Tissue culture method of syringa microphylla diels and method for rapidly obtaining large syringa microphylla diels seedlings

[0058] Step 1: At the end of April, cut off 7-8cm of the top part of the new young shoots of healthy and pest-free plants. Remove all the leaves from the cut stems and rinse them under running water for 30 minutes. Alcohol will cause a certain degree of damage to the explants. The selected stems are too tender, skip alcohol sterilization, directly soak in sodium hypochlorite solution with 1% available chlorine for 20 minutes, rinse 3 times with sterile water, and dry the stems. Cut to 1-1.5cm and ensure that each stem segment has terminal buds or 1-2 axillary buds, and the explant survival rate is 80%;

[0059] Step 2: Start the cultivation, select a stem segment with a terminal bud or an axillary bud, the length is about 1-1.5cm. When inoculating, the buds should just touch the medium, so that the buds can effectively absorb nutrients and make the seedlings strong in the later subculture process. , with many differentiations. Using MS as the basal medium, add 30g / L sucrose,

[0066] Step 1: Cut off the top part of 7-8cm from the top part of the new young shoots of healthy and pest-free plants in early May. Remove all the leaves from the cut stems and rinse them under running water for 30 minutes. Alcohol will cause a certain degree of damage to the explants. The selected stems are too tender, skip alcohol sterilization, directly soak in sodium hypochlorite solution with 1% available chlorine for 20 minutes, rinse 5 times with sterile water, and dry the stems. Cut to 1-1.5cm and ensure that each stem segment has terminal buds or 1-2 axillary buds, and the explant survival rate is 82%;

[0067] Step 2: Initiate culture, select a stem section with terminal buds or two axillary buds, the length is about 1-1.5cm. When inoculating, the buds should be just in contact with the initiation medium, so that the buds can effectively absorb nutrients, so that the seedlings in the later subculture process Strong, well differentiated. Start the medium with MS as th

[0074] Step 1: Cut off the top part of 7-8cm from the top part of the new young shoots of healthy and pest-free plants in early May. Remove all the leaves from the cut stems and rinse them under running water for 30 minutes. Alcohol will cause a certain degree of damage to the explants. The selected stems are too tender, skip alcohol sterilization, directly soak in sodium hypochlorite solution with 1% available chlorine for 20 minutes, rinse 5 times with sterile water, and dry the stems. Cut to 1-1.5cm and ensure that each stem segment has terminal buds or 1-2 axillary buds, and the explant survival rate is 82%;

[0075] Step 2: Initiate culture, select a stem section with terminal buds or two axillary buds, the length is about 1-1.5cm. When inoculating, the buds should be just in contact with the initiation medium, so that the buds can effectively absorb nutrients, so that the seedlings in the later subculture process Strong, well differentiated. Start the medium with MS as th