13 results about "Microbiological culture" patented technology

The invention discloses an alcaligenes faecalis and application thereof. The alcaligenes faecalis is named alcaligenes faecalis (Alcaligenesfaecalis) NR and is stored in the China General Microbiological Culture Collection Center (CGMCC) with CGMCCNo.7543 on April 28, 2013. The alcaligenes faecalis is applicable to the treatment of nitrogen-containing organic wastewater and can be used for synchronously removing nitrogen and organic carbon in the wastewater. The alcaligenesfaecalis NR has both functions of denitriding and removing the organic carbon synchronously, only needs one aerobic stage to realize the synchronous removal of the organic carbon and nitrogen, and has the removal rate of about 80 to 90% for the organic ratio, and the removal rate of about 78 to 88% for TN (Total Nitrogen).

The invention discloses pseudomonas stutzeri and its culture, immobilization and use. The disclosed pseudomonas stutzeri is pseudomonas stutzeri WZUF25, is preserved in the China general microbiological culture collection center and has the preservation number of CGMCC NO. 7685. The pseudomonas stutzeri has wide aerobic denitrification suitable pH and temperature ranges, has a high NO3<->-N and NO2<->-N removal rate and a high NH4<->-N assimilation capability, and can provide a culture source for synchronous nitrification and denitrification. The invention also provides immobilized pseudomonas stutzeri prepared by an active carbon-calcium alginate embedding method. The immobilized pseudomonas stutzeri has a good NO3<->-N removal capability and good stability.

The invention discloses bacillus subtilis and an application thereof. The accession number of the bacillus subtilis BLG010 in the China General Microbiological Culture Collection Center is CGMCC No.5953. The bacillus subtilis BLG010 with number being CGMCC No.5953 can effectively inhibit the growth of fusarium oxysporum f. sp. cubense at low concentration and has great significance on control of banana wilt.

The invention provides an anti-aflatoxin general type monoclonal antibody hybridoma cell line and application thereof, belonging to the field of food safety immunodetection. The cell line 1G6 provided by the invention is collected in the China General Microbiological Culture Collection Center (CGMCC) with a collection number of CGMCC No.7210. After an aflatoxin complete antigen is uniformly mixed with equivalent Quick Antibody immunologic adjuvant TM, an obtained mixture is injected to immunize BALB/c mice through leg muscle. B1-KLH (fumonisin B1-Keyhole Limpet Hemocyanin) is adopted for the first time of immunization, M1-KLH (fumonisin M1-Keyhole Limpet Hemocyanin) is adopted for the second time of immunization, and an M1 complete antigen (without containing adjuvant) is adopted for the last time of impaction immunization. Splenocytes of immunized mice are fused with myeloma cells through a PEG (polyethylene glycol) method, and the hybridoma cell line 1G6 is obtained through indirect ELISA (Enzyme-Linked Immuno Sorbent Assay) and indirect competence ELISA screening and three times of subcloning. A monoclonal antibody secreted from the cell line has better affinity and detection sensitivity for aflatoxins B1, B2, G1, G2 and M1, and can be used for immunodetection of the total quantity of the aflatoxins in the food safety.

The invention discloses an HCV (hepatitis C virus) core antigen, which is a polypeptide encoded by a DNA (deoxyribonucleic acid) of which the amino acid sequence is shown in SEQ ID NO.1 and the nucleotide sequence is shown in SEQ ID NO.2. The invention also discloses a hybridoma cell line secreting monoclonal antibodies, the hybridoma cell line is named as SC16-H6 and has been preserved in the China General Microbiological Culture Collection Center on July 14th, 2011, and the preservation number is CGMCC No. 5074. The invention also discloses another hybridoma cell line secreting monoclonal antibodies, the hybridoma cell line is named as SC23-G5 and has been preserved in the China General Microbiological Culture Collection Center on July 14th, 2011, and the preservation number is CGMCC No. 5075. The invention also discloses a monoclonal antibody of an anti-HCV core antigen, which is secreted by the hybridoma cell line (preservation number: CGMCC No. 5074) or the hybridoma cell line (preservation number: CGMCC No. 5075), and has a specificity to the polypeptide shown in SEQ ID No.1.

The invention discloses bacillus velezensis D-1 as well as a preparation and application thereof. The name of the bacillus velezensis D-1 is bacillus velezensis D-1, the bacillus velezensis D-1 is preserved in the China General Microbiological Culture Collection Center on October 12, 2021, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.23579. The bacillus velezensis D-1 is named as bacillus velezensis D-1. The bacillus velezensis D-1 disclosed by the invention has a remarkable antagonistic effect on three kinds of fusarium causing taro dry rot, also has antagonistic activity on other important plant pathogenic fungi and pathogenic bacteria, and provides a new resource for biological prevention and control of various plant diseases in current agricultural production.

The invention discloses an achromobacter sp. for degrading lignin and application of the achromobacter sp. in lignin-containing wastewater treatment, and relates to the technical field of industrial wastewater treatment. The achromobacter sp. CCZU-WJ6 for degrading lignin is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the achromobacter sp. is CGMCC NO.19017. The strain can be applied to degradation of industrial lignin-containing wastewater, and particularly, when the lignin-containing wastewater is degraded, the applicable degradation culture conditions are as follows: the temperature is 20-40 DEG C, the pH value is 4-9, and the concentration of treated lignin is greatly reduced. Compared with the traditional method, thestrain and the application method of the strain have the advantages of high treatment efficiency, wide application range, simple operation, low equipment requirement and obviously reduced operation cost, and solve the problems of complex procedure and carbon source requirement in fungus treatment of industrial lignin-containing wastewater.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Botrytis cinerea. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

The invention discloses a Streptomyces bacterial strain TY-001#. The Streptomyces bacterial strain TY-001# is obtained through screening separation from soil, and is preserved at China General Microbiological Culture Collection Center, Guangdong on 24th, March, 2017, and the preservation number is GDMCC No:60152. The Streptomyces bacterial strain TY-001# is capable of adapting ventilation fermentation culture at high osmotic pressure; microorganism organic fertilizer obtained through fermentation culture of the Streptomyces bacterial strain TY-001# in soya-bean milk cane sugar culture medium is capable of promoting plant growth obviously; spraying of the microorganism organic fertilizer is capable of promoting brassica Chinensis growth, synthesized chlorophyll is increased, leaves are fatand large, stems are thick, nutrient accumulation is increased, high temperature resistance and disease and insect resistance are improved, the growth vigor is excellent, and the application value ofthe Streptomyces bacterial strain TY-001# in preparation of microorganism organic fertilizer is high.

The invention relates to the technical field of selenium yeast production, in particular to selenium yeast for feed and a preparation method of the selenium yeast. The invention relates to a preparation method of selenium yeast for feed, which comprises the following steps: step A, inoculating a strain of pichia pastoris preserved in a glycerol tube into an eggplant bottle seed culture medium, standing at 30-35 DEG C for eggplant bottle slant seed culture for 24-72 hours to obtain eggplant bottle slant seeds, the classification name of the pichia pastoris is pichia pastoris, and the classification name of the pichia pastoris is CGMCC (China General Microbiological Culture Collection Center); the pichia pastoris X33 PLT-07 strain is preserved in Guangdong Microbiological Culture Collection Center, and the preservation number is GDMCC No: 60262. The preparation method of the selenium yeast for the feed is simple, the prepared selenium yeast product is high in yield and low in production cost, the problems that an existing selenium yeast product is low in production yield and high in production cost are solved, and the prepared selenium yeast for the feed is high in organic selenium content and high in selenium content. The problem that selenium yeast prepared in the prior art is low in organic selenium content is solved.

The invention relates to a chicken Flavivirus and an inactivated vaccine thereof and belongs to the field of veterinary epidemiology. The chicken Flavivirus is chicken Flavivirus strain FQ-C1 which is preserved in the China General Microbiological Culture Collection Center with preservation serial number: CGMCC No.4905. After the chicken Flavivirus strain FQ-C1 is inoculated to SPF (specified pathogen free) chick embryos, the allantoic fluids of the SPF chick embryos which die of transfection are collected, the allantoic fluids of the SPF chick embryos are inactivated with formaldehyde and then mixed with white oil which is used as an adjuvant, and the resulting mixture is emulsified to prepare an inactivated vaccine in the form of oil emulsion. The chicken Flavivirus inactivated vaccine provided by the invention does not cause any adverse effect on chickens and has high experimental safety. The vaccine can effectively control Flavivirus disease in chickens and helps reduce the losses and increase the breeding benefits of majority of chicken farmers in China.