41results about "Plant tissue culture" patented technology

The present invention relates to the abacterial seeding and tissue culture of Paphiopedilum. The Dendrobium is one kind of national first order protected plant and is hard to germinate naturally owing to its incomplete development of embryo. The present invention provides technology of abacterial seeding and tissue culture of Paphiopedilum to obtain great amount of test tube seedling via utilizing artificial nutritious liquid and abacterial seeding, proliferate small seedlings and obtain complete plants via rooting culture. The said seedling growing technology has the advantages of high germination rate, fast seedling growing rate, high seedling quality, etc.

This invention is a new floral preservative that utilizes a novel formulation of using a combination of known and readily available over the counter products that, when sprayed onto the flower as directed, approximately doubles the vase life of fresh cut flowers or preserves flowers to a pliable, non crumbly state approximating their naturally vibrant colors. It is applied to the exterior of the fresh cut flowers in a misting apparatus, therein simplifying the art of preserving flowers.

The invention discloses an in-vitro rapid propagation method of rare and endangered plant ammopiptanthus mongolicus, belongs to the biological field and aims to solve the problems of severe browning and differentiation difficulty in callus induction in existing methods. The in-vitro rapid propagation method of the ammopiptanthus mongolicus comprises steps of obtaining of ammopiptanthus mongolicus explants, induction culture of cluster buds, extension culture of the cluster buds, rooting culture and expansion propagation. Compared with existing in-vitro culture methods of the ammopiptanthus mongolicus, the method has the advantages that cotyledonary nodes are directly subjected to induction of cluster buds and rooting is performed, callus cannot be produced in the process, thus, the step of callus induction is omitted, the cotyledonary nodes are directly cultured into adventitious buds, the in-vitro culture time is greatly shortened, and the whole process from seed germination, obtaining of cotyledonary nodes, induction and extension of the cluster buds to rooting only takes 79 d.

The invention discloses a culture method for a brassica oleracea L. var. acephala microspore regeneration plant. The method comprises the following steps: taking inflorescences of brassica oleracea L. var. acephala and rape, directly taking or taking after induction alabastrum of the inflorescences, adding the alabastrum into NLN-13 induced medium after disinfection to form fluid suspension, carrying out filtering, and centrifuging the filtrate to obtain a precipitate; adding NLN-13 induced medium and active carbon mixed liquor in order so as to obtain a microspore fluid suspension; carrying out a heat shock treatment, followed by culturing so as to obtain embryoids in cotyledon stage; inoculating the embryoids to embryoid differential medium until the embryoids are differentiated and regenerates into buds; cutting the regenerated buds to a rooting medium for rooting culture, and hardening and transplanting seedlings to obtain regenerated plants; taking young leaves of the regenerated plants for the detection of ploidy of corresponding regenerated plants and determining regenerated seedlings of rape and brassica oleracea L. var. acephala in the regenerated plants. According to the method provided in the invention, rape which is easy to generate embryos and brassica oleracea L. var. acephala which is difficult to generate embryos are mixedly cultured; the material which is easy to generate embryos is used to spur the material which is difficult to generate embryos; therefore the ratio of embryos of brassica oleracea L. var. acephala is improved.

The invention discloses a culture method of an ellcalyplus grandis Hill ex Maid tissue culture seedling, which comprises the following operation steps of taking an ellcalyplus grandis Hill ex Maid tissue culture seedling leaf as an explant, inducing a callus tissue of the ellcalyplus grandis Hill ex Maid leaf, and performing adventitious bud differentiation culture and rootage culture on the callus tissue of the leaf, and is characterized in that the callus tissue of the ellcalyplus grandis Hill ex Maid leaf is induced under the irradiation of red light and blue light, the adventitious bud differentiation culture is performed on the callus tissue of the leaf under the irradiation of the red light or the blue light, adventitious bud growth culture is performed under the irradiation of green light, and then the callus tissue is transferred to be under LED (light-emitting diode) yellow light for the rootage culture. According to the method, light sources are adjusted timely according to difference in requirements of the ellcalyplus grandis Hill ex Maid leaf on the light in different tissue culture stages, and the induction rate, the adventitious bud differentiation rate and the rootage rate of the callus tissue are increased effectively. In addition, the method is low in energy consumption, and can greatly increase the yield of the ellcalyplus grandis Hill ex Maid tissue culture seedling and effectively shorten the seedling emergence time in comparison to the traditional tissue culture method.

The invention relates to an isolated culture method of an unfertilized ovary of lilium, belonging to the technical field of plant haploid culture. The method provided by the invention comprises the following steps: with a flower bud one day before bloom as a testing piece, sterilizing and taking the ovary; transversely cutting the testing piece to sheets; then, inoculating a BDS induction medium with the ovary sheets to induce embryogenesis so as to obtain the embryo rate with high frequency, wherein averagely each ovary contains 108-223 embryo ovules; and then, transferring the induced ovary sheets to the BDS regeneration medium to obtain a regeneration plant with other ploidy such as ploidy, diploid and aneuploid through the path of embryogenesis. The method provided by the invention has important meaning for accelerating lilium breeding, germplasm innovation and basic research of genetics.

The invention relates to a one-time seedling growing method for polygonatum sibiricum. The method includes the first step of culture medium preparation, the second step of explant selection and sterilization, the third step of explant inoculation, the fourth step of culture, and the fifth step of transplanting and hardening. The one-time seedling growing method for polygonatum sibiricum is convenient and fast to implement. Compared with a traditional method that underground rhizomes are selected for tissue culture, tender leaves of polygonatum sibiricum are selected as explants for tissue culture, materials are easy to obtain, and the economic benefit brought by rhizomes of polygonatum sibiricum is not influenced; besides, as the number of leaves of polygonatum sibiricum is large and the propagation coefficient is higher, the production method is convenient and fast to implement, and tissue culture seedlings of polygonatum sibiricum can be cultured within a short time. In addition, the method is easy and convenient to implement, only one step is needed from explants to seedlings, manual work is greatly reduced, and contamination is reduced to the maximum; moreover, by selecting a fine female parent for germplasm purification and rejuvenation of polygonatum sibiricum and maintenance of superior characters, the method has high application value on propagation of fine seedlings of polygonatum sibiricum.

The invention discloses a tissue culture method of graptopetabum paraguayense, relates to a cuttage method of a plant, and belongs to the field of plant cultivation methods. The method is characterized by comprising the following steps: scissoring simple leaf, branch stem or telome, drying pruning wound, smearing 1000ppm of naphthylacetic acid on the pruning wound; transplanting on a dedicated sand bed. The manufacturing method is as follows: washing and screening normal sand to leave 100-mesh of sand, mixing 80% of sand, 10% of soil and 10% of manure, covering a plastic film, tightly closing the greenhouse for 15-20 days, preparing the sand for later use. The cuttage method has the beneficial effects that the root of the graptopetabum paraguayense in the cuttage method disclosed by the invention has no rot phenomenon, and the survival rate is improved to 95%; a plant growth regulator and micronutrient element relate to the root development are used as main agents, the root differentiation time can be shortened, the problem of the graptopetabum paraguayense cuttage technology in application is solved.

The invention relates to a quality walnut tissue culture quick propagation method which comprises, appending right amount of iron ions and zinc ions into chestnut culture medium, adjusting nutrient content of culture medium, screening the most suitable culture medium for chestnut growth, selecting right explant for tissue culture at right stage, readjusting the culture medium formulation so as to grow shoot and root for the chestnut, transplanting it into warm house and planting into big nourishing cup equipped with nourishment base materials, finally planting and transplanting the nursery stock in the nourishment cup.

The invention relates to a proliferation culture medium formula in blueberry tissue culture, which consists of the components with different concentrations as follows: macroelements, microelements, iron salts, organic compositions and the like. By using the culture medium, the proliferation efficiency can be as high as more than 15 times; and the proliferation culture time is as short as only 25-30 days, and proliferation seedlings can reach 3-4cm in height, thus fully satisfying the need for rooting culture. Simultaneously, the culture medium consists of the compositions which have common sources without compounds with special forms, and has easy preparation.

The invention provides a cucumber wilt resistance breeding method. The breeding method comprises the following steps: S1 selecting a breeding main parent and a resistant source parent; S2 performing cross breeding; S3 preparing a crude toxin extracting solution; S4 respectively performing anther induction culture on the cucumber breeding main parent and the resistant source parent; S5 determiningthe crude toxin screening concentration; S6 performing anther culture on a hybrid F1 generation; S7 transplanting flower culture seedlings after rooting; S8 performing wilt resistance identification and offspring screening. Hybrid breeding and a haploid breeding technology are combined to significantly improve the cucumber wilt resistance breeding efficiency and shorten the breeding period, and anewly developed cucumber wilt resistant line is good in resistance and further excellent in agronomic trait; while the yield is ensured, the application amount of pesticides and reduce environmental pollution can be reduced; the method is an effective way to rapidly breed new varieties resistant to cucumber wilt.

The invention provides a tissue culture method for improving the survival rate of bananas. The steps are as follows: (1) induction culture; (2) subculture; (3) rooting culture; (4) seedling hardening. And provided three culture media and culture conditions used. Applying the method of the invention to carry out tissue culture on bananas, the survival rate of bananas is as high as over 91%.

The invention discloses a novel material and a method for relieving heavy metal stress of crambe. The material and the method are characterized in that the novel material is sulfur nanoparticles (SNPs), and the method includes adding a suspension of the sulfur nanoparticles into a medium containing heavy metals for planting crambe, wherein the SNPs concentration is 50-300mg/L, the suspension is that the sulfur nanoparticles are added into sterile water and ultrasonically dispersed for 40-60 minutes in a sterile environment, the particle diameter of the sulfur nanoparticles is 30-50nm, and thepurity is 99.99%. The method can effectively relieve the toxicity of heavy metals to the crambe and obviously promote the growth of the crambe under the stress treatment of As(V), Hg2+ and Pb2+.

The invention relates to a rapid breeding method for the tissue culture of radix ophiopogonis. The method comprises the steps of obtaining sterile leaves of the radix ophiopogonis, regenerating the leaves, carrying out rooting culture, carrying out hardening-seedling seedlings, and transplanting. The radix ophiopogonis prepared by adopting the method is simple in production technology, high in survival rate and low in production cost; the method is beneficial to large-scale production of the radix ophiopogonis.

A soybean cultivar designated 70140849 is disclosed. The invention relates to the seeds of soybean cultivar 70140849, to the plants of soybean cultivar 70140849, to the plant parts of soybean cultivar 70140849, and to methods for producing progeny of soybean cultivar 70140849. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 70140849. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 70140849, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 70140849 with another soybean cultivar.

The invention relates to a method for cultivating transgenic melanin strawberries, which belongs to the field of planting technology. The method uses black soybean pigment gene, culture medium, sodium thiosulfate, magnesium sulfate, kudzu root dregs, starch, sodium sulfate, hypostone, and water as Raw materials, through gene transfer technology, the black soybean pigment gene extracted from black soybeans is injected into the embryos of strawberry seeds, and a compound organic nutrient solution containing potassium, iron and other trace elements is added to the soil to cultivate a vitamin-rich E. Black strawberries with iron elements. The appearance of the strawberry is black, the particles are large and uniform in size; rich in vitamin E and iron, which are easily absorbed by the human body, have a high biotransformation rate, and have special curative effects on patients with cerebral thrombosis; It has strong resistance to adversity and can be stored for 25 to 30 days at a low temperature of -5 to -10°C.

Process for obtaining genetically modified plants, especially woody plants, especially plants of the genus Eucalyptus and Pinus, comprising transforming target plant material with a desired genetic construct and transforming transformed plant material using an adventitious shoot system The material is recycled. The present method has high transformation efficiencies and significantly reduces the duration of transformation and regeneration protocols. Stem nodes of target plants were transformed by Agrobacterium-mediated technology, and adventitious shoots were regenerated from stem nodes infected with Agrobacterium. There are also preferred culture media, including selection media, and improved plant culture techniques.

The invention discloses an ailanthus altissima efficient in-vitro culture plant regeneration method. The method comprises the procedures of explant selection, explant treatment, initial bud induction culture, multiplication culture, strong bud culture and rooting culture, and comprises the following specific steps: collecting a semi-lignified robustly-growing annual ailanthus altissima shoot to serve as an explant; trimming the explant, sterilizing and disinfecting the trimmed explant, and inoculating the sterilized and disinfected explant into an initial bud induction culture medium to obtain an initial bud; inoculating the initial bud into a multiplication culture medium, and performing multiplication culture for 3 to 4 periods to form cluster buds; inoculating the cluster buds into a bud strengthening culture medium for culturing; lastly, inserting single buds which are more than or equal to 2cm in heights into the rooting culture medium to induce rooting. By adopting the method, the ailanthus altissima seedling period is shortened, the seedling material is saved, the problems of need of a large quantity of seedling materials, long period and the like in the conventional seedling method are solved, the production cost is lowered greatly, the production efficiency is increased, and good economical benefit, social benefit and ecological benefit are achieved.

The invention discloses a high-efficiency, rapid and low-cost method of obtaining cassava tissue culture seedlings. The method comprises the steps of A, preparation of a semisolid culture medium; B, surface disinfection of cassava explants; C, starting culture and survival of the tissue culture seedlings. The method has the advantages that components of a traditional culture medium are reduced onthe basis of ensuring that the cassava tissue culture seedlings can grow normally, the cassava tissue culture seedlings can also be obtained in a short time, the growth vigor of the tissue culture seedlings is more normal, and the cost for asexual reproduction of cassava is reduced.

The invention discloses a method for rapidly screening a low-temperature and weak-light breeding material of cucumis melo L.var.conomon Group, and relates to the technical field of genetic breeding. Firstly, embryo fetching conditions of the crisp melons are determined, wherein 16-18 days after flowering and pollination, peeled seeds obtained under the conditions have a high survival rate after being subjected to tissue culture and germinate 10 days earlier than mature seeds; secondly, the best culture medium for the peeled seeds is determined, wherein the culture medium contains 950 mg/L potassium nitrate, 825 mg/L ammonium nitrate, 85 mg/L potassium dihydrogen phosphate, 90 mg/L anhydrous magnesium sulfate, 165 mg/L anhydrous calcium chloride, 0.83 mg/L potassium iodide, 6.2 mg/L boric acid, 16.9 mg/L manganese sulfate monohydrate, 8.6 mg/L zinc vitriol, 0.25 mg/L sodium molybdate dihydrate, 0.025 mg/L copper sulfate pentahydrate, 0.025 mg/L cobalt chloride hexahydrate, 37.26 mg/L ethylene diamine tetraacetic acid disodium, 15.2 mg/L anhydrous ferrous sulfate, 8,500 mg/L agar powder, 18,000 mg/L sucrose and the like; meanwhile, the best conditions for adversity identification when obtained seedlings of cucumis melo L.var.conomon Group are cultured to the stage of cotyledon unfolding are determined, wherein the daytime temperature is 15-18 DEG C, and the night temperature is 12-15 DEG C. By means of the method, the target material can be rapidly screened.