7 results about "Linear range" patented technology

The invention provides a preparation method for an engineering strain of Escherichia coli used for detecting the heavy metal copper and establishment of a method for detecting copper in a water body by using the engineering strain of Escherichia coli. The engineering strain provided by the invention has the technical characteristics of specificity, sensitivity, rapidness, cheap price, stability and capacity of quantitative determination of the concentration and bio-availability of copper in a water body. The engineering strain has a wide detection linear range and high sensitivity in detection of copper ions, wherein the detection linear range is 0.39 to 94 [mu]M and lowest detection limit is 0.25 [mu]M; the engineering strain is applicable to both quantitative determination of copper in water bodies like various sewage, domestic drinking water, acidic mine wastewater and electroplating wastewater and to assessment of biotoxicity of treated or restored copper-contaminated water bodies; so the engineering strain and physical and chemical analytical methods like atomic absorption spectrometry can realize complementary analysis of bio-availability and total amount of copper in water bodies, thereby providing bases for objective evaluation of the biotoxicity of the heavy metal copper.

The invention belongs to the technical field of immune assay medical detection and particularly provides a PIVKA-II (protein induced by vitamin K absence-II) magnetic particle chemiluminescence immuneassay determination kit and a preparation method thereof. The kit mainly comprises (1) a PIVKA-II standard product; (2) anti-FITC (fluorescein isothiocyanate) monoclonal antibody coated magnetic particles; (3) an alkaline phosphatase marked PIVKA-II sample and fluorescein isothiocyanate marked PIVKA-II; (4) an alkaline phosphatase marked chemiluminescent substrate; (5) subpackage of the standardproduct, magnetic particle solution, mixed solution, sample dilution liquid, washing liquid, the chemiluminescent substrate and reaction tubes. By combination of the enzyme marking technique, the magnetic particle separation technique and the chemiluminescence detection technique, an approximately homogenous reaction system is provided, and simplicity, sensitivity, high specificity, wide linear range, high repeatability and the like are realized.

The invention belongs to the technical field of clinical in vitro diagnostic reagents and relates to a dry chemical reagent tablet for quantitatively measuring the concentration of total cholesterol and a preparation method of the dry chemical reagent tablet. The dry chemical reagent tablet comprises four laminations which are respectively a support layer, a color developing layer, a reagent layerand a diffusion layer distributed sequentially; and reagents in the reagent layer include a buffer system, a hydrophilic colloid, peroxidase, an activator, a surfactant and a stabilizer; and reagentsin the color developing layer include a chromogen and a hydrophilic colloid. Compared with the prior art, the dry chemical reagent tablet and the preparation method thereof of the invention have theadvantages of simple operation process, high stability, high good repeatability and high accuracy of detection results, quantitative detection, wide linear range, simple and convenient preparation process, easiness in operation, low risk and low pollution. According to the dry chemical reagent tablet and the preparation method of the dry chemical reagent tablet of the invention, the reagents do not need to be prepared, and samples can be directly added dropwise; the reagent tablet is dry and is free of moisture; the diffusion layer has a filtering function, so that hemolysis, bilirubin and ascorbic acid samples exert no significant interference on measurement results.

The invention disclose a tyrosine-sensing electrochemical working electrode, in particular relates to a tyrosine-sensing electrochemical working electrode based on a molybdovanadophosphoric heterpolyacid-doped carbon nanotube / chitosan-copper chelate, belongs to the technical field of electrochemical sensors, and aims at solving the problems of narrow linear ranges, low sensitivity and high preparation cost of tyrosine detection non-enzyme electrochemical sensors in the prior art. The tyrosine-sensing electrochemical working electrode comprises an ITO electrode and a PEI / [P2Mo16V2-MWCNTs /CTS-Cu]n film externally coating the ITO electrode, a molybdovanadophosphoric heterpoly acid-doped carbon nanotube / chitosan-copper chelate layer is a cycle, the number of the cycles is n, and n = 1-10. The electrochemical sensor constructed based on the working electrode has good tyrosine sensing property, the linear range is 4.99 * 10<-8>M-6.88 * 10<-5>M (M:mol / L), and the sensitivity is 0.206 mu M /mu A.

The invention provides a test card capable of avoiding the influence of wet gas on the accuracy in a test process and application of the test card. The test card comprises a test strip and a card box,wherein the card box is provided with a detection window; a dampproof thin layer for isolating environmental wet gas covers the detection window; the dampproof thin layer is a lightproof material orlight-transmitting material thin layer. The application is that the test card is applied to immunochromatography, and particularly to detection of a nitrotyrosine indicator. The test card can avoid the influence of environmental humidity in the detection process, so that the stability and the consistency of a test card detection result are improved; meanwhile, the application range of the test card to the environment is enlarged, so that the test card is particularly suitable for measurement under a high-humidity environment; when the test card is applied to detection of nitrotyrosine, quick and quantitative detection of the nitrotyrosine can be realized; moreover, a requirement on assorted equipment is low, and the detection process can be greatly simplified; the test card has the characteristics of wide linear range, high accuracy, high repetitiveness and the like.