80 results about "Culture mediums" patented technology

The invention relates to an edible mushroom, spent mushroom substrate and probiotics-containing nutrient solution for livestock and poultry and a preparation method thereof. The preparation method comprises the following steps of: preparing leach liquor by using waste spent mushroom substrates as raw materials, extracting a plurality of active ingredients from the spent mushroom substrates, and then fermenting and preparing the nutrient solution containing a plurality of microorganisms beneficial for the growth of the livestock and poultry by using the leach liquor as a culture medium. The prepared nutrient solution is brown in color and tart, slightly has the incenses of edible mushrooms, and contains abundant probiotics groups so as to be beneficial for enhancing the appetite of the livestock and poultry and promoting the absorption of nourishment and the growth of the livestock and poultry. Compared with the solid fermentation of the conventional preparation method of spent mushroom substrate-containing feeds, due to the adoption of the main production method of liquid fermentation, the preparation method of the nutrient solution has the advantages of environmental protection, energy saving and convenient operation; and the obtained product is liquid so as to have the advantages of convenient application and favorable palatability for the livestock and poultry.

The invention discloses a method for producing astaxanthin by inducing Haematococcus pluvialis, which is characterized by comprising the following steps: in the vegetative growth phase, inoculating Haematococcus pluvialis in a nutrient culture medium, and culturing; continuously keeping irradiation on the Haematococcus pluvialis and ensuring that the intensity of illumination arriving at the Haematococcus pluvialis cell surface is 20-200 mu mol.m<-2>.s<-1>; in the induction stage, adding ethanol and seawater crystal into the production culture medium, wherein the ethanol added into the production culture medium accounts for 0.5-3 vol%, and the seawater crystal added into the production culture medium accounts for 0.5-1 wt%; and continuously introducing filtered air mixed with 1-3 vol% carbon dioxide into the production culture medium, wherein 0.2-0.4L/minute of the air is introduced to every 1L of production culture medium. The method can induce the Haematococcus pluvialis to quickly accumulate the astaxanthin, is simple and has the advantages of high yield and favorable effect.

The invention provides a selenium-enriched mushroom grass culture medium which is prepared by taking selenium-enriched mushroom grass, wood chips, wheat bran, saccharose, gypsum, monopotassium phosphate and magnesium sulfate as the main raw materials. The invention further provides a method for producing shiitake mushrooms through the selenium-enriched mushroom grass culture medium. Accordingly, a traditional shiitake mushroom producing method is changed, a selenium-enriched mushroom grass technology is utilized in shiitake mushroom stick production, therefore, the producing method of selenium-enriched products is changed, the nutritional value of the shiitake mushrooms is increased, the production efficiency is improved, the shiitake mushroom yield and biotransformation rate are high, and meanwhile the production cost is greatly lowered; in addition, the producing method is simple in technology, the produced shiitake mushrooms are high in content of selenium-enriched elements, the product is free of pollution, and the food safety problem is solved from the source.

The invention relates to a preparation method of an antioxidant. The preparation method of the antioxidant provided in the invention comprises the following steps: culturing plant lactobacillus in a culture medium containing soybean residues, and obtaining the antioxidant from the resultant supernatant. The invention also provides the antioxidant having a high SOD activity obtained through the preparation method. The antioxidant is obtained through utilizing a specific microbe and adopting the byproduct soybean residues of the food processing industry and the like as a main raw material of the medium according to the preparation method provided in the invention, and the preparation method has the advantages of low cost and high efficiency.

The invention discloses Escherichia coli and a method for preparing L-cysteine by using the same. Escherichia coli BL21(DE3) is used as a template for the genome of the Escherichia coli, and the template has serine acetyltransferase shown as a sequence in SEQ ID NO.1 and in deletion of a gene metC, a gene tnaA, a gene gshA and a gene dfp. The method comprises the following steps of: firstly, putting the Escherichia coli in a seed culture medium for culturing; and then putting the Escherichia coli in a fermentation culture medium to ferment to obtain the L-cysteine. The invention has the advantages that by utilizing the Escherichia coli to prepare the L-cysteine, the conversion rate of a substrate and the output of the L-cysteine are greatly improved; the conversion rate of the substrate can reach 30 percent improved by more than 50 percent compared with that of the prior art; and the output of the L-cysteine can be improved by more than 20 percent compared with that of the prior art.

The invention discloses an improved culture medium for neonatal pig islet cells and a use method of the same. An apoptosis inhibitor Z-VAD-FMK, a human basic fibroblast growth factor hFGF-beta, a vascular endothelial growth factor VEGF, an insulin-like growth factor R3-IGF-1, a human epidermal growth factor hEGF, a hepatocyte growth factor hHGF, pig serum, sodium selenite, insulin, transferrin, ethanol amine, 1-methyl 3-isobutyl xanthine, nicotine, cortisol, penicillin and streptomycin are added in HAM'S F10 culture medium. According to the culture medium, apoptosis can be reduced and recovery rate can be increased; the cultured cells have a sensitive glucose stimulation response and more mature functions compared with generally-cultured islet cells; the insulin secretion amount of the islet cells with the same quantity is increased; the viability of islet beta-cells is improved; the breakages of islet cell clusters are reduced, and the envelops of the cell clusters are complete, so that the cell clusters are more suitable for being transplanted.

The invention discloses a culturing method for the mycelia of Antrodia camphorata, belonging to the field of biological fermentation technology. The method of the invention comprises the following steps: inoculating a slant culture medium with a Antrodia camphorata strain for activation and purification so as to obtain an activated mother strain; inoculating a liquid shake-flask culture medium with the activated mother strain for static culture, and carrying out stirring culture after mycelia germinate and are free of pollution so as to obtain a shake-flask strain; inoculating a fermentation medium with the shake-flask strain for fermentation culture so as to obtain fermented mycelia; and inoculating a artificially synthesized solid culture medium with the fermented mycelia which are usedas a liquid strain and carrying out fermentation for 30 to 90 days so as to obtain the mycelia of Antrodia camphorata. The method of the invention has the advantages of a short cultivation period forthe mycelia of Antrodia camphorata, a high culture success rate of and high medicinal value of the mycelia.

The invention discloses a preparation method of probiotics milk powder. The preparation method comprises the following steps: 1), raw milk treatment: carrying out an ultra-temperature instant sterilization treatment to on the raw milk and then carrying out vacuum depression concentration to obtain concentrated milk; 2), bacteria cultivation: inoculating probiotics to sterilized fresh milk or enrichment medium to obtain a bacteria nutrient solution, wherein the volume of the inoculated probiotics is 1-3% of the volume of the bacteria nutrient solution; 3), homogenization: adding 1-10% of bacteria culture in percentage by weightmass fraction to the concentrated milk in the step 1) for homogenizing to obtain a concentrated milk mixture containing probiotics; and 4), spraying and drying the concentrated milk mixture containing the probiotics to obtain the milk powder containing probiotics. The preparation method disclosed by the invention has a simple operation process, low device requirements, saves cost and is suitable for large-scale production. The probiotics milk powder prepared from by the preparation method provided by the invention has all nutrition of common milk powder, and further has health benefits of various probiotics.

The invention discloses a lactobacillus paracasei as well as a strain domestication method and a culture method thereof. The lactobacillus paracasei is capable of both ensuring that a selenium-containing state refers to organic selenium, and taking physiological functions of probiotics into play. The strain domestication method of the lactobacillus paracasei comprises the following steps: inoculating the lactobacillus paracasei with a lactobacillus paracasei seed culture medium; performing culture; when the OD (Optical Density) value of a culture liquid is up to 1.2-1.6, recording the culturetime as h1; adding a sodium selenite liquid till the content of selenium in the culture liquid is 0.2-1mu g/g; and further performing domestication culture for the time of h1. The culture method of the lactobacillus paracasei comprises the following steps: inoculating a lactobacillus paracasei strain obtained through domestication to a liquid potato culture medium of selenium-enriched potatoes; performing culture; when the OD value of the culture liquid inside the liquid potato culture medium is up to 1.2-1.6, recording the culture time as h2; adding a sodium selenite liquid till the content of selenium in the culture liquid is 0.6-10mu g/g; and further performing domestication culture for the time of h2.

The invention discloses a method for culturing oleaginous microalgae. The method comprises the steps of: inoculating the oleaginous microalgae at a logarithmic growth period into a culture medium containing an organic carbon source, culturing under the alternative circulation environment of irradiation and no irradiation, and culturing until at a platform period. According to the method, cultivation is performed under the alternative circulation environment of irradiation and no irradiation, so that the important role for fixing carbon dioxide is also taken into account while the biomass and lipid yield is guaranteed, and the period of microalga cultivation is greatly shortened.

The invention relates to a making method of a high-protein fermented feed, belongs to the technical field of feed processing, and particularly relates to a making method of the high-protein fermented feed. The making method comprises the following steps of weighing a certain mass of linseed meal, adding sprouted cargo rice powder and ammonium sulfate, adding water and performing uniform mixing so as to obtain a solid culture medium; and enabling the solid culture medium to be subjected to enzymolysis, sterilization, fermentation, low temperature drying, crushing, granulation and packaging, so as to obtain finished products. The high-protein fermented feed disclosed by the invention has the advantages of being rich in nutrition, high in protein content, and good in palatability. In addition, the step of enzymolysis is performed before fermentation, so that nutrition resistant factors having toxicity in the linseed meal are decomposed and released, the eating safety is improved, nutrient substances having health-care efficacies, such as gamma-aminobutyric acid and flavonoid glycosides are also generated, and the health-care efficacies of the fermented feed are improved.

The invention discloses a culture medium for hepatoma organ cell sphere culture. The culture medium is composed of a basic culture medium, 10-25 ng/mL of an epidermal growth factor, 10-25 ng/mL of a fibroblast growth factor, 0.5-2 ng/mL of a cell growth factor, 1-3 ng/mL of an insulin growth factor, 1 mmol/L of sodium pyruvate, 2 mmol/L of glutamine, 100 U/mL of penicillin, 100 [mu]g/mL of streptomycin and 10% by volume of serum. Aiming at the culture growth characteristics of liver cancer tissue-derived cells, the culture medium provided by the invention is simple and reasonable in components, rich in nutrition and moderate in formula price; and hepatoma organ cell spheres cultured by the culture medium can effectively and stably grow for a long time, are closely gathered, have smooth andround surfaces and clear boundaries, is free of disintegration phenomenon, and can be significantly improved in cell activity, specific functions and metabolic functions.

The invention relates to a method for biologically synthesizing natural gas from coke oven gas. The method comprises the following steps: (1) introducing organic wastes, the coke oven gas and aerobic activated sludge into a sealed deoxidization bio-reactor to perform a microaerobic biological deoxidization reaction; (2) introducing a nitrogen-free culture medium, the deoxidized coke oven gas and free-living anaerobic nitrogen-fixing bacteria into a sealed denitriding bio-reactor to perform an anaerobic biological denitriding reaction; (3)introducing the organic wastes subjected to the microaerobic biological deoxidization reaction from the sealed deoxidization bio-reactor, and a biogas producing inoculum into a sealed biogas producing reactor to perform biogas production by anaerobic fermentation; (4) performing solid-liquid separation on fermentation residues after biogas production by anaerobic fermentation, wherein a filtered biogas liquid contains rich nutriments and serves as a culture medium for subsequent methane production by anaerobic fermentation; and (5) introducing the deoxidized/denitrified coke oven gas, the biogas, the filtered biogas liquid and methane producing bacteria into a sealed methane producing reactor to perform methane production by anaerobic fermentation.

The invention relates to an automatic root peeler for Pholiota nameko, which is a food initial machining machine capable of cutting of an aging root of the end part of Pholiota nameko stem, and culture medium residues. The automatic root peeler for the Pholiota nameko scatters root-peeled Pholiota nameko by utilizing two-stage conveyor belts with different conveyance speeds, sorts and locates by utilizing the characteristics of self appearances of a V-shaped slot and the Pholiota nameko, and finally realizes continuous cutting by utilizing a cutter; and workers only need to pour the Pholiota nameko picked from a culture medium onto the conveyor belts. Compared with a present manual root peeling method, the automatic root peeler for the Pholiota nameko, disclosed by the invention, has the advantages of saving labor and improving production efficiency.

The invention relates to a one-time seedling growing method for polygonatum sibiricum. The method includes the first step of culture medium preparation, the second step of explant selection and sterilization, the third step of explant inoculation, the fourth step of culture, and the fifth step of transplanting and hardening. The one-time seedling growing method for polygonatum sibiricum is convenient and fast to implement. Compared with a traditional method that underground rhizomes are selected for tissue culture, tender leaves of polygonatum sibiricum are selected as explants for tissue culture, materials are easy to obtain, and the economic benefit brought by rhizomes of polygonatum sibiricum is not influenced; besides, as the number of leaves of polygonatum sibiricum is large and the propagation coefficient is higher, the production method is convenient and fast to implement, and tissue culture seedlings of polygonatum sibiricum can be cultured within a short time. In addition, the method is easy and convenient to implement, only one step is needed from explants to seedlings, manual work is greatly reduced, and contamination is reduced to the maximum; moreover, by selecting a fine female parent for germplasm purification and rejuvenation of polygonatum sibiricum and maintenance of superior characters, the method has high application value on propagation of fine seedlings of polygonatum sibiricum.

The invention discloses a biological reader capable of rapidly verifying the sterilizing effect. The biological reader mainly comprises an excitation light source, a fluorescent light filtering disc system, an optical signal acquisition system, a fluorescent biological indicator, a temperature control system, an image processing system and an output result; the main principle and the working process of the biological reader are as follows: a biological indicator after sterilization is inserted into the biological reader, spores germinate in a culture solution at appropriate temperature under the action of a germination accelerator, an active enzyme is generated and reacts with an added fluorescent material in a culture medium, the excitation light source emits excitation light with the specific wavelength and irradiates the fluorescent biological indicator, the fluorescent material in the fluorescent biological indicator can emit fluorescent light with the specific wavelength after excitation, the fluorescent light is captured and imaged by a high-sensitivity digitized CCD, and an image processing system can process, compare and analyze acquired image information and judge whether the sterilization is effective within a short time.

The invention discloses a propagation method of fir woodland predominant endomycorrhizal fungi, belonging to the technical field of forest conservation. A pot-culture method is utilized to reproduce the fir woodland predominant endomycorrhizal fungi: the host plant is sorghum; the culture medium is composed of sand, vermiculite and soil according to the volume ratio of 2:1:1; the culture density is 20 plants/5L pot, and each pot is filled with 2.5kg of the medium containing spores of the fir woodland predominant endomycorrhizal fungi; and a 30% Hoagland's nutrient solution is applied once a week from the emergence of seedlings, and the application is stopped after 12 weeks, wherein 100mL is applied for each pot at first, and 200mL is applied for each pot from the eighth week. The method can effectively reproduce the propagules of the fir woodland predominant endomycorrhizal fungi.

The invention relates to a proliferation culture medium formula in blueberry tissue culture, which consists of the components with different concentrations as follows: macroelements, microelements, iron salts, organic compositions and the like. By using the culture medium, the proliferation efficiency can be as high as more than 15 times; and the proliferation culture time is as short as only 25-30 days, and proliferation seedlings can reach 3-4cm in height, thus fully satisfying the need for rooting culture. Simultaneously, the culture medium consists of the compositions which have common sources without compounds with special forms, and has easy preparation.