37 results about "Biochemistry" patented technology

The invention relates to a supercritical extraction method of a conic gymnadenia tuber element, and in particular relates to a method for extracting a natural active ingredient with an anti-aging effect, i.e. the conic gymnadenia tuber element, from a Tibetan medicine conic gymnadenia tuber. According to the invention, through creating a series of new extraction process conditions of pressure, temperature, entrainer and the like and the method, the traditional extraction contradiction of active ingredient purity and extraction rate are solved. Anti-aging medicines, health care products and human hand and face skin care products, which are manufactured through taking the conic gymnadenia tuber element as a basic formula, have the characteristics of nature, safety, transdermal absorption, no side effect and the like.

The invention discloses an engineered ketoreductase polypeptide and a method for preparing a montelukast midbody by means of the same. Compared with the prior art, the engineered ketoreductase polypeptide has the advantages that enzyme consumption is low, reaction time is short, substrate concentration is high, and the engineered ketoreductase polypeptide is more suitable for industrial application when used for preparation of the montelukast midbody.

The invention discloses a kit for detecting valproic acid drug concentration in dry blood spots. The kit comprises a kit body, a first container, a calibrator card and a quality control card, whereinthe calibrator card is a blood spot collection card fixed with a group of valproic acid dry blood spot samples as dry blood spot calibration points, and the number of the dry blood spot calibration points is at least six, the quality control material card is a blood spot collection card fixed with at least one group of valproic acid dry blood spot samples as dry blood spot quality control points,the number of each group of dry blood spot quality control points is at least three, the first container is filled with an internal standard extracting solution, and an internal standard adopts isotopic substituted valproic acid. The invention further discloses a detection method of the kit. The kit provided by the invention has advantages of simple preparation of reagents, less required amount ofsamples, convenience in storage and transportation, rapid pretreatment, short analysis period, lower cost and the like, and is suitable for popularization in clinical conventional detection.

The invention relates to the field of pepper processing and specifically discloses a method for purifying natural capsaicin. The method comprises the following steps: decomposing the pepper into three parts, including embryo tissues, peels and pepper seeds; respectively smashing the three parts; adding a corresponding amount of cellulase into the smashed tissues; stirring and mixing at different rotating speeds and for different stirring durations; accelerating the damage to cells; accelerating the separation of capsaicin; extracting the capsaicin from the pepper tissues; dissolving the capsaicin in a solution; and then separating capsaicin crystals from the solution, thereby completing the whole capsaicin extracting and purifying processes. According to the scheme, the pepper is divided into three parts and the three parts in different capsaicin contents are respectively processed, so that the separation of the capsaicin as much as possible can be guaranteed.

The invention relates to an ANGPTL4 polypeptide for use in the preservation of vascular endothelial cell barrier integrity and reduction in no-reflow phenomenon with myocardial infarction.

The invention relates to the technical field of bronchoalveolar lavage, and aims to provide in-vitro lung bronchoalveolar lavage fluid and an acquisition method thereof. The bronchoalveolar lavage fluid obtained for the in-vitro lung contains rich biological information, and the obtaining method is harmless to a patient and free of danger and meets ethical requirements.

The invention relates to the technical field of biology, in particular to a polypeptide, a preparation method of the polypeptide, a bispecific antibody and application of the bispecific antibody. The polypeptide is an Fc binding polypeptide, and can be universally, simply and conveniently connected with a monoclonal antibody to prepare a bispecific / multispecific antibody. The polyvalent Fc binding polypeptide PGLU-Fc-III-4C or PASP-Fc-III-4C is prepared by bonding side carboxyl groups of polyglutamic acid (PGLU) or polyaspartic acid (PASP) with Fc-III-4C polypeptide which has general high affinity to an immune globulin (antibody) constant region (Fc), and the polyvalent Fc binding polypeptide can react with various monoclonal antibodies in different proportions in an aqueous solution to obtain a bispecific / multispecific antibody. The preparation method of the bispecific / multispecific antibody has the following advantages: the bispecific / multispecific antibody is prepared by direct incubation in a water environment, the reaction condition is mild, and the activity of the monoclonal antibody can be retained to the greatest extent; and the method is simple, efficient, controllable in monoclonal antibody proportion and high in universality, and has an industrialization prospect.

The present invention relates to a method for determining an analyte in a sample, comprising a) contacting said sample with at least a first and a second detector compound; b) determining the amount of complexes comprising at least one detector compound; and, c) determining said analyte in a sample based on the result of step b), wherein said first detector compound comprises a first binding moiety and a first label, and said second detector compound comprises a second binding moiety and a second label, and wherein the first label and the second label are non-identical. The present invention further relates to a kit for detecting an analyte in a sample, comprising at least a first and a second detector compound for said analyte, wherein said first detector compound comprises a first binding moiety and a first label, and said second detector compound comprises a second binding moiety and a second label, and wherein the first label and the second label are non-identical; and to a device for determining an analyte in a sample, comprising at least a first and a second detector compound for said analyte, wherein said first detector compound comprises a first binding moiety and a first label, and said second detector compound comprises a second binding moiety and a second label, and wherein the first label and the second label are non-identical; and means for determining at least one signal obtained from said first label and said second label; and to the use of a composition comprising at least a first and a second detector compound for detecting an analyte, wherein said first detector compound comprises a first binding moiety and a first label, and said second detector compound comprises a second binding moiety and a second label, and wherein the first label and the second label are non-identical.