26 results about "Cellulase" patented technology

The invention discloses a mushroom zymolyte and application thereof to the preparation of a mushroom essence. The mushroom zymolyte is prepared by using mushroom as a raw material through enzymolysis of a cellulase and a papain, wherein the weight ratio of the cellulase to the papain is (0.5-1):(0.3-0.5), the enzymolysis temperature is 45-55 DEG C, and the enzymolysis time is 3-5h. The mushroom essence prepared by using the mushroom zymolyte as a precursor through a Maillard reaction is strong in naturality, good in naturalness, full in flavor, refresh and delicious in taste and remarkable in wood scent, faint scent and fresh scent of the mushroom.

The invention discloses an extraction method of ginger essential oil. The method comprises the following steps: preparing ginger powder: slicing fresh ginger, soaking the sliced ginger in a 0.5-0.9wt% starch solution for 10-15min, drying and grinding the sliced ginger, and filtering obtained powder through a sieve of 60-80 meshes; preparing enzymatic hydrolysate: adding a de-ionized liquid 1-ethyl-3-methyl imidazole acetate ([Emim]OAc) to a disodium hydrogen phosphate-citrate buffer solution to obtain an ionic liquid system; adding the ginger powder to the ionic liquid system, controlling a material-to-liquid ratio at (1: 10)g/ml-(1: 20)g/mL, adding cellulase which is 2000-4000U/g in enzyme activity ratio and enzymatically hydrolyzing for 3-5h at 40-50 DEG C; and 3) essential oil extraction: and implementing enzyme deactivation to the system in a water bath after the reaction, ultrasonically extracting for 5-10min, conducting microwave treatment, filtering through gauze, centrifuging, standing and taking the de-ionized liquid on lower layer, spin-steaming, collecting oily matters having intense fragrance and drying the oily matters through anhydrous sodium sulfate to obtain the ginger essential oil.

The invention provides a soil bio-activator for ginseng continuous cropping and belongs to the field of modernization of Chinese traditional medicine. The soil bio-activator for ginseng continuous cropping can be used for solving the problems of serious ginseng continuous cropping diseases, low yield and poor quality caused by deterioration of the physicochemical and biological characters of old ginseng-grown land soil. The soil bio-activator for ginseng continuous cropping is prepared from 2.0%-8.0% of bacillus subtilis, 2.0%-8.0% of actinomycetes, 5.0%-20.0% of xylanase, 5.0%-20.0% of cellulase, 10.0%-20.0% of urease, 1.0%-4.0% of potassium dihydrogen phosphate, 0.1%-0.5% of ferrous sulfate, 0.1%-0.4% of boric acid, 0.1%-0.5% of manganese sulfate, 0.05%-0.2% of copper sulfate, 0.1%-0.4% of zinc sulfate, 0.05%-0.2% of ammonium molybdate and the balance of organic materials through mixing and compositing. The soil bio-activator for ginseng continuous cropping is used for improving the soil for ginseng continuous cropping in ginseng growing.

The invention discloses kelp microcrystalline cellulose and a preparation method thereof as well as application of the prepared microcrystalline cellulose. The preparation method of the kelp microcrystalline cellulose comprises the following steps of by adopting waste kelp residues after kelp glue-extracting as the materials, carrying out flocculation collecting, acidification decalcification, alkaline treatment, bleaching treatment, washing, drying and hydrolytic treatment, wherein in the hydrolytic treatment, two different low-temperature cellulases are respectively added according to the addition of 20U/g-30U/g; carrying out enzymolysis for 0.5-12 hours in a water bath of 10 DEG C-40 DEG C; and drying and crushing to obtain the kelp microcrystalline cellulose. According to the preparation method of the kelp microcrystalline cellulose, the production cost is low, the environment pollution is small, the hydrolysis step adopts a double-enzyme jointed enzymolysis technology, the degradation speed is stable, the degraded cellulose is uniform in molecular weight, the cellulose crystalline grains are small and uniform, degradability of the fibers can be effectively controlled, and the microcrystalline cellulose with high purity and high activity is obtained. The kelp microcrystalline cellulose disclosed by the invention is high in cellulose content, high in water holding capacity and expansion force, and can be used for food additive and pharmaceutical adjuvant in the food industry.

The invention provides an elastic pure cotton woven fabric with smooth surface and soft handle and a preparation method thereof. The method includes the steps of: pretreatment; alkali mercerizing treatment: employing an alkali liquor with a concentration of 20-28 Baume degree to conduct treatment for 45s-60s; liquid ammonia mercerizing treatment; enzyme treatment: putting a fabric in a working solution containing cellulase, and flapping the fabric at the same time; softening treatment: using a ternary polymerization softening agent to perform softening treatment on the fabric; washing and setting treatment; and pre-shrinking treatment, thus obtaining a pure cotton woven fabric finished product. The pure cotton woven fabric obtained by the technical scheme provided by the invention not only has silky gloss, but also has obvious tightness and good resilience in itself, and the handfeel is soft and full. At the same time, the fabric does not contain formaldehyde, and is safe to human body.

The invention provides natural bamboo fibers modified by nano-cellulose whiskers and a preparing method of the natural bamboo fibers. The preparing method specifically comprises the following steps that fresh bamboos are cut off and sliced into bamboo chips, the bamboo chips are steeped into an alkali solution containing cellulase, boiled, taken out, rolled and spin-dried, and pretreated bamboo fibers are obtained; the pretreated bamboo fibers are put into a steam explosion treatment device for steam explosion treatment, taken out, washed with water and filtered, bamboo fiber bundles are obtained, and long natural bamboo fibers and short natural bamboo fibers are obtained through combing; the short natural bamboo fibers are put into an acetic acid solution, ultrasonic crushing is carried out, and a cellulose whisker solution is formed; the long natural bamboo fibers are combed and twisted to form initial-spun fibers, then the initial-spun fibers are steeped into the cellulose whisker solution, ammonium hydroxide is dropwise added, the initial-spun fibers are taken out and subjected to vacuum drying, and the natural bamboo fibers modified by nano-cellulose whiskers are formed.

The invention discloses a cellulase separation and purification method. According to the method, fungus liquid deep layer fermentation cellulase fermentation liquid is subjected to plate frame filtering for removing large particle substances and hyphae in the fermentation liquid, the cellulase plate frame filter liquid is stored into a filter liquid storage tank, in order to avoid the enzyme activity loss, the low-temperature state is maintained, the cellulase plate frame filter liquid is subjected to ultrafiltration membrane concentration and is mixed with protecting agents of lactose and silicon dioxide, the uniform stirring is carried out, the mixed lactose materials pass through bacterium removing membrane equipment for filtering out sundry bacteria, bacterium removing cellulase enzyme material liquid is subjected to the spraying drying process to obtain cellulase powdery finished products. The cellulase powdery finished products separated and purified according to the method provided by the invention solve the problem of great loss of cellulase caused by sundry bacteria generated in the subsequent extraction process of the cellulase fermentation liquid, in addition, after the adoption of the protecting agent spraying, the spraying enzyme activity yield is improved by 18 percent, the cellulase yield of the whole purification process is improved by 23 percent, and the production cost is reduced.

The invention relates to a pulping method of sulphate chemical pulp, which is characterized in that sodium carboxymethylcellulose, i.e. CMC assistant grass sulphate chemical pulp is adopted to pulp, or modified cellulase is adopted to pulp the sulphate chemical wood pulp; CMC is added for pulping to improve the lubricity between fibres, decrease heterocell breakage and fibre cutoff and accelerate the fibrillation of reed pulp fibre when pulping so as to bring convenience for obtaining size with higher tearing index and fracture length; meanwhile, CMC assistant grass sulphate chemical pulp used for pulping can save energy consumption; when 0.125% of CMC is added, 30% of energy consumption is saved; and meanwhile, the modified cellulase pretreats the sulphate chemical wood pulp to effectively improve paper forming strength and save energy consumption by more than 30%.

The invention belongs to the field of functional food, and particularly relates to whitening, moisturizing and anti-saccharification soft sweets and a preparation method thereof. The whitening, moisturizing and anti-saccharification soft sweets are prepared from the following components in parts by mass: 500-900 parts of a sweetening agent, 10-60 parts of a gel agent, 50-200 parts of a functional component and 1-20 parts of an acidity regulator. According to the whitening, moisturizing and anti-saccharification soft sweets, after tremella is ground into pulp, pectinase and hemicellulase are added, the hemicellulase can enzymatically hydrolyze tremella polysaccharide into micromolecular tremella polysaccharide, and the micromolecular tremella polysaccharide is convenient for human body to absorb; and meanwhile, the acidity regulator is added to form glucuronic acid with the micromolecular tremella polysaccharide, and the glucuronic acid can effectively scavenge free radicals, the tremella polysaccharide has gelling property, and when the tremella polysaccharide is matched with the gels, the dosage of the gels can be reduced, so that the obtained soft sweets have good elasticity and hardness.

The invention discloses an actinomycete signal peptide for expressing intracellular protein to an extracellular position. The actinomycete signal peptide is named as Kp-SP, and is from a Kocuria sp.3-3 strain; the nucleotide sequence of the actinomycete signal peptide is shown as SEQ ID No.1, and the amino acid sequence is shown as SEQ ID No.7. The invention also discloses application of the signal peptide to the expression of amylase or cellulose by using the built recombinant escherichia coli. The signal peptide has the capability of secreting foreign protein to the extracellular position in a normal expression carrier, so that the protein expression quantity is increased; the enzyme activity is obviously improved; in addition, the foreign protein extracellular secretion capability is also realized in the constitutive expression carrier pBSPPc; an effective path is provided for producing high-activity secretion protein. As a basic gene engineering technology, the invention provides the favorable conditions for the industrial protein production; the protein expression quantity and the enzyme activity are enhanced; the separation cost is reduced; great application prospects are realized in the protein industrial production aspect.

The invention discloses an application of beta-glucan and succinyl glycan in prevention and control of soil-borne fungal diseases. A method comprises the following steps: adding a mixture of beta-glucan and succinyl glycan into soil; so that the increase of the quantity of streptomyces in soil and the increase of the activity of hydrolase such as beta-glucanase, chitinase and cellulase in the soilare induced, and effectively prevent and control on various soil-borne pathogenic fungus diseases through the combined action of streptomyces and various hydrolases can be realized. The method has the advantages of wide sources of required raw materials, simple operation, better effect than single use of one polysaccharide, and good environmental compatibility.

The invention discloses a feed for enhancing the immunity and disease resistance of poults, which is prepared from the following materials in parts by weight: corn, wheat bran, peanut hull powder, carrot, pumpkin, zucchini, bone meal, shrimp shell meal, table salt, honeysuckle flower, plantain herb, officinal magnolia bark, Chinese atractylodes rhizome, thinleaf buckthorn fruit, common andrographis herb, probiotics, saccharomycete, cellulase and amylase. According to the feed for enhancing the immunity and disease resistance of the poults, since the feed is combined with the traditional Chinese medicine composition, the feed utilization rate is high, the feed can help avian animals to digest feed ingredients which cannot be digested and cannot be completely digested by avian animals, and decompose substances in the feed, such as phytate and polysaccharide, so that nutrition in the feed can be sufficiently released, moreover, the immunity and disease resistance of avian animals can be enhanced, the incidence rate of poultry can be decreased, the growth of the poults can be promoted, and the survival rate of the poults can be increased.

The invention relates to a hydrolysis saccharification process of alternanthera philoxeroides. Metal substance pollutants (iron, cobalt and nickel) in alternanthera philoxeroides raw materials are absorbed by adopting magnetic force, so that subsequent mechanical abrasion and contents of metal ions in treating fluid are reduced, and a good fermentation condition is created for detoxification of selected cupriavidus metallidurans; then solid-liquid separation is realized by adopting an extrusion method, hydrolysis on lipids and protein contained in liquid-state materials is carried out by adopting an NaOH solution, and then hydrolysis treatment is further carried out by adopting trypsase, so that the hydrolysis is more completely carried out; meanwhile, extruded solids are dried and crushedand then dropped into a sulfuric acid solution to carry out acidolysis; through electrorheological fluid shear stress effect treatment, cellulase is then added and enzymatic treatment is carried out,so that the effect is good and energy consumption is low. According to the hydrolysis saccharification process of the alternanthera philoxeroides disclosed by the invention, the raw material treatment efficiency is improved to the maximum extent, and finally, saccharified hydrolytic solutions rich in nutrient substances such as glucose, amino acid, small molecule chain lipids and the like are obtained, so that the glucose content is high, and the glucose can be conveniently supplied for further fermentation, and the production efficiency is improved.

The invention belongs to the technical field of building material preparation, and particularly relates to a preparation method of a high-strength waterproof and antibacterial ceiling plate. The method comprises the following steps: drying and mixing castor-oil plants and cotton, mixing a self-made binder with perlite, kaolin and other raw materials, making a blank, and carrying out glazing and high-temperature firing to obtain the high-strength waterproof and antibacterial ceiling plate. The castor-oil plants and the cotton are subjected to alkali liquor boiling and cellulase enzymolysis to achieve high-temperature hydrolysis of plant fiber components contained in the castor-oil plants and the cotton, and the structural strength of the microstructure of the ceiling plate is improved by utilizing the action of hydroxyl groups and hydrogen bonds, so that the deformation resistance and the strength of the ceiling plate are improved; silver nitrate is introduced into the plant fibers, thedeformation resistance and the durability of the ceiling plate are improved through an enzymolysis reaction, elemental silver nanoparticles are dispersed in the ceiling plate to achieve good sterilization and antibiosis performances, and the plant fibers generate carbon fibers under the high-temperature condition to improve the compactness of the ceiling plate and enhance the waterproof performance, so the ceiling plate has wide application prospects.

The invention relates to a microorganism and enzyme mixed preparation for stripping dirt of aquatic plants as well as a preparation method and application thereof. The microorganism and enzyme mixed preparation comprises citric acid, starch, carboxymethyl chitosan, bacillus subtilis, cellulase, cysteine and diatomite. The preparation method of the stripping agent comprises the following steps of: 1, sequentially adding the substances according to the proportion; 2, uniformly stirring for 15 minutes by using a V-shaped stirrer or a three-V stirrer; and 3, mixing the obtained mixture with pond water according to a mass ratio of 1: 100 to 1: 200, and uniformly stirring to obtain the microorganism and enzyme mixed preparation for stripping dirt of aquatic plants. Dirt on the surfaces of aquatic plants is rapidly removed, and photosynthesis is improved. A high-quality ecological environment rich in aquatic plants, which is clear in water, high in dissolved oxygen content in water and few in harmful substances in the water, is provided for cultured objects.

The invention relates to an extraction and preparation method for high-antioxidant dietary fiber. The extraction and preparation method comprises the following steps: (1) carrying out high-temperature passivation: maintaining waxberry dregs which are dried and heated at 100 DEG C for 10 minutes so as to passivate polyphenol oxidase; (2) carrying out ethylene softening: introducing ethylene at the fifth minute, and maintaining the concentration at 800-1000ppm, so that the fiber texture is softened; (3) carrying out enzymolysis: adding warm water in a ratio of material to liquor of 3:1, and cooling the mixture of the waxberry dregs and water to 60-80 DEG C, adding 400-450mg/L cellulase while stirring to react for 30 minutes; (4) carrying out compound enzymatic hydrolysis: adding a compound enzyme which is 0.1-0.5% of dry powder of the waxberry dregs by mass for enzymolysis, wherein the compound enzyme consists of amylase and papain in a ratio of 3:1, the pH value during enzymolysis is controlled to 6-8, the enzymolysis temperature is controlled at 60-80 DEG C, and the enzymolysis time is 60-90 minutes; and (5) carrying out filtering and drying: filtering the mixture after enzymolysis and drying the filter residue to obtain waxberry dietary fiber powder. The extraction and preparation method provided by the invention has the advantages that the extraction and preparation method is simple in process flow and short in production time; polyphenol substances can be fully stored; the product is light red; and the extraction and preparation process is free of environmental pollution.

The invention discloses a potato peel and radix ophiopogonis composite health beverage and a preparation method thereof, and belongs to the technical field of functional beverages. The beverage is composed of the following components: potato peel, radix ophiopogonis, hawthorn fruit, ligustrum lucidum, radix angelicae pubescentis, pulsatilla, ginger, pueraria lobata, astragalus, poria cocos, mangnolia officinalis, wide jujube seed, polygonum multiflorum, licorice, citric acid, fresh bamboo leaves, cellulase, amylase and white sugar. The preparation method comprises the following steps of determining the ratio, preparing a potato peel liquid, preparing a radix ophiopogonis juice, preparaing an auxiliary liquid, homogenizing, degassing, filling and sterilizing. The pototal peel obtained afterdeep processing of potatoes can be adopted for secondary utilization, and the radix ophiopogonis is added as a main material. The beverage has the advantages of immune regulation, blood sugar lowering, fatigue resistance and aging resistance.