7 results about "Immune reaction" patented technology

Isolated and purified peptides and variants thereof, useful to prevent or treat antibody-mediated diseases, or indications caused by an undesirable antibody response to a given antigen, are provided. Also provided are peptides and methods useful to prevent or treat indications associated with the use of viral vectors in gene replacement therapy. Further, a method to inhibit or prevent aberrant immune responses to exogenous, non-infectious antigen is provided.

The invention provides preparation and application of a ractopamine sample pretreatment kit based on immunomagnetic beads. The kit mainly comprises a sample compartment, a reagent compartment, a cleaning compartment and an elution compartment. The kit expands the binding surface area and makes immune reaction more thorough enrichment of the immunomagnetic beads and full diffusion of the immunomagnetic beads in liquid, and can be widely applied to enrichment and separation of ractopamine in animal tissues, aquatic products, meat products and the like, which can improve the sensitivity of detection and avoid the phenomenon of missed detection; at the same time, a large amount of organic solvents used are reduced.

The present invention relates to an immunoreaction measuring method for measuring an antigen or antibody contained as a subject substance in a sample, wherein the sample was mixed with at least one compound selected from the group consisting of dicarboxylic acids having a hydroxyl group, dicarboxylic acids having a double bond, straight-chain dicarboxylic acids expressed by the chemical formula (1): HOOC(CH₂)_nCOOH (n is an integer), and the salts of these dicarboxylic acids, and an antibody or antigen as a specifically binding substance capable of specifically binding to the subject substance, to obtain an acidic reaction solution, and then an antigen-antibody complex, generated by an antigen-antibody reaction of the subject substance with the specifically binding substance in the reaction solution was detected. This makes it possible to improve a measurement value and to relax a limitation of a measurement range due to a zone phenomenon that occurs in an antigen-excess region.

The invention provides an amphiphilic alpha-helical polypeptide for inhibiting PD-L1 (programmed death-ligand 1) and application of the amphiphilic alpha-helical polypeptide. By investigating the in-vitro PD-1/PD-L1 protein binding inhibition effect, the in-vivo and in-vitro anti-tumor effect and the in-vivo immune activation effect of the polypeptide, it is verified that the polypeptide can block a PD-1/PD-L1 signal channel and activate the immune response of T cells and has the anti-tumor effect. The polypeptide disclosed by the invention can be prepared by adopting a solid-phase synthesis method, and compared with a monoclonal antibody and a small molecule compound, the polypeptide disclosed by the invention solves the problems of insufficient immune-related toxicity, pharmacokinetics and tumor permeation, high cost burden of manufacturers and consumers and the like.

The invention provides a GD2 chimeric antigen receptor and application thereof. A humanized scFv antibody of GD2 has the activity of being combined with a GD2 antigen; the amino acid sequence of the humanized scFv antibody of the GD2 has 80% or more of identity with the amino acid sequence shown in SEQ ID NO.1. The invention further discloses a preparation method of the humanized scFv antibody of the GD2. The invention also provides a GD2 chimeric antigen receptor and a chimeric antigen receptor T cell for expressing the GD2 chimeric antigen receptor. The humanized scFv antibody of the GD2 disclosed by the invention has better biological activity and compatibility; after the GD2 chimeric antigen receptor is combined with GD2, a better reaction effect is achieved, the immunoreaction is stronger, and the GD2 chimeric antigen receptor has a better long-term effect; the chimeric antigen receptor T cell has higher safety and durability, and is extremely high in application value.