40 results about "Repeatability" patented technology

The invention discloses a cleaning fluid applied for automatic biochemical analyzers, comprising: at least one anion surface active agent, at least one nonionic surface active agent, an alkali metal hydroxide, an alkali metal citrate and a buffer agent for stabilizing pH value above 13.0. The cleaning fluid has low protein residual rate, low fatty material residual rate, good in-batch repeatability, low cross pollution and low reactant deposition rate in clinical application after cleaning. And the cleaning fluid will not affect the test result of biochemical analyzers, and has not corrosion on the liquid circuit system and reaction cup of analyzers. All components of the cleaning fluid can be degraded biologically, to avoid potential environment pollution and meet environment protection requirement.

The invention provides a cross writing and drawing robot which comprises a left base and a right base, wherein a transverse polish rod is arranged between the left base and the right base, and is movably provided with a moving center; a longitudinal polish rod vertical to the transverse polish rod is arranged on the moving center; a penholder module and/or a laser module are/is arranged at the end part of the longitudinal polish rod; and a left motor and a right motor are respectively arranged on the left base and the right base, and can be used for driving the penholder module, the moving center and the longitudinal polish rod to make X-axial movement on the transverse polish rod, or driving the penholder module and the longitudinal polish rod to make Y-axial movement relative to the moving center. The cross writing and drawing robot can be used for automatically controlling the penholder module to make X-axial movement or Y-axial movement through a control module, as well as writing or drawing by virtue of a pen clamped on the penholder module instead of a person, has the advantages of compact structure, reliable performance, sensitive action, high movement displacement accuracy, long service life and the like, and is suitable for working situations with relatively high repeatability.

The invention discloses a rat acceleration and deceleration brain trauma striking system which comprises an experimental platform, a striking module, a striker module, a positioning module and an acceleration and deceleration trauma switching module. The positioning module is longitudinally and perpendicularly arranged on the experimental platform, the striking module is transversely and perpendicularly arranged on the positioning module, the striking module is placed on the experimental platform, the striker module is arranged at the position, arranged between the positioning module and the striking module, of the experimental platform, and the acceleration and deceleration trauma switching module is arranged on the side edge, longitudinally and perpendicularly corresponding to the striking module, of the experimental platform. Compared with the prior art, the system can precisely manufacture a rat brain trauma model, the repeatability is good, the operation is easy, a rat does not easily die, the acceleration brain trauma model and a deceleration brain trauma model can be manufactured, trauma grading can be carried out on the manufactured models, a matched group of a brain opening cavity and a brain non-opening cavity can be manufactured, and a large amount of support can be provided for research for the human brain trauma.

The invention discloses a rapid and high efficiency chlorogenic acid and galuteolin in honeysuckle flower extraction method and a HPLC quantitative analysis method, and which comprise the following steps: superhigh pressure extraction and quantitative analysis. Superhigh pressure extraction comprises the steps as follows: firstly crushing dried honeysuckle flower and filtering by a sieve with 60-80 meshes, adding water or ethanol, methanol and n butanol organic solvent into the honeysuckle flower powder according to 1:10-1:100 solid liquid ratio, mixing and sealing, immersing at normal temperature, placing the mixture into a high pressure container, leaching with pressure at 20-60 DEG C. for 1-5 min, relieving pressure, carrying out centrifugation of extract product and obtaining the honeysuckle flower extract. Quantitative analysis: after centrifugation, diluting 2ml of the obtained supernatant to 10 ml with distilled water in a volumetric flask, refrigerating for standby. The chlorogenic acid and galuteolin in honeysuckle flower are extracted by a superhigh pressure technology and are carried out by a content determination, and the invention has the characteristics of high recovery rate, short extraction time, small solvent amount, simple operation, accuracy, good repeatability, no pollution, safety, low energy consumption, etc.

The invention provides a monitoring method of the internal stress of a spacecraft composite material structure in an aging process. The method includes following steps: step1, placing carbon fiber prepreg, a special optical fiber, carbon fiber prepreg, demolding cloth, and adhesive-absorbing felt on a molding mold of the spacecraft composite material structure in sequence, and performing integral sealing by employing a vacuum bag; step 2, performing curing by employing an autoclave process, performing demolding after cooling, and obtaining the spacecraft composite material structure implanted with the special optical fiber at the inner part; and step 3, placing the spacecraft composite material structure in a clean factory building, and performing aging treatment on the spacecraft composite material structure by employing a natural aging method. According to the method, based on a back Rayleigh scattering principle, the special optical fiber is implanted in the spacecraft composite material structure, the monitoring of the internal stress in the aging process is realized by employing an optical frequency domain reflection technology, the spatial resolution is high, the measuring precision is high, the repeatability is good, and basis is laid for improvement of the aging method of the spacecraft composite material structure and the improvement of the dimension stability.

Belonging to the field of chemical pharmacy, the invention relates to metal complexes, in particular to a semi-sandwich structure iridium complex with anticancer activity, a preparation method and application thereof. The iridium complex has a molecular structural formula shown as the specification. According to the invention, P^O is selected as a bidentate chelate anionic ligand, and the synthesized iridium complex is a semi-sandwich structure, has high anticancer activity, little side effect, and high inhibition rate on cervix carcinoma cell (HeLa) growth. The preparation method provided bythe invention has the advantages of simple process, low cost, easily controllable chemical components, good repeatability, high yield and the like. The introduced [P, O] neutral ligand in the invention endows the complex with high anticancer activity, and the invention provides a new research idea for the synthesis research of follow-up anticancer drug ligands.

The invention discloses an oakleaf hydrangea cutting propagation method, and belongs to the technical field of plant propagation. The method comprises the following steps that 1, an appropriate propagation field is selected to build an oakleaf hydrangea female parent cutting orchard; 2, a greenhouse and an overhead seedbed are adopted for cutting; 3, the appropriate cutting time is the first or second ten-day period of a month, and cutting is carried out one time a year; 4, cutting branches are soaked in a root promoting agent; 5, about 75% of scion wood roots 60 days after cutting, and then seedling exercising is carried out. According to the oakleaf hydrangea cutting propagation method, operation is easy, the propagation speed is high, the yield is as high as 80%, and the method is reported for the first time in China, fills a gap in the relevant technical fields, can be used for large-scale production, is good in repeatability and has the quite good market prospect.

The invention provides a wheat scab resistance identification method. Wheat scab resistance is identified in a short time at high throughput in case of controllable environmental conditions by utilizing in-vitro ears of wheat. The method disclosed by the invention has the advantages that a novel scab resistance evaluation standard is discovered, and the wheat scab resistance is evaluated accordingto whether black dense necrotic spots are produced at inoculation positions. By utilizing the standard, limitations of external environmental conditions can be avoided, and the wheat scab resistancecan be simply and rapidly identified within 4-5 days. The method is intuitive and reliable in phenotype identification and excellent in repeatability, is capable of completing resistance identification of batches of materials in a short time, and has significances on genetics and breeding researches of wheat scab resistance all over the world.

The invention discloses a device and method for measuring the contents of oxygen, nitrogen and part of organic gas in mixed gas. The method comprises the following steps: a mixed gas is introduced into a sample through a six-way valve; organic gas is detained through a Q column, residual oxygen and nitrogen are detected through a thermal conductivity cell (TCD) detector, a spectrogram is obtained,then the organic gas detained through the Q column is detected through a hydrogen flame (FID) detector, the spectrogram is obtained, and finally the peak areas of all components in the two spectrograms are measured through gas chromatography, so that the content of all the gases is obtained. The method disclosed by the invention is high in precision and accuracy, good in repeatability and simple,convenient and rapid to operate.

The invention discloses a method for rapid determination of isothiazolinone compounds in a water-based adhesive for cigarettes. The method comprises the following steps: adding an extraction agent into the water-based adhesive for cigarettes, wherein a volume mass ratio of the extraction agent to a water-based adhesive sample is 5-40 mL: 0.3 g; then carrying out ultrasonic extraction for 5 to 45 min so as to obtain extract liquor; subjecting the obtained extract liquor to centrifugation, taking supernatant and introducing nitrogen into the supernatant so as to obtain concentrated supernatant; allowing the concentrated supernatant to pass through a filter membrane so as to obtain a to-be-detected solution; and carrying out quantitative analysis on the isothiazolinone compounds in the to-be-detected solution by using ultrahigh performance liquid chromatography-diode array. The method provided by the invention can carry out simultaneous analysis and detection on at least three isothiazolinone compounds in the water-based adhesive for cigarettes; the method is simple in treatment and fast in analysis speed; detection results are good in repeatability and high in sensitivity; and the method is applicable to determination of three isothiazolinone compounds in the water-based adhesive for cigarettes or in other complex matrixes.

The invention belongs to the field of software coordinative development, and discloses a public contribution combination request repeatability detecting method based on hybrid similarity. The method includes the steps of calculating the text similarity between a newly-submitted public contribution combination request and a historical public contribution combination request, calculating the variation similarity between the newly-submitted public contribution combination request and the historical public contribution, searching a public coordinative development platform for a historical repeatedcontribution data set, combining the text similarity and the variation similarity by means of a weight calculating method based on a greedy search strategy under the training of the data set to calculate the hybrid similarity of public contributions, and finally obtaining a list of historical public contribution combination requests the most probably repeated with a given public contribution combination request according to the value of the hybrid similarity. The public contribution repeatability can be detected in time, repeated artificial code inspection work is avoided, and the public contribution inspection efficiency is improved.

The invention belongs to the technical field of immune assay medical detection and particularly provides a PIVKA-II (protein induced by vitamin K absence-II) magnetic particle chemiluminescence immuneassay determination kit and a preparation method thereof. The kit mainly comprises (1) a PIVKA-II standard product; (2) anti-FITC (fluorescein isothiocyanate) monoclonal antibody coated magnetic particles; (3) an alkaline phosphatase marked PIVKA-II sample and fluorescein isothiocyanate marked PIVKA-II; (4) an alkaline phosphatase marked chemiluminescent substrate; (5) subpackage of the standardproduct, magnetic particle solution, mixed solution, sample dilution liquid, washing liquid, the chemiluminescent substrate and reaction tubes. By combination of the enzyme marking technique, the magnetic particle separation technique and the chemiluminescence detection technique, an approximately homogenous reaction system is provided, and simplicity, sensitivity, high specificity, wide linear range, high repeatability and the like are realized.

The invention belongs to the technical field of clinical in vitro diagnostic reagents and relates to a dry chemical reagent tablet for quantitatively measuring the concentration of total cholesterol and a preparation method of the dry chemical reagent tablet. The dry chemical reagent tablet comprises four laminations which are respectively a support layer, a color developing layer, a reagent layerand a diffusion layer distributed sequentially; and reagents in the reagent layer include a buffer system, a hydrophilic colloid, peroxidase, an activator, a surfactant and a stabilizer; and reagentsin the color developing layer include a chromogen and a hydrophilic colloid. Compared with the prior art, the dry chemical reagent tablet and the preparation method thereof of the invention have theadvantages of simple operation process, high stability, high good repeatability and high accuracy of detection results, quantitative detection, wide linear range, simple and convenient preparation process, easiness in operation, low risk and low pollution. According to the dry chemical reagent tablet and the preparation method of the dry chemical reagent tablet of the invention, the reagents do not need to be prepared, and samples can be directly added dropwise; the reagent tablet is dry and is free of moisture; the diffusion layer has a filtering function, so that hemolysis, bilirubin and ascorbic acid samples exert no significant interference on measurement results.

The invention discloses a preparation method of a hydrophobic polystyrene coated paper substrate. The preparation method comprises the following steps: completely dissolving a binder like a starch into deionized water at the normal temperature at the normal pressure, then adding a polystyrene particle, conducting ultrasonic dispersion to prepare an evenly dispersed coating material solution; thenevenly loading a polystyrene material on the surface of the paper substrate through a vacuum filtration coating method; finally fusing the polystyrene loaded on the surface of the paper substrate by means of high temperature baking, and effectively embedding the polystyrene in the surface of the paper substrate. According to the method provided by the invention, the hydrophobic polystyrene coatedpaper substrate with the surface tension of the water more than 95 degrees and the surface energy more than 45 mN/m is prepared, and the provided method has the characteristics of simple and convenient operation and good repeatability, the hydrophobic polystyrene coated paper substrate does not contain organic solvents, and the prepared paper substrate is good in flexibility and strong in hydrophobic property.

The invention relates to a real-time fluorescent PCR detection kit for bumblebee microsporidia and a detection method thereof, which are specially used for detecting bumblebee microsporidia. The kit comprises: (1) a positive standard; (2) negative control; (3) PCR reaction solution; (4) ExTaq enzyme; (5) sterile water. The invention has the following advantages: (1) good stability and specificity:detection of bumblebee microsporidia has high specificity, has no cross reaction with other microsporidia and bumblebee pathogens, and has good repeatability; (2) high sensitivity: the sensitivity can reach 20 copies/[mu] L; (3) easy and fast operation: the whole reaction can be completed within 2 hours. The kit can be used for detecting Nosema bombycis and differentiating and diagnosing the Nosema bombycis from other Nosema bombycis, and has important significance for early prevention and timely treatment of the disease. The kit can be used for detecting Nosema bombycis and differentiating and diagnosing the Nosema bombycis from other Nosema bombycis.

The invention provides full-automatic equipment and method for testing the helium diffusion coefficient of a reactor material. The equipment comprises a gas source control valve, a mass flow controller, a flow control valve, a low-vacuum-side vacuum gauge, a gas discharging control valve, a data acquisition card, a test pond control valve, a sample test pond, a sample to be tested, a high-vacuum-side vacuum gauge, a vacuum pump, a vacuum pump control valve, a helium detector, a computer, a helium gas source, a relay and pipelines; a test system comprises a data acquisition card and a computer; the computer is used for receiving, storing and analyzing data of the low-vacuum-side vacuum gauge, the high-vacuum-side vacuum gauge, the mass flow controller and the helium detector, and performing the process flow control on a system through controlling parts, so as to finally realize the automatic testing and result outputting. According to the equipment and the test method, the helium diffusion coefficient of the reactor material can be automatically tested and the problems of complexity in operation and poor test result repeatability in conventional test equipment are solved.

The invention provides a method for constructing an HPLC fingerprint of Pudilan Xiaoyan Tablets based on detection wavelength switching. The method absorbs Pudilan Xiaoyan Tablets, injects the test sample solution into a liquid chromatograph for high performance liquid chromatography analysis, and generates a standard fingerprint of the Pudilan Xiaoyan Tablets by using an average method through multi-point calibration and full spectrum peak matching, wherein the detection wavelength in the chromatographic conditions is 0-10min 323nm, 10-40min 280nm. The method provided by the invention provides a standard fingerprint of Pudilan Xiaoyan tablets and carries out quality analysis and evaluation through the similarity evaluation of the listed samples, has the advantages of simple operation highrepeatability, precision and stability, and can comprehensively and accurately evaluate the process rationality and product uniformity of the Pudilan Xiaoyan Tablets.

The invention provides a test card capable of avoiding the influence of wet gas on the accuracy in a test process and application of the test card. The test card comprises a test strip and a card box,wherein the card box is provided with a detection window; a dampproof thin layer for isolating environmental wet gas covers the detection window; the dampproof thin layer is a lightproof material orlight-transmitting material thin layer. The application is that the test card is applied to immunochromatography, and particularly to detection of a nitrotyrosine indicator. The test card can avoid the influence of environmental humidity in the detection process, so that the stability and the consistency of a test card detection result are improved; meanwhile, the application range of the test card to the environment is enlarged, so that the test card is particularly suitable for measurement under a high-humidity environment; when the test card is applied to detection of nitrotyrosine, quick and quantitative detection of the nitrotyrosine can be realized; moreover, a requirement on assorted equipment is low, and the detection process can be greatly simplified; the test card has the characteristics of wide linear range, high accuracy, high repetitiveness and the like.

The invention relates to a characterization method for the strengthening phase of a ferromagnetic alloy block and/or film. An optimum scheme includes: separating a strengthening phase of nano to micron size in a ferromagnetic alloy from an alloy matrix to obtain an electrolyte solution containing the strengthening phase; then conducting dilution by anhydrous ethanol and ultrasonic dispersion, thendripping the obtained solution to an ultra-thin carbon support film, and performing drying to obtain an electron microscope sample; and then using an electron microscope for structural observation and characterization. The method provided by the invention can characterize the morphology and structure of the strengthening phase less than 0.5micron in the ferromagnetic alloy, especially the strengthening phase less than 50nm in size. The strengthening phase separated by the method provided by the invention retains the original structure of the strengthening phase, and the method has the advantages of simplicity and high efficiency, easily available electrolytic conditions, simple operation and strong repeatability, and can be used for analysis and characterization of strengthening phases invarious ferromagnetic materials. The method provided by the invention effectively avoids the adverse effects of ferromagnetic alloy matrixes on electron microscope equipment and the detection process, and realizes the TEM/HRTEM structural characterization of the ferromagnetic alloy strengthening phase.

The invention belongs to the technical field of detection methods and particularly relates to a method for detecting the content of blue dyestuff in a textile and a leather product. The method comprises the steps ofby taking the textile or the leather product as a sample, and cutting the sample into pieces; weighing a certain amount of sample into a reaction pipe, then adding a polar organic solvent into the reaction pipe, enabling the mass ratio of the sample tothe polar organic solvent to be 1 to (5-50), and then putting the reaction pipe into an ultrasonic cleaner to carry out ultrasonic extraction; after the extraction is finished, taking out the reaction pipe, cooling, fully shaking, standing, and filtering supernatant liquor into a sample bottle; anddetectingand analyzingthe blue dyestuff in sample solution byadopting a high-performance liquid chromatograph with a diode array detector. Compared with the prior art, the method has the beneficial effects that not only can the qualitativeness be accurate, but also the quantitativeness can be accurate. The method has various advantages of low detection limit, good repeatability, low cost, convenience and fastness and the like, and the requirements of the European-union instructions and regulations for managing and controlling the blue dyestuff can be completely met.