92 results about "Enzyme" patented technology

The present invention relates to a novel enzymatic bleaching system, including at least one oxidase and at least one perhydrolase, body care agents, hair shampoos, hair care agents, mouth-, tooth- or denture care products, cosmetics, therapeutics, textile detergents, cleaning compositions, rinsing agents, textile detergents for automatic washing machines, hand detergents, hand dishwashing agents, automatic dishwasher agents, disinfectants and agents for bleaching or disinfecting filter media, textiles, hides, paper, skins or leather, comprising the novel enzymatic bleaching system, as well as uses of the novel enzymatic bleaching system and this composition.

The invention discloses a seafood flavoring and process for preparation. At present, seafood such as little fish, low-value fish and shellfish and the like can not be fully exploited and utilized, and the processing rate is lower than 30%, some of the seafood is directly thrown into sea areas as feedingstuff, which causes resource waste and sea-area pollution. The invention utilizes the method of biological enzymolysis to extract biological proteins and to produce various seafood flavorings by mixing the proteins with relative raw materials, which increases the utilization value of little fish, low-value fish and shellfish. The invention has the advantages of short enzymolysis time, high protein extract rate which can reach 60%, yield over 9.9%, moderate reaction condition, and lower production cost, which is suitable for industrial production.

The invention relates to a method of treatment of resistance to platelet inhibitors, i.e. a method to overcome resistance of treatment with platelet inhibitors, said method comprising administering a therapeutically effective amount of dipyridamole in combination with a platelet inhibitor and, optionally, in combination with a third antithrombotic component such as direct thrombin inhibitors, factor Xa inhibitors, combined thrombin/factor Xa inhibitors, heparin, low molecular weight heparin, argatroban, bivalrudin, hirulog or polyglycans to a patient in need thereof. The invention further relates to the use of dipyridamole for the manufacture of a pharmaceutical composition for treatment of resistance to platelet inhibitors. The invention also relates to a method to diagnose resistance to treatment with platelet inhibitors, said method comprising measurement of the density of binding of Annexin V on platelets.

The invention provides methods and compositions for detecting a change in a nucleic acid polymerase conformation involving contacting a nucleic acid polymerase non-covalently attached to a single walled carbon nanotube (SWNT) with a first nucleotide or first nucleotide analog and a template and detecting the conformationally changed nucleic acid polymerase by measuring a first electrical conductance change in the SWNT between the nucleic acid polymerase and the conformationally changed nucleic acid polymerase. The method is useful for sequencing of polynucleotides.

A composition for treatment of bacterial infections of the eye is disclosed which comprises a lytic enzyme composition specific for the infecting bacteria, and a carrier for delivering said lytic enzyme. The carrier for delivering at least one lytic enzyme to the eye may be but is not limited to the use of an isotonic solution.

The invention discloses a pressure balance system of an automatic ELISA (Enzyme-Linked Immunosorbent Assay) plate washer and the ELISA plate washer. The ELISA plate washer comprises a cleaning head, a vacuum pump and a plurality of lotion bottles for providing lotion for a lotion needle of the cleaning head; and the pressure balance system comprises a first multi-path joint, a main exhaust pipe and at least one auxiliary exhaust pipe, wherein the first multi-path joint is connected with the vacuum pump and the lotion bottles and used for balancing the pressure suffered by the lotion bottles, the main exhaust pipe and the at least one auxiliary exhaust pipe are respectively connected with the first multi-path joint and used for discharging air outside, and a switch is arranged on the auxiliary exhaust pipe and connected with a control circuit for controlling the on and off of the switch. According to the invention, the pressure balance system is arranged in the ELISA plate washer, so that the pressure of a pipeline in the ELISA plate washer can be adjusted automatically, the pressure generated by the vacuum pump can be matched with the flow of the lotion needle on the cleaning head automatically, the flowing rate of the lotion distributed for the lotion needle by the cleaning head can be kept consistent under any state, so that when a large plate washer is used for few pore plates, the damage of an instrument as part of washing channel is closed and excessive overflowing liquid overflows a tray and enters the machine can be prevented.

The invention belongs to the technical field of immunological analysis, in particular to an ELISA method for detecting doxycycline (DOX) remnant and a kit. The method mainly comprises the preparation of immunogen, coatingen and antibody, the pretreatment of the sample and the establishment of the ELISA detection method. The kit of the invention mainly comprises anti DOX specific antibody, a DOX standard substance, and an elisa plate coated with DOX-OVA conjugate. The kit and the method of the invention are characterized by simplicity, convenience, high speed, sensitivity, accuracy and the like and are suitable for detecting DOX remnant in animal edibility tissue.

The invention discloses a chromium-based functional nucleic acid sensor and an application thereof in the technical field of metal ion detection. The sensor comprises a molecule recognition element, asignal amplification element and a signal conversion element, wherein the molecule recognition element comprises chromium ion deoxyribozyme consisting of a substrate chain and an enzyme chain; the signal amplification element comprises an isothermal amplification system and hemin, and the isothermal amplification system comprises an amplification template; the signal conversion element comprisesa color developing agent. The sensor is based on the chromium ion deoxyribozyme, an isothermal index amplification reaction and a G-quadruplex liquid phase sensing technology, is convenient, quick, high in sensitivity, high in specificity and resistant to high salinity in chromium ion detection and can be used in field detection of chromium ions in the environment.

The invention relates to a preparation method of cyclosporin A haptin and an enzymelinked immunosorbent quantitative detection kit of the cyclosporin A, belonging to medical detection reagents. The invention mainly provides a preparation method of cyclosporin A haptin and the cyclosporin A haptin prepared by using the preparation method. The enzymelinked immunosorbent quantitative detection kit of cyclosporin A, adopting the cyclosporin A haptin, can be made into a plate-type ELISA (enzyme-linked immunosorbent assay) plate so as to adapt to popular detection equipment in China and can ensure high detection sensitivity, good stability and accurate result.

The invention discloses a high-salinity-resistant nucleic acid sensor for copper and application thereof. The sensor comprises a molecular recognition element, a signal multiplication element and a signal conversion element, wherein the molecular recognition element comprises copper ion deoxyribozyme; the copper ion deoxyribozyme consists of a substrate chain and an enzyme chain; the signal multiplication element comprises an isothermal amplification system; the isothermal amplification system comprises an amplification template; the signal conversion element comprises hemin and a color developing agent. The sensor provided by the invention is used for specifically recognizing a copper ion; the primary enlargement and conversion of a signal are carried out through an isothermal index multiplication reaction; a G-four-chain body structure with activity is formed under the induction of the hemin; the color developing agent is catalyzed to develop a color, thus, secondary multiplication and conversion are generated; the signal is converted into a visual signal, and the qualitative and quantitative detection in a high-salinity environment can be carried out.

The invention belongs to the field of protein analysis, and relates to a new method for enrichment of a glycosylated peptide through a magnetic nanometer material modified by aminoxy, in particular to a method for solid-phase enrichment and mass spectrographic analysis of a glycosylated peptide fragment. The method comprises the following steps: firstly, using sodium periodate NaIO4 to oxidize a hydroxyl group on a glycopeptide to change the hydroxyl group to an aldehyde group; secondly, placing the magnetic nanometer material Fe3O4@ONH2 modified by aminoxy to an oxidized glycopeptide solution, and fixing the glycopeptide to the magnetic nanometer material through reaction of an aminoxy group and the aldehyde group obtained after a sugar chain in the glycopeptides is oxidized; thirdly, cleaning to remove non-glycopeptide which does not react with the nanometer material; finally, dissociating the captured glycopeptide from the material by use of sugar chain enzyme, and realizing mass spectrographic analysis of the glycosylated peptide. The method is simple in step, convenient to operate, high in speed and efficient, and can realize highly-sensitive and highly-selective mass spectrometric analysis of the glycosylated peptide.

The invention discloses a chemiluminescence immunoassay kit for Seneca valley virus nonstructural protein 3ABC antibody detection. The kit comprises a Seneca valley virus nonstructural protein 3ABC antigen coated chemiluminescence immunoassay plate, positive control serum, negative control serum, an enzyme-labeled antibody, a sample diluent, a chemiluminescence substrate solution, a luminescence enhancing agent and concentrated washing liquid, wherein the amino acid sequence of the Seneca valley virus nonstructural protein 3ABC is shown as SEQ ID NO.2. The kit provided by the invention uses nonstructural protein 3ABC antigen expressed by a prokaryotic expression system for coating a reaction plate; the antigen consumption is low; whether the Seneca valley virus exists in the pig serum or not can be efficiently detected; in addition, reaction with the swine vesicular disease viruses, hog cholera viruses and porcine circovirus type 2 cannot occur. An enzymatic chemiluminescence reactionsystem is used for judging the result; the detection sensitivity is improved. The kit provided by the invention has the advantages that the specificity is high; sensitivity and high efficiency are realized; good market prospects are realized.

The invention provides a preparation method of monoclonal antibodies for IC (nfectious coryza) of chickens. According to the technical scheme, hybridoma cell strains secreting the monoclonal antibodies against avibacterium paragallinarum are prepared from B type strains, the secreted antibodies are only targeted at the B type strains and do not have reactions with A type and C type avibacterium paragallinarum, and accordingly, the antibodies are only targeted at B type serotype diseases. Besides, an ELISA (enzyme-linked immunosorbent assay) method and an immune colloidal gold method established according to the characteristics of the monoclonal antibodies have good specificity and sensitivity and can be used for clinical detection. Besides, the monoclonal antibodies prepared with the method are high in titer, better in specificity and especially applicable to further preparation of B type avibacterium paragallinarum antigen diagnostic kits or B type avibacterium paragallinarum antigen diagnostic colloidal gold test paper. Prominent technical effects are realized through innovative technical improvements, and the preparation method is lower in cost and easy to implement and has prominent popularization prospect.

The present invention discloses a photoelectrochemical biosensor comprising an electrolytic cell, electrolyte provided in the electrolytic cell, an organic electrochemical transistor provided in the electrolytic cell, and a gate electrode provided in the electrolytic cell, wherein the organic electrochemical transistor comprises a substrate, a source electrode and a drain electrode disposed on the substrate, and an organic semiconductor thin film layer coating the substrate and connected with the source electrode and the drain electrode; the gate electrode is modified with a photoelectric active semiconductor material as a sensitive functional layer of the sensor. The photoelectrochemical biosensor of the invention has extremely high sensitivity, the structure is simple and the device size is small, and the problem that the photoelectrochemical biosensor is not easy to miniaturize is solved. The photoelectrochemical biosensor of the invention has universality in the field of biological detection, and can be widely applied in the biosensor field such as enzyme biosensor, cell biosensor and the like, besides applied to DNA sensor and immune sensor.

The invention belongs to the technical field of food processing, and provides bran-free grain noodles. The bran-free grain noodles are made from the following raw materials in parts by weight of 1.0-1.2 parts of bran-free cereal powder, 0.05-0.1 part of mucedin-free plant protein powder, 0.01-0.02 part of yeast powder, 0.01-0.05 part of TG enzymes, 0.01-0.05 part of lactobacillus plantarum powder,0.01-0.03 part of table salt, 0.01-0.02 part of a flour improver and 0.5-0.6 part of water. The raw materials are in cooperation in proportion, so that the viscoelastic properties of bran-free graindough can be increased, and the chewiness and the tenacity of the dough can be increased; and therefore, the finally-made noodles are low in breaking rate, rich in nutrients, good in mouth feel and good in flavor.

The invention provides a biological treatment method of beer wastewater, and the method utilizes the activity of biological enzyme and metabolism of hydrolysis acidate bacteria to treat harmful substances in the beer wastewater. The method has the advantages of simple operation, short treatment time, having no secondary pollutant in the treatment process, safety and environmental protection.

The invention discloses a heat-resistant formate dehydrogenase gene, its coded polypeptide and a preparation method. Based on Bacillussp., the invention clones and isolates the heat-resistant formate dehydrogenase gene, which is useful for the preparation of It is useful to obtain heat-resistant formate dehydrogenase transgenic microorganisms, animals and plants, and recover the enzyme encoded by the gene. In addition, the present invention also provides the amino acid sequence and functional equivalent of the polypeptide having heat-resistant formate dehydrogenase activity. At the same time, the invention also provides a method for preparing, separating and purifying the polypeptide with heat-resistant formate dehydrogenase activity.

The invention aims to provide a method for improving euryops pectinatus oil aroma quality and application thereof. The method includes: subjecting euryops pectinatus to low temperature superfine comminution, then adding a certain amount of acid, an oxidant and enzyme and mixing the substances evenly in an aqueous solution, conducting incubation fermentation for a certain period of time under a synergistic effect, and carrying out steam distillation to extract volatile oil. The method improves the extraction rate and aroma quality of euryops pectinatus oil, the euryops pectinatus oil has composite, natural and rich aroma, besides chrysanthemum characteristic aroma, also has flower fragrance, fruit sweet aroma, slightly cool sense feel and other natural smell, the irritation is reduced, andthe aroma quality is improved. The prepared euryops pectinatus oil can be applied in cigarette formula cut tobacco, reconstituted tobacco, and cut stems, the prepared cigarette has clear aroma, chrysanthemum characteristic note, rich and pure aroma, and clean aftertaste, and overcomes the defects of too strong chrysanthemum characteristic aroma, increased irritation, little miscellaneous qi, and slightly bitter aftertaste.

Disclosed is a process for producing the L-form of an amino acid enzymatically from an enantiomeric mixture of the amino acid. The process includes the step of contacting a transformant or a treated product thereof with an enantiomeric mixture of the amino acid. The transformant includes a single host and one or more recombinant vectors introduced thereinto, in which the one or more recombinant vectors include one or more expression vectors and nucleotide sequences integrated thereinto respectively, and the nucleotide sequences are a nucleotide sequence encoding an enzyme capable of converting the D-form of an amino acid into a keto acid and a nucleotide sequence encoding an enzyme capable of converting a keto acid into the L-form of an amino acid. According to this process, a reaction for converting the D-form of an amino acid into a keto acid and a reaction for converting the keto acid into the L-form of the amino acid can be conducted in a single step. Disclosed also is a transformant capable of converting into the L-form of an amino acid as accumulated in a high concentration. The L-form of an amino acid can be efficiently produced from an inexpensive starting material by using the transformant.

The invention discloses an antibacterial whitening mouthwash and a preparation method thereof. The antibacterial whitening mouthwash comprises an antibacterial component and a mouthwash matrix component, wherein the antibacterial component comprises 0.05 to 0.5 part of lysozyme, 0.01 to 0.5 part of lauryl arginine ethyl ester hydrochloride, 0.01 to 1 part of dextran anhydrase and 1 to 10 parts ofabelmoschus manihot extract. A substrate component of the mouthwash comprises one or more of an emulsifier, a moisturize, a flavoring, an acid-base conditioner and an essence, and the balance is deionized water. Lysozyme and lauryl arginine ethyl ester hydrochloride are dissolved in a citric acid solution and uniformly stirred; the prepared ingredients are mixed with the emulsifier, moisturizer, flavoring and essence evenly, pH is adjusted, and abelmoschus manihot extract and dextran enzyme are added. The invention makes the synergistic effect between the natural antibacterial agent and the chemical antibacterial agent exist, and the mouthwash produced by the invention has high safety and good antibacterial activity, and can effectively prevent and control the occurrence of dental plaque and oral inflammation.

The invention relates to a method for efficiently amplifying subgroup J avian leukosis virus (ALV). According to the method, chicken hepatocytes can be used for efficiently amplifying the subgroup J ALV, the characteristic of high titer virus can be produced earlier by using the chicken hepatocytes, the separation and detection time of the subgroup J ALV can be shortened, the pathogen can be detected earlier, and the sensitivity of a subgroup J ALV detection method is improved. When subgroup J ALV infection MOI is 0.001 hour, the effect of enzyme-linked immunosorbent assay (ELISA) on the chickhepatocytes infected for 3 days is comparable to the effect of ELISA on DF-1 cells infected for 7 days. The efficient amplification method established by the invention accelerates the purification process of the subgroup J ALV, thus shortening the time and reducing cost; the method has very good application value in the purification detection of the subgroup J ALV.