114 results about "Microorganism" patented technology

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

The invention relates to a method for producing an amino acid tobacco-specific foliar fertilizer by using abandoned fur. The method comprises the following steps of: degreasing, drying and crushing various abandoned animal hair in slaughterhouses, hair in barber shops and various abandoned animal leather which are used as main raw materials; carrying out microbial fermentation, mainly Freund crack mould or other keratin decomposing bacteria fermentation; then decomposing and filtering to prepare a crude amino acid solution; and chelating the following components in parts by weight: 30-50 parts of crude amino acid solution, 15-18 parts of potassium sulfate, 15-25 parts of ammonium sulfate, 15-18 parts of potassium dihydrogen phosphate, 3-6 parts of humic acid potassium, 1.3-2 parts of trace elements, 0.3-0.5 part of surfactant, 0.5-1 part of chelating agent and 0.15-0.2 part of plant growth regulating agent. The tobacco-specific foliar fertilizer has the effects of promoting growth of tobacco roots, enhancing the absorption and utilization of tobacco on soil and the fertilizer, promoting lengthening, widening and thickening of tobacco, enhancing the ability of disease resistance, and improving the quality and yield of tobacco leaves.

The invention discloses a solid microbial agent used for remedying the soil contaminated by petroleum, belonging to the technical field of bioremediation of soil contaminants. First-class seed liquid of acinetobacter radioresistens and rhodococcus erythropolis are mixed in equal parts after two steps of fermentation in liquid and solid states and cultivation to obtain the solid microbial agent, wherein, the number of effective viable bacteria of acinetobacter radioresistens and rhodococcus erythropolis is 10 to 10 CFU/g. The solid microbial agent in the invention has the advantages of low cost, high number of viable bacteria; the invention further has the advantages that micro-ecological environment improvement of the soil can be improved and secondary contamination cannot be caused to the environment, thus applicable to the bioremediation of the soil which is contaminated by petroleum and petroleum products.

The invention relates to an edible mushroom, spent mushroom substrate and probiotics-containing nutrient solution for livestock and poultry and a preparation method thereof. The preparation method comprises the following steps of: preparing leach liquor by using waste spent mushroom substrates as raw materials, extracting a plurality of active ingredients from the spent mushroom substrates, and then fermenting and preparing the nutrient solution containing a plurality of microorganisms beneficial for the growth of the livestock and poultry by using the leach liquor as a culture medium. The prepared nutrient solution is brown in color and tart, slightly has the incenses of edible mushrooms, and contains abundant probiotics groups so as to be beneficial for enhancing the appetite of the livestock and poultry and promoting the absorption of nourishment and the growth of the livestock and poultry. Compared with the solid fermentation of the conventional preparation method of spent mushroom substrate-containing feeds, due to the adoption of the main production method of liquid fermentation, the preparation method of the nutrient solution has the advantages of environmental protection, energy saving and convenient operation; and the obtained product is liquid so as to have the advantages of convenient application and favorable palatability for the livestock and poultry.

A method of selection for type II methanotrophs is provided that includes enriching a microbial feedstock using a non-sterile bioreactor with a methane source and a nitrogen source, where the microbial feedstock includes a mixture of Type I and Type II methanotrophic cells, where an inhibited growth of the Type I methanotrophic cells and an enhanced growth of the Type II methanotrophic cells forms. The method further includes exposing intermittently the enriched microbial feedstock to i) nitrate, ii) urea, or i) and ii), where enhanced growth of the Type II methanotrophs is established, and exposing the Type II methanotrophs to an unbalanced growth condition where production of polyhydroxybutyrate is induced.

The invention discloses an alcaligenes faecalis and application thereof. The alcaligenes faecalis is named alcaligenes faecalis (Alcaligenesfaecalis) NR and is stored in the China General Microbiological Culture Collection Center (CGMCC) with CGMCCNo.7543 on April 28, 2013. The alcaligenes faecalis is applicable to the treatment of nitrogen-containing organic wastewater and can be used for synchronously removing nitrogen and organic carbon in the wastewater. The alcaligenesfaecalis NR has both functions of denitriding and removing the organic carbon synchronously, only needs one aerobic stage to realize the synchronous removal of the organic carbon and nitrogen, and has the removal rate of about 80 to 90% for the organic ratio, and the removal rate of about 78 to 88% for TN (Total Nitrogen).

The invention discloses pseudomonas stutzeri and its culture, immobilization and use. The disclosed pseudomonas stutzeri is pseudomonas stutzeri WZUF25, is preserved in the China general microbiological culture collection center and has the preservation number of CGMCC NO. 7685. The pseudomonas stutzeri has wide aerobic denitrification suitable pH and temperature ranges, has a high NO3<->-N and NO2<->-N removal rate and a high NH4<->-N assimilation capability, and can provide a culture source for synchronous nitrification and denitrification. The invention also provides immobilized pseudomonas stutzeri prepared by an active carbon-calcium alginate embedding method. The immobilized pseudomonas stutzeri has a good NO3<->-N removal capability and good stability.

The invention discloses bacillus subtilis and an application thereof. The accession number of the bacillus subtilis BLG010 in the China General Microbiological Culture Collection Center is CGMCC No.5953. The bacillus subtilis BLG010 with number being CGMCC No.5953 can effectively inhibit the growth of fusarium oxysporum f. sp. cubense at low concentration and has great significance on control of banana wilt.

The present invention provides an apparatus and method for attracting mosquitoes by using the microbe product as the mosquito bait as well as simulating the human emanated odors. A bait-releasing unit/means is included to enhance the release of the mosquito bait. A well-controlled heating assembly can be used to generate a suitable thermal gradient for simulating body temperature and evaporating the bait. The microbe product comprises at least a sterilized supernatant of a bacterial incubation product. The apparatus of this invention can further include electrocution grids or insecticide webs to destroy attracted mosquitoes.

The invention belongs to the field of tools for in-situ colonization and culture systems for deep-sea microorganisms and in particular relates to a culture cabin for deep-sea microorganisms. According to the technical scheme, the uncovered culture cabin for deep-sea microorganisms comprises linear bearings (1), tension springs (2), a pressure compensation cavity (3), a fixed top plate (4), a deep-sea motor component (5), a fixed bottom plate (6), a hose (7) and a culture cabin body (8). The uncovered culture cabin has the beneficial effects that an end cover of the culture cabin body (8) is pushed away through rotation of the deep-sea motor component (5), microorganism enrichment culture is carried out in a completely open state and the end cover of the culture cabin body (8) is closed in laying and recovery processes to achieve sealing of the microorganism culture cabin body; the uncovered culture cabin can achieve microorganism enrichment culture in a deep-sea in-situ state; the safety during sample recovery can be ensured and the weight of the complete set of system can be effectively reduced by adopting the pressure compensation cavity (3).

The present invention relates to a nutritional composition, in particular directed to toddlers and/or a weaning child, said nutritional composition comprising a protein source, a source of available carbohydrates, a lipid source, at least one probiotic microorganism, and prebiotics, wherein said lipid source comprises DHA (docosahexaenoic acid). The nutritional composition improves cognitive performance, in particular learning and memory of the child. Preferably, the composition comprises iron, zinc, vitamin D and/or sialic acid. Preferably, the composition comprises a source of phospholipids rich in DHA.

The present invention discloses a composite microbial deodorant and a preparation method thereof. The composite microbial deodorant contains Rhodop seudanonas palustris, Candida utilis, Lactobacillus acidophilus, Streptomyces griseus and Bacillus subtilis, wherein the Rhodop seudanonas palustris, the Candida utilis, and the Lactobacillus acidophilus are subjected to mixed fermentation in a light-transmitting temperature-controllable fermentation tank, the Streptomyces griseus and the Bacillus subtilis are subjected to mixed fermentation in an aerating sterile air temperature-controlled fermentation tank, and the two fermented bacterial agents are mixed and added with essence, citric acid and glacial acetic acid to prepare the composite microbial deodorant. The composite microbial deodorant of the present invention has characteristics of simple preparation process and no secondary pollution, and provides good odor removing effects for toilets, farms and other occasions.

The present invention provides methods for screening antimicrobial agents that make use of mutated microbial polypeptides. Because these polypeptides have biological activity and result in increased antimicrobial drug resistance, candidate compounds that specifically target these polypeptides provide therapeutics or therapeutic lead compounds for treating infections caused by drug resistant microbial pathogens.

The invention discloses a bacterial strain for producing laccase, a method for producing laccase by the bacterial strain, the produced laccase and application of the laccase, and relates to the field of microbial enzyme. The bacterial strain for producing laccase is trametes pubescens (Trametes pubescens BJFC-C1108) with a microbial preservation number of CGMCC No. 10308. By utilizing the trametes pubescens (Trametes pubescens BJFC-C1108), the yield of the produced laccase is relatively high; the enzyme activity can be up to 28.687 U/mL; the prepared laccase has relatively high activity, stability and tolerance under the alkali condition (pH 7.0-10.0), has relatively high tolerance for metal ions, can be used for shortening the time required by complete decoloring of azo dye Congo red to a certain extent, has a potential application value, can be applied to more industrial fields and has favorable development and application prospects.

The invention discloses a microbial dust suppressant and its application. The microbial dust suppressant consists of 2.0wt%-3.0wt% of microbial powder, 29.0wt%-30.0wt% of calcium source and 67.0wt%-69.0wt% of ionized water Composition, described microorganism is bacillus lysinus, and described calcium source is calcium chloride. The microbial dust suppressant is evenly sprayed on the sandy soil surface under the condition of the ambient temperature of 2°C≤T≤20°C, uses microorganisms to capture carbon dioxide in the environment and converts it into carbonate, and reacts with an external calcium source to form a gelling effect The carbonate minerals have successfully consolidated loose sand particles into a whole and have certain mechanical properties. Compared with traditional methods, this method has significant dust suppression effect, low cost, convenient construction, no secondary pollution, and good ecological compatibility.

The invention discloses a method for producing microbial grease by fermenting inulin serving as a raw material. The method comprises the following steps of: hydrolyzing the inulin raw material by using inulase to prepare an inulin hydrolysate, adding yeast extract, peptone, potassium dihydrogen phosphate and MgSO4.7H2O into the inulin hydrolysate to obtain an inulin hydrolysate fermentation medium, inoculating trichosporon cutaneum seed liquid to the fermentation medium, performing fermentation culture, obtaining microbial grease bacteria by centrifuging after culture, drying the microbial grease bacteria, extracting the grease in the cells of the dried microbial grease bacteria by using an organic solvent chloroform-methanol method, and thus obtaining the microbial grease. By the method for producing the microbial grease by using the inulin, the grease raw material source of biodiesel is greatly expanded, the cost of the grease raw material for the biodiesel is remarkably lowered, and meanwhile, the problem of low value utilization of jerusalem artichoke tubers in the prior art is solved.

The invention belongs to the technical field of microbes, and particularly relates to a high-yield production method of lovastatin and monascus used by the production method. The production method comprises the following steps: strain mutagenesis and fermentation by using the mutagenesis strain. Compared with the conventional monascus production strain and production method, the mutagenesis strain and method for producing lovastatin by using the mutagenesis strain have the following advantages: the lovastatin yield can reach 1650 mg/L above, the lovastatin productivity is higher, and the lovastatin quality is improved.

The invention provides soil ecological system restoring organic fertilizer which is high in content of nutrients available for crops and reasonable in nutrition collocation, is thoroughly decomposed, has a small number of foreign microbes and is high in organic matter content. The soil ecological system restoring organic fertilizer comprises organic raw materials, microbial agents and NPK, wherein the organic raw materials comprise cake fertilizer, green manure, plant protein and biochemical humic acid; the microbial agents comprise nine microbial bacteria such as nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-releasing bacteria, actinomycetes, saccharomycetes, lactic acid bacteria, photosynthetic bacteria, paenibacillus polymyxa and bacillus subtilis; the NPK accounts for 5-25% of the total fertilizer; the NPK comprises ammonium sulfate, ammonium chloride, ammonium nitrate phosphate, ammonium phosphate, potassium sulfate, potassium chloride, potassium nitrate, magnesium sulfate, zinc sulfate and borax; the organic raw materials are taken as the balance of the fertilizer.

The invention provides a multifunctional organic/inorganic compound fertilizer and a preparation method thereof. The compound fertilizer is composed of the following raw materials by weight percent: 25-30% of urea, 5-10% of monoammonium phosphate, 10-18% of potassium sulphate, 27-32% of humic acid, 1.5-3% of zinc sulfate, 0.1-0.3% of microorganism bacterial liquid, 12-20% of diatomite and 1.9-2.7% of sophorolipid, wherein humic acid is obtained from decomposed coal through activation treatment. During preparation, urea, monoammonium phosphate, potassium sulphate, humic acid, zinc sulfate, microorganism bacterial liquid, diatomite and sophorolipid are added to a mixing crusher for crushing, so that a powdery mixture is obtained; the powdery mixture is added to a drum coating-pelleting-drying-cooling integrated machine to be pelleted, dried and cooled so that a granular mixture is obtained; the microorganism bacterial liquid and the diatomite are mixed and then sprayed to the mixture so that a crude product of the multifunctional organic/inorganic compound fertilizer is obtained; and finally, the crude product is added to a vibrating sieve for screening and then the obtained oversize is the multifunctional organic/inorganic compound fertilizer.

The invention discloses a process for producing arginine by microbial fermentation. Brevibacterium flavum MQA121 is adopted. The method comprises the following steps: preparing fermentation liquid, namely performing slant culture, performing shake-flask culture, performing primary seed culture, performing secondary seed culture, and continuing to ferment; extracting and refining a fermented product, namely preprocessing the fermentation liquid, performing secondary crystallization, performing color and impurity removal, performing membrane concentration and separation, drying, and packaging to obtain arginine as a finished product. The method has the benefits that organic and inorganic matters and bacteria in subsequent extracting and refining liquid of arginine can be remarkably reduced and the arginine yield can be increased; in addition, the product quality is improved and the production cost is reduced.

The invention discloses a method for lowering the content of nitrogen metabolism hazard materials in soy sauce through compound bacteria, and belongs to the technical field of microorganisms. By meansof combined fermentation of staphylococcus xylosus and Tetragenococcus halophilus, by lowering accumulation of biogenic amine and ethyl carbamate, the nitrogen metabolism hazard materials in soy sauce are controlled. In the soy sauce fermentation process, when soy sauce is mixed with finished koji and saline water according to the ratio of 1:1, when staphylococcus xylosus and Tetragenococcus halophilus are inoculated at the same time for fermentation, the total biogenic amine content in the finished soy sauce can be lowered by 64.2%, the content of ethyl carbamate is lowered by 77.2%, and meanwhile the amino nitrogen is increased by 61.1%.

The invention provides an atmospheric microorganism laser detection alarm system comprising a laser output device, a collection device, a detection device, a gathering device and a processing device, wherein the laser output device is used for generating 355nm pulse laser beams to induce NAD(P)H and riboflavin in microorganism to separately generate fluorescence signals; the collection device is used for receiving and transmitting the fluorescence signals; the detection device is used for converting the fluorescence signals into electrical signals; the gathering device is used for gathering the electrical signals; and the processing device is used for processing and analyzing the electrical signals. According to the atmospheric microorganism laser detection alarm system based on 355nm laser provided by the invention, air microorganism aerosol within a range of 150m in the morning and at night in an unknown area can be detected and alarmed by reasonably selecting component technical parameters and types; the system is light in weight and convenient to move and arrange, has the advantages of being real-time, stable, reliable and high in sensitivity, and can be applied to the fields of public security, epidemic prevention and environment monitoring, with wide market prospects.