Cellulase separation and purification method

[0016] Embodiment 1: Utilize Trichoderma reesei to ferment and produce cellulase, 500L fermentation tank liquid volume 400L ferments 6 days and finish; Measure the FPA enzymatic activity of wherein cellulase is 46.2U / ml, measuring method is People's Republic of China light industry Industry standard QB 2583-2003; plate and frame filter to remove large particles and mycelia in the fermentation broth to obtain 380L of fermentation broth, filtrate FPA enzyme activity 45.8U / ml; plate and frame filtrate is pumped into a low-temperature storage tank and cooled with 4°C cooling water It is circulated cold and stored below 15°C; the plate and frame filtrate is concentrated to 800U / ml through a 30KD ultrafiltration membrane, which takes 3 days, and the volume of the concentrated solution is 19.5L; 39g (0.2%, w / v) lactose is added to the concentrated solution Mix with 97.5g (0.5%, w / v) of silicon dioxide, stir evenly, and the mixed material passes through the PALL sterilization eq

[0018] Embodiment 2: Utilize Trichoderma reesei to ferment and produce cellulase, 500L fermenting tank liquid capacity 400L ferment 6 days and finish; Measure wherein the FPA enzyme activity of cellulase is 46.2U / ml, measuring method is People's Republic of China light industry Industry standard QB 2583-2003; plate and frame filter to remove large particles and mycelia in the fermentation broth to obtain 380L of fermentation broth, filtrate FPA enzyme activity 45.8U / ml; plate and frame filtrate is pumped into a low-temperature storage tank and cooled with 4°C cooling water It is circulated cold and stored below 15°C; the plate and frame filtrate is concentrated to 700U / ml through a 30KD ultrafiltration membrane, which takes 3 days, and the volume of the concentrated solution is 19.5L; 39g (0.2%, w / v) lactose is added to the concentrated solution Mix with 97.5g (0.5%, w / v) of silicon dioxide, stir evenly, and the mixed material passes through the PALL sterilization equipm

[0019] Embodiment 3: Utilize Trichoderma reesei to ferment and produce cellulase, 500L fermenting tank liquid capacity 400L ferment 6 days and finish; Measure wherein the FPA enzyme activity of cellulase is 46.2U / ml, measuring method is People's Republic of China light industry Industry standard QB 2583-2003; plate and frame filter to remove large particles and mycelia in the fermentation broth to obtain 380L of fermentation broth, filtrate FPA enzyme activity 45.8U / ml; plate and frame filtrate is pumped into a low-temperature storage tank and cooled with 4°C cooling water It is circulated cold and stored below 15°C; the plate and frame filtrate is concentrated to 600U / ml through a 30KD ultrafiltration membrane, which takes 3 days, and the volume of the concentrated solution is 19.5L; 39g (0.2%, w / v) lactose is added to the concentrated solution Mix with 97.5g (0.5%, w / v) of silicon dioxide, stir evenly, and the mixed material passes through the PALL sterilization equipm