32 results about "Centrifugation" patented technology

The invention discloses a method for comprehensively extracting fucoxanthin and brown alga polyphenol from gulfweed, which comprises the following steps: carrying out repeated freeze-thawing and centrifugation on fresh gulfweed or frozen reserved gulfweed to control the water content of brown alga; adding an organic reagent into the seaweed, leaching in a dark place for 1-24 hours, carrying out vacuum filtration to obtain a leaching solution, repeating the leaching operation until the leaching solution is colorless, merging the leaching solutions, concentrating the leaching solution until abundant precipitate appears in the solution, and filtering to remove the precipitate and obtain the filtrate; crystallizing the filtrate at low temperature, and filtering to obtain fucoxanthin; and adding a fat-soluble solvent into the filtrate, carrying out repeated liquid-liquid extraction until the fat-soluble solvent layer is colorless, merging the fat-soluble solvent layers of multiple extraction processes, and carrying out low-temperature concentration until a greenish yellow solid precipitates in the solution, thereby obtaining the brown alga polyphenol extract. The method has the advantages of high raw material utilization ratio and high extraction efficiency, and is simple to operate.

The invention discloses a rapid and high efficiency chlorogenic acid and galuteolin in honeysuckle flower extraction method and a HPLC quantitative analysis method, and which comprise the following steps: superhigh pressure extraction and quantitative analysis. Superhigh pressure extraction comprises the steps as follows: firstly crushing dried honeysuckle flower and filtering by a sieve with 60-80 meshes, adding water or ethanol, methanol and n butanol organic solvent into the honeysuckle flower powder according to 1:10-1:100 solid liquid ratio, mixing and sealing, immersing at normal temperature, placing the mixture into a high pressure container, leaching with pressure at 20-60 DEG C. for 1-5 min, relieving pressure, carrying out centrifugation of extract product and obtaining the honeysuckle flower extract. Quantitative analysis: after centrifugation, diluting 2ml of the obtained supernatant to 10 ml with distilled water in a volumetric flask, refrigerating for standby. The chlorogenic acid and galuteolin in honeysuckle flower are extracted by a superhigh pressure technology and are carried out by a content determination, and the invention has the characteristics of high recovery rate, short extraction time, small solvent amount, simple operation, accuracy, good repeatability, no pollution, safety, low energy consumption, etc.

The invention discloses a method for refining a high-temperature wood hydrolyzate. The method comprises the following steps: performing centrifugation on a raw material hydrolyzate; removing the water-soluble macromolecular lignin by a flocculation method; obtaining the hydrolyzate after flocculation, adsorbing the water-soluble small molecule lignin with the resin, and obtaining a purified xylosesolution; removing residual lignin of the purified xylose solution by a filtering device to obtain refined xylose liquid and prepare xylose; and eluting the resin-adsorbed lignin with an eluting solvent, and recovering the eluting solvent and lignin. The recovered ethanol of the invention can be recycled and reused; an integrated vortex reactor is also used to effectively improve and increase theflocculation effect, and the ion exchange resin as an adsorbent has a good adsorption effect on the charged lignin. The hydrolyzate improves the membrane separation effect of a filter membrane and maintains a large flux of the membrane.

The invention discloses a method for preparing a silver micron hollow frame structural material. The method comprises the following steps of firstly, preparing a AgNO3 aqueous solution at the room temperature, then, adding the appropriate quantity of a structure-directing agent HF and a reducing agent FeSO4 into the AgNO3 aqueous solution to form a mixed solution and furthermore, enabling the mixed solution mentioned above to stand in water bath of a certain temperature, keeping for a period of reaction time, and obtaining the silver micron hollow frame structural material after carrying out centrifugation, washing and drying on a product. The method for preparing the silver micron hollow frame structural material is simple, and with the method, large-scale production of the silver micronhollow frame structural material is realized.

The invention discloses a method for rapid determination of isothiazolinone compounds in a water-based adhesive for cigarettes. The method comprises the following steps: adding an extraction agent into the water-based adhesive for cigarettes, wherein a volume mass ratio of the extraction agent to a water-based adhesive sample is 5-40 mL: 0.3 g; then carrying out ultrasonic extraction for 5 to 45 min so as to obtain extract liquor; subjecting the obtained extract liquor to centrifugation, taking supernatant and introducing nitrogen into the supernatant so as to obtain concentrated supernatant; allowing the concentrated supernatant to pass through a filter membrane so as to obtain a to-be-detected solution; and carrying out quantitative analysis on the isothiazolinone compounds in the to-be-detected solution by using ultrahigh performance liquid chromatography-diode array. The method provided by the invention can carry out simultaneous analysis and detection on at least three isothiazolinone compounds in the water-based adhesive for cigarettes; the method is simple in treatment and fast in analysis speed; detection results are good in repeatability and high in sensitivity; and the method is applicable to determination of three isothiazolinone compounds in the water-based adhesive for cigarettes or in other complex matrixes.

The invention provides an extraction method for phellinus vaninii polysaccharide. The method comprises the following steps: crushing a phellinus vaninii fruiting body to 1-5 mesh, performing soaking in physiological saline for 8-24 h, performing centrifugation, and collecting residues; boiling the residues of the phellinus vaninii fruiting body in boiling water at 110-130 DEG C for 20-40 min, performing centrifugation, and collecting residues; adding water into the residues, performing freezing for 24-36 h, and performing unfreezing; adding water into the unfrozen mixture to obtain a mixed liquid, adding a complex enzyme into the mixed liquid, performing enzymatic hydrolysis at 50-60 DEG C for 2-4 h, and performing centrifugal separation to obtain a supernatant liquid; and finally, performing adsorption on the supernatant liquid by using resin, performing dialysis, performing concentration, and performing freeze drying to obtain the phellinus vaninii polysaccharide. According to the extraction method for the phellinus vaninii polysaccharide provided by the invention, the extracted phellinus vaninii polysaccharide has obvious activity of inhibiting tumor cell proliferation, has goodbiocompatibility compared with a polysaccharide extracted and purified by a chemical method, and does not bring an inflammatory reaction; and the phellinus vaninii polysaccharide provided by the invention can be used in anti-tumor drugs or health-care products.

The invention discloses a resurrection factor purification and extraction process for improving injection-type autologous fat survival rate. The process includes the steps: (a) collecting whole blood samples; (b) separating the whole blood samples; (c) fermenting the whole blood samples; (d) freezing fermentative supernatant liquid; (e) enabling whole blood sample cells to resuscitate; (f) performing purification and extraction for resurrection factors: adding liquid after standing into a centrifuging tube, performing secondary centrifugation for the liquid in a centrifugal machine, standing in the constant temperature environments after centrifugation, removing blood plasma on the upper layer of the centrifuging tube, adding new blood samples, and enabling sediment blood platelets to re-suspend to obtain the resurrection factors. According to the process, PRP (platelet-rich plasma) of autologous fat injection can be effectively extracted, extracting efficiency of the PRP from the cells is greatly improved, activities after autologous fat injection can be ensured, autologous fat injection operations are safer and more reliable, and the process is worthy of popularization.

The invention relates to a synthetic method for bifenazate. The method comprises the following specific synthetic sections: step 1, performing nitration: mixing a 4-hydroxybiphenyl solution and a toluene solution under stirring for a reaction, and adding a HNO3 solution dropwise for a reaction to obtain a nitration reaction solution; step 2, performing methylation: mixing the nitration reaction solution and anhydrous sodium carbonate powder for a reaction, and adding a dimethyl carbonate solution dropwise for a reaction to obtain a methylation reaction solution; step 3, performing hydrogenation: throwing the methylation reaction solution, hydrogen gas, and Raney nickel into a reaction kettle for a reaction to obtain a hydrogenation reaction liquid; step 4, performing hydrazination: performing a primary hydrazination reaction, performing a secondary hydrazination reaction, performing filter pressing, performing a tertiary hydrazination reaction, and performing secondary filter pressingto obtain a condensation reaction liquid; step 5, performing condensation: mixing a third hydrazine compound, an ethyl acetate solution and an isopropyl chloroformate solution for a reaction to obtaina bifenazate mixed liquid; and step 6, performing purification: performing desolvation, performing crystallization, performing centrifugation, performing washing, performing secondary centrifugation,and performing drying to obtain the finished bifenazate. The method provided by the invention has the effect of improving purity of the bifenazate product.

The invention discloses a porous carbon material prepared by using self-modification of pseudomonas putida and a preparation method and application thereof. Self accumulation PHA of the pseudomonas putida (Pseudomonas putida KT2440, preservation serial number of ATCC No.47054) is regulated and controlled by changing ingredients of a culture medium, and carbonization is carried out by directly adopting thallus collected by centrifugation to prepare the graded porous carbon material without any excitation steps. The germ self-modification derived porous carbon material has a large number of mesopore structures. The porous carbon material is used as an electrode material of a super capacitor, when electric current density is 0.5 A/g, the specific volume of the porous carbon material reaches 298 F/g; and when the electric current density increases by 20 A/g, the specific volume of the porous carbon material maintains by 234 F/g, and good electric capacity and excellent rate performance aredisplayed. The preparation method has the advantages of novelty, simple operation, low manufacturing cost and the like, the prepared material has the characteristics of graded bore diameter, large specific surface area, good electrical conductivity and excellent electrochemical performance, and is an electrode material for the ideal super capacitor or batteries.

A preparing method of a Ag3PO4@Ag/carbon sphere ternary heterojunction composite material capable of selectively removing cationic dye is disclosed. The method includes subjecting a glucose solution having a mole concentration of 0.5 mol/L to a hydrothermal reaction in a hydrothermal reaction kettle at 180 DEG C for 5-8 h to obtain carbon spheres having an average diameter of 0.3-0.7 [mu]m; addingdropwise ammonia water having a concentration of 10-25 wt% into a AgNO3 solution having a concentration of 0.05-0.2 mol/L to prepare a transparent Tollens' reagent; adding the carbon spheres into theTollens' reagent with the mass ratio of the carbon spheres to the AgNO3 being 0.01-0.1:1 and stirring the mixture for 2-10 h; adding dropwise a Na2HPO4 solution into the mixture with the mole ratio of Ag<+> to PO<4><3-> being 3:1-10; reacting the mixture for 1-3 h; then performing centrifugation, filtration and washing; and drying a product in a vacuum drying oven at 50 DEG C to obtain the composite material. The composite material can rapidly and efficiently remove cationic dye and the synthesized composite material has good stability.

The invention discloses a synthesis method for a porous ZnCo2O4 nanosheet and a pseudo-cubic micro-nano structure. The synthesis method comprises the following steps: (1) weighing CTAB (CetyltrimethylAmmonium Bromide), and dissolving in the mixed solution of deionized water and ethylene glycol at a constant temperature to obtain settled solution; (2) weighing Zn(NO3)2.6H2O, Co(NO3)2.6H2O and ureaat the molar ratio of 1:2:15, dissolving in the solution obtained in (1), and fully stirring; (3) transferring the solution obtained in (2) to a polytetrafluoroethylene lining stainless steel reaction kettle, sealing, and reacting for 3-20h at the temperature of 100-180 DEG C; (4) after reaction is finished, naturally cooling to room temperature, carrying out centrifugation, respectively cleaningwith the deionized water and absolute ethyl alcohol for three times, and drying to obtain a Zn-Co precursor. According to the synthesis method, through the simple regulation and control of reaction parameters, the micro-nano structure of ZnCo2O4 of different morphologies can be obtained through synthesis.

The invention discloses a method for treating sewage through a sewage treatment device. The sewage treatment device comprises a treatment device body and a water storage reaction cavity formed in thetreatment device body. A water level control mechanism is arranged in the water storage reaction cavity, a conversion cavity is formed below the water level control mechanism, a conversion mechanism is arranged in the conversion cavity and comprises a centrifugal bucket, and the blocked centrifugal bucket can be replaced by the conversion mechanism. The water sewage treatment device is simple in mechanism and easy and convenient to use, and sewage is treated efficiently and conveniently so that the emission standard can be met. According to the sewage treatment device, the amount of a disinfector and a flocculating agent is controlled through the water inflow of sewage to be treated, and the situation that due to the fact that the amount of the disinfector and the flocculating agent is insufficient, the purifying effect is not ideal, and due to the fact that the amount of the disinfector and the flocculating agent is too large, environmental damage and resource waste are caused is avoided; the adopted centrifugal bucket can be replaced, and it is avoided that the centrifugation effect is reduced after repeated use.

The invention belongs to the technical field of organic chemistry, in particular relating to a purification method of vitamins, comprising the following steps: resolving the crude vitamin K3 in an organic solvent, such as benzene, ethanol and carbon tetrachloride and the like at the temperature of 70-90 DEG C by adopting a recrystallization technical process; adding activated carbon, stirring and resolving for 3-5 hours, filtering, concentrating the filter, and evaporating excessive organic solvent; stirring the residual mixed liquor at the condition of 0-5 DEG C; and separating out the crystallization; placing the solid-liquid mixture in a centrifugal machine for centrifugation to reach separation of the solid and the liquid; and drying the solid to obtain more than 96% of the pure product vitamin K3. The organic solvent is concentrated and recovered to be recycled. The purification method of the invention has the advantages of high purity, low cost and basic nonexistence of three wastes and the like, and is beneficial for industrial implementation.

The invention relates to a preparation method of medicinal active carbon. The dried bagasse is used as raw materials, and is crushed; then, the materials are added into a potassium carbonate solution to be soaked; the weight ratio of the bagasse to the potassium carbonate solution is 1:(4 to 7); the soaked bagasse is subjected to centrifugation dewatering; then, air isolation is performed for carbonization treatment; carbides are obtained; the carbides are subjected to acid washing by hydrochloric acid, and are then washed by water to a neutral state; finally, drying and sieving are performed. The medicinal active carbon has the advantages that the decoloring speed is high; the adsorption capability is high; the medical property of the medicine per se is not influenced. The bagasse is used as the raw materials; the resource waste and the environment pollution are avoided; the ecological environment protection is facilitated; in addition, the bagasse per se is a safe and nontoxic reproducible substance material; the product added value is high; good social and economic benefits can be generated.

The invention discloses a process for improving yield of carbohydrazide. Dimethyl carbonate and hydrazine hydrate are used as raw materials, methanol is used as a solvent, a carbohydrazide solution is prepared, recrystallization is carried out, and the method concretely comprises the following steps: (1) reaction with insulation, (2) reduced pressure distillation, (3) crystallization, (4) centrifugation, and (5) drying. The method is advantageous in that the content of active component carbohydrazide reaches 98% or above, and the content of free hydrazine reduces to 400ppm or below; in the preparation process, a frequency conversion apparatus is used for separately controlling the temperature of the reaction with insulation, the temperature of reduced pressure distillation, and crystallization temperature; the reaction time is reduced, compared with traditional constant rotational speed process, the yield of the product is increased by 20% or above, at the same time, the content of free hydrazine is obviously reduced, and during crystallization, the rotating speed is low, the growth of crystal grains is uniform, later centrifugal operation is convenient, and appearance of the final product is more uniform.

The invention shows a method for patch-clamp experiments on cells or similar structures, where at least one cell is inserted into the lumen of a capillary and is positioned inside the capillary so, that a sufficiently tight seal with a resistance exceeding 1, preferably 10 GigaOhm develops between cell membrane and inner surface of the capillary. Along its length said capillary has at least at one position a smaller inner diameter than the outer diameter of said cell. Preferably the cell is inserted and positioned in the capillary by pressure, suction, sedimentation or centrifugation of a suspension or solution containing said cell. An apparatus for performing experiments using this method is described.

The invention discloses a method for preparing a magnetic hybridized microsphere adsorption material for sewage treatment. The method comprises the steps: dissolving ferric nitrate and cobalt nitratein deionized water, adding modified attapulgite hybridized microspheres, carrying out uniform stirring and mixing, carrying out a heated refluxing reaction, and carrying out centrifugation, washing and baking, so as to obtain modified attapulgite-cobalt iron oxide magnetic hybridized microspheres; adding 2-methylimidazole and hollow zinc oxide microspheres into methanol, carrying out a stirred-mixed dissolution reaction, carrying out centrifugal separation and washing, and carrying out drying, so as to obtain zinc oxide composite microspheres; and adding cetyl trimethyl ammonium bromide, ultrapure water, anhydrous ethanol and ammonia water into nano ferroferric oxide, carrying out ultrasonic dispersion, then, dropwise adding tetrabutyl titanate, carrying out ultrasonic treatment, adding sunflower powder, carrying out magnetic stirring, carrying out magnetic field separation, then, carrying out washing, carrying out dispersing in acetone, carrying out heated refluxing stirring, carryingout magnetic field separation, carrying out alcohol washing, then, carrying out drying, carrying out heating-up treatment in a tubular furnace, carrying out cooling, and then, carrying out mixed ball-milling with the modified attapulgite-cobalt iron oxide magnetic hybridized microspheres and the zinc oxide composite microspheres, thereby obtaining the magnetic hybridized microsphere adsorption material.

The invention relates to a separation method for high-quality cashew proteins. The separation method comprises the following steps: (1) crushing and sieving cashew kernels, performing microwave treatment, and separating cashew oil by using a hydraulic cold-pressing method; (2) adding cashew meal in pure water, performing refining through a a colloid mill, and performing homogenizing treatment; (3) performing alkaline conditioning and dynamic ultra-high pressure treatment, performing centrifugation, extracting supernatant, regulating the pH to an isoelectric point, performing centrifugation, and washing precipitates with warm water; (4) performing homogenizing and spray-drying on the precipitates to obtain the high-quality cashew proteins. According to the separation method, process links are continuous and compact, the industrial production is easy, and products are high in yield and quality.

The invention discloses a preparation method of a silver-coated gold nanorod. The method comprises the following steps that a NaBH4 solution is prepared and is inserted into ice for at least 10 min; a cetyltrimethylammonium bromide (CTAB) solution is added into a container, the container is placed into a water bath at 30 DEG C, and stirring is performed at the speed not higher than 600 rpm; a HAuCl4 solution is added into the CTAB solution, then the NaBH4 solution is added under violent stirring at the speed not lower than 1000 rpm, and a brown seed solution is obtained; after 5 min, the seed solution stands in a water bath for 1 h; an AgNO3 aqueous solution is added into the CTAB solution under the condition of the stirring speed not higher than 600 rpm and the temperature being 30 DEG C, and then the HAuCl4 solution, the ascorbic acid solution and the seed solution are added to obtain a colorless mixed solution; the colorless mixed solution is stirred for 1 min and then stands in the dark for 1 h; the mixed solution obtained after standing is transferred into an Eppendorf tube, centrifugation is conducted to remove supernate, and solution sediment is obtained; the solution precipitate is resuspended in phosphate buffer saline water, and the gold nanorod is obtained; and a silver coating is formed on the surface of the gold nanorod.

A method for protein refolding using a like-charged ion exchange resins as an additive to facilitate protein refolding. The ion exchange resins mentioned herein includes charged group with the same sign as the target protein to be refolded and the method for refolding an unfolded protein. The method has some advantages described as below: firstly, no new impurity is introduced into the final product of protein due to ion exchange resins used in the present invention is insoluble and easy to remove by settlement or centrifugation, secondly; the key material used in the present invention, ion exchange resins, is easy to recycle after regeneration; thirdly, compared with the refolding method by chromatographic techniques, the current method requires no expensive chromatograph facilities and is simple to perform at lower cost, which is a desirable character for preparative refolding of therapeutic proteins expressed in bacteria as inclusion bodies.

The invention belongs to the technical field of material centrifugation, and discloses a horizontal spiral discharging centrifuge. The horizontal spiral discharging centrifuge is provided with a fixedbase, fixed shafts are symmetrically and fixedly installed at the upper end of the fixed base, a rotating device is installed on the two fixed shafts, the outer part of the rotating device is provided with spiral fan blades, the outer parts of the spiral fan blades are sleeved with a cylindrical outer shell, a solid collecting plate which is matched with the spiral fan blades is arranged on the inner wall of the cylindrical outer shell; a feeding device is fixedly installed at the front end of the cylindrical outer shell through a pipeline, a material leaking hole is formed in the rear end ofthe cylindrical outer shell, a liquid collecting device is installed at the discharging end of the material leaking hole; and the rotating device is in tooth connection with a speed regulating modulethrough a coupler. According to the horizontal spiral discharging centrifuge, rotary centrifugal separation is carried out in the horizontal spiral discharging centrifuge through the centrifugal principle, so that solids can leak out by being tightly attached to the cylindrical outer shell, liquid flows out and is collected through the liquid collecting device, a large number of solid-liquid mixed materials can be continuously separated, and the working efficiency is greatly improved.