39 results about "Gene" patented technology

The invention provides a gene data analysis method and a heterogeneous scheduling platform. Assembly line management is performed on tasks to be executed through interaction among a controller, a GPU,an FPGA and a CPU by means of load balancing. The method specifically comprises the following steps: reading and processing a to-be-analyzed gene sequencing file, and storing obtained gene data intoa memory; and reading the gene sequence in the memory and allocating the gene sequence to the processor which is currently in an idle state and/or is not overloaded by combining the load and the running state of the processor by the controller, executing multi-task computational analysis, correcting and converting a computational result, generating an analysis report according to statistics standard output information, and completing data analysis. Load balance is monitored in real time in the analysis process, and if the load for processing the current task is in shortage, computing resourceacceleration processing is improved; correspondingly preposed task computing resources are reduced to reduce input; and the efficiency of analyzing and interpreting the multi-task gene data in unit time is greatly improved.

Genetically modified lactic acid bacteria having a reduced or lacking or enhanced diacetyl reductase activity, acetoin reductase activity and/or butanediol dehydrogenase activity are provided. Such bacteria are used in starter cultures in the production of food products including dairy products where it is desired to have a high content of diacetyl and for reducing or completely removing diacetyl in beverages including beers, fruit juices and certain types of wine, where the presence of diacetyl is undesired.

The invention relates to primers used for amplifying plecoptera insect mitochondrion COI (cytochrome c oxidase I) gene sequences, the plecoptera insect mitochondrion COI gene sequences are as shown in SEQ ID No.1 and SEQ ID No.2. The primers for amplifying are adopted, so as to amplify COI genes of various plecoptera species effectively, be conducive to accurately identifying close species difficult to indentify in some forms, and an important tool for classification and identification, phylogeny, population genetic structures and geographical population identification of plecoptera insects in China is provided.

The invention provides a reporter gene assay method by which the sex hormone-like activity inherent in a test substance can be accurately assayed excluding the effects caused by a decrease in cell activity (protein expressing capacity). The assay method is a reporter gene assay method using luciferase-expressing cells which comprises further tranferring a GFP gene into the luciferase-expressing cells, measuring the luciferase expression level and GFP expression level, and making a judgment about the thus-measured luciferase expression level using the decrease in GFP expression level as an indication of the decrease in cell activity.

The invention discloses a heat-resistant formate dehydrogenase gene, its coded polypeptide and a preparation method. Based on Bacillussp., the invention clones and isolates the heat-resistant formate dehydrogenase gene, which is useful for the preparation of It is useful to obtain heat-resistant formate dehydrogenase transgenic microorganisms, animals and plants, and recover the enzyme encoded by the gene. In addition, the present invention also provides the amino acid sequence and functional equivalent of the polypeptide having heat-resistant formate dehydrogenase activity. At the same time, the invention also provides a method for preparing, separating and purifying the polypeptide with heat-resistant formate dehydrogenase activity.

The present invention provides a modified bacterial strain capable of fermenting both hexose and pentose sugars for production of bioalcohol wherein a promoter of pyruvate dehydrogenase operon (PDH) is replaced with a promoter of a gene that is expressed under anaerobic conditions. The present invention further provides a method of obtaining modified bacterial strain capable of fermenting both hexose and pentose sugar for production of bioalcohol. The present invention also provides a method of fermenting lignocellulosic biomass having hexose and pentose sugar using the modified bacteria for production of biomass.

The invention relates to an ammonia transport protein gene, a protein coded by same and application thereof in the aspect of high-efficiency utilization of nitrogen of crops. The gene of the invention is derived from Dunaliella Salina and is called as DvAMT1 ;1. The gene has the function of transporting high-affinity ammonia transport proteins, can recover the capability of absorbing low-density NH<4+> of a yeast mutant and is indicated to have application value in the aspect of high-efficiency utilization of nitrogen of crops.

The invention relates to a detection kit for common campylobacter in foods and a detection method thereof, which is used for detecting and monitoring the microbial contamination of the foods. In the kit, 1mL of detection solution contains 10mM of Tris.Cl, 50mM of KCl, 25mM of MgCl2, 2.5mM of dNTP (dATP, dGTP, dCTP and dTTP) respectively, 5U/mu L of Taq DNA polymerase and 10muM of four kinds of common campylobacter primer pairs; the method adopts the virulence factor genes of the campylobacter to carry out detection, and can simultaneously differentiate four types when detecting the campylobacter; the detection time is only 1.5 days and is greatly shortened in comparison with the detection time of a traditional microbiology method; and the detection limit is below 15cfu/mL, and the campylobacter can be detected so long as the number of the campylobacter in bacterium liquid per milliliter is not less than 15. Moreover, the method has shorter detection time in comparison with the traditional method, is simpler and quicker, can save a large number of labour force and resources and is suitable for the requirement of quick detection.

The present invention is directed to KIAA1199, which is a novel factor involved in decomposition of hyaluronic acid, and to use thereof. More specifically, the invention is directed to a hyaluronic acid decomposition-promoting agent containing the KIAA1199 gene and a protein encoded by the gene; to a hyaluronic acid decomposition-inhibiting agent characterized by inhibiting the activity or expression thereof (including an siRNA or a monoclonal antibody); and to a method for screening a novel hyaluronic acid decomposition-controlling agent, in which the method contains employing the expression of KIAA1199 as an index.

This invention provides a host-vector complementation system, which permits selection of vector-carrying host cells, without requiring an antibiotic resistance gene. In some embodiments, this system utilizes a host which is guaB deficient and vectors that carry and express the guaB gene. The invention also discloses methods of making and using the system.

The invention discloses an engineering bacterium of beta-keto thiolase and 3-ketoacid coenzyme A transferase and its application, specifically a special-purpose expression engineering bacterium of beta-keto thiolase and 3-ketoacid coenzyme A transferase and its use in the production of acetoacetate. The engineering bacterium is obtained by leading the expression vector including the beta-keto thiolase and 3-ketoacid coenzyme A transferase in the Escherichia coli. The present invention also provides a preparation method of acetoacetate, which includes: fermenting the engineering bacterium expressing the beta-keto thiolase and 3-ketoacid coenzyme A transferase to obtain the beta-keto thiolase and 3-ketoacid coenzyme A transferase, and then adding substrate glucose and succinate solution, fermenting to obtain the acetoacetate. The special-purpose expression engineering bacterium of beta-keto thiolase and 3-ketoacid coenzyme A transferase of the present invention will play an important role in the industrialization production and wide application prospects.

The invention discloses an application of melatonin to alleviation of tomato pollen abortion due to high temperature induction. The application comprises the following steps: (1) melatonin and an additive are mixed in order to prepare an aqueous solution; (2) the aqueous solution is added into nutrient solutions for plant growth or soil or sprayed on plant leaf surfaces. The melatonin preparationcan be used for rapidly and efficiently improving response of tomato pollen to high temperature stress, in order to improve expression of genes related to autophagy, high temperature stress and antioxidant enzymes, improve capability for removing active oxygen in plant bodies, and improving vitality of plant pollen; the preparation technology is simple, usage is convenient, product and environmentpollution is not produced, phytotoxicity is not generated easily, and the application has wide application prospects.

The present invention relates to a composition for transfecting a cell with a genetic material comprising a first agent capable of directing the genetic material away from the acidic compartments in the cell and a second agent capable of stabilizing the microtubule or a network thereof. The invention also relates to the use of the composition in the manufacture of a medicament for treating a disease, a method for delivering a genetic material into a cell and a kit.

The invention discloses a method for screening human constitutive androstane receptor activators, particularly a method for quickly screening human constitutive androstane receptor activators by using a nano cerium oxide particle sensitizing agent. The method comprises the following steps: doping nano cerium oxide particles as a sensitizing agent into a rat-tail collagen solution coated cell culture plate, inoculating human hepatoma cell line HepG2 into the coated culture plate, and screening the human constitutive androstane receptor activators by using a routine report gene test. The method is simple and quick, has very high specificity, can be used for quickly screening whether industrial raw material chemicals, micromolecule chemicals, pesticides and other chemical substances contain the human constitutive androstane receptor activators, and has very high application value.

The invention discloses application of sucrose phosphorylase in preparation of 2-O-alpha-D-glucosyl-L-ascorbic acid. According to the application, a reaction system is formed by taking fermentation liquor, obtained through fermenting sucrose phosphorylase gene containing engineering bacteria, or broken liquor, obtained through resuspending wet thalli obtained after centrifuging the fermentation liquor with a buffer solution, as a catalyst, taking L-ascorbic acid as a substrate, taking sucrose as a cosubstrate and taking a buffer solution with a pH of 4.8 to 6.0 as a reaction medium, and a reaction is carried out at the temperature of 30 DEG C to 55 DEG C, thereby obtaining AA-2G. According to the application, the AA-2G is efficiently produced by employing the sucrose phosphorylase, the yield is 3.47g/L/h and is increased by 34.5% compared with that employing the existing reported B. longum derived sucrose phosphorylase, and the maximum concentration of the AA-2G can reach 178g/L; and recombinant bacillus subtilis can efficiently secrete and express the sucrose phosphorylase, and the fermentation liquor is directly applied to preparation of food-grade AA-2G.

The invention relates to a fluorescence analysis method based on HCR and cation exchange reaction of hydrogel, and the method comprises the following steps: modifying a C-C double bond on the surfaceof a substrate through a silanization reaction, and constructing a PEG hydrogel array on the substrate by means of a photomask through a free radical polymerization reaction initiated by ultraviolet light; opening a hairpin structure of a capture probe modified on the hydrogel by the target molecule, initiating a hybridization chain reaction between biotinylation hairpins H1 and H2, and forming along-chain double-chain DNA at a sensing site. Through biotin-streptavidin specific binding reaction, a biotinylated quantum dot CdS is fixed to a sensing site. CdS is initiated by Ag<+> to generate cation exchange reaction, and the fluorescence of Rhod-5N is greatly enhanced by Cd2<+>. Array point fluorescence is quantified by using a gene chip scanner, so that high-sensitivity and high-specificity analysis of target molecules is realized. The method is simple to operate, low in cost, high in sensitivity, good in specificity and high in universality.

The invention belongs to the field of biomedical materials and discloses application of miR-141 in the preparing of anti-osteoporosis and bone-resorption-inhibiting preparations. Experiments prove that the miR-141 inhibits the differentiation process of osteoclasts by regulating the expression of genes related to osteoclast differentiation. The miR-141 can inhibit bone mass loss and osteoporosis, so that the miR-141 can be used for preparing the various biomedical anti-osteoporosis and bone-resorption-inhibiting preparations.

The invention discloses a Fenton composite reagent used in a gene biological treatment system. The Fenton composite reagent comprises the following ingredients: 15-20 percent of FeSO4.7H2O, 3-5 percent of H2SO4, 2-3 percent of buffer agent and the balance of water, wherein the buffer agent is a weak acid, preferably acetic acid. The Fenton composite reagent mainly aims to synchronously meet the demands for (Fe<2+>) amount and PH which are required by Fenton reaction under the condition that sulfuric acid is not used independently, and has two main advantages as follows: (1) potential safety hazards of sulfuric acid are eliminated; (2) the operation is simple, and all that needed is to inject the reagent to meet the pH required by reaction.

The invention discloses a recombinant expression carrier containing a schistosoma japonicum gene which is a schistosoma japonicum thioredoxin peroxidase II gene. The invention also discloses a preparation method of the schistosoma japonicum thioredoxin peroxidase II and the application of the recombinant expression carrier. The recombinant expression carrier containing the schistosoma japonicum gene can highly express the schistosoma japonicum thioredoxin peroxidase II, and the highly expressed recombinant schistosoma japonicum thioredoxin peroxidase II can induce mouse to generate the protection function of resisting the infection of schistosoma japonicum at a certain level.

The present invention belongs to the field of biology technology, and is method of increasing the TIAs content in hairy root of catharantus roseus. The present invention includes cloning str and g10h genes from catharantus roseus, constructing plant expression vector of str and g10h genes, genetically transforming to catharantus roseus to obtain transgenic catharantus roseus hairy root, determining the TIAs content in hairy root of catharantus roseus in high efficiency liquid chromatography process, and determining the expression of biological synthesized gene of TIAs in hairy root of catharantus roseus via fluorometric quantitative PCR. The transgenic catharantus roseus hairy root has obviously raised TIAs content, especially 234 times raisedVinblastine content. The present invention provides one new method of raising TIAs content in hairy root of catharantus roseus and producing Vinblastine and Vincristine as important clinical anticancer medicine.

The invention relates to usage of 3-carbonyl-12-alkene-ursane compound for inhibiting gastric cancer cell SGC-7901 and hepatoma cancer cell BEL-7404. Experiment shows that the 3-carbonyl-12-alkene-ursane compound is effective to inhibit cell proliferation of the gastric cancer cell SGC-7901 and the hepatoma cancer cell BEL-7404 and can be used for preparing antitumor medicine. Electrophoretic detection shows that the 3-carbonyl-12-alkene-ursane compound can accelerate apoptosis of the hepatoma cancer cell BEL-7404 by accelerating increase of apoptosis relative gene Bax expression.