8 results about "Monoclonal antibody" patented technology

The invention discloses a human Lp-PLA2 biotin-streptavidin fluorescence immunochromatographic assay card and a preparation method thereof and aims at providing an assay card which is supportive of quick combining, free of outside interference during reaction and high in specificity and stability and a preparation method thereof. The technical scheme includes that assay test paper comprises a substrate, a sample pad, a mark pad, a coated film and an absorption pad are connected on the substrate. The assay card is characterized in that an anti-Lp-PLA2 monoclonal antibody 1 of fluorescent microspheres and a biotin anti-Lp-PLA2 monoclonal antibody 2 are coupled on the mark, a streptavidin assay line T and a goat-anti-mouse polyclonal antibody quality control line C are parallelly arranged on the coated film. The invention belongs to the field of fluorescence immunochromatographic assay.

The invention discloses a method for quickly detecting aflatoxin B1 by PbS quantum dot. The method is characterized by comprising the steps of: adding Pb(NO3)2 solution into mercaptoacetic acid and Na2S solution to prepare PbS quantum dot; preparing 10mug/mL QD-Ab solution by coupling of PbS quantum dot and aflatoxin B1 monoclonal antibody; adding aflatoxin B1-bovine serum albumin conjugate in an elisa plate to coat the elisa plate, and simultaneously adding AFB1 solution and QD-Ab solution, after incubation digestion, adding Hg2Cl solution, getting constant volume by adding acetic acid buffer solution, then preparing the to-be-detected solution; deoxidizing the to-be-detected solution, adding the deoxidized to-be-detected solution into a pretreated glassy carbon electrode for detection, and calculating the content of the aflatoxin B1 according to a standard curve relation between the dissolving peak size of Pb<2+> and the concentration of aflatoxin B1 solution. The method has the advantages of high sensitivity, strong selectivity, excellent stability, high accuracy and quick detection speed.

The invention provides a test kit and a preparing method thereof for rubella IgG antibody detecting by integrating chemiluminescence immunoassay with capture method. The test kit is composed of positive and negative reference substance, a micro-perforated plate which is coated by antihuman IgM u link, neutralizing antigen, sample diluent, condensed washing liquid, alkaline phosphatase labeled monoclonal antibody, and chemiluminescence zymolyte. The preparing method of the test kit includes: 1) the positive and negative reference substance is prepared; 2) the micro-perforated plate is coated by the antihuman IgM u link; 3) the neutralizing antigen is prepared; 4) the sample diluent is prepared; 5) the condensed washing liquid is prepared; 6) the rubella monoclonal antibody is labeled by the alkaline phosphatase; 7) the chemiluminescence zymolyte is prepared; 8) all components above can be filled and packed through separate packing and finished product can be made after assembly. The invention has the advantages that the sensitivity is high and the specificity is strong, thereby providing serology detection, epidemiology investigation and clinical diagnose with a reliable scientific diagnosing evidence which is helpful in eugenics.

The invention belongs to the technical field of immune assay medical detection and particularly provides a PIVKA-II (protein induced by vitamin K absence-II) magnetic particle chemiluminescence immuneassay determination kit and a preparation method thereof. The kit mainly comprises (1) a PIVKA-II standard product; (2) anti-FITC (fluorescein isothiocyanate) monoclonal antibody coated magnetic particles; (3) an alkaline phosphatase marked PIVKA-II sample and fluorescein isothiocyanate marked PIVKA-II; (4) an alkaline phosphatase marked chemiluminescent substrate; (5) subpackage of the standardproduct, magnetic particle solution, mixed solution, sample dilution liquid, washing liquid, the chemiluminescent substrate and reaction tubes. By combination of the enzyme marking technique, the magnetic particle separation technique and the chemiluminescence detection technique, an approximately homogenous reaction system is provided, and simplicity, sensitivity, high specificity, wide linear range, high repeatability and the like are realized.

The present invention is directed to KIAA1199, which is a novel factor involved in decomposition of hyaluronic acid, and to use thereof. More specifically, the invention is directed to a hyaluronic acid decomposition-promoting agent containing the KIAA1199 gene and a protein encoded by the gene; to a hyaluronic acid decomposition-inhibiting agent characterized by inhibiting the activity or expression thereof (including an siRNA or a monoclonal antibody); and to a method for screening a novel hyaluronic acid decomposition-controlling agent, in which the method contains employing the expression of KIAA1199 as an index.

The invention discloses a test strip for detecting an organophosphorus pesticide and application thereof. The test strip comprises a sample absorption pad (1), a conjugate release pad (2), a reactionfilm (3), a water absorbent pad (4) and a bottom plate (7), and the reaction film comprises a detection line (5) coated with an organophosphorus pesticide hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse antibody, the conjugate release pad (2) is sprayed with an organophosphorus pesticide monoclonal antibody-colloidal gold label. The invention also provides a method for detecting organophosphorus pesticide residues in crops such as vegetables and fruits by using the test strip for detecting the organophosphorus pesticide. The test stripn has the characteristics of simple operation, high sensitivity, fast detection speed and low cost, and is suitable for screening and on-site monitoring of a large number of samples.