219results about "Microorganism based processes" patented technology

The invention discloses a solid microbial agent used for remedying the soil contaminated by petroleum, belonging to the technical field of bioremediation of soil contaminants. First-class seed liquid of acinetobacter radioresistens and rhodococcus erythropolis are mixed in equal parts after two steps of fermentation in liquid and solid states and cultivation to obtain the solid microbial agent, wherein, the number of effective viable bacteria of acinetobacter radioresistens and rhodococcus erythropolis is 10 to 10 CFU/g. The solid microbial agent in the invention has the advantages of low cost, high number of viable bacteria; the invention further has the advantages that micro-ecological environment improvement of the soil can be improved and secondary contamination cannot be caused to the environment, thus applicable to the bioremediation of the soil which is contaminated by petroleum and petroleum products.

The invention discloses a preparation method of nemadectin. According to the invention, a fermentation broth used for producing nemadectin is directly dried by spraying, such that solid powder is obtained; the solid powder is extracted by using an organic solvent, the extract is filtered, and nemadectin in the extract is absorbed by using macroporous resin; nemadectin absorbed by macroporous resin is then eluted; and the eluent is extracted, concentrated, and dried, such that a nemadectin pure product is obtained. According to the invention, a direct spray-drying method is adopted upon the fermentation broth, and the obtained dried solid fermentation bacterium powder is transportation-tolerant, and is easy to store. With the method provided by the invention, the producing of nemadectin degradation product is reduced, the bacterium powder is easy to extract, and the consumption of the extraction solvent is low. The technology is simple, the cost is low, and the method is easy to operate. With the method, the prepared nemadectin has a high HPLC purity of above 90%.

The invention discloses a method for producing astaxanthin by inducing Haematococcus pluvialis, which is characterized by comprising the following steps: in the vegetative growth phase, inoculating Haematococcus pluvialis in a nutrient culture medium, and culturing; continuously keeping irradiation on the Haematococcus pluvialis and ensuring that the intensity of illumination arriving at the Haematococcus pluvialis cell surface is 20-200 mu mol.m<-2>.s<-1>; in the induction stage, adding ethanol and seawater crystal into the production culture medium, wherein the ethanol added into the production culture medium accounts for 0.5-3 vol%, and the seawater crystal added into the production culture medium accounts for 0.5-1 wt%; and continuously introducing filtered air mixed with 1-3 vol% carbon dioxide into the production culture medium, wherein 0.2-0.4L/minute of the air is introduced to every 1L of production culture medium. The method can induce the Haematococcus pluvialis to quickly accumulate the astaxanthin, is simple and has the advantages of high yield and favorable effect.

The invention discloses an alcaligenes faecalis and application thereof. The alcaligenes faecalis is named alcaligenes faecalis (Alcaligenesfaecalis) NR and is stored in the China General Microbiological Culture Collection Center (CGMCC) with CGMCCNo.7543 on April 28, 2013. The alcaligenes faecalis is applicable to the treatment of nitrogen-containing organic wastewater and can be used for synchronously removing nitrogen and organic carbon in the wastewater. The alcaligenesfaecalis NR has both functions of denitriding and removing the organic carbon synchronously, only needs one aerobic stage to realize the synchronous removal of the organic carbon and nitrogen, and has the removal rate of about 80 to 90% for the organic ratio, and the removal rate of about 78 to 88% for TN (Total Nitrogen).

The present invention relates to a biological control strain AR156 for preventing and treating root-knot nematodes of greenhouse vegetable, belonging to plant protection technical field. The bacteria is bacillus sp., which is cultivated in PDA culturing liquor under 37 degree and 180rpm, then is centrifuged 10min under 6000rpm. Picking wet bacteria and bacteria storage liquid is prepared into bacteria agent according to 1:40 (g/ml)proportion. The living bacteria concentration is 1*109-1*1012cfu/ml.The effective rate of the bacteria agent amount to 58-75% for preventing root-knot nematodes of cucumber, towel gourd, tomato etc, and yield increase more than 30-50%. The bacteria agent can also treat wilt, epidemic disease and soil hardening. The bacteria agent can be sprayed, composted and irrigated to root.

The invention discloses pseudomonas stutzeri and its culture, immobilization and use. The disclosed pseudomonas stutzeri is pseudomonas stutzeri WZUF25, is preserved in the China general microbiological culture collection center and has the preservation number of CGMCC NO. 7685. The pseudomonas stutzeri has wide aerobic denitrification suitable pH and temperature ranges, has a high NO3<->-N and NO2<->-N removal rate and a high NH4<->-N assimilation capability, and can provide a culture source for synchronous nitrification and denitrification. The invention also provides immobilized pseudomonas stutzeri prepared by an active carbon-calcium alginate embedding method. The immobilized pseudomonas stutzeri has a good NO3<->-N removal capability and good stability.

The invention discloses bacillus subtilis and an application thereof. The accession number of the bacillus subtilis BLG010 in the China General Microbiological Culture Collection Center is CGMCC No.5953. The bacillus subtilis BLG010 with number being CGMCC No.5953 can effectively inhibit the growth of fusarium oxysporum f. sp. cubense at low concentration and has great significance on control of banana wilt.

The invention relates to a processing method of changing food waste into resources. Ethanol, methane, liquid fertilizers, solid fertilizers, pet food and the like can be obtained through the following steps of food waste solid and liquid separation and respectively treatment after the food, the potato type, the vegetable melon and fruit type, the animal meal and fish bone type and rubbish sundries are sorted from the solid, and oil for producing biodiesel, soap and glycerin, purified water used for fish culture, water plant planting and farmland and garden irrigation and the like can be obtained after liquid treatment. The methane is used for combustion after being dewatered, and is used for electric power generation after being desulfurized, and the waste heat of the electric power generation is reused. The method of the invention fully utilizes waste materials, produces various products, and has the advantages of low energy consumption and high yield.

The invention provides a clone and a pronucleus expression method of a novel B class epoxide hydrolase gene mature peptide cDNA sequence from Aspergillus usamii E001. The nucleotide sequence of the novel B class epoxide hydrolase gene mature peptide cDNA sequence is SEQ ID NO:1, the corresponding amino acid sequence is SEQ ID NO:2, and the corresponding gene is named Aus EH-B. According to the invention, good stereoselectivity is achieved for (R)-epichlorohydrin through chiral gas chromatography analysis rEH, and a produced (S)-epichlorohydrin antipode excess value achieves 99%. The pronucleus expression method provided by the invention lays the foundation for the industrialized production of epoxide hydrolase and provides the basis for preparing chiral epichlorohydrin by researching an EH enzyme kinetic resolution method for biological catalysis technology industrialization.

The invention discloses denitrifying phosphorus removal bacteria bacillus cereus H-hrb01, and belongs to the technical fields of biological engineering and environmental engineering, wherein bacillus cereus H-hrb01 belongs to bacillus genus by identification and is conserved in the general microbiology centre of the China committee for culture collection of microorganisms, the conservation date is March 27th 2012, and the conservation number is CGMCC NO.5939; bacillus cereus H-hrb01 can take various organic matters as carbon sources, has good denitrifying and phosphorus-removing efficiency, and can be used for sewage biological denitrification and dephosphorization process; bacillus cereus H-hrb01 is activated and cultivated, and fed into artificial wastewater to mix and then introduced into an SBR (styrene butadiene rubber) biofortification device, and each effluent index of the reactor is superior to that of a contrast system which is not fed with the bacterial strain. The removal rates of the COD (chemical oxygen demand), ammonia nitrogen and dissoluble phosphorus in the sewage are respectively up to 93.90%, 97.70% and 96.25%.

The present invention discloses a high-yield strain of high temperature resistant 1,4-beta-D-xylanase, and a method for producing the high temperature resistant 1,4-beta-D-xylanase through fermentation of the high-yield strain, and provides partial enzymatic properties of the high temperature resistant 1,4-beta-D-xylanase. After the strain identification, the high-yield strain is named Paenibacillus campinasensis G1-1, and is preserved in the China general microbiological culture collection center. The preservation number of the strain is CGMCC No. 5023. According to the present invention, the high temperature resistant 1,4-beta-D-xylanase produced through the fermentation of the high-yield strain is subjected to separation and purification to obtain a single component, wherein the single component has a relative molecular weight of 41.3 KDa, an optimal operation temperature of 60 DEG C and an optimal operation pH of 7.0; the relatively stable enzyme activity of the high temperature resistant 1,4-beta-D-xylanase is remained in the temperature range of 40-70 DEG C and the pH range of 5.0-9.0; the Paenibacillus campinasensis G1-1 provided by the present invention is applicable for a plurality of fields such as paper making, food, feedstuff and the like, and has a broad application prospect.

The invention belongs to the technical field of animal infectious disease prevention and curing, and particularly relates to application of mycoplasma bovis MbovP730 protein in natural infection and vaccine immunity identification. According to the codon preference of escherichia coli, the Mbov_730 gene of M.bovis HB0801 is cloned and is modified, and a mycoplasma bovis tryptophan codon UGA is mutated into a tryptophan codon UGG in the escherichia coli so as to express recombinant mycoplasma bovis MbovP730 protein in the escherichia coli. The nucleotide sequence of the MbovP730 protein is disclosed in SEQ ID NO: 13, and the protein can be used as the antigen protein to be applied to the preparation of a kit for identifying mycoplasma bovis wild strain infection and vaccine strain immune identification.

The invention discloses Escherichia coli and a method for preparing L-cysteine by using the same. Escherichia coli BL21(DE3) is used as a template for the genome of the Escherichia coli, and the template has serine acetyltransferase shown as a sequence in SEQ ID NO.1 and in deletion of a gene metC, a gene tnaA, a gene gshA and a gene dfp. The method comprises the following steps of: firstly, putting the Escherichia coli in a seed culture medium for culturing; and then putting the Escherichia coli in a fermentation culture medium to ferment to obtain the L-cysteine. The invention has the advantages that by utilizing the Escherichia coli to prepare the L-cysteine, the conversion rate of a substrate and the output of the L-cysteine are greatly improved; the conversion rate of the substrate can reach 30 percent improved by more than 50 percent compared with that of the prior art; and the output of the L-cysteine can be improved by more than 20 percent compared with that of the prior art.

The invention provides a microbe water purifying additive and a preparation method thereof and an application thereof. The preparation of the microbe water purifying additive is as follows: aspergillus niger CCAM080003 is subject to activation, expansion and fermentation culture, and the culture materials collected are dried and crushed, and the additive is prepared. The microbe water purifying additive of the invention contains hydrolase such as hot activity cellulase, xylanase, dextranase, prolease and lipase, etc., and can accelerate the decomposition of organic matters in the sewage and improve the water treatment efficiency, so the microbe water purifying additive is applied to the water purification treatment in ponds, sewage treatment plants and scenic water, etc. The microbe water purifying additive of the invention can be joined with the microbe purifying agent to purify water; compared with the independent use of the microbe purifying agent, the addition of the water purifying additive can greatly improve the speed of purifying the water and obviously improving the purifying effect.

The invention discloses a bacterial strain for producing laccase, a method for producing laccase by the bacterial strain, the produced laccase and application of the laccase, and relates to the field of microbial enzyme. The bacterial strain for producing laccase is trametes pubescens (Trametes pubescens BJFC-C1108) with a microbial preservation number of CGMCC No. 10308. By utilizing the trametes pubescens (Trametes pubescens BJFC-C1108), the yield of the produced laccase is relatively high; the enzyme activity can be up to 28.687 U/mL; the prepared laccase has relatively high activity, stability and tolerance under the alkali condition (pH 7.0-10.0), has relatively high tolerance for metal ions, can be used for shortening the time required by complete decoloring of azo dye Congo red to a certain extent, has a potential application value, can be applied to more industrial fields and has favorable development and application prospects.

The invention provides an anti-aflatoxin general type monoclonal antibody hybridoma cell line and application thereof, belonging to the field of food safety immunodetection. The cell line 1G6 provided by the invention is collected in the China General Microbiological Culture Collection Center (CGMCC) with a collection number of CGMCC No.7210. After an aflatoxin complete antigen is uniformly mixed with equivalent Quick Antibody immunologic adjuvant TM, an obtained mixture is injected to immunize BALB/c mice through leg muscle. B1-KLH (fumonisin B1-Keyhole Limpet Hemocyanin) is adopted for the first time of immunization, M1-KLH (fumonisin M1-Keyhole Limpet Hemocyanin) is adopted for the second time of immunization, and an M1 complete antigen (without containing adjuvant) is adopted for the last time of impaction immunization. Splenocytes of immunized mice are fused with myeloma cells through a PEG (polyethylene glycol) method, and the hybridoma cell line 1G6 is obtained through indirect ELISA (Enzyme-Linked Immuno Sorbent Assay) and indirect competence ELISA screening and three times of subcloning. A monoclonal antibody secreted from the cell line has better affinity and detection sensitivity for aflatoxins B1, B2, G1, G2 and M1, and can be used for immunodetection of the total quantity of the aflatoxins in the food safety.

The present invention relates to a method of isolating cells from a sample which method comprises binding said cells to a solid support by means of a non-specific ligand immobilised on said solid support, particularly to a method of isolating microorganisms from a sample. Preferred ligands for use in such methods include carbohydrates and derivatives thereof. Also described is a kit for isolating microorganisms from a sample comprising: (a) a solid support having immobilised thereon a ligand which is capable of non-specific binding to microorganisms; (b) means for binding microorganisms to said solid support; optionally (c) means for lysing said cells; and optionally (d) means for binding nucleic acid released from said lysed cells to a solid support.

The invention discloses a method for producing microbial grease by fermenting inulin serving as a raw material. The method comprises the following steps of: hydrolyzing the inulin raw material by using inulase to prepare an inulin hydrolysate, adding yeast extract, peptone, potassium dihydrogen phosphate and MgSO4.7H2O into the inulin hydrolysate to obtain an inulin hydrolysate fermentation medium, inoculating trichosporon cutaneum seed liquid to the fermentation medium, performing fermentation culture, obtaining microbial grease bacteria by centrifuging after culture, drying the microbial grease bacteria, extracting the grease in the cells of the dried microbial grease bacteria by using an organic solvent chloroform-methanol method, and thus obtaining the microbial grease. By the method for producing the microbial grease by using the inulin, the grease raw material source of biodiesel is greatly expanded, the cost of the grease raw material for the biodiesel is remarkably lowered, and meanwhile, the problem of low value utilization of jerusalem artichoke tubers in the prior art is solved.

The invention belongs to the technical field of microbes, and particularly relates to a high-yield production method of lovastatin and monascus used by the production method. The production method comprises the following steps: strain mutagenesis and fermentation by using the mutagenesis strain. Compared with the conventional monascus production strain and production method, the mutagenesis strain and method for producing lovastatin by using the mutagenesis strain have the following advantages: the lovastatin yield can reach 1650 mg/L above, the lovastatin productivity is higher, and the lovastatin quality is improved.

The invention discloses a 1,3-1,4-beta-glucanase mutant, and belongs to the fields of gene engineering and enzyme engineering. Lysine in the 20th position, 117th position and 165th position of 1,3-1,4-beta-glucanase from Bacillus terquilensis mutates through an overlapping extension PCR method to form serine in order to obtain single mutants K20S, K117S and K165S respectively. The above three mutation sites are integrally mutated to obtain a K20S/K117S/K165S triple mutant enzyme. Four mutant enzymes have higher catalysis activity and better thermal stability. Compared with wild enzymes, the above mutant enzymes are in favor of realizing the industrial application.

The invention discloses a culturing method for the mycelia of Antrodia camphorata, belonging to the field of biological fermentation technology. The method of the invention comprises the following steps: inoculating a slant culture medium with a Antrodia camphorata strain for activation and purification so as to obtain an activated mother strain; inoculating a liquid shake-flask culture medium with the activated mother strain for static culture, and carrying out stirring culture after mycelia germinate and are free of pollution so as to obtain a shake-flask strain; inoculating a fermentation medium with the shake-flask strain for fermentation culture so as to obtain fermented mycelia; and inoculating a artificially synthesized solid culture medium with the fermented mycelia which are usedas a liquid strain and carrying out fermentation for 30 to 90 days so as to obtain the mycelia of Antrodia camphorata. The method of the invention has the advantages of a short cultivation period forthe mycelia of Antrodia camphorata, a high culture success rate of and high medicinal value of the mycelia.

The invention discloses a CRISPR-Cas12a detection primer group for francisella tularensis and an application thereof. The invention provides the primer group for detecting francisella tularensis and is composed of an RPA primer pair and crRNA; the RPA primer pair is designed according to the nucleotide sequence shown in SEQ ID NO.1 in the francisella tularensis genome; the crRNA comprises an anchoring sequence and a guide sequence, wherein the anchoring sequence can be combined with a cas protein, and the guide sequence is matched with an amplification product sequence of an RPA primer pair. The detection primer group has the characteristics of short detection time, high amplification efficiency, high sensitivity, strong specificity and the like, and the detection sensitivity on francisella tularensis positive plasmids can reach 900 copy/ml, and the detection sensitivity on the francisella tularensis genome can reach 1260 fg/mL. The francisella tularensis onsite diagnosis method has awide application prospect.

The invention discloses a strain for generating pullulan. The strain is a strain which is obtained from plant leaves and does not generate melanin. The classification and nomenclature of the strain is Aureobasidium pullulans HL-1 as a Latin name. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.10772. A method for producing the pullulan by fermenting the strain is simple and feasible; a product is high in conversion rate so as to facilitate later-stage separation and purification.