37 results about "DNA" patented technology

A method for detecting human papillomaviruses (HPV) in biological material is described, in which method nucleic acids are extracted/isolated from biological material and HPV-specific DNA is detected in the isolated nucleic acids. This method uses at least one of the nucleotide sequences SEQ ID No. 1 or SEQ ID No. 2 from the enclosed sequence listing or sequences which bind to sequences to which one of the sequences SEQ ID No. 1 or SEQ ID No. 2 binds or sequences which are complementary to the abovementioned sequences.

The invention is directed to transcription activator-like effector nuclease (TALEN)-mediated DNA editing of disease-causing mutations in the context of the human genome and human cells to treat patients with compromised genetic disorders.

The invention discloses primers and a method for purity identification of hybrid seeds of Chinese pumpkin 'Guangmi NO.1'. The primers are VRISSRPMK8-F:5'-TAATTAAGGCGTGAAATTGG-3' and VRISSRPMK8-R:5'-GAAAAGAAACATAAACAGGGC-3'. The identification method is as follows: 1) extracting a genome DNA (deoxyribonucleic acid) of a pumpkin seedling; 2) carrying out PCR (polymerase chain reaction) amplification by taking the genome DNA of the pumpkin as a template and using the SSR primers VRISSRPMK8; 3) carrying out gel electrophoresis on the amplified product; and 4) analyzing the electrophoresis result, wherein only the single plant simultaneously having parent specific bands is a real hybrid, and the plant lacking of any one band is marked as false hybrid. The primers and the method can be used for effectively distinguishing the hybrid seed of the 'Guangmi NO.1' from the female parent and the male parent, and can identify the purity of the hybrid seeds rapidly and accurately. The method has the advantages of being fast and accurate, low in cost and simple in operation, and the like.

The present invention relates to a safe non-virulent Yersinia enterocolitica mutant strain for delivering heterologous proteins in target cells carrying mutations in at least one of the effector genes yopH, yopO, yopP, yopE, yopM, yopT genes and at least one additional mutation in the invasin genes chosen from yadA and/or inv. The present invention also relates to a safe non-virulent Yersinia enterocolitica mutant strain for delivering heterologous proteins in target cells according to claim 1 carrying mutations in all effector genes yopH, yopO, yopP, yopE, yopM, yopT genes and at least one additional mutation in the invasin genes chosen from yadA and/or inv. The present invention also relates to an expression vector for delivering a heterologous protein into a target cell using a Yersinia enterocolitica mutant strain according to any of the claims 1 to 4, which comprises in the 5' to 3' direction :(a) a promoter of a Yersinia virulon gene, (b) a first DNA sequence encoding a delivery signal from a Yersinia effector protein, operably linked to said promoter; and, (c) a second DNA sequence coding for said heterologous protein, fused in frame to the 3' end of said first DNA sequence. The present invention further relates to methods and compositions comprising (the use of) the afore-mentioned mutant strains and expression vectors.

Methods, reagents, compositions, and kits for in situ interaction determination (ISID) via interaction-dependent polymerase chain reaction (ID-PCR) are provided herein. ISID technology is useful for rapidly evaluating potential small molecule-target interactions from mixtures in a single solution. ISID is compatible with unpurified targets in biological samples and can be used to evaluate ligand-binding in DNA-encoded chemical libraries in cell lysates. ISID is also useful to screen ligand interactions of proteins or other molecules in their native state, including their native post-translational modifications and any interactions with accessory proteins and metabolites, in ways that better reflect their relevant biological environment. Because ISID is compatible with crude cell lysates, difficult-to-purify, poorly soluble, intrinsically unstable, and aggregation-prone targets may also be compatible with this method, without requiring truncation or other strategies used to promote heterologous expression.

The invention provides for the use of sulfur nucleophiles in analyzing methylated DNA and novel sulfur nucleophiles suitable for such us.

The invention discloses valid combinations of 8 novel SSR molecular markers and 2 known SSR molecular markers. The valid combinations are applied to strain identification. DNAs of 22 specific strains are taken as templates, 10 pairs of SSR primers are respectively utilized for carrying out PCR amplification, each pair of SSR primers is subjected to acrylamide gel electrophoresis after the PCR amplification, banding pattern statistic analysis is carried out according to all electrophoretic bands amplified from 22 samples, and therefore, a standard banding pattern of each pair of primers is determined; then the standard banding patterns of 10 pairs of primers of each sample are combined, and molecular identification cards of the 22 different strains of agaricus bisporus are constructed. According to an agaricus bisporus SSR molecular marker specific primer system and an application thereof, the strain identification effect of agaricus bisporus is improved; the agaricus bisporus SSR molecular marker specific primer system and the application are significant for the resource identification, the protection, the development and the utilization of agaricus bisporus.

Disclosed are methods for determining the immunological status of the adaptive immune system of a subject by identifying and quantifying rearranged DNA (and/or subsequently transcribed RNA) sequences encoding T cell receptor (TCR) and/or immunoglobulin (IG) polypeptides, in a lymphoid DNA-containing sample from the subject. TCR and/or IG sequence diversity and sequence distribution permit immunocompetence and immune repertoire assessment and reflect the degree of T cell or B cell clonality and clonal expansion in the sample. Methods for stratifying patient populations on the basis of immunocompetence including likelihood of responding to immunotherapy are also described.

A novel protein having inhibitory activity on the protease activity of hepatocyte growth factor (HGF) activator was purified and isolated, and its molecular weight (ca. 40,000 daltons) and its N-terminal and partial amino acid sequences were determined. A gene coding for the protein was cloned, and the gene DNA was incorporated into a vector, for transforming host cells. Cultivation of the transformant gave the desired protein. The protein can be used as an in vivo or in vitro control factor for HGF or HGF activator. It is also useful as an antigen to be used in producing an antibody to be used as means for kinetic studies of the protein, or as a standard in assay systems therefor.

The invention provides a detection method for genotyping of human platelet alloantigen (HPA). The detection method is characterized in that a target fragment is obtained by designing a specific primerand then using a single-tube multiplex PCR amplification reaction; a single base extension primer is designed and then single base extension is performed; then a target DNA sequence is accurately measured by mass spectrometry. The platelet alloantigen HPA-1 to 21 bw allele can be detected at one time. Then a mass spectrum is typed directly, and data are directly output. A whole process is closed,the time is short, and the cost is low. Through detecting the genotyping of the platelet alloantigen of a patient and a blood donor, platelets matching successfully can be found, and the problem of ineffective platelet infusion caused by immune factors is effectively avoided. A detection kit product is low in cost and more accurate, and the detection method is expected to be a routine clinical detection method to replace a serological detection method.

The invention provides an investigation method capable of realizing fast and precise investigation of space-time distribution and dynamic population change of released Chinese prawns. A detection method for released individuals is completed by specifically amplifying DNAs released by the Chinese prawns in a water environment, wherein the DNAs are mainly derived from the excrements and casts of theChinese prawns, an effective detection effect can be achieved on the Chinese prawns in any growth period, and there is no need to collect any Chinese prawn sample. Through real-time fluorescent quantitative PCR, the existence of the Chinese prawns can be judged, and the biomass of the Chinese prawns can be estimated according to the copy number of the DNAs released by the Chinese prawns. The space-time distribution and dynamic population change of the Chinese prawns can be rapidly and accurately detected in order to evaluate the effect of proliferation release.

The invention discloses a drought-resistant related SSR sequence derived from abnormal cotton and application thereof. The invention provides application of a specific DNA fragment or related biomaterials thereof in cultivation of drought-resistant cotton varieties or strains or improvement of the drought resistance of cotton. The specific DNA fragment is a fragment located on chromosome 5 of abnormal cotton and at least containing a sequence from an SSR molecular marker JAAS6365 (SEQ ID No. 1) to an SSR molecular marker JAAS5604 (SEQ ID No. 2). The related biomaterials are a vector, an expression cassette, recombinant bacteria or transgenic cell line containing the specific DNA fragment. The chromosome fragment provided by the invention has important application value in drought-resistantcotton breeding, and at the same time the SSR molecular marker developed by the invention provides a molecular basis for cultivation of drought-resistant cotton varieties that can be applied to production.

The invention discloses a rice secretary type thioredoxin gene, coded proteins thereof and application thereof and aims to provide the rice secretary type thioredoxin gene, the coded proteins thereof and a method for improving antioxidant activities of plants and delaying plant senescence by using the gene. The nucleotide sequence of the rice secretary type thioredoxin gene provided by the invention is a No.1 DNA sequence in a sequence table; and the amino acid sequence coded by the secretary type thioredoxin gene is a No.2 amino acid residue sequence in the sequence table. Experiments show that when the gene is excessively expressed in the rice, hydrogen peroxide content of a plant can be reduced and chlorophyll content can be improved in a normal state or in the presence of salts, so a rice senescence process is delayed. The rice secretary type thioredoxin gene, the coded proteins thereof and the application thereof have important application in aspects of improving the anti-oxidation and anti-aging performance of rice by using molecular breeding.

The invention provides an animal-derived ingredient detection method and application thereof. The method comprises the following steps: (1) constructing a database, (2) extracting DNA (deoxyribonucleric acid) from samples, (3) amplifying a Cytb gene sequence, and (4) carrying out MiSeq sequencing and sequencing analysis. According to the animal-derived ingredient detection method and the application thereof, provided by the invention, by the detection method, DNA of extracted samples is directly subjected to high-flux sequencing analysis, and thus, a sufficient data volume is guaranteed; due to designed universal primers, meat products mixed with several different varieties can be simultaneously determined, and the relative content of each variety in the meat products can be determined, sothat the rapid identification and the accurate determination of adulteration and doping of unknown animal-derived ingredients are achieved; and the method can be used for simultaneously carrying outanalysis on ingredients and components of 96 mixed samples, is high in sensitivity, simple in operation and short in detection time and has the detection limit of 0.01% and 0.1microgram.

Formation of nitrogen fixing root nodules in legumes is induced by perception of lipochitin-oligosaccharide signal molecules secreted by compatible Rhizobium bacteria, which triggers a common symbiotic pathway. The present invention provides a spontaneous nodule formation (snf1-5g) mutant, in which the formation of symbiotic nodules is deregulated, leading to nodule development in the absence as well as in the presence of Rhizobium bacteria and/or exogenous rhizobial signals. The invention further provides an isolated DNA sequence encoding a mutant chimeric Ca²⁺/calmodulin dependent protein kinase whose activity results in this `gain of function′ phenotype of spontaneous nodule formation. Furthermore the snf1-5g gene is shown to confer a spontaneous nodule formation phenotype to plants having a nodulation deficient genetic background. A gene of the invention, that confers a spontaneous nodulation phenotype, has utility for the transfer and establishment of nitrogen fixing capability in non-nodulating plants, and thereby reducing the nitrogen fertiliser dependence of non-nodulating crop plants.

The invention discloses a molecular marker of maize transcription factor ZmPIF3 transgenic rice and application of the molecular marker and belongs to the field of crop genetic breeding. A maize transcription factor ZmPIF3 transgenic rice material which is obtained already has obvious water-saving drought-enduring phenotype. According to a nucleotide sequence of maize transcription factor ZmPIF3 gene, a primer of the molecular marker is designed in CDS, PCR technology is applied to acquire DNA segments of ZmPIF3 and sequence the DNA segments, and the primer of the molecular marker is obtained according to specific segment screening of ZmPIF3. The molecular marker is high in repeatability, stable in amplification, simple and convenient to operate and low in cost and can be used for detecting maize ZmPIF3 genes in rice background, and a solid foundation is laid for molecular marker assisted selection in the future by utilizing ZmPIF3 gene transgenic rice.

The invention discloses an anti-counterfeiting code comprising two sets of final sequence information generated by subjecting identical sequence information to algorithm computing. The invention further discloses an anti-counterfeiting code generating system and an anti-counterfeiting code application system. Elements in the two sets of identical sequence information (similar to the dual-helix structure of DNA (deoxyribonucleic acid) are subjected to algorithm computing according to a set program, the final sequence information of uniqueness is generated, and the anti-counterfeiting code is generated according to the final sequence information; thus, the problem that anti-counterfeiting labels on products of manufacturers are easily faked is solved.

The invention provides a preparation process of a rabies vaccine without antibiotic addition in the whole process from a cytotoxic strain source. A Vero cell pre-master cell bank, a master cell bank,a working cell bank, a CTN-1V strain pre-master seed batch, a master seed batch and a working seed batch are prepared by using a culture medium without antibiotic addition, a bioreactor and a flaky carrier are applied, and through performing pre-culture and perfusion culture on Vero cells, inoculating a working seed batch of rabies viruses, performing washing and exchanging, performing perfusion culture to obtain a virus solution, performing ultrafiltration concentration, performing inactivating, performing purifying, preparing a semi-finished product, performing subpackaging and performing freeze-drying, the rabies virus vaccine is obtained. By optimizing the process of the virus adding course of bioreactor culture, impurity residues in the rabies vaccine are effectively reduced, the residual quantity of bovine serum components, Vero cell DNA and Vero cell host proteins is reduced, compared with other vaccine products on the market, the prepared vaccine product has superiority in clinical trial safety, and the safety of biological products is ensured to the greatest extent.

The invention discloses a molecular identification method for eleutheronema rhadinum and eleutheronema tetradactylum based on a COI gene sequence. The molecular identification method comprises the following steps of 1) extracting genome DNA of eleutheronema rhadinum and eleutheronema tetradactylum; 2) performing PCR amplification on cytochrome oxidase subunit I genes of eleutheronema rhadinum andeleutheronema tetradactylum by using an upstream primer and a downstream primer to obtain a PCR product of a target fragment; and 3) purifying the amplification products, directly sequencing, comparing the sequences of the amplification products of eleutheronema rhadinum and eleutheronema tetradactylum, determining that product is the eleutheronema tetradactylum if the 356-357th site base of the sequence is AA, and determining that product is the eleutheronema tetradactylum if the 356-357th site base is GG. The method can be used for rapidly, simply, accurately and effectively identifying eleutheronema rhadinum and eleutheronema tetradactylum, is stable in result and high in repetition rate, and plays an important role in aquaculture and fishery resource investigation.

The invention belongs to the field of identification of varieties and strains and particularly relates to DNA bar code primer composition for rapidly identifying a blastobotrys adeninivorans strain, DNA bar code composition, a kit, a method and an application. The DNA bar code composition comprises a first DNA bar code shown in SEQ ID No.1, a second DNA bar code shown in SEQ ID No.2, a third DNA bar code shown in SEQ ID No.3 and a fourth DNA bar code shown in SEQ ID No.4, wherein the first, second, third and fourth DNA bar codes are derived from a genome of the blastobotrys adeninivorans TMCC70007 strain. The DNA bar codes can rapidly identify the blastobotrys adeninivorans TMCC 70007 strain for fermentation of pu'er tea, and the strain can be rapidly and accurately identified from easily-confused strains or other strains of the same variety.

The invention discloses a molecular label suitable for detecting low-frequency variation of DNA by a next generation sequencing technology and application of the label. The following steps are involved. The molecular label comprises two Y-type DNA molecules, and each Y-type DNA molecule comprises a variable region, a first reverse complementation region, a random base region and a second reverse complementation region which are sequentially connected through phosphodiester bonds.

The invention provides a high-throughput detection method of an R ring. The high-throughput detection method comprises the following steps: firstly, closing free 3'-OH of DNA in a sample by using ddNTP; then, converting RNA in the sample into the DNA with biotin modification, and then performing DNA fragmentation and terminal repair; connecting joints; enriching the DNA with biotin modification by streptavidin magnetic beads; and performing chain-specific library amplification and sequencing. The invention provides a simple, convenient and rapid technology for detecting the R ring in the genome range, the R ring can be efficiently and accurately identified, and the whole process is simple to operate, short in time consumption (not more than 4 hours), wide in applicability, high in sensitivity and accuracy and suitable for development and application of R ring detection commodity kits.

A method for developing and extracting biological trace evidence comprises the following steps: (1) using a biological fluorescent development reagent to process a porous carrier so as to develop biological trace evidence on the porous carrier, wherein a raw material formulation of the biological fluorescent development reagent is, in percent by weight: 0.01%-0.5% of indanedione, 4%-10% of ethyl acetate, 0.5%-1.5% of glycerol, 5%-15.5% of pure alcohol, and 73.5%-90% of petroleum ether; and (2) extracting the biological trace evidence to obtain DNA information of the biological trace evidence. For crime investigators who need to extract DNA evidence, the method enables targeted extraction of physical evidence, thereby greatly reducing workload, and furthermore, the method can also be used to develop and extract obscure or trace evidence, such as a fingerprint on a garment, thereby greatly improving investigation efficiency.

Disclosed is a method for providing information used for comparing restoration rates of damaged DNA, wherein information about a time period when Ataxia telangiectasia and Rad3 related (‘ATR’) activation is accelerated, on the basis of alternative information about an expression level of cryptochrome may be acquired, therefore, it can be determined that a restoration rate of damaged DNA is high at a time period when the expression level of cryptochrome is high. Accordingly, a time period when a restoration rate of DNA damaged by different causes is high, can be determined. Further, it is possible to estimate a time period when side effects occurring due to using an anticancer drug are minimized, and then, utilize the estimated result in determining the timing of administration of an anticancer drug.