Method for determining correlation between single nucleotide polymorphisms rs4840568 and rs13277113 of Han people in Chinese Mainland and systemic lupus erythematosus (SLE)
A single nucleotide polymorphism and correlation technology, applied in the field of genetic engineering
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[0016] 1. Research object
[0017] 286 SLE patients (male 26 persons, 260 females, median age 29 years, age range 12-55 years) and 352 ethnically matched healthy controls (30 males, 322 females, median age 28 years, age range 17- 46 years old) participated in the study. All the healthy control group had no family history of SLE and related clinical symptoms. This study was approved by the Ethics Committee of Shenzhen Hospital and informed consent was obtained from all participants.
[0018] 2. Genotyping
[0019] Using Innogent's whole genome DNA extraction kit (Innogent, China), the collected peripheral blood genomic DNA was extracted according to the instructions of the kit. The genotype of the samples was identified by the high-resolution melting curve method of unlabeled probes. The method is briefly described as follows: first, asymmetric PCR was carried out, and the 20 mL system contained 20 ng of genomic DNA template, 1′PCR buffer solution (Takara, Japan), 200 mM dNTP
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