49 results about "Nucleotide" patented technology

A method for detecting human papillomaviruses (HPV) in biological material is described, in which method nucleic acids are extracted/isolated from biological material and HPV-specific DNA is detected in the isolated nucleic acids. This method uses at least one of the nucleotide sequences SEQ ID No. 1 or SEQ ID No. 2 from the enclosed sequence listing or sequences which bind to sequences to which one of the sequences SEQ ID No. 1 or SEQ ID No. 2 binds or sequences which are complementary to the abovementioned sequences.

The invention provides methods and compositions for detecting a change in a nucleic acid polymerase conformation involving contacting a nucleic acid polymerase non-covalently attached to a single walled carbon nanotube (SWNT) with a first nucleotide or first nucleotide analog and a template and detecting the conformationally changed nucleic acid polymerase by measuring a first electrical conductance change in the SWNT between the nucleic acid polymerase and the conformationally changed nucleic acid polymerase. The method is useful for sequencing of polynucleotides.

The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to novel human secreted IGFBP-like polypeptides. Other aspects of the invention include vectors containing processes for producing novel human secreted IGFBP-like polypeptides, compositions, and methods of use for such polypeptides including in cancer.

The invention specifically discloses a tea tree MYB transcription factor CsAN1 and an application thereof in regulation of anthocyanin metabolism. A nucleotide sequence of the tea tree MYB transcription factor CsAN1 is represented as SEQ ID NO:1. CsAN1 is connected with a 35S promoter and then is connected to a pBI121 carrier, meditated transformation of tobaccos is realized by agrobacteria GV3101, meanwhile, the gene as well as genes CsGL3 and CsTTG1 capable of forming compounds with the gene is used for transforming the tobaccos respectively, leaves of overexpressed tobacco plants turn red, anthocyanin content detected by a spectrophotometer is remarkably increased, and two main pigments including cyanidine and delphinidin are detected through HPLC (high performance liquid chromatography).

The invention belongs to the field of microbial genetic engineering, and discloses a GTP cyclohydrolase I gene folE and application. A nucleotide sequence of the GTP cyclohydrolase I gene folE is shown as SEQ ID NO:1. The gene is derived from food-borne lactobacillus plantarum, is safe and can be used in the field of later-stage food fermentation. A gene knockout strain screening method can be used for improving the screening efficiency of knockout strains. The key role of gene folE gene in folic acid synthesis is proved, and a certain theoretical basis is provided for the research and development of folic acid synthesis functional food. The GTP cyclohydrolase I gene folE and application analyze that the gene is derived from food-grade microorganisms and is safe; and the GTP cyclohydrolaseI gene folE and application analyze that the gene folE gene has nothing to do with cell morphology and strain growth except playing an important role in folic acid synthesis.

The invention discloses an HCV (hepatitis C virus) core antigen, which is a polypeptide encoded by a DNA (deoxyribonucleic acid) of which the amino acid sequence is shown in SEQ ID NO.1 and the nucleotide sequence is shown in SEQ ID NO.2. The invention also discloses a hybridoma cell line secreting monoclonal antibodies, the hybridoma cell line is named as SC16-H6 and has been preserved in the China General Microbiological Culture Collection Center on July 14th, 2011, and the preservation number is CGMCC No. 5074. The invention also discloses another hybridoma cell line secreting monoclonal antibodies, the hybridoma cell line is named as SC23-G5 and has been preserved in the China General Microbiological Culture Collection Center on July 14th, 2011, and the preservation number is CGMCC No. 5075. The invention also discloses a monoclonal antibody of an anti-HCV core antigen, which is secreted by the hybridoma cell line (preservation number: CGMCC No. 5074) or the hybridoma cell line (preservation number: CGMCC No. 5075), and has a specificity to the polypeptide shown in SEQ ID No.1.

Disclosed is a process for producing the L-form of an amino acid enzymatically from an enantiomeric mixture of the amino acid. The process includes the step of contacting a transformant or a treated product thereof with an enantiomeric mixture of the amino acid. The transformant includes a single host and one or more recombinant vectors introduced thereinto, in which the one or more recombinant vectors include one or more expression vectors and nucleotide sequences integrated thereinto respectively, and the nucleotide sequences are a nucleotide sequence encoding an enzyme capable of converting the D-form of an amino acid into a keto acid and a nucleotide sequence encoding an enzyme capable of converting a keto acid into the L-form of an amino acid. According to this process, a reaction for converting the D-form of an amino acid into a keto acid and a reaction for converting the keto acid into the L-form of the amino acid can be conducted in a single step. Disclosed also is a transformant capable of converting into the L-form of an amino acid as accumulated in a high concentration. The L-form of an amino acid can be efficiently produced from an inexpensive starting material by using the transformant.

The invention discloses a rice secretary type thioredoxin gene, coded proteins thereof and application thereof and aims to provide the rice secretary type thioredoxin gene, the coded proteins thereof and a method for improving antioxidant activities of plants and delaying plant senescence by using the gene. The nucleotide sequence of the rice secretary type thioredoxin gene provided by the invention is a No.1 DNA sequence in a sequence table; and the amino acid sequence coded by the secretary type thioredoxin gene is a No.2 amino acid residue sequence in the sequence table. Experiments show that when the gene is excessively expressed in the rice, hydrogen peroxide content of a plant can be reduced and chlorophyll content can be improved in a normal state or in the presence of salts, so a rice senescence process is delayed. The rice secretary type thioredoxin gene, the coded proteins thereof and the application thereof have important application in aspects of improving the anti-oxidation and anti-aging performance of rice by using molecular breeding.

The invention discloses one group of nucleic acid aptamers capable of specifically bonding with tuberculosis mycobacterium Beijing genotype strain surface lipolysaccharide namely mannose modified mannosylated lipoarabinomannan (ManLAM) and application of the nucleic acid aptamers. The aptamers are specific to small-molecular single-chain DNA (ssDNA) of the ManLAM, and have a nucleotide sequence as shown in the SEQ ID No.1-5. The ssDNA has functions similar to those of a monoclonal antibody, and can be directly and specifically bonded with ManLAM. By adopting an enzyme-linked oligonucleotide assay (ELONA) method, the ManLAM antigen in serum of patients with pulmonary tuberculosis, extrapulmanory tuberculosis and the like can be quickly effectively detected in 2 hours, the ManLAM antigen in sputum of an active pulmonary tuberculosis patient can be directly identified, and the detection sensitivity on the Man LAM antigen can be 0.035mu g/mL. The aptamers are simple to prepare and low in cost, and can be used as an effective novel reagent for diagnosing the mycobacterium tuberculosis antigen of early and latent tuberculosis.

The invention belongs to the technical field of molecular biology and discloses a molecular marker for identifying rice grain length characters, an identification method and application. The molecular marker is GS3-TPAP and comprises GS3-TPAP-O-F with the nucleotide sequence shown as SEQ ID NO.1, GS3-TPAP-O-R with the nucleotide sequence shown as SEQ ID NO.2, GS3-TPAP-I-F with the nucleotide sequence shown as SEQ ID NO.3, and GS3-TPAP-I-R with the nucleotide sequence shown as SEQ ID NO.4. The molecular marker can be used for directly identifying two allelotypes of the rice grain length characters of the GS3 gene, the accuracy reaches 100%, the two allelotypes can be distinguished well, influences of environmental conditions are avoided, and the identifying method is low in consumption and suitable for large-scale application and popularization.

The present invention discloses diamondback moth cecropin 3, a preparation method and an application thereof, wherein the diamondback moth cecropin 3 gene has a nucleotide sequence represented by SEQIDNO:1-2 and has a corresponding amino acid sequence represented by SEQIDNO:3, and sequence analysis results show that the mature peptide of the diamondback moth cecropin 3 has an amino acid sequence represented by SEQIDNO:4. According to the present invention, the diamondback moth cecropin 3 gene or the mature peptide sequence thereof is subjected to prokaryotic expression to obtain the diamondback moth cecropin 3, and the obtained diamondback moth cecropin 3 provides strong inhibition effects for gram-negative bacteria and gram-positive bacteria, provides strong inhibition effects for pathogenic fungi, especially provides strong inhibition effects for a lot of pathogenic fungi of melons and fruits, and is expected to be a novel peptide antibiotic.

The invention discloses a method for determining correlation between single nucleotide polymorphisms rs4840568 and rs13277113 of Han people in Chinese Mainland and systemic lupus erythematosus (SLE). The method comprises that through a non-labeled probe high-resolution solubility curve method, a SLE patient is subjected to genetic typing so that the correlation between single nucleotide polymorphisms rs4840568 and rs13277113 of Han people in Chinese Mainland and SLE is determined. At present, it is proved that environmental factors and gene factors produce important effects in SLE generation and development and thus a research result obtained by the method provides a theoretical basis for in-depth knowledge of SLE diseases.

The invention belongs to the technical field of biology and in particular relates to an absolute fluorescence quantitative PCR (Polymerase Chain Reaction) primer and a kit for detecting an atypical porcine pestivirus (APPV). Nucleotide sequences of the absolute fluorescence quantitative PCR primer for detecting the atypical porcine pestivirus are shown as SEQ ID NO. 1 and SEQ ID NO. 2; the primer can be used for specifically amplifying an NS3 gene segment of the atypical porcine pestivirus. The invention further provides the kit for detecting the atypical porcine pestivirus and a method for detecting the atypical porcine pestivirus; the kit has the beneficial effects of rapidness and high efficiency, convenience for operation, high specificity, high sensitivity, simplicity for identification, low cost and the like. The primer, the kit and the method, provided by the invention, are used for carrying out quantitative detection and determination on the atypical porcine pestivirus and can be used for rapidly and accurately detecting the copy number of targeting genes and a technological foundation is provided for detecting and identifying the APPV.

Provided in the present invention are a molecule for biomarking cancers and a test composition for screening cancers and a detection method thereof comprising designing a number of oligonucleotide primers or probes by analyzing specimens for methylated regions of targeted genes PAX1, ZNF582, SOX1 and NKX6-1 in physical examination, and then using the oligonucleotide probes to detect whether methylation exists in the target genes, and further judge the likelihood of the occurrence of cancer. The detection methods for methylation status include methylation-specific PCR, (MSP), quantitative methylation-specific PCR (QMSP), bisulfate sequencing (BS), microarrays, mass spectrometer, denaturing high-performance liquid chromatography (DHPLC), pyrosequencing, and enzyme-linked immunosorbent assay (ELISA).

The invention belongs to the field of biological medicines, and particularly relates to a kit for detecting human herpes virus infection and a detection method thereof. The invention firstly disclosesa kit for simultaneously detecting multiple herpes viruses, the kit comprises a primer probe system for detecting human herpes virus infection, and the primer probe system comprises a nucleotide sequence group as shown in SEQ ID NO: 1-21. When the kit is used for herpes virus detection, the detection period is shortened to 3-4 hours, and clinically common virus infection can be rapidly, efficiently and accurately determined. The detection method provided by the invention can be used for rapidly monitoring the copy number change of the specific virus in the body of the virus infected patient in real time, evaluating in time and providing adjuvant therapy suggestions for clinicians. Compared with the traditional multiple PCR scheme, the efficient multiple multicolor digital PCR experimentalscheme disclosed by the invention has the advantage that the detection method is more efficient and accurate.

The invention discloses a molecular marker of maize transcription factor ZmPIF3 transgenic rice and application of the molecular marker and belongs to the field of crop genetic breeding. A maize transcription factor ZmPIF3 transgenic rice material which is obtained already has obvious water-saving drought-enduring phenotype. According to a nucleotide sequence of maize transcription factor ZmPIF3 gene, a primer of the molecular marker is designed in CDS, PCR technology is applied to acquire DNA segments of ZmPIF3 and sequence the DNA segments, and the primer of the molecular marker is obtained according to specific segment screening of ZmPIF3. The molecular marker is high in repeatability, stable in amplification, simple and convenient to operate and low in cost and can be used for detecting maize ZmPIF3 genes in rice background, and a solid foundation is laid for molecular marker assisted selection in the future by utilizing ZmPIF3 gene transgenic rice.

The invention provides a single-chain antibody capable of specifically recognizing Reg3A protein, including the nucleotide sequence and amino acid sequence of the heavy and light chain variable regions encoding the protein, and the CDR region determining the specificity of the antibody. In the present invention, a high-affinity single-chain antibody targeting Reg3A protein is screened out through a phage antibody library, and then the Reg3A single-chain antibody is obtained through construction, expression, and purification. The Reg3A single-chain antibody of the present invention can specifically bind to Reg3A, so it can treat or detect diseases related to excessive and abnormal expression of Reg3A.

The invention relates to a kit of a peripheral blood free-nucleotide miRNAs-based ultrasensitive detection device and a detection method of the kit. The kit comprises 1) a microarray detection pool; 2) an external magnetic field; 3) premixed reaction solutions A and 4) premixed reaction solutions B. During detection of multiple targets to be detected, the premixed reaction solutions A for respectively and correspondingly detecting the targets to be detected are configured in parallel and are respectively marked as a premixed reaction solution A1 and a premixed reaction solution A2. The kit iscombined with biomolecular diagnosis, nano-magnetic microsphere enrichment and biochip technologies to develop a novel technique capable of directly and accurately detecting peripheral blood miRNAs related to occurrence, development and drug resistance of a malignant tumor without amplification at high sensitivity and high flux. The kit has an important value in the aspects of noninvasive detection of medical tumors, personalized medicine application, survival after chemotherapy and the like.