23 results about "Tissue culture" patented technology

According to the invention, there is provided seed and plants of the corn variety designated CV252827. The invention thus relates to the plants, seeds and tissue cultures of the variety CV252827, and to methods for producing a corn plant produced by crossing a corn plant of variety CV252827 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV252827 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV252827.

The present invention relates to the abacterial seeding and tissue culture of Paphiopedilum. The Dendrobium is one kind of national first order protected plant and is hard to germinate naturally owing to its incomplete development of embryo. The present invention provides technology of abacterial seeding and tissue culture of Paphiopedilum to obtain great amount of test tube seedling via utilizing artificial nutritious liquid and abacterial seeding, proliferate small seedlings and obtain complete plants via rooting culture. The said seedling growing technology has the advantages of high germination rate, fast seedling growing rate, high seedling quality, etc.

The invention discloses a culture method of an ellcalyplus grandis Hill ex Maid tissue culture seedling, which comprises the following operation steps of taking an ellcalyplus grandis Hill ex Maid tissue culture seedling leaf as an explant, inducing a callus tissue of the ellcalyplus grandis Hill ex Maid leaf, and performing adventitious bud differentiation culture and rootage culture on the callus tissue of the leaf, and is characterized in that the callus tissue of the ellcalyplus grandis Hill ex Maid leaf is induced under the irradiation of red light and blue light, the adventitious bud differentiation culture is performed on the callus tissue of the leaf under the irradiation of the red light or the blue light, adventitious bud growth culture is performed under the irradiation of green light, and then the callus tissue is transferred to be under LED (light-emitting diode) yellow light for the rootage culture. According to the method, light sources are adjusted timely according to difference in requirements of the ellcalyplus grandis Hill ex Maid leaf on the light in different tissue culture stages, and the induction rate, the adventitious bud differentiation rate and the rootage rate of the callus tissue are increased effectively. In addition, the method is low in energy consumption, and can greatly increase the yield of the ellcalyplus grandis Hill ex Maid tissue culture seedling and effectively shorten the seedling emergence time in comparison to the traditional tissue culture method.

The invention relates to a one-time seedling growing method for polygonatum sibiricum. The method includes the first step of culture medium preparation, the second step of explant selection and sterilization, the third step of explant inoculation, the fourth step of culture, and the fifth step of transplanting and hardening. The one-time seedling growing method for polygonatum sibiricum is convenient and fast to implement. Compared with a traditional method that underground rhizomes are selected for tissue culture, tender leaves of polygonatum sibiricum are selected as explants for tissue culture, materials are easy to obtain, and the economic benefit brought by rhizomes of polygonatum sibiricum is not influenced; besides, as the number of leaves of polygonatum sibiricum is large and the propagation coefficient is higher, the production method is convenient and fast to implement, and tissue culture seedlings of polygonatum sibiricum can be cultured within a short time. In addition, the method is easy and convenient to implement, only one step is needed from explants to seedlings, manual work is greatly reduced, and contamination is reduced to the maximum; moreover, by selecting a fine female parent for germplasm purification and rejuvenation of polygonatum sibiricum and maintenance of superior characters, the method has high application value on propagation of fine seedlings of polygonatum sibiricum.

The invention discloses a seedling hardening transplanting method of tissue culture rooting seedlings of Bletilla striata(Thunb.)Reichb.f.. The seedling hardening transplanting method comprises the following steps of 1, conducting seedling hardening in a bottle; 2, conducting cleaning and soaking; 3, conducting seedling hardening outside the bottle, wherein A, seedling hardening at the first proper temperature is conducted, B, seedling hardening at the low temperature is conducted, C, seedling hardening at the second proper temperature is conducted, and D, seedling hardening at the normal temperature is conducted. By means of the seedling hardening transplanting method of the tissue culture rooting seedlings of Bletilla striata(Thunb.)Reichb.f., the infection of tissue culture seedlings byinfectious microbes is greatly lowered, the survival rate is increased, cultivation of healthy seedlings is facilitated, the method is significant in increasing the field planting survival rate and prompting subsequent growth, and thus a basis is laid for improving the product quality and increasing the product yield by a large margin. Grading seedling hardening is conducted, the method has a more scientific adaptation period, the transplanting survival rate is greatly increased, and the seedling recovering time is shortened; the seedling hardening survival rate can reach 99% or above.

The invention discloses a genetic transformation method by the utilization of cotton meristematic tissue in the plant gene engineering field. According to the method, without the step of cotton tissue culturing, activated Agrobacterium containing target gene plasmid vector is directly dipped on a growing point removed cotton plant to induce adventitious bud to grow until the adventitious bud blossoms and grows bolls, and transgenic cells are screened by the use of antibiotics. By the utilization of the method for genetic transformation of cotton, differentiation rate is high, the screening method is simple, the cost for cotton genetic transformation is saved, and the time for cotton genetic transformation is shortened.

The invention relates to a proliferation culture medium formula in blueberry tissue culture, which consists of the components with different concentrations as follows: macroelements, microelements, iron salts, organic compositions and the like. By using the culture medium, the proliferation efficiency can be as high as more than 15 times; and the proliferation culture time is as short as only 25-30 days, and proliferation seedlings can reach 3-4cm in height, thus fully satisfying the need for rooting culture. Simultaneously, the culture medium consists of the compositions which have common sources without compounds with special forms, and has easy preparation.

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH569965. The invention thus relates to the plants, seeds and tissue cultures of the variety CH569965, and to methods for producing a corn plant produced by crossing a corn plant of variety CH569965 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH569965.

The invention provides a method for large-scale planting of high-yield siraitia grosvenorii. The method comprises the following steps of land selection and preparation, seedling hardening, planting, water and fertilizer management, pollination, field management and the like. According to the method, by combining the trellis growing time, the flowering time and the fruit maturation time in all thetreatment steps, the time from planting to trellis growing, flowering and fruiting is gradually shortened; meanwhile, the dead seedling rate of seedlings starts to be reduced, the fruit cracking rateis reduced, and the average plant yield of the siraitia grosvenorii is increased. Meanwhile, by carrying out high-yield cultivation technology research on the siraitia grosvenorii tissue culture seedlings, the appropriate planting time, transplanting mode, pollination time and the effective active time of pollen are determined, which lays a foundation for high-yield cultivation and large-scale planting of siraitia grosvenorii.

According to the invention, there is provided seed and plants of the corn variety designated CV911339. The invention thus relates to the plants, seeds and tissue cultures of the variety CV911339, and to methods for producing a corn plant produced by crossing a corn plant of variety CV911339 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV911339 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV911339.

The invention discloses a lithocarpus carolinae tissue culture explant obtaining method which comprises the working procedures of seed selection and sowing, bud seedling transplanting and management, mother plant cultivation and management and explant obtaining. Mother plants subjected to selection of high-quality seeds and high-quality robust seedlings are cultivated, and after being cut from the mother plants, proper buds are disinfected and inoculated. According to the lithocarpus carolinae tissue culture explant obtaining method, mother plant management and bud collection are very convenient; an obtained explant is high in quality, low in pollution rate and death rate, high in adventive bud germination rate, tidy in bud growth and high in operability; the demand for building a lithocarpus carolinae tissue culture rapid propagation system is met; the lithocarpus carolinae tissue culture explant obtaining method has relatively good economic, social and ecological benefits.

The invention relates to the novel cotton variety designated 03Y062. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 03Y062. Also provided by the invention are tissue cultures of the cotton variety 03Y062 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 03Y062 with itself or another cotton variety and plants produced by such methods.

The invention provides a castor-oil plant tissue culture plants concentrated propagation method, which is used for tissue culture plants propagation of different lines. Castor-oil plants of different lines are cultured in a concentrated manner, and the castor-oil plants of each line in a squaring stage are placed in an isolation chamber so that the castor-oil plants of different lines will not affect each other. Concentrated seedling cultivation is carried out, the plants need not to be planted in separated different areas, the planting efficiency is improved, and observation and recording in a phonological period is convenient. Isolation is conducted in the squaring stage to ensure that cross infection among the plants will not happen, therefore, the survival rate and the reproduction rate are increased.

The invention provides a cow interdigital skin explant model construction method, and belongs to the technical field of skin explant models. The model construction method comprises the following steps: immersing the interdigital skin of the dairy cow into a transportation culture medium for transportation, cleaning the interdigital skin of the dairy cow by using a washing culture medium after transportation is completed, and culturing the cleaned interdigital skin of the dairy cow in a cell chamber containing a tissue culture medium to complete model construction; and finally, carrying out feasibility evaluation on the cow interdigital skin explant model. The constructed 3D dairy cow interdigital skin model conforms to experimental animal 3R principles of reduction, substitution, optimization and the like, meanwhile, the constructed model can maintain good tissue structure integrity, the dairy cow interdigital skin in-vivo environment is fully simulated, and the method has great significance in deep research of dairy cow interdigital skin pathogenic microorganism invasion action mechanism.

The invention provides a rosaceae cerasus plant ex-vitro rooting method, and relates to the technical field of tissue culture rooting. According to the rosaceae cerasus plant ex-vitro rooting method, a rooting environment in a test-tube plantlet bottle is simulated, so that tissue culture plantlets root in a relatively closed matrix with a nutrient solution, then the nutrient solution and a plastic film are gradually removed, and a matrix culture stage is directly carried out. According to the rosaceae cerasus plant ex-vitro rooting method, the rooting process and the transplanting process of tissue culture seedlings in the bottle are combined, so that the tissue culture seedlings directly root outside the bottle and in a transplanting medium, the production link is simplified, the production cost is reduced, and the emergence rate is increased. According to the rosaceae cerasus plant ex-vitro rooting method, ex-vitro rooting is carried out on the cerasus humilis tissue culture seedling, and the rooting rate can reach 80.6% at most.

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH157613. The invention thus relates to the plants, seeds and tissue cultures of the variety CH157613, and to methods for producing a corn plant produced by crossing a corn plant of variety CH157613 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH157613.

The invention relates to a tissue culture method of syringa microphylla diels and a method for rapidly obtaining large syringa microphylla diels seedlings. The tissue culture method comprises the following steps of (1) explant selection and disinfection: cutting the top end part of an annual tender branch, cutting the top end part into a stem segment with a terminal bud or 1-2 axillary buds as anexplant, and carrying out disinfection treatment; (2) starting culture: putting the explant into a starting culture medium, and enabling the terminal bud or the axillary bud to be just in contact withthe culture medium until the terminal bud or the axillary bud germinates, wherein the starting culture medium takes an MS culture medium as a basic culture medium, 30g/L of saccharose, 6g/L of agar,1mg/L of hormone 6-BA and 0.2 mg/L of hormone NAA are added at the same time, and the pH is adjusted to 5.8-6.2; (3) carrying out subculture; (4) performing rooting culture; and (5) performing transplanting to obtain tissue culture seedlings. According to the method, the survival rate of the syringa microphylla diels explants in the starting culture process is high, calluses are not prone to appearing, and the induction rate of axillary buds or terminal buds is high.