Method for rapidly restoring lily distant hybrid fertility
一种远缘杂种、快速恢复的技术,应用在园艺方法、植物学设备和方法、植物基因改良等方向,能够解决进口种球手续繁杂、病虫害、质量不好等问题
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[0015] 1) Cultivation of distant lily: choose a healthy and free species of distant lily with a bulb circumference greater than 12 cm, and carry out lily cultivation and management according to conventional methods;
[0016] 2) Treatment of lily buds: Select bulbs of distant lilies that can bloom, and cultivate them by conventional methods. When the buds appear, use cotton balls dipped in 4-10μM concentration of amisulfame solution to cover the buds on the tip of the stem, and use 4-10μM The flower buds of the distant hybrid lily were treated with amisulfameline for 2-3 days, and then washed with water for 5 times.
[0017] 3) Fertility recovery identification: The sign of fertility recovery of distant hybrids of lilies is that their pollen is fertile, and untreated pollen is sterile, and pollen germination cannot be seen under a microscope. The fertile pollen grains obtained from the treatment are gigantic, and the pollen can be seen to germinate and form pollen tubes under a...
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