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7 results about "Negative charge" patented technology

Compound biochip based on photon crystal

InactiveCN101358242AImprove performanceEnhanced signal selectivityMicrobiological testing/measurementMicrosphereComposite substrate
A making method of a composite type biological chip based on a photonic crystal comprises four steps which are preparation of hard substrates, preparation of photonic crystal thin membranes, connection of membranous substrates and surface treatment of the substrates. (1) the preparation of hard substrates: common hard substrates are cleaned by soapy water, washed by de-ionized water and dried, and amino-group or hydroxyl is decorated on the surfaces of the substrates; (2) the preparation of photonic crystal: the nano-microspheres such as specific monodisperse silicon dioxide or polystyrene and the like, which are different in size according to the positions of testing signals, are assembled on the surfaces of the treated substrates to form the photonic crystal thin membrane with the thickness of 20 to 30 microns (3) the connection of membranous substrates: the surface of the photonic crystal thin membrane is laid with a layer of the membranous substrate and the connection is fixed by utilizing the chemisorption or the mutual function between positive and negative charges; (4) the surface treatment of the substrates: by using the methods such chemisorption and the like, chemical groups which can be fixedly connected with biomolecules to be tested are decorated on the membranous layer parts of the surfaces of the composite substrates which are formed in the step (3).
Owner:SOUTHEAST UNIV

pH-sensitive modified chitosan transgene vector, preparation method and applications thereof

InactiveCN106279464AEasy to compressEfficient compressionOther foreign material introduction processesBackbone chainGene vector
The invention discloses a pH-sensitive modified chitosan transgene vector, a preparation method and applications thereof, wherein agmatine and dimethyl maleic anhydride are respectively grafted on the main chain of a chitosan macromolecule to form a biological macromolecule simultaneously having positive charge and negative charge, and the prepared material is used for gene transfection. According to the present invention, the preparation method is simple, the reaction condition is mild, the obtained vector can effectively compress DNA under the neutral condition to form the surface-charged nanometer complex so as to easily perform the adsorption of the complex antiserum protein, and under the acidic pH condition, the charge reversal occurs, and the surface charge of the nanometer complex is changed to the positive from the negative so as to easily achieve the cell endocytosis of the complex, such that the transfection efficiency is improved.
Owner:TIANJIN UNIV
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