The invention provides a method for detecting MFG-E8. The method includes 1), preparing detection samples and standard substances; 2),
coating and adsorbing anti-human MFG-E8
protein mouse monoclonalantibodies in coated plates; 3), allowing the detection samples and the standard substances to be in contact with the coated anti-human MFG-E8
protein mouse
monoclonal antibodies, adding anti-human MFG-E8
protein sheep
polyclonal antibodies, anti-sheep IgG rabbit antibodies labeled by
horseradish peroxidase and
luminescence substrates into the detection samples and the
standard samples, allowing the detection samples and the standard substances to be in contact with the anti-human MFG-E8 protein sheep
polyclonal antibodies, the anti-sheep IgG rabbit antibodies labeled by the
horseradish peroxidase and the
luminescence substrates, carrying out light protective reaction, measuring
chemiluminescence values and computing the content of the MFG-E8 in the detection samples. The method has the advantages that the method is good in precision, low in
detection limit, high in sensitivity and wide in
linear range, clinical application requirements can be met, and the like.