Aspirin-containing banana rooting-promoting culture medium

[0020] Prepare the 1 / 2MS medium of the experimental group according to the following steps:

[0021] Step S10, using an electronic scale to prepare a 1000-fold 1 / 2MS medium stock solution according to the 1 / 2MS medium standard formula;

[0022] Step S20, take 1 / 2MS medium mother liquor to prepare 1 / 2MS medium, and add PP according to the formula 333 (Paclobutrazol), lanthanum nitrate, aspirin solution, NAA (naphthalene acetic acid), agar powder and white sugar, use hydrochloric acid or sodium hydroxide to adjust the pH of the medium;

[0023] Step S30, sterilize the culture medium at 121°C for 20 minutes in a high-temperature and high-pressure steam sterilizer, take out the culture medium and leave it to cool in the ultra-clean workbench;

[0024] Step S40, prepare a vitamin solution according to the formula, and filter and sterilize it;

[0025] In step S50, when the medium is cooled to 60°C, the reagent prepared in step S40 is added to the medium, shake evenly, stand still, and cool a...

[0029] The difference between the second embodiment and the first embodiment is that the concentration of the medium formula is adjusted.

[0030] Prepare the 1 / 2MS medium of the experimental group according to the following steps:

[0031] Step S10, using an electronic scale to prepare a 1000-fold 1 / 2MS medium stock solution according to the 1 / 2MS medium standard formula;

[0032] Step S20, take 1 / 2MS medium mother liquor to prepare 1 / 2MS medium, and add PP according to the formula 333 (Paclobutrazol), lanthanum nitrate, aspirin solution, NAA (naphthalene acetic acid), agar powder and white sugar, use hydrochloric acid or sodium hydroxide to adjust the pH of the medium;

[0033] Step S30, sterilize the culture medium at 121°C for 20 minutes in a high-temperature and high-pressure steam sterilizer, take out the culture medium and leave it to cool in an ultra-clean workbench;

[0034] Step S40, prepare a vitamin solution according to the formula, and filter and sterilize it;

[0035] In s...

[0039] Prepare the 1 / 2MS medium of the experimental group according to the following steps:

[0040] Step S10, using an electronic scale to prepare a 1000-fold 1 / 2MS medium stock solution according to the 1 / 2MS medium standard formula;

[0041] Step S20, take 1 / 2MS medium mother liquor to prepare 1 / 2MS medium, and add PP according to the formula 333 (Paclobutrazol), lanthanum nitrate, aspirin solution, NAA (naphthalene acetic acid), agar powder and white sugar, use hydrochloric acid or sodium hydroxide to adjust the pH of the medium;

[0042] Step S30, sterilize the culture medium at 121°C for 20 minutes in a high-temperature and high-pressure steam sterilizer, take out the culture medium and leave it to cool in the ultra-clean workbench;

[0043] Step S40, prepare a vitamin solution according to the formula, and filter and sterilize it;

[0044] In step S50, when the medium is cooled to 60°C, the reagent prepared in step S40 is added to the medium, shake evenly, stand still, and cool a...