of microorganisms, the conservation date is March 27th 2012, and the conservation number is CGMCC NO.5939; bacillus cereus H-hrb01 can take various organic matters as carbon sources, has good denitrifying and phosphorus-removing efficiency, and can be used for sewage biological denitrification and dephosphorization process; bacillus cereus H-hrb01 is activated and cultivated, and fed into artificial wastewater to mix and then introduced into an SBR (styrene butadiene rubber) biofortification device, and each effluent index of the reactor is superior to that of a contrast system which is not fed with the bacterial strain. The removal rates of the COD (chemical oxygen demand), ammonia nitrogen and dissoluble phosphorus in the sewage are respectively up to 93.90%, 97.70% and 96.25%.

The invention discloses an extraction method of ginger essential oil. The method comprises the following steps: preparing ginger powder: slicing fresh ginger, soaking the sliced ginger in a 0.5-0.9wt% starch solution for 10-15min, drying and grinding the sliced ginger, and filtering obtained powder through a sieve of 60-80 meshes; preparing enzymatic hydrolysate: adding a de-ionized liquid 1-ethyl-3-methyl imidazole acetate ([Emim]OAc) to a disodium hydrogen phosphate-citrate buffer solution to obtain an ionic liquid system; adding the ginger powder to the ionic liquid system, controlling a material-to-liquid ratio at (1: 10)g/ml-(1: 20)g/mL, adding cellulase which is 2000-4000U/g in enzyme activity ratio and enzymatically hydrolyzing for 3-5h at 40-50 DEG C; and 3) essential oil extraction: and implementing enzyme deactivation to the system in a water bath after the reaction, ultrasonically extracting for 5-10min, conducting microwave treatment, filtering through gauze, centrifuging, standing and taking the de-ionized liquid on lower layer, spin-steaming, collecting oily matters having intense fragrance and drying the oily matters through anhydrous sodium sulfate to obtain the ginger essential oil.

The invention provides a beverage for beautifying features and a preparation method of the beverage, and relates to the technical field of foods. The raw materials of the beverage for beautifying features mainly comprise the following components in parts by weight of 0.5-10 parts of procellariidae, 0.5-10 parts of prunus persica, and 0.5-10 parts of gleditsia sinensis fruits. The procellariidae has the effect of regulating the growth of human body collagen, and the prunus persica and the procellariidae are interacted, so that the efficacy of resisting ageing can be achieved to the maximum extent. The gleditsia sinensis fruits can internally adjust the heart, the liver, the spleen and the kidney, so that the purposes of quickly moistening skins and maintaining beauty are realized. The invention further provides the preparation method of the beverage for beautifying features. The method is simple and easy to operate, and nutrient components of the raw materials can be reserved to the greater extent, so that the raw materials are mutually matched to achieve good and fast efficacy of beautifying the features.

The invention provides a selenium-enriched mushroom grass culture medium which is prepared by taking selenium-enriched mushroom grass, wood chips, wheat bran, saccharose, gypsum, monopotassium phosphate and magnesium sulfate as the main raw materials. The invention further provides a method for producing shiitake mushrooms through the selenium-enriched mushroom grass culture medium. Accordingly, a traditional shiitake mushroom producing method is changed, a selenium-enriched mushroom grass technology is utilized in shiitake mushroom stick production, therefore, the producing method of selenium-enriched products is changed, the nutritional value of the shiitake mushrooms is increased, the production efficiency is improved, the shiitake mushroom yield and biotransformation rate are high, and meanwhile the production cost is greatly lowered; in addition, the producing method is simple in technology, the produced shiitake mushrooms are high in content of selenium-enriched elements, the product is free of pollution, and the food safety problem is solved from the source.

According to the invention, there is provided a novel soybean variety designated XB49Q07. This invention thus relates to the seeds of soybean variety XB49Q07, to the plants of soybean XB49Q07 to plant parts of soybean variety XB49Q07 and to methods for producing a soybean plant produced by crossing plants of the soybean variety XB49Q07 with another soybean plant, using XB49Q07 as either the male or the female parent.

Methods are provided for controlling metabolic flux rate in a cell-free system comprising a complex set of enzymes, to produce a desired product of a pathway of interest. In the methods of the invention, measurements of metabolic performance parameters are taken by continuous monitoring or intermittent monitoring. Based on the metabolic performance parameters, the system is modified by one or more steps comprising: (i) altering enzyme levels in the cell-free system; (ii) altering feed rate of a substrate that controls redox flux or carbon flux to the cell-free system; (iii) altering O₂ addition to the cell-free system; (iv) controlling efficiency of electron transport system by altering leakage across a membrane; wherein enzymes present in the pathway of interest catalyze production of a desired product.

The present invention relates to an engineered biological material comprising or enriched for tissue of intervertebral disc; tissue derived from an engineered biological material; constructs comprising one or more tissues from an engineered biological material; methods for producing the engineered biological materials and constructs; and methods of using the engineered biological materials and constructs.

The invention discloses culture media for edible mycorrhizal fungi and symbiotic seedlingsand and a synchronous culture method by utilizing the culture media. The culture media for the edible mycorrhizal fungi and the symbiotic seedlings comprise an edible mycorrhizal fungus strain mycelium culture medium and an edible mycorrhizal fungus symbiosis seedling synthesis culture medium, and the ediblemycorrhizal fungus strain mycelium culture medium includes a solid culture medium used for expanding reproduction of the mycelium and a liquid culture medium used for culturing mycelium which is usedfor inoculation of the mycorrhizal symbiotic seedlings. The synchronous culture method for implementing edible mycorrhizal fungus symbiotic seedling synthesis by utilizing the above culture media comprises the following steps: (1) a cultivation method for the edible mycorrhizal fungus strain mycelium, (2) a cultivation method for the edible mycorrhizal fungus symbiotic seedlings. According to theinvention, cultivation of the edible mycorrhizal fungus mycelium is divided into two stages including solid culture and liquid culture, liquid fungus strains are adopted for inoculation, and meanwhilethe edible mycorrhizal fungus mycelium and symbiotic plants in the mycorrhizal seedling synthesis culture medium grow together, so that the infection ability of the mycelium is increased by 30% or more, the success rate of the inoculation is increased by 20%-30%, and the survival rates of symbiotic plant seedlings and the mycorrhizal fungus mycelium are both increased by 20% or more.

The invention provides a quick-frozen glue pudding made of hydroxypropyl sticky rice starch. The quick-frozen glue pudding is made of sticky rice flour, hydroxypropyl sticky rice starch, compound modified starch, monostearin, quick-frozen grease and water, wherein the weight of the hydroxypropyl sticky rice starch is 0.6%-1.4% of the weight of the sticky rice flour; the weight of the compound modified starch is 0.6%-1.4% of the weight of the sticky rice flour; the weight of the monostearin is 0.18%-0.26% of the weight of the sticky rice flour; and the weight of the quick-frozen grease is 1.0%-2.0% of the weight of the sticky rice flour. The excellent freeze-thaw stability, boiling fastness and paste stability of the hydroxypropyl sticky rice starch are utilized by the quick-frozen glue pudding to eliminate the phenomena of cracking, soup-confusing and the like of the quick-frozen glue pudding on the market. The quick-frozen glue pudding made of the hydroxypropyl sticky rice starch has the advantages of good taste, high water-retaining property, high freeze-thaw stability and excellent cohesiveness.

According to the invention, there is provided a novel soybean variety designated XB39N05. This invention thus relates to the seeds of soybean variety XB39N05, to the plants of soybean XB39N05 to plant parts of soybean variety XB39N05 and to methods for producing a soybean plant produced by crossing plants of the soybean variety XB39N05 with another soybean plant, using XB39N05 as either the male or the female parent.

The invention relates to a medlar plant energy beverage and a production method thereof. The medlar plant energy beverage is prepared from a plurality of herbs, such as medlar, liquorice, red dates, semen cassiae, medlar nectar, chrysanthemum and folium mori in a balanced manner. According to the medlar plant energy beverage and the production method thereof, a common functional beverage addition method is changed, and caffeine, preservatives and artificial pigments are not added; meanwhile, the medlar plant energy beverage breaks through a traditional administration mode, can be drunk at any time and has the functions of enhancing immunity of a human body and relieving asthenopia and physical fatigue. According to the medlar plant energy beverage prepared by adopting the production method, the shelf life is prolonged and the original flavors are furthest enhanced; the medlar plant energy beverage tastes sour and sweet, is uniform and stable in quality and has the characteristics of enhancing immunity of the human body and relieving asthenopia and physical fatigue.

The invention provides a simple method for extracting natural taurine from abalone viscera. The invention is characterized by comprising the following steps: raw material pretreatment: adding 2 times of weight-to-volume ratio of water into abalone viscera, boiling at high temperature, and collecting the soup; activated carbon treatment: passing the soup through an activated carbon column at room temperature to obtain an effluent liquid; removing impurities with ethanol, and centrifuging to obtain a precipitate and a supernatant; concentrating the supernatant to obtain a concentrated solution; adding anhydrous ethanol into the concentrated solution, standing at 4 DEG C, centrifuging to obtain a precipitate, dissolving the precipitate in hot water, adding activated carbon, stirring, and carrying out vacuum filtration to remove the activated carbon, thereby obtaining a filtrate; crystallizing to obtain a taurine crystal; and carrying out recrystallization one or more times as required. The invention has the advantages of simple technique, low extraction cost, high extraction rate, energy saving and environmental protection. The method provided by the invention can effectively utilize abalone processing byproducts and enhance the utilization ratio of ocean resources. The ethanol used in the method can be recycled, thereby being beneficial to industrialized production.

The invention belongs to the technical field of microbes, and particularly relates to a high-yield production method of lovastatin and monascus used by the production method. The production method comprises the following steps: strain mutagenesis and fermentation by using the mutagenesis strain. Compared with the conventional monascus production strain and production method, the mutagenesis strain and method for producing lovastatin by using the mutagenesis strain have the following advantages: the lovastatin yield can reach 1650 mg/L above, the lovastatin productivity is higher, and the lovastatin quality is improved.

The invention discloses an ovum and embryo treating fluid and a preparation method thereof. The ovum and embryo treating fluid contains composition and a sterilizing agent used for carrying out treatment and culture on an ovum and an oosperm, and an indicator can be selectively added. Indicators of the ovum and embryo treating fluid meet standards of an in vitro culture solution, the ovum and embryo treating fluid is safe to use, and whether the culture solution goes bad or not can be easily judged, and a strong guarantee is provided for an assisted reproductive technology.

The invention provides a traditional Chinese medicine microbial ecological agent for preventing and treating infectious bursal disease of chicken and a preparation method thereof. The preparation method comprises the following steps: adding water to radix isatidis, honeysuckle, forsythia, folium isatidis, humulus scandens, radix scutellariae, dangshen, ageratum, plantain, astragalus membranaceus, angelica sinensis and liquorice, boiling by using high heat, preserving heat for 30-60 minutes by using slow fire, cooling, filtering, collecting filtrate, adding white sugar, boiling, cooling to a normal temperature, then inoculating lactobacillus, zygosaccharomyces bailii and acetobacter xylinum, and fermenting for 3-7 days so as to obtain the microbial ecological agent. After the microbial ecological agent is diluted proportionally, the diluted microbial ecological agent is directly drunk by chicken. The traditional Chinese medicine microbial ecological agent provided by the invention has the effects of resisting viruses, inducing interferon, increasing the quantity of effective microbial communities in intestinal tracts, boosting immunity and improving disease resistance of chickens without toxic or side effects and the like; the preparation method is simple, convenient to use and high in viable bacteria content and has no residues. The traditional Chinese medicine microbial ecological agent provided by the invention has a certain curative effect on infectious bronchitis and henpox and relatively good market prospect.