18 results about "Bacillus subtilis" patented technology

The invention discloses bacillus subtilis and an application thereof. The accession number of the bacillus subtilis BLG010 in the China General Microbiological Culture Collection Center is CGMCC No.5953. The bacillus subtilis BLG010 with number being CGMCC No.5953 can effectively inhibit the growth of fusarium oxysporum f. sp. cubense at low concentration and has great significance on control of banana wilt.

The present invention discloses a composite microbial deodorant and a preparation method thereof. The composite microbial deodorant contains Rhodop seudanonas palustris, Candida utilis, Lactobacillus acidophilus, Streptomyces griseus and Bacillus subtilis, wherein the Rhodop seudanonas palustris, the Candida utilis, and the Lactobacillus acidophilus are subjected to mixed fermentation in a light-transmitting temperature-controllable fermentation tank, the Streptomyces griseus and the Bacillus subtilis are subjected to mixed fermentation in an aerating sterile air temperature-controlled fermentation tank, and the two fermented bacterial agents are mixed and added with essence, citric acid and glacial acetic acid to prepare the composite microbial deodorant. The composite microbial deodorant of the present invention has characteristics of simple preparation process and no secondary pollution, and provides good odor removing effects for toilets, farms and other occasions.

The invention provides a soil bio-activator for ginseng continuous cropping and belongs to the field of modernization of Chinese traditional medicine. The soil bio-activator for ginseng continuous cropping can be used for solving the problems of serious ginseng continuous cropping diseases, low yield and poor quality caused by deterioration of the physicochemical and biological characters of old ginseng-grown land soil. The soil bio-activator for ginseng continuous cropping is prepared from 2.0%-8.0% of bacillus subtilis, 2.0%-8.0% of actinomycetes, 5.0%-20.0% of xylanase, 5.0%-20.0% of cellulase, 10.0%-20.0% of urease, 1.0%-4.0% of potassium dihydrogen phosphate, 0.1%-0.5% of ferrous sulfate, 0.1%-0.4% of boric acid, 0.1%-0.5% of manganese sulfate, 0.05%-0.2% of copper sulfate, 0.1%-0.4% of zinc sulfate, 0.05%-0.2% of ammonium molybdate and the balance of organic materials through mixing and compositing. The soil bio-activator for ginseng continuous cropping is used for improving the soil for ginseng continuous cropping in ginseng growing.

The invention provides soil ecological system restoring organic fertilizer which is high in content of nutrients available for crops and reasonable in nutrition collocation, is thoroughly decomposed, has a small number of foreign microbes and is high in organic matter content. The soil ecological system restoring organic fertilizer comprises organic raw materials, microbial agents and NPK, wherein the organic raw materials comprise cake fertilizer, green manure, plant protein and biochemical humic acid; the microbial agents comprise nine microbial bacteria such as nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, potassium-releasing bacteria, actinomycetes, saccharomycetes, lactic acid bacteria, photosynthetic bacteria, paenibacillus polymyxa and bacillus subtilis; the NPK accounts for 5-25% of the total fertilizer; the NPK comprises ammonium sulfate, ammonium chloride, ammonium nitrate phosphate, ammonium phosphate, potassium sulfate, potassium chloride, potassium nitrate, magnesium sulfate, zinc sulfate and borax; the organic raw materials are taken as the balance of the fertilizer.

The invention discloses multipath composite neuraminic acid producing bacillus subtilis and an application thereof, and belongs to the field of genetic engineering. The expression levels of key enzymes, namely UDP-N-acetylglucosamine-2-epimerase, N-acetylglucosamine-6-phosphate isomerase, glucosamine-6-phosphoric acid-N-acetyltransferase, N-acetylglucosamine isomerase and N-acetylneuraminate synthase on genomes in three neuraminic acid synthesis paths are sequentially optimized by 10 promoters with different intensities, so that the protein synthesis pressure of enzyme expression on cells is reduced; and three neuraminic acids are further integrated into the same engineering bacillus subtilis, so that the bacillus subtilis with the increased N-acetylneuraminic acid yield is obtained, the neuraminic acid yield under the shake flask level reaches 10.4 g/L, and a foundation is laid for further increasing the NeuAc yield of the bacillus subtilis.

The invention discloses a method for remediating soil polluted by Cu, Pb and As by inoculating sunflower and castor with bacillus subtilis and relates to the technical field of environmental remediation. The method includes: preparing a bacterial suspension, inoculating seeds with the bacterial suspension, preprocessing soil, culturing, managing, removing, performing subsequent processing and thelike. The method has the advantages that Cu, Pb and As are gradually transferred into the plant bodies of the castor inoculated with the bacillus subtilis and the sunflower inoculated with the bacillus subtilis during the growth of the plant bodies, and then the whole plant bodies are removed from the soil so as to remove the excessive Cu, Pb and As in the soil; the method is good in remediating effect on the soil polluted by Cu, Pb and As and easy to implement and popularize.

The invention discloses a highland barley biological control disease-resistant and growth promoting method. The highland barley biological control disease-resistant and growth promoting method comprises the steps that bacillus subtilis are made into high density bacterial suspension with the density ranging from 109cfu/mLto 1010cfu/mL; healthy highland barley straws are cut into small sections to compost, the bacillus subtilis high density bacterial suspension is sprayed in the composting process, a biological fertilizer capable of effectively restraining and killing highland barley pathology funguses is obtained after fermentation and is used on highland barley biological control disease-resistant and growth promotion. According to the highland barley biological control disease-resistant and growth promoting method, the bacillus subtilis producing beta dextranase are plant probiotics with a disease-resistant and growth promoting function, the biological fertilizer is added into the compost to effectively strengthening the biological control function on highland barley disease funguses, restraining and killing effects on sheath rot pathogenic bacteria are showed, and the highland barley yield is improved. According to the highland barley biological control disease-resistant and growth promoting method, treatment of the composting process is easy, no waste water is discharged, the investment is small, the energy consumption is low, the operation is easy, industrialized mass production is easy to achieve, the economic benefit is good, and the application prospect is wide.

The invention discloses a microbial water purifier for aquaculture water bodies, wherein the microbial water purifiers for aquaculture water bodies are activated bentonite particles immobilized with Bacillus subtilis coagulans. The present invention also provides a preparation method of the above-mentioned microbial water purifying agent for aquaculture water. The microbial water purifier for aquaculture water of the present invention adopts the bentonite as the immobilization carrier, takes the coagulated Bacillus subtilis as the immobilization object, and increases the value of the immobilized bacteria through the co-cultivation, the amount of immobilized bacteria is large, and the enrichment efficiency is high; all the materials used are non-toxic It is harmless, has no secondary pollution, has low cost, is convenient for transportation and storage, and can realize large-scale production.

The invention discloses application of sucrose phosphorylase in preparation of 2-O-alpha-D-glucosyl-L-ascorbic acid. According to the application, a reaction system is formed by taking fermentation liquor, obtained through fermenting sucrose phosphorylase gene containing engineering bacteria, or broken liquor, obtained through resuspending wet thalli obtained after centrifuging the fermentation liquor with a buffer solution, as a catalyst, taking L-ascorbic acid as a substrate, taking sucrose as a cosubstrate and taking a buffer solution with a pH of 4.8 to 6.0 as a reaction medium, and a reaction is carried out at the temperature of 30 DEG C to 55 DEG C, thereby obtaining AA-2G. According to the application, the AA-2G is efficiently produced by employing the sucrose phosphorylase, the yield is 3.47g/L/h and is increased by 34.5% compared with that employing the existing reported B. longum derived sucrose phosphorylase, and the maximum concentration of the AA-2G can reach 178g/L; and recombinant bacillus subtilis can efficiently secrete and express the sucrose phosphorylase, and the fermentation liquor is directly applied to preparation of food-grade AA-2G.

The invention discloses a preparation technology of a biological fermentation treatment agent used for treating dead diseased pigs. The preparation technology includes the steps that by weight, thermophilic aerobiotic bacilli, issatchenkia orientalis, bacillus subtilis, nitrifying bacteria, bifidobacterium and thermophilic archaeosphaeroides are separately fermented and then mixed with keratinaseand maltodextrin uniformly by weight, so that the biological fermentation treatment agent is prepared. All the raw materials of the biological fermentation treatment agent supplement on another and realize synergistic interaction, and the presentation of the final effect of the biological fermentation treatment agent is based on the cooperative relationship of all the raw materials. Biological fermentation fertilizer prepared from the biological fermentation treatment agent is used for planting corn, in this way, the average yield per mu is increased greatly, the effect of using is good, preparation costs are low, ecological environmental protection is realized, and application and popularization are easy.

The invention relates to a method for preparing a bacterial fertilizer from glutamic acid fermentation waste liquid and application of the bacterial fertilizer, belonging to the technical field of vegetable straw mulching. The method comprises the following steps: S1, collecting vegetable straw waste and then cutting the collected vegetable straw waste into sections so as to obtain straw waste A;S2, mixing the glutamic acid fermentation waste liquid, the straw waste A and water to obtain a mixture A; S3, adding saccharomycetes, lactic acid bacteria, bacillus subtilis and trichoderma aureoviride into the mixture A, and carryin gout natural fermenting to obtain a fermented material A; and S4, adding an organic nitrogen source into the fermented material A, and carrying out sealed fermentation to obtain the zymophyte fertilizer. The invention also provides the application of the zymophyte fertilizer prepared by using the method in promoting vegetable growth. According to the invention, the industrial glutamic acid fermentation waste liquid and agricultural vegetable straw waste can be treated at the same time, and the prepared zymophyte fertilizer is rich in nutrients and good in economic benefits.

The invention discloses an efficient phenylamine degradable bacterial preparation and a preparation method thereof. The preparation comprises the following raw materials in percent by mass: 5-10% of pseudomonas putida, 10-15% of candida mycoderma bacteria, 7-13% of bacillus amyloliquefaciens, 6-11% of paper-soluble clostridum, 8-15% of trichoderma aureoviride, 2-6% of nitrobacterium, 4-7% of pseudomonas aeruginosa, 3-5% of bacillus subtilis, 11-16% of alcaligenes faecalis, 5-8% of rhodococcus and 4-9% of bacillus. A certain amounts of the prepared bacterial preparation is put into wastewater,then degradation of the bacterial preparation upon pollutants can be accelerated, and the bacterial preparation comprises multiple microorganisms with excellent refractory pollutant degradation capabilities, the strains are reasonably matched, coexist harmoniously, are not antagonized mutually, are high in activity, are large in biomass and rapid in propagation, and have a good degradation effecton phenylamine polluted water bodies when being used in wastewater.

The present invention discloses a culture medium combination for promoting natural growth and transformation of bacillus subtilis and a transformation method. The culture medium combination comprisesa culture medium 1 and a culture medium 2. The culture medium containing a special nutrient formula is utilized to efficiently obtain bacillus subtilis competent cells, the natural transformation method is cooperated and beneficial recons are more efficiently and quickly obtained, thus a complex method that can only rely on electrotransformation of the bacillus subtilis is improved, transformationcost is greatly reduced, and foundation for directed evolution and metabolic engineering of proteins of the bacillus subtilis is laid. The technology overcomes shortcomings of traditional transformation technology, and the method is simple and easy to implement, high in conversion efficiency and easy to popularize.

The invention relates to a microorganism and enzyme mixed preparation for stripping dirt of aquatic plants as well as a preparation method and application thereof. The microorganism and enzyme mixed preparation comprises citric acid, starch, carboxymethyl chitosan, bacillus subtilis, cellulase, cysteine and diatomite. The preparation method of the stripping agent comprises the following steps of: 1, sequentially adding the substances according to the proportion; 2, uniformly stirring for 15 minutes by using a V-shaped stirrer or a three-V stirrer; and 3, mixing the obtained mixture with pond water according to a mass ratio of 1: 100 to 1: 200, and uniformly stirring to obtain the microorganism and enzyme mixed preparation for stripping dirt of aquatic plants. Dirt on the surfaces of aquatic plants is rapidly removed, and photosynthesis is improved. A high-quality ecological environment rich in aquatic plants, which is clear in water, high in dissolved oxygen content in water and few in harmful substances in the water, is provided for cultured objects.