How to Standard product?

Q: How to Standard product?

Enzyme-linked immunologic detection method of bisphenol A,Matrix dispersion solid phase extraction method for detection of plasticizer in animal and vegetable oil samples,PIVKA-II (protein induced by vitamin K absence-II) magnetic particle chemiluminescence immune assay determination kit and preparation method thereof,Detection method for content of chlorogenic acid in stauntonia chinensis DC

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Definition of Standardized Product: A standardized product is a product that regardless of what company furnishes the good, consumers regard the product furnished by all the companies as identical. Examples of standardized products include agricultural products (such as grain and milk), most mined minerals, and fish.

Enzyme-linked immunologic detection method of bisphenol A

InactiveCN101413945AHigh selectivityIncreased sensitivityMaterial analysis by observing effect on chemical indicatorElisa methodImmuno detection

An enzyme-linked immunoassay method of bisphenol A belongs to the technical field of immunodetection. The enzyme-linked immunosorbent assay method utilizes the immunization of a synthetic bisphenol A immunogen to obtain a polyclonal antibody, takes the bisphenol A as a standard product, takes a conjugate of a hapten of diphenolic acid and OVA as a coating antigen and establishes the indirect competitive enzyme-linked immunosorbent assay method of the bisphenol A. The enzyme-linked immunosorbent assay method establishes the indirect competitive ELISA method of the bisphenol A and provides a rapid and high-efficient detection method for detecting the residual bisphenol A, as the method adopts the polyclonal antibody, the cost is lower, and the stability and the repeatability are good. The sensitivity is 0.1ng / ml, and the linear range is 0-100ng / ml. The high specificity and the affinity of the immune reaction lead the ELISA to have very high selectivity and sensitivity.

Owner:JIANGNAN UNIV

Matrix dispersion solid phase extraction method for detection of plasticizer in animal and vegetable oil samples

InactiveCN102620978ASimplify the experimental stepsExperimental steps save timePreparing sample for investigationVegetable oilGas chromatography–mass spectrometry

The invention relates to a matrix dispersion solid phase extraction method for detection of plasticizer in animal and vegetable oil samples. The method comprises the following steps: (1) packing an extraction glass centrifuge tube: weighing 0.1-5g of solid phase extraction filler in a glass centrifuge tube with a stopper to obtain an extraction glass centrifuge tube; (2) weighing, dissolving and extracting: weighing 0.1-5g of an animal or vegetable oil sample, dissolving with 1-10ml acetonitrile saturated n-hexane, extracting with 1-10ml n-hexane saturated acetonitrile twice, centrifuging, combining acetonitrile layers as to-be-purified liquid; and (3) purifying: transferring the to-be-purified liquid into the extraction glass centrifuge tube, sufficiently shaking, centrifuging at a rotation speed of 1,000-10,000 r / min, collecting supernatant, analyzing with GC-MS (gas chromatography-mass spectrometry), establishing a standard curve with a standard sample, and carrying out quantitative and qualitative analysis. The method provided by the invention is especially suitable for rapid detection of phthalates in animal and vegetable oil samples.

Owner:TIANJIN CHUNFA BIO TECH GRP

PIVKA-II (protein induced by vitamin K absence-II) magnetic particle chemiluminescence immune assay determination kit and preparation method thereof

PendingCN109425740AHigh sensitivityEasy to operateMaterial analysisBlood plasmaFluorescein isothiocyanate

The invention belongs to the technical field of immune assay medical detection and particularly provides a PIVKA-II (protein induced by vitamin K absence-II) magnetic particle chemiluminescence immuneassay determination kit and a preparation method thereof. The kit mainly comprises (1) a PIVKA-II standard product; (2) anti-FITC (fluorescein isothiocyanate) monoclonal antibody coated magnetic particles; (3) an alkaline phosphatase marked PIVKA-II sample and fluorescein isothiocyanate marked PIVKA-II; (4) an alkaline phosphatase marked chemiluminescent substrate; (5) subpackage of the standardproduct, magnetic particle solution, mixed solution, sample dilution liquid, washing liquid, the chemiluminescent substrate and reaction tubes. By combination of the enzyme marking technique, the magnetic particle separation technique and the chemiluminescence detection technique, an approximately homogenous reaction system is provided, and simplicity, sensitivity, high specificity, wide linear range, high repeatability and the like are realized.

PIVKA-II (protein induced by vitamin K absence-II) magnetic particle chemiluminescence immune assay determination kit and preparation method thereof

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Owner:江苏麦得科生物科技有限公司

Detection method for content of chlorogenic acid in stauntonia chinensis DC

InactiveCN110133138AGood linear relationshipAvoid recycling operationsComponent separationChlorogenic acidPhosphate

The invention discloses a detection method for the content of chlorogenic acid in stauntonia chinensis DC. The detection method comprises the following steps that: 1) smashing a stauntonia chinensis DC product into powder, adding methyl alcohol, carrying out ultrasonic extraction for 30min, and filtering to obtain a sample to be detected; 2) weighing a chlorogenic acid standard product, then configuring 1mg / ml of chlorogenic acid reference substance stock solution by the methyl alcohol; 3) measuring by high performance liquid chromatography for measurement, wherein a stationary phase is an Accucore aQ chromatographic column, a moving phase A is phosphate buffered solution, a moving phase B is acetonitrile, the volume ratio of A to B is 85:15, a flow rate is 0.6mL / min, detection wavelengthis 325nm, a column temperature is 30DEG CC, and a sample feeding amount is 2[Mu]L; and 4) taking the peak area of the reference substance stock solution is a vertical coordinate, taking the concentration of the reference substance stock solution as an abscissa, drawing the standard curve of the chlorogenic acid, and calculating the content of the chlorogenic acid in the stauntonia chinensis DC ina sample to be detected. By use of the above method, the stauntonia chinensis DC product which contains the chlorogenic acid under low concentration can be effectively detected, detection time is short, and accuracy is high.

Owner:GUIZHOU TIANLOU BIOLOGICAL DEV CO LTD

4 results about "Standard product" patented technology

Enzyme-linked immunologic detection method of bisphenol A

Matrix dispersion solid phase extraction method for detection of plasticizer in animal and vegetable oil samples

PIVKA-II (protein induced by vitamin K absence-II) magnetic particle chemiluminescence immune assay determination kit and preparation method thereof

Detection method for content of chlorogenic acid in stauntonia chinensis DC

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