One-time seedling growing method for polygonatum sibiricum

A technology of Polygonatum chinensis and seedling refining, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effect of high application value, easy technology to master, and reduction of manual work.

Active Publication Date: 2016-11-23
恩施州源惠科技开发有限公司
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented process allows creating new plants called polygons that have better properties than existing ones during their first year's growing season or after they were planted outdoors over winter months without causing environmental damage due to excessive use of soil resources. It also makes them easier to grow into consistently good quality seeds quickly while reducing labor costs associated therewith.

Problems solved by technology

The technical problem addressed by this patented study is how to efficiently produce large amounts (upwards) of polygonal tubers with improved reproductive capacity while also reducing costs associated with conventional methods like cutting off their stalk or growing new ones.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • One-time seedling growing method for polygonatum sibiricum
  • One-time seedling growing method for polygonatum sibiricum
  • One-time seedling growing method for polygonatum sibiricum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: a kind of Rhizoma Polygonatum one-time seedling-growing method of the present invention is characterized in that containing following steps: a. the configuration of medium: add 6-BA and 2,4-D in MS basic medium, and make its concentration Reach 2mg / L and 0.5mg / L respectively, adjust the pH to 5.8, and after sterilizing at 121°C for 20min, dispense them into culture bottles on an ultra-clean workbench with a medium thickness of 2cm; b. Selection and sterilization of explants Bacteria: Select the Polygonatum plants with good growth and robust growth, pick the young leaves that have not fully expanded or just expanded as explants, wash them with running tap water for 1 minute, remove dust and impurities, and then soak them in 0.5% washing powder water for 6 Minutes, rinse with tap water, then soak in 75% ethanol for 20s, rinse with sterile water four times, put into 0.1% mercuric chloride for disinfection for 8min, and finally rinse with sterile water for 5 t

Embodiment 2

[0011] Embodiment 2: a kind of Rhizoma Polygonatum one-time seedling-growing method of the present invention is characterized in that containing following steps: a. the configuration of culture medium: add 6-BA and 2,4-D in MS basic culture medium, and make its concentration Reach 2mg / L and 0.5mg / L respectively, adjust the pH to 6.0, and after sterilizing at 121°C for 20min, dispense them into culture bottles on an ultra-clean workbench with a medium thickness of 3cm; b. Selection and sterilization of explants Bacteria: Select a well-growing and robust Rhizoma Polygonatum plant, pick the young leaves that have not fully expanded or just expanded as explants, rinse with running tap water for 3 minutes to remove dust and impurities, and then soak them in 0.5% washing powder water for 8 Minutes, rinse with tap water, then soak in 75% ethanol for 20s, rinse with sterile water four times, put into 0.1% mercury liter for disinfection for 10min, and finally rinse with sterile water f

Embodiment 3

[0012] Embodiment 3: a kind of Rhizoma Polygonatum one-time seedling-growing method of the present invention is characterized in that containing following steps: a. the configuration of medium: add 6-BA and 2,4-D in MS basic medium, and make its concentration Reach 2mg / L and 0.5mg / L respectively, adjust the pH to 5.9, and after sterilizing at 121°C for 20min, dispense them into culture bottles on an ultra-clean workbench with a medium thickness of 3cm; b. Selection and sterilization of explants Bacteria: Select the Polygonatum plants with good growth and robust growth, pick the young leaves that have not fully expanded or just expanded as explants, wash them with flowing tap water for 2 minutes, remove dust and impurities, and then soak them in 0.5% washing powder water for 7 Minutes, rinse with tap water, then soak in 75% ethanol for 20s, rinse with sterile water four times, put into 0.1% mercuric chloride for disinfection for 9min, and finally rinse with sterile water for 7

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a one-time seedling growing method for polygonatum sibiricum. The method includes the first step of culture medium preparation, the second step of explant selection and sterilization, the third step of explant inoculation, the fourth step of culture, and the fifth step of transplanting and hardening. The one-time seedling growing method for polygonatum sibiricum is convenient and fast to implement. Compared with a traditional method that underground rhizomes are selected for tissue culture, tender leaves of polygonatum sibiricum are selected as explants for tissue culture, materials are easy to obtain, and the economic benefit brought by rhizomes of polygonatum sibiricum is not influenced; besides, as the number of leaves of polygonatum sibiricum is large and the propagation coefficient is higher, the production method is convenient and fast to implement, and tissue culture seedlings of polygonatum sibiricum can be cultured within a short time. In addition, the method is easy and convenient to implement, only one step is needed from explants to seedlings, manual work is greatly reduced, and contamination is reduced to the maximum; moreover, by selecting a fine female parent for germplasm purification and rejuvenation of polygonatum sibiricum and maintenance of superior characters, the method has high application value on propagation of fine seedlings of polygonatum sibiricum.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner 恩施州源惠科技开发有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap