18 results about "Hydrolysate" patented technology

Disclosed is a process for extracting xylose and xylitol from a xylose mother liquor or a xylose digest, which comprises, using xylose hydrolysate or xylose mother liquid as the raw material, removing glucose through saccharomyces cerevisiae fermentation, then imitating moving bed, using water as eluent, completely separating xylose from foreign matter such as arabinose, thus obtaining component containing rich xylose.

In the biomass hydrolyzing process of preparing reductive monosaccharide, biomass material is hydrolyzed at 100-200 deg.c in the dilute acid catalyst, and the hydrolysate stays in the reactor for 5-40 min. The hydrolysate is divided into three parts based on dydrolysis order and hdyrolysate concentration and the three parts are used as the hydrolysis product and acid solution for the next batch of material to hydrolyze. The partial circulation of the hydrolysate can raise the concentration of monosaccharide in the hydrolysate from 4% to 6% and reduce acid consumption.

The invention discloses an extraction method of ginger essential oil. The method comprises the following steps: preparing ginger powder: slicing fresh ginger, soaking the sliced ginger in a 0.5-0.9wt% starch solution for 10-15min, drying and grinding the sliced ginger, and filtering obtained powder through a sieve of 60-80 meshes; preparing enzymatic hydrolysate: adding a de-ionized liquid 1-ethyl-3-methyl imidazole acetate ([Emim]OAc) to a disodium hydrogen phosphate-citrate buffer solution to obtain an ionic liquid system; adding the ginger powder to the ionic liquid system, controlling a material-to-liquid ratio at (1: 10)g/ml-(1: 20)g/mL, adding cellulase which is 2000-4000U/g in enzyme activity ratio and enzymatically hydrolyzing for 3-5h at 40-50 DEG C; and 3) essential oil extraction: and implementing enzyme deactivation to the system in a water bath after the reaction, ultrasonically extracting for 5-10min, conducting microwave treatment, filtering through gauze, centrifuging, standing and taking the de-ionized liquid on lower layer, spin-steaming, collecting oily matters having intense fragrance and drying the oily matters through anhydrous sodium sulfate to obtain the ginger essential oil.

The invention discloses a method for producing microbial grease by fermenting inulin serving as a raw material. The method comprises the following steps of: hydrolyzing the inulin raw material by using inulase to prepare an inulin hydrolysate, adding yeast extract, peptone, potassium dihydrogen phosphate and MgSO4.7H2O into the inulin hydrolysate to obtain an inulin hydrolysate fermentation medium, inoculating trichosporon cutaneum seed liquid to the fermentation medium, performing fermentation culture, obtaining microbial grease bacteria by centrifuging after culture, drying the microbial grease bacteria, extracting the grease in the cells of the dried microbial grease bacteria by using an organic solvent chloroform-methanol method, and thus obtaining the microbial grease. By the method for producing the microbial grease by using the inulin, the grease raw material source of biodiesel is greatly expanded, the cost of the grease raw material for the biodiesel is remarkably lowered, and meanwhile, the problem of low value utilization of jerusalem artichoke tubers in the prior art is solved.

The invention discloses a non-resistant low-zinc low-copper non-plasma high-grade piglet creep feed and a preparation method thereof. The creep feed comprises the following raw materials: corn, flour,broken rice, puffed soybean powder, milk powder, imported fish meal or fish peptide protein, fermented soybean meal, yeast hydrolysate, soybean protein concentrate, fat powder, acidifying agent, fishsolubles, egg powder, calcium lactate, calcium dihydrogen phosphate, lysine, methionine, threonine, tryptophan, trace element composite package, coated zinc oxide, vitamin composite package, plant extract composite package, plant essential oil, antibacterial peptide and microecological preparation. The product does not use homologous proteins such as plasma protein powder so as to effectively block the occurrence of homologous diseases. The product uses low zinc and low copper so as to reduce environmental pollution and improve the utilization rate of land. The product is free from antibioticso as to reduce drug residue and to ensure human food health. The preparation method provided by the invention is simple, the raw material sources are wide, and the use effect is good.

The invention discloses an efficient preparation method of low-value fish immunomdodulating peptides, relates to the development and utilization of low-value fish bioactive peptides and related anti-inflammatory drug products, and belongs to the technical field of low-value fish processing; the preparation method comprises the steps of preparing nemipterus virgatus myosin by a salt extraction method, fully expanding a high-spiral structural domain of myofibrillar protein by using a high-pressure homogenization method; fine-tuning a protein solution to pH 7.5, adding trypsin, performing enzymatic hydrolysis for 90 minutes, promoting an ultrafiltration membrane to separate a nemipterus virgatus protein enzymatic hydrolysate by using impressed current, and obtain a peptide fragment of 500 to3000 components; finally obtaining a powdery product by a freeze-drying method; the method can improve the degree of hydrolysis of the nemipterus virgatus myosin peptide, and the biological propertiesand yield of the immunomodulating peptides; the anti-inflammatory activity of the immunomodulatory peptides prepared by the method is better; the invention realizes the deep processing and comprehensive utilization of the low-value fish, and has important meaning for improving the added value of the low-value fish.

The invention relates to a preparation method of dendrobium officinale fermentation liquid. The method comprises the following steps of (1) preparing food enzymatic hydrolysate; (2) preparing a dendrobium officinale extraction solution; (3) preparing a dendrobium officinale nutrition solution; (4) performing fermentation; (5) performing emulsification. The dendrobium officinale, moringa oleifera powder and various medical and edible materials are matched in a proper proportion, and are sufficiently mixed; then, lactobacilli are added for fermentation; single strains, i.e., the lactobacilli are used in the fermentation process; a purpose-made culture solution is used as the breeding ground; the fermentation efficiency is more sufficiently realized; the combination of sodium chloride and acetic acid is used as a buffer solution, so that the pH value of the solution is enabled to be maintained in the pre-control range in the fermentation process.

The invention belongs to the field of polymer flooding, and particularly relates to a hydrophobic modifier for improving the blockage removal effect of a polymer waterflooding-blocked well and a polymer flooding method. The hydrophobic modifier is prepared from an organosilane coupling agent, an activating agent, a mutual solvent and water, wherein the activating agent is selected from organic acids with carbon atom numbers of 1-3; the mutual solvent is selected from organic alcohols with carbon atom numbers of 1-3; a mass ratio of the organosilane coupling agent to the activating agent to themutual solvent is (0.5-1.0): (0.01-0.03): (4-10). According to the hydrophobic modifier, the organosilane coupling agent is used for carrying out hydrophobic modification on the surface of sandstonein the presence of the activating agent; in the hydrophobic modification process, hydrolysate of the organosilane coupling agent reacts with a stratum, the surface of sandstone is changed into a hydrophobic state after reaction, wettability is reversed, and then, the adsorbing capacity of a polymer can be remarkably reduced.

The invention relates to a selective separation medium for caprine mycoplasmal pneumonia subspecies and a preparation method of the selective separation medium, and belongs to the technical field of veterinary biology. The selective separation medium is prepared from components as follows: lactoalbumin hydrolysate, yeast extract powder, glucose, an MEM medium, a PPLO (pleuropneumonia-like organism) gravy powder, sodium pyruvate, a 1% phenol red solution, specific growth inhibition serum and deionized water. The preparation method comprises steps as follows: the lactoalbumin hydrolysate, the yeast extract powder, glucose, the MEM medium, the PPLO gravy powder, sodium pyruvate and the 1% phenol red solution are weighed in proportion and dissolved in the deionized water. Before use, healthy horse serum and the specific growth inhibition serum are added. The selective separation medium for the caprine mycoplasmal pneumonia subspecies is used for clinically separating and cultivating the caprine mycoplasmal pneumonia subspecies, increases the growth speed of mycoplasma in a separation process and increases the separation rate of the caprine mycoplasmal pneumonia subspecies, and the separation rate is 80% or higher.

In order to achieve the above object, the present disclosure provides an engineered bacterium strain and the engineered bacterium strain is escherichia coli with a preservation number of CGMCC NO.14817. The present disclosure also provides a method for preparing the engineered bacterium. The method comprises the following steps: recombinant plasmids are transferred to the engineered bacterium hostbacterium, so that the engineered bacterium host bacterium after the transfer can express inserted genes by the recombinant plasmids; the recombinant plasmids comprise a plasmid shown in SEQ ID NO.1,a plasmid shown in SEQ ID NO.2 and a plasmid shown in SEQ ID NO.3. The present disclosure also provides the engineered bacterium prepared by the method, uses of the engineered bacterium strain and engineered bacterium, and a method for preparing farnesene by using cellulose. The disclosed technical scheme significantly improves yield of production of the farnesene by fermentation of cellulose hydrolysates.

The invention provides healthcare medicinal liquor which is prepared by virtue of the following steps: (1), weighing materials according to a formula, wherein the formula comprises the following components in parts by weight: 15-20 parts of radix angelicae, 5-10 parts of saposhnicovia divaricata, 20-30 parts of prepared rehmannia roots, 10-20 parts of seeds of Chinese dodder, and the like; (2), mixing and crushing the following 21 materials such as radix angelicae, saposhnicovia divaricata, prepared rehmannia roots, and seeds of Chinese dodder; (3), adding a traditional Chinese mixture obtained in the step (2) into enzymatic hydrolysate for enzymolysis for 2-4 hours, wherein the mass ratio of the traditional Chinese medicine mixture to the enzymatic hydrolysate is 1:(5-10), and the enzymatic hydrolysate contains 0.5-2wt% of compound enzyme, and the enzymolysis temperature is preferably 36-42 DEG C; (4), drying the zymolytic mixture at 70 DEG C-80 DEG C until the moisture content is 40%-50% (mass); (5), adding borneol into the mixture treated in the step (4), then adding the obtained mixture into liquor and soaking for 30-40 days. The externally-applied healthcare medicinal liquor disclosed by the invention is convenient to use, free from toxic and side effects and capable of effectively alleviating and relieving symptoms of a hyperostosis patient.

The invention provides genetically engineered bacteria synthesizing styrene taking biomass hydrolysate as a raw material and a construction method and application thereof, and relates to the technicalfields of genetic engineering and fermentation engineering. According to the genetically engineered bacteria, L-phenylalanine ammonia-lyase gene, cinnamate decarboxylase gene, 3-deoxy-D-arabinoheptose-7-phosphate synthase gene and bifunctional enzymatic branching acid mutase-prebenzoic acid dehydratase gene are expressed. The construction comprises the steps that the L-phenylalanine ammonia-lyasegene and the cinnamate decarboxylase gene are cloned to be connected to plasmid; the 3-deoxy-D-arabinoheptose-7-phosphate synthase gene and the bifunctional enzyme are cloned to be connected to the plasmid; the plasmid is guided to colibacillus competence to obtain the genetically engineered bacteria. The genetically engineered bacteria can be used for synthesizing the styrene taking the biomasshydrolysate as the raw material and is green and sustainable, the yield of the styrene is greatly higher than the yield of the styrene which is produced by taking the glucose as a carbon source, and the breakthrough which cannot be made all the time is achieved.

The invention provides an on-line separation device for enzymatic hydrolysate. The on-line separation device comprises a separation vessel, an inlet pipe and a vibrator, wherein the separation vesseland an enzymatic tank are respectively connected with two ends of the inlet pipe; the vibrator is internally arranged on one side, close to an entrance, of the separation vessel; the height of the separation vessel is smaller than the width, so that a shallow separating pond is formed; outlet pipes are respectively arranged at the top and the bottom of the separation vessel; each outlet pipe is provided with a photoelectric sensor and a regulating valve. According to the on-line separation device for the enzymatic hydrolysate, disclosed by the invention, an enzymatic mixed solution is fully vibrated and scattered by adopting the vibrator; grease and the enzymatic hydrolysate are layered in the separation vessel by using a shallow pool principle; the opening degrees of the regulating valvesare automatically adjusted according to the difference of the grease and the enzymatic solution to absorbance or reflectance of light, and on-line automatic separation of the grease and the enzymaticsolution is realized, so that standing and layering procedures are reduced and the production time is dramatically shortened. The on-line separation device for the enzymatic hydrolysate, disclosed bythe invention, has the advantages of simple structure, high degree of automation and high accuracy; the grease and the enzymatic hydrolysate can be efficiently and thoroughly separated, the production efficiency is improved and the production cost is reduced.

The invention relates to a preparation method of invert syrup, which comprises the steps of mixing and hydrolyzing a first sugar solution and an acid conversion agent to obtain hydrolysate, and mixing and blending a second sugar solution and the hydrolysate to obtain the invert syrup. The first sugar solution is firstly converted to obtain the converted hydrolysate, and then the converted hydrolysate is blended with the second sugar solution to obtain the invert syrup with any conversion rate, so that the problem that the conversion rate of the invert syrup by an acid method is uncontrollable is solved; furthermore, the first syrup is completely converted, so that continuous conversion of the hydrolysate before and during mixing of the hydrolysate and the second syrup is avoided, the conversion rate of the finally obtained invert syrup is controllable, and the conversion rate can be basically kept consistent with a set value.