149results about "Bacteria" patented technology

The invention overcomes the deficiencies of the prior art by providing a rapid approach for isolating binding proteins capable of binding small molecules and peptides. In the technique, libraries of candidate binding proteins, such as antibody sequences, are expressed in the periplasm of gram negative bacteria and mixed with a labeled ligand. In clones expressing recombinant polypeptides with affinity for the ligand, the concentration of the labeled ligand bound to the binding protein is increased and allows the cells to be isolated from the rest of the library. Where fluorescent labeling of the target ligand is used, cells may be isolated by fluorescence activated cell sorting (FACS). The approach is more rapid than prior art methods and avoids problems associated with the outer surface-expression of ligand fusion proteins employed with phage display.

The invention discloses a solid microbial agent used for remedying the soil contaminated by petroleum, belonging to the technical field of bioremediation of soil contaminants. First-class seed liquid of acinetobacter radioresistens and rhodococcus erythropolis are mixed in equal parts after two steps of fermentation in liquid and solid states and cultivation to obtain the solid microbial agent, wherein, the number of effective viable bacteria of acinetobacter radioresistens and rhodococcus erythropolis is 10 to 10 CFU/g. The solid microbial agent in the invention has the advantages of low cost, high number of viable bacteria; the invention further has the advantages that micro-ecological environment improvement of the soil can be improved and secondary contamination cannot be caused to the environment, thus applicable to the bioremediation of the soil which is contaminated by petroleum and petroleum products.

The invention discloses an alcaligenes faecalis and application thereof. The alcaligenes faecalis is named alcaligenes faecalis (Alcaligenesfaecalis) NR and is stored in the China General Microbiological Culture Collection Center (CGMCC) with CGMCCNo.7543 on April 28, 2013. The alcaligenes faecalis is applicable to the treatment of nitrogen-containing organic wastewater and can be used for synchronously removing nitrogen and organic carbon in the wastewater. The alcaligenesfaecalis NR has both functions of denitriding and removing the organic carbon synchronously, only needs one aerobic stage to realize the synchronous removal of the organic carbon and nitrogen, and has the removal rate of about 80 to 90% for the organic ratio, and the removal rate of about 78 to 88% for TN (Total Nitrogen).

The present invention relates to a biological control strain AR156 for preventing and treating root-knot nematodes of greenhouse vegetable, belonging to plant protection technical field. The bacteria is bacillus sp., which is cultivated in PDA culturing liquor under 37 degree and 180rpm, then is centrifuged 10min under 6000rpm. Picking wet bacteria and bacteria storage liquid is prepared into bacteria agent according to 1:40 (g/ml)proportion. The living bacteria concentration is 1*109-1*1012cfu/ml.The effective rate of the bacteria agent amount to 58-75% for preventing root-knot nematodes of cucumber, towel gourd, tomato etc, and yield increase more than 30-50%. The bacteria agent can also treat wilt, epidemic disease and soil hardening. The bacteria agent can be sprayed, composted and irrigated to root.

The invention discloses pseudomonas stutzeri and its culture, immobilization and use. The disclosed pseudomonas stutzeri is pseudomonas stutzeri WZUF25, is preserved in the China general microbiological culture collection center and has the preservation number of CGMCC NO. 7685. The pseudomonas stutzeri has wide aerobic denitrification suitable pH and temperature ranges, has a high NO3<->-N and NO2<->-N removal rate and a high NH4<->-N assimilation capability, and can provide a culture source for synchronous nitrification and denitrification. The invention also provides immobilized pseudomonas stutzeri prepared by an active carbon-calcium alginate embedding method. The immobilized pseudomonas stutzeri has a good NO3<->-N removal capability and good stability.

The invention discloses bacillus subtilis and an application thereof. The accession number of the bacillus subtilis BLG010 in the China General Microbiological Culture Collection Center is CGMCC No.5953. The bacillus subtilis BLG010 with number being CGMCC No.5953 can effectively inhibit the growth of fusarium oxysporum f. sp. cubense at low concentration and has great significance on control of banana wilt.

A membrane supported bioreactor arrangement and method for anaerobic conversion of gas into liquid products including membrane modules having hollow fibers packed across a cross sectional area of the membrane module, each of the hollow fibers formed from an asymmetric membrane wall having a porous outer layer defining biopores for retaining a porous biolayer about the outer surface of the membrane wall and a less permeable hydration layer around the hollow fiber lumen; a membrane vessel for surrounding the outside of the hollow fibers with a process gas from a gas supply conduit; and a liquid supply conduit operably connected to the hollow fibers for supplying a process liquid to the hollow fiber lumens. The gas supply conduit enables the formation of a biolayer on the outer surface of the hollow fiber wall by interaction of microorganisms with the process gas and the production of a liquid product.

The present invention relates to a safe non-virulent Yersinia enterocolitica mutant strain for delivering heterologous proteins in target cells carrying mutations in at least one of the effector genes yopH, yopO, yopP, yopE, yopM, yopT genes and at least one additional mutation in the invasin genes chosen from yadA and/or inv. The present invention also relates to a safe non-virulent Yersinia enterocolitica mutant strain for delivering heterologous proteins in target cells according to claim 1 carrying mutations in all effector genes yopH, yopO, yopP, yopE, yopM, yopT genes and at least one additional mutation in the invasin genes chosen from yadA and/or inv. The present invention also relates to an expression vector for delivering a heterologous protein into a target cell using a Yersinia enterocolitica mutant strain according to any of the claims 1 to 4, which comprises in the 5' to 3' direction :(a) a promoter of a Yersinia virulon gene, (b) a first DNA sequence encoding a delivery signal from a Yersinia effector protein, operably linked to said promoter; and, (c) a second DNA sequence coding for said heterologous protein, fused in frame to the 3' end of said first DNA sequence. The present invention further relates to methods and compositions comprising (the use of) the afore-mentioned mutant strains and expression vectors.

The invention discloses denitrifying phosphorus removal bacteria bacillus cereus H-hrb01, and belongs to the technical fields of biological engineering and environmental engineering, wherein bacillus cereus H-hrb01 belongs to bacillus genus by identification and is conserved in the general microbiology centre of the China committee for culture collection of microorganisms, the conservation date is March 27th 2012, and the conservation number is CGMCC NO.5939; bacillus cereus H-hrb01 can take various organic matters as carbon sources, has good denitrifying and phosphorus-removing efficiency, and can be used for sewage biological denitrification and dephosphorization process; bacillus cereus H-hrb01 is activated and cultivated, and fed into artificial wastewater to mix and then introduced into an SBR (styrene butadiene rubber) biofortification device, and each effluent index of the reactor is superior to that of a contrast system which is not fed with the bacterial strain. The removal rates of the COD (chemical oxygen demand), ammonia nitrogen and dissoluble phosphorus in the sewage are respectively up to 93.90%, 97.70% and 96.25%.

The present invention discloses a high-yield strain of high temperature resistant 1,4-beta-D-xylanase, and a method for producing the high temperature resistant 1,4-beta-D-xylanase through fermentation of the high-yield strain, and provides partial enzymatic properties of the high temperature resistant 1,4-beta-D-xylanase. After the strain identification, the high-yield strain is named Paenibacillus campinasensis G1-1, and is preserved in the China general microbiological culture collection center. The preservation number of the strain is CGMCC No. 5023. According to the present invention, the high temperature resistant 1,4-beta-D-xylanase produced through the fermentation of the high-yield strain is subjected to separation and purification to obtain a single component, wherein the single component has a relative molecular weight of 41.3 KDa, an optimal operation temperature of 60 DEG C and an optimal operation pH of 7.0; the relatively stable enzyme activity of the high temperature resistant 1,4-beta-D-xylanase is remained in the temperature range of 40-70 DEG C and the pH range of 5.0-9.0; the Paenibacillus campinasensis G1-1 provided by the present invention is applicable for a plurality of fields such as paper making, food, feedstuff and the like, and has a broad application prospect.

The invention belongs to the technical field of animal infectious disease prevention and curing, and particularly relates to application of mycoplasma bovis MbovP730 protein in natural infection and vaccine immunity identification. According to the codon preference of escherichia coli, the Mbov_730 gene of M.bovis HB0801 is cloned and is modified, and a mycoplasma bovis tryptophan codon UGA is mutated into a tryptophan codon UGG in the escherichia coli so as to express recombinant mycoplasma bovis MbovP730 protein in the escherichia coli. The nucleotide sequence of the MbovP730 protein is disclosed in SEQ ID NO: 13, and the protein can be used as the antigen protein to be applied to the preparation of a kit for identifying mycoplasma bovis wild strain infection and vaccine strain immune identification.

The present invention provides Fibroblast Growth Factor-Like (FGF-L) polypeptides and nucleic acid molecules encoding the same. The invention also provides selective binding agents, vectors, host cells, and methods for producing FGF-L polypeptides. The invention further provides pharmaceutical compositions and methods for the diagnosis, treatment, amelioration, and/or prevention of diseases, disorders, and conditions associated with FGF-L polypeptides.

The invention discloses a Brevibacillus laterosporu with function in quickly decomposing nitrite nitrogen and bacteriostasis function and an application thereof. The screening strain is Brevibacillus laterosporu YB-02, the strain collection number is CGMCC-No. 9757. The strain provided by the invention has a capacity of effectively decomposing nitrite nitrogen in water, has cholate resistance and trypsin resistance, and further has higher inhibiting effects to mariculture pathogenic bacteria like vibrio splendidus, shewanella smarisflavi and edwardsiella tarda. Therefore, the strain provided by the invention can be used for developing probiotics products for mariculture water purification and bacterial inhibition, the antibiotics abuse is avoided, the fishing disease is prevented and treated effectively, the quality and yield of marine food products are improved; the strain has effective, fast and safe water purification effect to acute water pollution, and therefore, the strain is wide in application prospect.

The invention discloses Escherichia coli and a method for preparing L-cysteine by using the same. Escherichia coli BL21(DE3) is used as a template for the genome of the Escherichia coli, and the template has serine acetyltransferase shown as a sequence in SEQ ID NO.1 and in deletion of a gene metC, a gene tnaA, a gene gshA and a gene dfp. The method comprises the following steps of: firstly, putting the Escherichia coli in a seed culture medium for culturing; and then putting the Escherichia coli in a fermentation culture medium to ferment to obtain the L-cysteine. The invention has the advantages that by utilizing the Escherichia coli to prepare the L-cysteine, the conversion rate of a substrate and the output of the L-cysteine are greatly improved; the conversion rate of the substrate can reach 30 percent improved by more than 50 percent compared with that of the prior art; and the output of the L-cysteine can be improved by more than 20 percent compared with that of the prior art.

The invention discloses a GAD (Glutamic Acid Decarboxylase) gene for increasing stress tolerance of lactic acid bacteria. The GAD gene is characterized in that a gene sequence is shown as SEQ ID NO.1. The gene can be used for increasing the cold stress resistance and acid stress resistance of the lactic acid bacteria. A method comprises the following steps: introducing a recombinant plasmid containing the GAD gene into the lactic acid bacteria and excessively expressing the glutamic acid decarboxylase CsGAD for compounding gamma-aminobutyric acid in lactic acid bacteria, thereby increasing the cold stress resistance and acid stress resistance of the lactic acid bacteria. Thus, the survival rate of the lactococcus lactis under low-temperature and acid environments can be increased, the GAD gene can be used for developing and applying various healthcare foods and an excellent guarantee can be supplied for the industrial production.

The present invention relates to a method of isolating cells from a sample which method comprises binding said cells to a solid support by means of a non-specific ligand immobilised on said solid support, particularly to a method of isolating microorganisms from a sample. Preferred ligands for use in such methods include carbohydrates and derivatives thereof. Also described is a kit for isolating microorganisms from a sample comprising: (a) a solid support having immobilised thereon a ligand which is capable of non-specific binding to microorganisms; (b) means for binding microorganisms to said solid support; optionally (c) means for lysing said cells; and optionally (d) means for binding nucleic acid released from said lysed cells to a solid support.

The invention discloses a 1,3-1,4-beta-glucanase mutant, and belongs to the fields of gene engineering and enzyme engineering. Lysine in the 20th position, 117th position and 165th position of 1,3-1,4-beta-glucanase from Bacillus terquilensis mutates through an overlapping extension PCR method to form serine in order to obtain single mutants K20S, K117S and K165S respectively. The above three mutation sites are integrally mutated to obtain a K20S/K117S/K165S triple mutant enzyme. Four mutant enzymes have higher catalysis activity and better thermal stability. Compared with wild enzymes, the above mutant enzymes are in favor of realizing the industrial application.

The invention discloses an achromobacter xylosoxidans bacterial strain and application thereof for degrading o-aminobenzoic acid. The bacterial strain is (Achromobacter xylosoxidans) N4, the preservation registration number thereof is CGMCC No. 3632. The (Achromobacter xylosoxidans) N4 CGMCC No. 3632 can rapidly and efficiently degrade o-aminobenzoic acid and can be applied to biological treatment on indigo printing and dyeing waste water.

The invention discloses a bdellovibrio bacteriovorus bacterial strain for eliminating listeria monocytogenes and an application thereof. The invention obtains bdellovibrio bacteriovorus BDSM08 by separation, purification and ultraviolet mutation. BDSM08 is in the arc-shaped unicellular form when observed by an electron microscope, the size thereof is 1.8*1.0 mu m, the ends thereof are provided with flagellum and the length of the flagellum is 3.2 mu m. The bdellovibrio bacteriovorus BDSM08 is cultivated with the two layer plating method at the temperature of 28 DEG C for three days to form transparent round negativecolony the diameter of which is 2-3mm and the optimum growing pH value of which is 7.2, the optimum growing temperature of which is 28 DEG C and the optimum salinity of which is0%. The microbial agent prepared by BDSM08 has a strong effect on killing listeria monocytogenes which can be commonly seen in environment. The invention applies the bdellovibrio bacteriovorus BDSM08for the first time to prevent and cure food listerellosis; the invention has a favourable effect on eliminating listeria monocytogenes on food, has characteristics of no toxicity, no side effect on food and the like, and has favourable application prospect.

The invention discloses multipath composite neuraminic acid producing bacillus subtilis and an application thereof, and belongs to the field of genetic engineering. The expression levels of key enzymes, namely UDP-N-acetylglucosamine-2-epimerase, N-acetylglucosamine-6-phosphate isomerase, glucosamine-6-phosphoric acid-N-acetyltransferase, N-acetylglucosamine isomerase and N-acetylneuraminate synthase on genomes in three neuraminic acid synthesis paths are sequentially optimized by 10 promoters with different intensities, so that the protein synthesis pressure of enzyme expression on cells is reduced; and three neuraminic acids are further integrated into the same engineering bacillus subtilis, so that the bacillus subtilis with the increased N-acetylneuraminic acid yield is obtained, the neuraminic acid yield under the shake flask level reaches 10.4 g/L, and a foundation is laid for further increasing the NeuAc yield of the bacillus subtilis.

The invention discloses a compound microbial agent for treating dye waste water as well as preparation and application of the compound microbial agent. The compound microbial agent is formed by complexing acinetobacter haemolyticus, Escherichia coli, bacillus flexus, Yersinia intermedia, luther enterobacter and Klebsiella pneumoniae. Complexing is simple and convenient, the broad spectrum is strong, the difficulty that a single microbial agent can not meet the requirement of water quality variation is overcome, and the cost of treating the dye industrial waste water is reduced.

The invention belongs to the technical field of prevention and treatment of plant diseases and relates to a bacillus amyloliquefaciens strain capable of preventing and treating wheat scab and application thereof. The preservation number of the separated bacillus amyloliquefaciens S76-3 is CCTCC NO:M2014315, and nucleotide of 16S rDNA of the separated bacillus amyloliquefaciens S76-3 is shown in SEQ ID NO:1. The invention discloses separation, identification and broad-spectrum bacteriostasis of the bacillus amyloliquefaciens, spore yield fermentation optimization of the bacillus amyloliquefaciens and field application of the bacillus amyloliquefaciens in prevention and treatment of wheat scab. The liquid inoculant of the bacillus amyloliquefaciens fermentation diluent can be used for effectively inhibiting the wheat scab and harm in the field and reducing toxins in wheat ears. The bacillus amyloliquefaciens strain has strong stress resistance and does less harm to the environment, so that the bacillus amyloliquefaciens strain can be taken as an efficient antibiological inoculant; and the bacillus amyloliquefaciens is broad in antibacterial spectrum and can be used for inhibiting growth of ten plant pathogenic fungi including fusarium graminearum, so that the bacillus amyloliquefaciens strain has a good development and application prospect.

The invention provides a depressive disorder biomarker. The depressive disorder biomarker comprises Clostridium saccharolyticum and Heliobacterium modesticaldum, and also comprises Capnocytophaga, Saccharomonospora viridis, Helicobacter felis, Ruminococcus, Acidaminococcus Rogosa, Acidaminococcus and Acidaminococcus fermentans. The invention further provides a kit for diagnosis of depressive disorder, a composition for preventing or treating depressive disorder and a system for screening a biological sample susceptible to depressive disorder. The system can screen a biological sample susceptible to depressive disorder. The invention further provides a screening method of the biomarker for diagnosis of depressive disorder. The method can screen the biomarker for diagnosis of depressive disorder.

The invention discloses a lactobacillus paracasei as well as a strain domestication method and a culture method thereof. The lactobacillus paracasei is capable of both ensuring that a selenium-containing state refers to organic selenium, and taking physiological functions of probiotics into play. The strain domestication method of the lactobacillus paracasei comprises the following steps: inoculating the lactobacillus paracasei with a lactobacillus paracasei seed culture medium; performing culture; when the OD (Optical Density) value of a culture liquid is up to 1.2-1.6, recording the culturetime as h1; adding a sodium selenite liquid till the content of selenium in the culture liquid is 0.2-1mu g/g; and further performing domestication culture for the time of h1. The culture method of the lactobacillus paracasei comprises the following steps: inoculating a lactobacillus paracasei strain obtained through domestication to a liquid potato culture medium of selenium-enriched potatoes; performing culture; when the OD value of the culture liquid inside the liquid potato culture medium is up to 1.2-1.6, recording the culture time as h2; adding a sodium selenite liquid till the content of selenium in the culture liquid is 0.6-10mu g/g; and further performing domestication culture for the time of h2.

Genetically modified lactic acid bacteria having a reduced or lacking or enhanced diacetyl reductase activity, acetoin reductase activity and/or butanediol dehydrogenase activity are provided. Such bacteria are used in starter cultures in the production of food products including dairy products where it is desired to have a high content of diacetyl and for reducing or completely removing diacetyl in beverages including beers, fruit juices and certain types of wine, where the presence of diacetyl is undesired.

A novel protein having inhibitory activity on the protease activity of hepatocyte growth factor (HGF) activator was purified and isolated, and its molecular weight (ca. 40,000 daltons) and its N-terminal and partial amino acid sequences were determined. A gene coding for the protein was cloned, and the gene DNA was incorporated into a vector, for transforming host cells. Cultivation of the transformant gave the desired protein. The protein can be used as an in vivo or in vitro control factor for HGF or HGF activator. It is also useful as an antigen to be used in producing an antibody to be used as means for kinetic studies of the protein, or as a standard in assay systems therefor.