Selenium yeast for feed and preparation method thereof

[0024] A preparation method for feed selenium yeast, comprising the following steps:

[0025] Step A, inserting the strain of Pichia pastoris preserved in a glycerol tube into the eggplant bottle seed medium, standing still at 30-35°C to cultivate the slant seeds of the eggplant bottle, and the cultivation time is 24-72h to obtain the slant seeds of the eggplant bottle, The classification of the Pichia pastoris is named Pichia pastoris (Pichia pastoris), which has been preserved in the Guangdong Provincial Microbiological Culture Collection Center on October 26, 2017, and the preservation number is: GDMCC No: 60262 Pasteur Pichia pastoris X33 PLT-07 strain, the address of the preservation unit is: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City, Guangdong Institute of Microbiology, and the preservation unit code is: GDMCC-Guangdong Microbial Culture Collection Center, deposit The activity was tested by the Guangdong Microbial Culture Collection Cente

[0058] A preparation method for feed selenium yeast, comprising the following steps:

[0059] Step A, insert the strain of Pichia pastoris preserved in a glycerol tube into the eggplant bottle seed medium, and stand at 35°C to cultivate the eggplant bottle slant seeds for 72 hours to obtain the eggplant bottle slant seeds, the Pichia The classification of yeast is named Pichia pastoris (Pichia pastoris), which is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is: Pichia pastoris X33 PLT-07 strain of GDMCC No: 60262;

[0060] The preparation method of eggplant bottle seed medium is as follows: glucose 30g / L, peptone 30g / L, nutrient agar 150g / L and the rest of water, the pH is natural, high-temperature sterilization is carried out, and the sterilization temperature is 122°C , the sterilization and holding time is 30min;

[0061] Step B, divide the eggplant bottle slant seeds into 2cm×2cm inoculated pieces and insert them into the shak

[0068] Table 1 embodiment A1~A6 fermentation culture condition

[0069]

[0070] The total selenium content and the organic selenium content in the total selenium content and the organic selenium content in the thalline wet weight, the obtained feed selenium yeast product are measured to embodiment A1~A6, the test result obtained is as shown in table 2.

[0071] Table 2 embodiment A1~A6 test result

[0072]

[0073] Known by embodiment A1~A6, when the fermentation temperature that the step D of embodiment A4~A6 carries out fermentation culture is too high, will influence the wet weight of thalline when fermentation culture, and reduce the selenium yeast for feed that makes The organic selenium content in the thalline of embodiment A4~A6 is significantly lower than embodiment A1~A3 when the thalline of embodiment A4~A6 finishes fermentation culture, and the organic selenium content in the selenium yeast for feed that embodiment A4~A6 makes is also Obviously lower than the o