32 results about "Protein" patented technology

Methods and devices for microfluidic detection of a biological maker in a biospecimen collected from a subject are disclosed. The microfluidic devices include nanoparticle-based nanosensors comprising supramolecular recognition sequences, protease consensus sequences, post-translationally modifiable sequences, or sterically hindered benzylether bonds for specific interaction with a biological marker. Also disclosed are particular nanosensors for detecting cytokines, and other proteins based upon supramolecular recognition without chemical modification or enzymatic cleavage.

The invention belongs to the field of protein analysis, and relates to a new method for enrichment of a glycosylated peptide through a magnetic nanometer material modified by aminoxy, in particular to a method for solid-phase enrichment and mass spectrographic analysis of a glycosylated peptide fragment. The method comprises the following steps: firstly, using sodium periodate NaIO4 to oxidize a hydroxyl group on a glycopeptide to change the hydroxyl group to an aldehyde group; secondly, placing the magnetic nanometer material Fe3O4@ONH2 modified by aminoxy to an oxidized glycopeptide solution, and fixing the glycopeptide to the magnetic nanometer material through reaction of an aminoxy group and the aldehyde group obtained after a sugar chain in the glycopeptides is oxidized; thirdly, cleaning to remove non-glycopeptide which does not react with the nanometer material; finally, dissociating the captured glycopeptide from the material by use of sugar chain enzyme, and realizing mass spectrographic analysis of the glycosylated peptide. The method is simple in step, convenient to operate, high in speed and efficient, and can realize highly-sensitive and highly-selective mass spectrometric analysis of the glycosylated peptide.

The invention relates to a pulmonary tuberculosis variation activity marker, a kit, a method and a model construction method, and the pulmonary tuberculosis variation activity marker is characterized in that the pulmonary tuberculosis variation activity marker is a plasma protein biomarker in a blood sample, and the plasma protein biomarker is a combined marker comprising one or more of C1QB protein and CCL19 (MIP3B) protein; a combined marker of C1QB and CCL19 (MIP3B) is used as a biomarker for detecting the activity of pulmonary tuberculosis for the first time, a rapid diagnosis model for detecting the activity of pulmonary tuberculosis is constructed, a new direction is provided for clinical diagnosis of tuberculosis, and as a technical conception for diagnosing the activity of pulmonary tuberculosis, the biomarker provides a new target spot for the diagnosis of the active tuberculosis; therefore, the invention overcomes the defects of low detection rate, long time consumption and the like of the existing active tuberculosis diagnosis, has the characteristics of good specificity and high sensitivity, and has good clinical application value for auxiliary diagnosis of tuberculosis activity.

The invention relates to a sheep placenta peptide extraction method, which comprises steps of cleaning, digesting, discharging soup, hydrolyzing, filtering, concentrating and spray-drying. With the application of the extraction method disclosed by the invention, problems on the effective absorption and utilization of macromolecular protein in human body are effectively solved, and micromolecular transformation and extraction of the protein are achieved. Rich tissue protein in sheep placenta is transformed into micromolecular peptide through directional biological enzymolysis; the protein, which is derived from an animal body, is quite close to human body in structure and function, and the protein, which has a micromolecular structure, can be directly absorbed by intestines without digestion and can rapidly participate in normal metabolism and regulation of the human body; therefore, the protein, in pharmacology, is higher in biochemical mechanism reasonability and specific treatment effectiveness. The sheep placenta peptide has the characteristics of being strongly targeted, free from toxic and side effects, significant in curative effect, high in nutritive value, easy to to be absorbed by human body and the like.

A production method of burnt alcohol comprises the following steps: 1) pretreating malt: sequentially carrying out malt soaking, draining, protein resting, saccharifying and operating; (2) stir-fryingburnt malt: stir-frying the malt treated in step 1) in three steps of saccharifying, heating for baking burning and stir-frying burning, wherein the stir-frying burning is carried out at 270 DEG C orabove for 5 min or less; and 3) extracting burnt alcohol for 8-10 h by adopting ethanol with the alcohol concentration of 30-32%, at a soaking temperature 40 +/-2 DEG C and a solid-to-liquid ratio of50 g malt/100-200 ml ethanol. The alcohol with low concentration (30-32%) and medium-low temperature (40 +/-2 DEG C) is adopted to soak the stir-fried burnt malt in order to effectively extract pyrazines, furans, furanones, pyrone compounds and other burnt aroma components in the burnt malt, so that the burnt alcohol prepared by the production method of the burnt alcohol is rich in aroma components with various burnt fragrances, rich in burnt flavor, small in dosage and proper in flavor modulation.

A label that permits rebridging of disulfide linkages in antibodies or proteins. The label has a general formula given by

wherein R₁ is methyl, ethyl or propyl and R₂ is a metal chelator, a fluorophore or a click-chemistry synthon.

The invention discloses a food containing rich asparagus protein and belongs to the field of food processing. A minced-fish product comprises the following components in parts by weight: 10 parts of fish meat, 6-10 parts of potato starch, 10-15 parts of asparagus protein, 3-6 parts of granulated sugar, 2-4 parts of salt, 0.2-0.4 part of transglutaminase, 0.5-1.0 part of carrageenan, 0.4-0.8 part of sodium bicarbonate, 0.4-0.8 part of composite phosphate, 5-10 parts of iced water and 0.7-1.0 part of seasoning liquid. The asparagus is crushed by using a masher, alkali extraction and acid precipitation are utilized for extracting the asparagus protein. The minced fish product containing rich asparagus protein is rich in nutrition, high in protein content, resistant in storage resistance, good in gelling property and good in mouthfeel, well maintains the freshness of raw fish, can provide stable-quality materials for the processing industry of the minced fish product and has wide market prospect.

The invention discloses application of bitter gourd exosomes in radiation heart injury protection, the bitter gourd exosomes and rat myocardial cells (H9C2) are co-cultured and subjected to radiation treatment, MTT experiments show that 5-25 [mu] g/ml of the bitter gourd exosomes can promote the cell viability of the radiated H9C2 myocardial cells, and 10 [mu] g/ml of the bitter gourd exosomes are selected as the use concentration of the bitter gourd exosomes. Under the condition that the bitter gourd exosome is 10 [mu] g/ml, Ki-67 fluorescent staining proves that the bitter gourd exosome can promote proliferation of radiated H9C2 myocardial cells. An Annexin V-PE/7-AAD flow apoptosis experiment and an apoptosis protein cleaved-caspase3 immunoblotting test prove that the bitter gourd exosome can be used for reducing the apoptosis of H9C2 myocardial cells after radiation. Immunofluorescence staining and protein immunoblotting of a DNA damage marker gamma-H2A. X show that the bitter gourd exosome can reduce DNA damage of H9C2 myocardial cells after radiation.

The invention provides a method of recovering sweet potato starch wastewater protein. The method includes the following steps that sweet potato starch wastewater is collected and filtered with a filter screen to remove large particle impurities and starch granules; pathogenic bacteria are removed, and then the pH value of the wastewater is adjusted to be 9.5-10.5; a chitosan acid solution is addedinto the treated sweet potato starch wastewater, and stirring and standing are carried out; a precipitate is subjected to centrifugal treatment, a supernatant is discarded, and then the precipitate is dried to make the moisture content of the precipitate lower than 20 wt%; the pathogenic bacteria are further removed by means of an ultraviolet disinfection approach, and a high-protein recovered substance is obtained. The method can solve the problem of pollution of sweet potato starch wastewater in China to a large extent; at the same time, the useful component protein in the wastewater is recycled to produce high-protein animal feed, and thus waste is turned into treasure.

Methods for the preparation of polymeric films which encase and preserve bioactive agents. In particular, the invention is directed to the preparation of oral thin films containing bioactive proteins or viruses. For example, methods and compositions are disclosed for preservation of rotavirus and antibodies in thin dry films.

The invention discloses a mutant for cellobiohydrolase. The mutant is obtained by mutating amino acid of at least one selected from the group consisting of 133rd, 165th and 183rd sites of the cellobiohydrolase with an amino acid sequence of SEQ ID NO.1 into alanine. According to the invention, a mutant protein is obtained by performing protein engineering transformation on the cellobiohydrolase. The mutant provided by the invention has higher enzyme activity under same conditions, can greatly improve the degradation efficiency of cellulose, and promotes application in the field of cellulose degradation.

The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands. Finally, the field of the invention also encompasses multi-omic MSI procedures, where MSI of photocleavable mass-tag probes is combined with other modes of MSI, such as direct label-free MSI of endogenous biomolecules from the biospecimen (e.g., tissue), whereby said biomolecules can be intact or digested (e.g., chemically digested or by enzyme).

A TPR1 gene related to a low-temperature tolerance of Pomacea, coding protein and application of the gene is disclosed. The disclosure belongs to the field of biotechnology. The present disclosure is rapid, effective and reproducible, and is an important complement to the TPR1 gene family. Pomacea is an important invasive organism, the disclosure is helpful for developing novel biological pesticide, and the low-temperature tolerance of the organism is reduced by blocking the expression of TPR1 gene in Pomacea, so as to control the further northward invasion of Pomacea. Meanwhile, by interfering with the expression of TPR1 gene, the hatching rate of Pomacea eggs can be significantly reduced, and the hatching period of eggs can be prolonged, thereby reducing the harm of Pomacea invasion.

The invention belongs to the technical field of oligoasthenozoospermia and asthenozoospermia diagnosis, and particularly relates to application of MYH9 in preparation of a diagnostic reagent for severe oligoasthenozoospermia and asthenozoospermia. On the basis of a proteomics method, protein data in normal and severe oligoasthenozoospermia and asthenozoospermia samples are analyzed, and the protein abundance in the samples is detected by taking BSA protein signal intensity as a standard to obtain differential protein in normal and diseased samples. The MYH9 is detected and confirmed to be significantly up-regulated in severe oligoasthenozoospermia and asthenozoospermia samples through the method, has good credibility, is expected to be used as a detection marker of severe oligoasthenozoospermia and asthenozoospermia, and is applied to development of related detection reagents and kits.

The present invention belongs to the field of biotechnology. More specifically, the present invention provides a protease, a non-naturally occurring fusion protein comprising a corresponding protease recognition site, expression vectors encoding same, host cells comprising said expression vectors, kit of parts as well as methods applying the protease, fusion protein, and uses thereof, as defined in the claims. The presently disclosed protease/protease recognition site is particularly useful in methods requiring an orthogonal set of proteases, and is suitable for use in both prokaryotic and selected eukaryotic expression systems

The present invention provides compositions, such as varnishes, ink vehicles, and finished inks, having high bio-renewable carbon (BRC) content. The compositions comprise protein and colophony.

The invention discloses a height increasing traditional Chinese medicine pellet, and belongs to the technical field of traditional Chinese medicine, bulk drugs comprise the following traditional Chinese medicine components: ginseng, American ginseng, dogwood, cornu cervi pantotrichum, schisandra chinensis, eucommia ulmoides, achyranthes bidentata, ophiopogon root, rhizoma drynariae, codonopsis pilosula, astragalus membranaceus, prepared rehmannia root and the like, and pharmaceutic adjuvants comprise starch, lactose and chitosan-benzoic acid-polyaspartic acid cross-linked polymer. According to the height-increasing traditional Chinese medicine pellet disclosed by the invention, active ingredients of the radix achyranthis bidentatae and the human placenta are extracted and then are directly mixed with other crushed active ingredients to prepare the pellet, so that the pellet is convenient to take, children do not generate rejection psychology when taking the pellet, and the problem that the existing traditional Chinese medicine decoction is poor in taste is solved; the height-increasing traditional Chinese medicine pellet adopts medicinal and edible traditional Chinese medicinal materials, contains various nutrient substances, such as protein, amino acid, vitamins and various trace elements, required by height increase of a human body, can provide nutrient substances for the body, promote metabolism of the body, regulate various physiological functions of a physiological system and enhance immunity, and can improve physique after being taken persistently. The body development and the skeleton growth are promoted.

This invention provides a method for detecting cognitive disfunction diseases including mild cognitive impairment and Alzheimer's disease using a protein and a peptide of the protein different in the presence level in subjects having a normal cognitive function and patients suffering from cognitive disfunction diseases and a biomarker for detecting cognitive disfunction diseases including mild cognitive impairment and Alzheimer's disease containing the protein and the peptide. This invention is a biomarker for diagnosing cognitive disfunction diseases containing a prothrombin precursor protein of SEQ ID NO: 1 or a peptide THRB containing the amino acid sequence represented by SEQ ID NO: 2 which is a peptide of the protein, a diagnosis method for cognitive disfunction diseases using the biomarker, an antigen peptide represented by SEQ ID NO: 3 for creating a THRB peptide specific antibody to be used in the diagnosis method, and a cognitive disfunction disease diagnosis kit containing the THRB peptide specific antibody.