42 results about "Nucleic acid" patented technology

This invention provides methods for massive parallel nucleic acid analysis. A closed complex of nucleic acid template, nucleotide and polymerase can be formed during polymerase reaction, absent divalent metal ion. This is used to trap the nucleotide complementary to the next template nucleotide in the closed complex. Detection of the trapped nucleotide allows determination of the sequence of this next correct nucleotide. In this way, sequential nucleotides of a nucleic acid template can be identified, effectively determining the sequence. This method is applied to sequence multiple templates in parallel, particularly if they are immobilized on a solid support.

The present disclosure relates to methods for isolating, amplifying, and/or analyzing nucleic acids in the presence of an anion exchange material by performing the isolation, amplification and/or analysis step in the presence of at least one anionic compound.

A method for authenticating a product where the product is composed of a plurality of components is described. The method for authenticating the product comprises providing a liquid compound, e.g. paint, that comprises at least one invisible market, e.g. nucleic acid, in which the compound is associated with a first entity. The method then proceeds to apply the compound to the surface of the product at the juncture of at least two components. The method also comprises associating the application of the compound to the at least two components with the first entity.

The present invention relates to methods and devices for identifying and quantifying, including low abundance, nucleotide base mutations, insertions, deletions, translocations, splice variants, miRNA variants, alternative transcripts, alternative start sites, alternative coding sequences, alternative non-coding sequences, alternative splicings, exon insertions, exon deletions, intron insertions, or other rearrangement at the genome level and/or methylated nucleotide bases.

A method for detecting human papillomaviruses (HPV) in biological material is described, in which method nucleic acids are extracted/isolated from biological material and HPV-specific DNA is detected in the isolated nucleic acids. This method uses at least one of the nucleotide sequences SEQ ID No. 1 or SEQ ID No. 2 from the enclosed sequence listing or sequences which bind to sequences to which one of the sequences SEQ ID No. 1 or SEQ ID No. 2 binds or sequences which are complementary to the abovementioned sequences.

The invention provides methods and compositions for detecting a change in a nucleic acid polymerase conformation involving contacting a nucleic acid polymerase non-covalently attached to a single walled carbon nanotube (SWNT) with a first nucleotide or first nucleotide analog and a template and detecting the conformationally changed nucleic acid polymerase by measuring a first electrical conductance change in the SWNT between the nucleic acid polymerase and the conformationally changed nucleic acid polymerase. The method is useful for sequencing of polynucleotides.

The present invention relates to a method of isolating cells from a sample which method comprises binding said cells to a solid support by means of a non-specific ligand immobilised on said solid support, particularly to a method of isolating microorganisms from a sample. Preferred ligands for use in such methods include carbohydrates and derivatives thereof. Also described is a kit for isolating microorganisms from a sample comprising: (a) a solid support having immobilised thereon a ligand which is capable of non-specific binding to microorganisms; (b) means for binding microorganisms to said solid support; optionally (c) means for lysing said cells; and optionally (d) means for binding nucleic acid released from said lysed cells to a solid support.

The invention relates to the technical field of biosensor, in particular to a fluorescence biosensor based on amplification of a hybridization chain reaction. The problems that the specificity and sensitivity of the method for detecting the kanamycin is low, and the cost is high in the above prior art are solved. According to the fluorescence biosensor based on the amplification of the hybridization chain reaction, the cyclic amplification effect is achieved through the hybridization chain reaction between the endonuclease IV and the chain. The mixture is reacted homogeneously by fluorescenceresonance energy transfer of the fluorophores and the quenching group. The preparation method comprises the following steps of: preparing gold nanoparticles; modifying Walker and Track to the surfaceof gold nanoparticles; mixing the labeled gold nano solution with the homogeneous reaction solution; performing hybridization chain reaction, and detecting the fluorescence; using the specific recognition of the nucleic acid Aptamer, using the nucleic acid Aptamer for high specific detection of the target kanamycin; and using the amplification of the hybridization chain reaction to achieve signalamplification.

The invention discloses a chromium-based functional nucleic acid sensor and an application thereof in the technical field of metal ion detection. The sensor comprises a molecule recognition element, asignal amplification element and a signal conversion element, wherein the molecule recognition element comprises chromium ion deoxyribozyme consisting of a substrate chain and an enzyme chain; the signal amplification element comprises an isothermal amplification system and hemin, and the isothermal amplification system comprises an amplification template; the signal conversion element comprisesa color developing agent. The sensor is based on the chromium ion deoxyribozyme, an isothermal index amplification reaction and a G-quadruplex liquid phase sensing technology, is convenient, quick, high in sensitivity, high in specificity and resistant to high salinity in chromium ion detection and can be used in field detection of chromium ions in the environment.

The invention discloses a high-salinity-resistant nucleic acid sensor for copper and application thereof. The sensor comprises a molecular recognition element, a signal multiplication element and a signal conversion element, wherein the molecular recognition element comprises copper ion deoxyribozyme; the copper ion deoxyribozyme consists of a substrate chain and an enzyme chain; the signal multiplication element comprises an isothermal amplification system; the isothermal amplification system comprises an amplification template; the signal conversion element comprises hemin and a color developing agent. The sensor provided by the invention is used for specifically recognizing a copper ion; the primary enlargement and conversion of a signal are carried out through an isothermal index multiplication reaction; a G-four-chain body structure with activity is formed under the induction of the hemin; the color developing agent is catalyzed to develop a color, thus, secondary multiplication and conversion are generated; the signal is converted into a visual signal, and the qualitative and quantitative detection in a high-salinity environment can be carried out.

The invention relates to a method for preparing composite yeast nutritious drinks, and belongs to the technical field of nutritious drinks. According to the technical scheme, the method includes the steps that lyceum barbarum extract is prepared; a yeast extract solution is prepared; syrup feed liquid is prepared; the obtained lyceum barbarum extract and the yeast extract solution are added to the syrup feed liquid, the lyceum barbarum extract, the yeast extract solution and the liquid are evenly mixed, sodium erythorbate is added to the mixed solution, and high pressure homogenization is conducted on the mixed solution; the solution after high pressure homogenization processing is sterilized, filtered and clarified through a ceramic membrane, and filled into vessels, and then the composite yeast nutritious drinks are prepared. The prepared lyceum barbarum and yeast nutritious drinks have quite rich nutrient substances, lyceum barbarum contains a lot of nutrients such as amino acids, lyceum barbarum polysaccharides, inorganic salt, glucose, taurine and betaine, and the nutrients are essential to human body; yeast extract contains 18 kinds of amino acids and polypeptides, multiple B vitamins, nucleic acids and multiple mineral elements. The prepared drinks have the functions of nourishing the kidney and tonifying the liver, benefiting qi and promoting the production of body fluid, improving eyesight and soothing nerves, resisting fatigue and improving human body immunity.

A method for the accurate quantification of a virus in a sample, by reverse transcription polymerase chain reaction (RT-PCR), includes: adding a known concentration of a Mengo virus to the sample as control for the nucleic acids extraction step, the Mengo virus being a mutant strain with the same growth properties than those of the wild-type Mengo virus, and with non-pathogenic capacity; performing a nucleic acids extraction to obtain a nucleic acids suspension; analyzing the nucleic acids suspension by RT-PCR with primers and probes; quantifying the amplimers resulting from the RT-PCR; determining the concentration of the virus in the sample by comparison of the value obtained with an appropriate standard curve; and determining the concentration of the Mengo virus by comparison of the value obtained with an appropriate standard curve.

The invention discloses one group of nucleic acid aptamers capable of specifically bonding with tuberculosis mycobacterium Beijing genotype strain surface lipolysaccharide namely mannose modified mannosylated lipoarabinomannan (ManLAM) and application of the nucleic acid aptamers. The aptamers are specific to small-molecular single-chain DNA (ssDNA) of the ManLAM, and have a nucleotide sequence as shown in the SEQ ID No.1-5. The ssDNA has functions similar to those of a monoclonal antibody, and can be directly and specifically bonded with ManLAM. By adopting an enzyme-linked oligonucleotide assay (ELONA) method, the ManLAM antigen in serum of patients with pulmonary tuberculosis, extrapulmanory tuberculosis and the like can be quickly effectively detected in 2 hours, the ManLAM antigen in sputum of an active pulmonary tuberculosis patient can be directly identified, and the detection sensitivity on the Man LAM antigen can be 0.035mu g/mL. The aptamers are simple to prepare and low in cost, and can be used as an effective novel reagent for diagnosing the mycobacterium tuberculosis antigen of early and latent tuberculosis.

Described herein are methods for making and using magnetic ionic liquid that have at least one cationic component and at least one anionic component, where at least one of the cationic components or the anionic components is a paramagnetic component. The magnetic ionic liquids are capable of manipulation by an external magnetic field.

The present invention provides methods for producing a transgenic soybean plant, soybean plant part, or soybean plant cell having increased resistance to soybean cyst nematode parasitism, comprising introducing into the soybean plant, soybean plant part, or soybean plant cell, a heterologous miR164 nucleotide sequence, wherein the heterologous miR164 nucleotide sequence is expressed in the soybean plant, soybean plant part or soybean plant cell, thereby producing a transgenic soybean plant, soybean plant part, or soybean plant cell having increased resistance to soybean cyst nematode parasitism relative to a control soybean plant, soybean plant part, or soybean plant cell. The invention further provides methods of reducing soybean cyst nematode cyst formation in a soybean plant, soybean plant part, or soybean plant cell as well as compositions including nucleic acid constructs for transforming a plant, plant part or plant cell and transformed plants, plant parts and plant cells.

Antisense compounds, compositions and methods are provided for modulating the expression of mucin 1, transmembrane. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding mucin 1, transmembrane. Methods of using these compounds for modulation of mucin 1, transmembrane expression and for treatment of diseases associated with expression of mucin 1, transmembrane are provided.

A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.

A pharmaceutical composition for treating cancer, containing a crRNA and an endonuclease as active ingredients is disclosed. The composition can be customized according to the needs of patients or cell types by specifically treating cancer cells on the basis of specific binding properties of DNA and RNA. The nuclease activity of a CRISPR PLUS system, containing both an endonuclease and an exonuclease can be activated by means of the binding between crRNA and a gene specifically found in cancer cells. Therefore, the cancer treatment effect of the composition is more specific than that of other anti-cancer agents that have been developed up till now.

The invention provides a nucleic acid combination, a kit and a method for detecting cryptosporidium oocysts, and relates to the field of biotechnology. The nucleic acid combination comprises an upstream primer shown as SEQ ID NO.1, a downstream primer shown as SEQ ID NO.2 and a fluorescent probe shown as SEQ ID NO.3. The method for detecting the cryptosporidium oocysts comprises the step of performing an RPA reaction by using the nucleic acid combination. The nucleic acid combination can fast, specifically and sensitively detect the cryptosporidium oocysts in a sample by an RPA technology. By the method, the temperature adjustment is not needed, the detection time is short, the detection limit is low, the specificity is high, and no false positive exists.