40 results about "Bud" patented technology

The invention discloses a rapid cultivation method for Chinese rose landscape modelling. The method includes the steps of planting grafted seedlings or cutting seedlings in a cultivation container, and managing water and fertilizers in an initial planting stage; binding and sizing a modelling support on the top of the cultivation container after rust prevention of the modelling support; binding the main branches of limbing roses from bottom to top along the base of the modelling support, and according to 30-70 degrees of a lateral angle on the main branches, binding the main branches at the outer side of the modelling support; binding first-grade branches uniformly on the modelling support by a lateral angle of 30-70 degrees, and binding second-grade branches in the same 30-70 degree lateral mode; when the main braches, the first-grade branches and the second-grade branches of the limbing roses grow and are bound until the modelling support cannot be seen, obtaining the modelled Chinese rose landscape modelling. The method can promote bud germination, growth and flowering, not only the leaf coverage rate is high, the landscape modelling is complete, the seedling-planted rose modelling culture period is short, and the modelling can be formed in the current year, but also the formed modelling can be moved.

The invention discloses an in-vitro rapid propagation method of rare and endangered plant ammopiptanthus mongolicus, belongs to the biological field and aims to solve the problems of severe browning and differentiation difficulty in callus induction in existing methods. The in-vitro rapid propagation method of the ammopiptanthus mongolicus comprises steps of obtaining of ammopiptanthus mongolicus explants, induction culture of cluster buds, extension culture of the cluster buds, rooting culture and expansion propagation. Compared with existing in-vitro culture methods of the ammopiptanthus mongolicus, the method has the advantages that cotyledonary nodes are directly subjected to induction of cluster buds and rooting is performed, callus cannot be produced in the process, thus, the step of callus induction is omitted, the cotyledonary nodes are directly cultured into adventitious buds, the in-vitro culture time is greatly shortened, and the whole process from seed germination, obtaining of cotyledonary nodes, induction and extension of the cluster buds to rooting only takes 79 d.

The invention discloses a culture method for a brassica oleracea L. var. acephala microspore regeneration plant. The method comprises the following steps: taking inflorescences of brassica oleracea L. var. acephala and rape, directly taking or taking after induction alabastrum of the inflorescences, adding the alabastrum into NLN-13 induced medium after disinfection to form fluid suspension, carrying out filtering, and centrifuging the filtrate to obtain a precipitate; adding NLN-13 induced medium and active carbon mixed liquor in order so as to obtain a microspore fluid suspension; carrying out a heat shock treatment, followed by culturing so as to obtain embryoids in cotyledon stage; inoculating the embryoids to embryoid differential medium until the embryoids are differentiated and regenerates into buds; cutting the regenerated buds to a rooting medium for rooting culture, and hardening and transplanting seedlings to obtain regenerated plants; taking young leaves of the regenerated plants for the detection of ploidy of corresponding regenerated plants and determining regenerated seedlings of rape and brassica oleracea L. var. acephala in the regenerated plants. According to the method provided in the invention, rape which is easy to generate embryos and brassica oleracea L. var. acephala which is difficult to generate embryos are mixedly cultured; the material which is easy to generate embryos is used to spur the material which is difficult to generate embryos; therefore the ratio of embryos of brassica oleracea L. var. acephala is improved.

The invention discloses a fresh flower preservative and a preparation method thereof. The fresh flower preservative is prepared from the following components in parts by weight: 70-80 parts of ethanol, 40-50 parts of ethyl pyruvate, 10-20 parts of citric acid, 8-15 parts of sodium citrate, 3-8 parts of sodium thiosulfate, 1-5 parts of stearic acid, 1-5 parts of salicylic acid, 0.5-2 parts of potassium sulphate, 1-5 parts of monopotassium phosphate, 5-10 parts of thiourea and 30-40 parts of water. The preparation method comprises the following steps: mixing ethanol and water, heating to 30-35 DEG C, adding with ethyl pyruvate, stirring for 10-20 min, then adding the other components, stirring for mixing uniformly, cooling to room temperature, and sealing for storage. With the fresh flower preservative, the flowering phase can be prolonged by 15-20 days on average, and meanwhile, fresh flowers with damaged branches, buds and leaves can bloom into bright flowers, so that the utilization ratio of the fresh flowers is greatly improved.

The invention discloses a dioscorea opposita mechanical ridging directional cultivating method capable of preventing tuber deformation. The method comprises the following steps: step 1, selecting a land and preparing: step 2, ridging wide and narrow rows, namely, forming two rows of single large ridges through ridging once by a large tractor; step 3, deeply burying a guide pipe or a spacer, namely, digging a 10cm-wide, 15-20cm-deep and 100cm-long small furrow from the ridge top along the ridge slope according to a plant distance of 20-30cm, padding the semi-circular guide pipe or the plastic spacer in the furrow, covering one layer of fine soil along the furrow to press the guide pipe or the plastic spacer, and then scattering a fertilizer and covering soil; step 4, sowing bulbils or tubers in soil which is about 5cm above the buried guide pipe or plastic spacer, and thickly covering 20-30cm of fine soil; step 5, picking buds, namely, leaving one bud on each plant, and picking off the rest buds in time; step 6, performing field management and harvesting. The labor intensity of dioscorea opposita ridging and harvesting can be obviously reduced, and the tuber deformation rate obtained by cultivation is obviously reduced.

The invention discloses a method for improving the yield of muskmelon hybrids by using hormone treatment. The method comprises the following steps of: (1), collecting male parent flowers, namely, in the afternoon of the day before fertilization, intensively collecting male flower buds blossoming in the next day from male parent plants of muskmelon, placing in a glass bottle, keeping in a dark place; (2), selecting and pollinating female parent flower, namely, in the next day after collecting of male flowers, selecting buds blossoming in the next day from the female parent plants of muskmelon, removing anther, smearing anther of male flowers collected in the step (1) on female flower stigmas, isolating by covering with paper caps; and (3), carrying out hormone treatment on fertilized fruits, namely, 24-72h after the pollination process completed in the step (2), removing the paper caps at a temperature of 18-30 DEG C and dipping the flowers in a 2.5ppm-5ppm forchlorfenuron water solution. By adoption of the method provided by the invention, the fruit setting rate and the single-melon seed setting rate are increased, and the effects of saving the labor for pollination and increasing the yield are achieved.

The invention relates to the technical field of biomedicine, in particular to a liver bud stem cell, a preparation method and the use thereof. The stem cell biology obtains the rapid development in the recent years. The isolation, the culture and the in-depth research of liver stem/progenitor cells have very important values for the occurrence mechanism of liver development, liver tumor and liver regeneration and the prevention and the treatment of various liver diseases. The currently and successfully cultured liver stem/progenitor cell systems are very limited. The invention is the multipotential stem cell which is isolated from a human embryonic liver bud tissue with the pregnancy of 4.5 to 6 weeks, thereby having significance for knowing the liver development, the regeneration and other related medical problems, providing an ideal cell model for the research of the liver stem cell biology and possibly providing an ideal cell source for the research of the cell therapy of liver diseases caused by various factors, the drug screening, the tissue engineering and other fields.

The invention discloses a culture method of an ellcalyplus grandis Hill ex Maid tissue culture seedling, which comprises the following operation steps of taking an ellcalyplus grandis Hill ex Maid tissue culture seedling leaf as an explant, inducing a callus tissue of the ellcalyplus grandis Hill ex Maid leaf, and performing adventitious bud differentiation culture and rootage culture on the callus tissue of the leaf, and is characterized in that the callus tissue of the ellcalyplus grandis Hill ex Maid leaf is induced under the irradiation of red light and blue light, the adventitious bud differentiation culture is performed on the callus tissue of the leaf under the irradiation of the red light or the blue light, adventitious bud growth culture is performed under the irradiation of green light, and then the callus tissue is transferred to be under LED (light-emitting diode) yellow light for the rootage culture. According to the method, light sources are adjusted timely according to difference in requirements of the ellcalyplus grandis Hill ex Maid leaf on the light in different tissue culture stages, and the induction rate, the adventitious bud differentiation rate and the rootage rate of the callus tissue are increased effectively. In addition, the method is low in energy consumption, and can greatly increase the yield of the ellcalyplus grandis Hill ex Maid tissue culture seedling and effectively shorten the seedling emergence time in comparison to the traditional tissue culture method.

The present invention discloses a grain bud with the high activity of saponin or glycoside glycosidase and an application thereof. The grain bud with the high activity of the saponin or the glycoside glycosidase is prepared by a method as follows: gibberellin and ginseng saponin or windflower saponin or yam saponin or astragalus saponin or icariine or isoflavone glycoside or rutin glycoside are added during the process of preparing the grain bud including barley, broomcorn, corn and wheat bud, to ensure that the grain bud germinates and is dried to respectively obtain the corresponding grain bud with the high activity of the saponin or the glycoside glycosidase. The present invention has simple making technology and low cost, has high hydrolyzing capacity to the main active components of the saponin and glycoside glycosyl in Chinese herbal medicines and can be used for hydrolyzing the ginseng saponin or windflower saponin or yam saponin or astragalus saponin or icariine or isoflavone glycoside or rutin glycoside to prepare the corresponding secondary low-glycosyl saponin or glycoside with high activity.

The invention discloses a traditional Chinese medicine for treating anemia. The traditional Chinese medicine comprises the following components in part by weight: 50 to 100 parts of barbary wolfberry fruit, 50 to 100 parts of Chinese date, 50 to 100 parts of virgate wormwood herb, 50 to 100 parts of honeysuckles, 50 to 100 parts of donkey-hide glue, 50 to 100 parts of black sesame, 50 to 100 parts of longan, 40 to 80 parts of Chinese lobelia herb, 40 to 80 parts of astragals, 40 to 80 parts of scrophularia root, 40 to 80 parts of hairyvein agrimonia herb and bud, 40 to 80 parts of isatis indigotica fort, 40 to 80 parts of rehmanniae praeparatum, 40 to 80 parts of dwarf lilyturf tuber, 40 to 80 parts of weeping forsythiae capsule, 45 to 85 parts of houttuynia, 45 to 85 parts of gardenia, 5 to 85 parts of Indian buead,45 to 85 parts of golden thread and proper amount of honey and dextrine. The components of the traditional Chinese medicine for treating anemia are prepared into the medicament by processes of crushing, extracting, mixing, pelleting and the like. The formula of the traditional Chinese medicine has very good treatment effect on people with anemia and qi-blood deficiency and also has relatively good adjuvant treatment effect on patients with leukemia.

The invention relates to a cedrela sinensis budding and propagating method. The method comprises the followings: selecting annual tree seedlings of cedrela sinensis, which grow strongly and have a ground diameter of 1.0 to 1.2 cm, as rootstocks, and adopting annual branches which are robust, in full buds and free of plant diseases and insect pests from a cedrela sinensis seed tree for budding, so as to realize cedrela sinensis micropropagation. The budding specifically comprises the followings: selecting strong buds in the middles of the branches for scion, transversely cutting in places which is above the buds and is 0.5 cm far away from the buds, and then transversely cutting in places which are below the buds and are 0.5cm far away from the buds, wherein each bud piece requires a part of wood part to be carried, the thickness is 2 to 3 mm, and the lengths of the cutting surfaces of the bud pieces are 1.0 to 1.5 cm; selecting northeast surfaces of the rootstocks, cutting off skin blocks in positions 5 cm far away from the ground, (the rootstock cutting method is the same as the scion), then taking down the cut skin blocks, quickly adhering the bud pieces onto the cutting surfaces, enabling the lower ends of the bud pieces to be aligned with the lower cuts of cut surfaces, and then using ropes for tightening. The method provided by the invention has the characteristics of simple operation and high ratio of grafting, the cultivated seedling is high, the diameter growth is fast, and a good foundation is established for storing and using cedrela sinensis fine breeds in future.

The invention discloses a simple grafting tool special for pitaya. The tool comprises a cutting tool of which a body is of a hollow structure, one end of the body structure forms a holding end, the other end of the body structure is provided with a blade, and the blade is distributed at a hollow port along the body structure to form a cutting end. The invention further discloses a rapid grafting method utilizing the simple grafting tool special for the pitaya. The method comprises the following steps that proper time is selected for grafting work, a robust rootstock plant and a scion bud plantexcellent in variety are selected, a scion bud with an areole is cut out by means of the dedicated grafting tool, a corresponding cutting opening is cut out on rootstock, the scion bud with the areole is placed in the cutting opening of the rootstock, the areole with the exposed scion bud is glued and fixed, an original areole on the rootstock is cut out, the rootstock is sleeved with a plastic bag, moisturizing is conducted till the grafted portion heals completely, and then the bag is removed. Accordingly, pitaya grafting and seedling raising and pitaya high grafting for changing breed become easier, operation is easy, the efficiency is high, the number of ears for single bud grafting is small, and popularization is facilitated.

The invention discloses a genetic transformation method by the utilization of cotton meristematic tissue in the plant gene engineering field. According to the method, without the step of cotton tissue culturing, activated Agrobacterium containing target gene plasmid vector is directly dipped on a growing point removed cotton plant to induce adventitious bud to grow until the adventitious bud blossoms and grows bolls, and transgenic cells are screened by the use of antibiotics. By the utilization of the method for genetic transformation of cotton, differentiation rate is high, the screening method is simple, the cost for cotton genetic transformation is saved, and the time for cotton genetic transformation is shortened.

The invention discloses a sludge sampler which comprises a handle, a sampling rod and a sampling cylinder, wherein one end of the sampling rod is connected with the handle, and the other end of the sampling rod is connected with the sampling cylinder; a containing cavity of which the bottom is opened is formed in the sampling cylinder; a water pump and a control panel are arranged on the samplingcylinder; the water pump is electrically connected with the control panel; the control panel is used for controlling the operation of the water pump; the water inlet of the water pump is communicatedwith the bottom of the sampling cylinder; and the water outlet of the water pump is formed in the outer surface of the sampling cylinder. According to the sludge sampler disclosed by the invention, the aim of removing sediment upper water entering the sampling cylinder can be achieved, and the condition that the sediment upper water enters bottom bud pores on the lower layer so as to influence subsequent measurement results in the deposit process is avoided.

The invention relates to a top dressing flavor for improving influences of Chinese herbal medicine extracts on cigarette taste. The top dressing flavor is prepared by mixing the following raw materials by weight: 70.6-74.5% of 95% alcohol, 0.3-0.5% of michelia alba leaf oil, 0.2-0.5% of clove bud oil, 0.1-0.2% of lavender oil, 0.2-0.5% of geranium oil, 0.8-1.0% of eugenol, 0.3-0.5% of aniseed alcohol, 0.5-0.8% of dihydroactinidiolide, 0.2-0.5% of ethyl acetoacetate, 0.1-0.2% of ethyl levulinate, 10% of propylene glycol, 2.5-5% of essence tincture, and the balance of water. When the top dressing flavor of the invention is added into tobaccos, the smoke is fine with sweet feel; the mouth is clean; the taste is improved; and the cigarette quality is improved.

The invention provides a method for remediating polychlorinated biphenyl polluted soil through the combination of chemical elution and plants, and belongs to the field of polluted soil remediation. The method comprises the following steps that the polluted soil to be remediated is collected, ground, crushed and screened through a 60-mesh sieve, the water content of the screened soil is adjusted to be 40%-60% of the field water capacity of the soil, and the soil is kept for standby application; medicago sativa seeds with white buds being exposed through bud forcing treatment are evenly sown in the standby soil and cultivated, medicago sativa plants are thinned out after growing for two weeks, and the cultivation period of the medicago sativa plants is 90 days; and the process that eluant is sprayed into the soil is started from thinning-out, the eluant is sprayed once every 5-8 days, and thus polychlorinated biphenyls in the soil are removed. Compared with the prior art, the method is advantageous in that operation is easy, the treatment effect is good, and in-situ remediation can be achieved; the influence on the original environment is small in the remediation process, and the method can be used for a wide range of types of soil; when the method is used for practical environmental remediation, the secondary pollution risk is greatly reduced, and follow-up soil cyclic utilization is facilitated.

The invention mainly relates to the technical field of planting, and discloses a planting method for lolium multiflorum dedicated to cows. The planting method comprises the following steps: performing seed treatment; adding fertilizer to bud seedlings; tillering and applying additional fertilizer; regularly mowing; the method is simple and convenient to operate; the obtained lolium multiflorum is rich in nutrients; the protein content reaches 27.84 percent; the fresh weight yield during an elongation stage reaches 90.7 t/hm<2>; the yield and the quality of the lolium multiflorum are improved; the lolium multiflorum seeds are soaked in a low-temperature acetate solution so as to accelerate the softening of seed skin and promote the immersion of the nutrients required for seed germination; the low-temperature acetate solution also contain honey and urea so as to improve germ activity, promote seed germination and improve the seed germination rate; the seed germination rate reaches 92.7 percent; during bud seedling period, a multi-bacterium liquid is sprayed at dusk, water evaporation is slowed, full contact between the multi-bacterium liquid and the bud seedlings is promoted, the absorption and utilization rate of the multi-bacterium liquid is improved, the bud seedling growth is accelerated, and the stress resistance of the bud seedlings is enhanced.

The invention discloses a preparation method of Allium tenuissimum L. essential oil. The method comprises the following steps: (1) picking Allium tenuissimum L. flower buds, and adding the Allium tenuissimum L. flower buds and a 0.5 to 2.5 percent by mass table salt solution into a distillation kettle together; (2) heating a mixture in the distillation kettle to a boiling state with steam, and performing distillation; (3) when the temperature in the kettle is 50 to 70 DEG C, introducing cooling water, controlling the temperature of liquid flowing out of a condenser at 20 to 25 DEG C through the cooling water after boiling, heating the effluent liquid to 25 to 30 DEG C 0.5 hour later, and introducing the heated effluent liquid into an oil-water separator; (4) returning distillate separated by the oil-water separator into the distillation kettle by reflowing, wherein reflowing lasts for 3 to 4 hours; (5) after oil and water are delaminated fully, opening the oil-water separator, and collecting an oil layer to obtain the Allium tenuissimum L. essential oil. The plant essential oil prepared by the method is closer to the natural fragrance of the Allium tenuissimum L. flower buds, and the quality, yield and purity of the Allium tenuissimum L. essential oil are ensured.

The invention discloses a seedling-raising substrate and a preparation method thereof, and relates to the field of plant seedling raising. The seedling-raising substrate is prepared from, by mass, sandy soil, magnesium sulfate, rotten cow dung, a water-absorbing water-retaining agent, carburizing rice husk, trachycarpus fortunei bark fibers, calcium magnesium phosphate fertilizer, nitrogen-phosphorus-potassium compound fertilizer, ammonium sulfate, monoammonium phosphate, boric acid, polyaspartic acid, glucosamine oligosaccharides, a plant disease prevention agent and activation resistant bacteria. According to the technical scheme, the substrate has the functions of sufficient fertilizer efficiency, nutrition slow release, disease resistance and stress tolerance, comprehensive nutrition, high pertinence and the like, and various indexes reach the industrial standard. By means of batched production and application in multiple seedling-raising factories, the seedling-raising substrate can increase the germination rate of seeds and seedling emergence rate, seedlings are regular, robust growth is achieved, the seedlings are healthy and strong, rampant growth, root rot, dwarfing and rigidifying do not exist, flower bud differentiation of the seedlings is normal, and the property, yield and quality of the crop variety are guaranteed.

The invention discloses a Chinese medicine decoction for treating hiccup and a preparation method of the Chinese medicine decoction. The Chinese medicine decoction comprises the following raw materials in part by weight: 3 to 4 parts of lilac, 5 to 7 parts of persimmon calyx, 10 to 12 parts of ruddle, 3 to 4 parts of osmanthus fragrans lour, 3 to 4 parts of rose, 6 to 9 parts of green tangerine peel, 6 to 9 parts of Chinese hawthorn leaf, 4 to 5 parts of jasminum amplexicaule buch, 10 to 12 parts of mallotus root, 6 to 9 parts of chestnut shell, 10 to 12 parts of hawthorn, 4 to 6 parts of fleshfingered citron flower, 3.5 to 4.5 parts of sunflower flower disc, 4 to 6 parts of japanese metaplexis pericarp and 2 to 4 parts of lilac daphne flower bud. The Chinese herbal medicine has the effects of calming adverse-rising energy, descending qi, calming the liver, suppressing yang, nourishing yin, clearing away heart-fire, relieving restlessness, soothing the nerves, resolving dampness, stimulating appetite, warming spleen and stopping diarrhea, and has the characteristics of quick effect in hiccup treating, no stimulation, no toxic and side effects, convenience in taking and strong adaptability.

The invention provides a Chinese rose soilless culture nutrition solution. The nutrition solution comprises a mother liquor A, a mother liquor B and a mother liquor C, and the mother liquor A comprises 200-350mg/L of KH2PO4, 110-170mg/L of NH4H2PO4, 260-390mg/L of MgSO4.7H2O and 255-410mg/L of CaCl2.2H2O; the mother liquor B comprises 5-9mg/L of ZnSO4.7H2O, 0.6-0.9mg/L of CuSO4.5H2O, 5.5-8.7mg/L of H3BO3, 0.02-0.04mg/L of K2MoO4, 5-15mg/L of (NH4)2MoO4, 0.12-0.18mg/L of Na2MoO4.2H2O and 1-3.5mg/L of MnSO4.4H2O; and the mother liquor C comprises 70-85mg/L of calcium superphosphate, 68-74mg/L of Na2Fe-EDTA and 20-50mg/L of K2SO4, the mother liquor A, the mother liquor B and the mother liquor C are blended according to a volume ratio of 1:1:1, the obtained liquor mixture is diluted 6 times, and the diluted liquor mixture can be used. The nutrition solution can effectively promote growth of Chinese rose, and can substantially shorten the bud stage of the Chinese rose.

The invention discloses a radish generation-adding seeding method. The method mainly comprises the step that a simple transplanting method of germinated radish seeds is adopted, wherein a 1.2-meter flat bed is selected, water is poured into the bed surface thoroughly, after the water percolates down, blunt-ended forceps are adopted for clamping root tails of buds and pressing them into mud, and cotyledons and the portion 2-3 mm away below the cotyledons are reserved and exposed above the ground. Operation is easy, and the survival rate is high. Due to the fact that links such as ditching or digging, bottom water irrigation and earthing are reduced, the efficiency is doubled compared with that of a conventional method.

The invention discloses an ailanthus altissima efficient in-vitro culture plant regeneration method. The method comprises the procedures of explant selection, explant treatment, initial bud induction culture, multiplication culture, strong bud culture and rooting culture, and comprises the following specific steps: collecting a semi-lignified robustly-growing annual ailanthus altissima shoot to serve as an explant; trimming the explant, sterilizing and disinfecting the trimmed explant, and inoculating the sterilized and disinfected explant into an initial bud induction culture medium to obtain an initial bud; inoculating the initial bud into a multiplication culture medium, and performing multiplication culture for 3 to 4 periods to form cluster buds; inoculating the cluster buds into a bud strengthening culture medium for culturing; lastly, inserting single buds which are more than or equal to 2cm in heights into the rooting culture medium to induce rooting. By adopting the method, the ailanthus altissima seedling period is shortened, the seedling material is saved, the problems of need of a large quantity of seedling materials, long period and the like in the conventional seedling method are solved, the production cost is lowered greatly, the production efficiency is increased, and good economical benefit, social benefit and ecological benefit are achieved.