The invention relates to an industrial production method of a dry rat
glycolipid bio-surfactant
powder. The method comprises the following steps of: (1) breeding a strain; (2) slantly culturing the strain; (3) carrying out enlarged culture on the strain; (4) industrially fermenting and culturing the strain; and (5) centrifuging a produced
lipopeptide bio-surfactant
fermentation liquor by a high speed centrifugal
machine at a rotation speed of 8000 rpm / min, so as to respectively obtain a
lipopeptide separating liquor and
bacillus subtilis. The
surface tension of the stoste of the
lipopeptide bio-surfactant
fermentation liquor produced with the method provided by the invention is less than 26 mN / m; and after being centrifuged to remove thalli and then diluted by 500 times, the stoste of the lipopeptide bio-surfactant
fermentation liquor obtains the
surface tension of less than 34 mN / m. The separated lipopeptide separating liquor can be used for
ternary complex flooding oil-extraction; and the flooding effect of the lipopeptide separating liquor can be improved by 8-16% on the basis of
water flooding. It is detected that the bacterial count of the separated
bacillus subtilis can reach more than 10 billions in every 6 ml of the fermentation liquor; and the
bacillus subtilis can be used for producing and
processing EM (E Mycin) bacterial liquids and is applied to the fields, such as
agriculture,
livestock breeding, and
sewage treatment (industrial
sewage and breeding
sewage).