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43 results about "Ultrafiltration" patented technology

Ultrafiltration (UF) is a variety of membrane filtration in which forces like pressure or concentration gradients lead to a separation through a semipermeable membrane. Suspended solids and solutes of high molecular weight are retained in the so-called retentate, while water and low molecular weight solutes pass through the membrane in the permeate (filtrate). This separation process is used in industry and research for purifying and concentrating macromolecular (10³ - 10⁶ Da) solutions, especially protein solutions.

System for preparing potassium sulfate from high-salt-content industrial wastewater and technology of system

ActiveCN105645439ATreatment involving filtrationWater/sewage treatment by ion-exchangeUltrafiltrationMirabilite
The invention relates to a system for preparing potassium sulfate from high-salt-content industrial wastewater and belongs to the field of water treatment. The high-salt-content industrial wastewater is pretreated through a regulating pool, a pipeline mixer, a high-density precipitate pool, a V-type filter pool, ion exchange resin and an ultrafiltration membrane system and then concentrated through a first section reverse osmosis membrane system and a second section reverse osmosis membrane system and then enters a nanofiltration membrane system for salt separation, nanofiltration produced water and thick water enter a plate-pipe type high-pressure flat sheet membrane system II and a plate-pipe type high-pressure flat sheet membrane system I respectively to be concentrated again, the concentrated thick water enters a freezing crystallization system for crystallization, crystals enter a potassium sulfate preparing system to be converted, freezing mother liquor and the concentrated nanofiltration produced water enter an advanced oxidizing system for oxidation and then enter an evaporative crystallization system for crystallization, and produced sodium sulfate crystal salt enters a potassium sulfate preparing system for conversion. The system has the advantages that the scale of the nanofiltration system is reduced, and investment cost is reduced; salt separation is thorough, and operation cost is low; the purity of the crystallization salt is high; mirabilite and sodium sulfateare converted into potassium sulfate, and the economic value is improved.
Owner:INNER MONGOLIA JIUKE KANGRUI ENVIRONMENTAL TECH

Method for producing ethanol from crop straw

ActiveCN101186943AEasy to separateTake advantage ofBiofuelsMicroorganism based processesSolid componentFiltration
The invention provides a process for producing ethanol with raw materials of straws. The process comprises preliminary treatment to crops straws, solid-liquid separation, hydrolyzed solid components and fermentation with yeasts after hyperfiltration and filtration enrichment by RO combination. The invention can simultaneously conduct evaporating concentration to liquid components, and produce corresponding by-product of lignosulfonate by adding sulfonating agent. The ethanol producing process of the invention greatly decreases the cost of industrial production which ferments straws to produce ethanol, and efficiently utilizes all the ingredients of crops straws.
Owner:ANHUI BBCA FERMENTATION TECH ENG RES

Preparation method of amphoteric polyether sulphone separation membrane

InactiveCN103263863ASemi-permeable membranesUltrafiltrationCarboxylic group
The invention discloses a preparation method of an amphoteric polyether sulphone separation membrane. The preparation method specifically comprises the following steps of: 1, taking a polyether sulphone flat ultrafiltration membrane, and removing an additive contained in the polyether sulphone flat ultrafiltration membrane to obtain a clean polyether sulphone flat ultrafiltration membrane; 2, performing plasma modification on the polyether sulphone ultrafiltration membrane obtained in the step 1 by using a controllable low-temperature plasma discharge device to obtain a modified polyether sulphone ultrafiltration membrane; and 3, taking an acrylic monomer, and grafting the modified polyether sulphone ultrafiltration membrane with the acrylic monomer to obtain the amphoteric polyether sulphone separation membrane. The polyether sulphone separation membrane prepared by the preparation method is an amphoteric membrane of which the surface contains amine groups and carboxyl, and the surface hydrophilicity is enhanced, so that the pollution resistance of the membrane is improved; and while the retention rate is increased, the pure water flux is greatly improved, so that the filtration efficiency is improved.
Owner:XI'AN POLYTECHNIC UNIVERSITY

Method for treating waste emulsion

The invention provides a method for treating waste emulsion. The method comprises the following steps: performing oil slick removal treatment on the waste emulsion to be treated to remove oil slick therein; performing oil-water separation treatment on the waste emulsion without oil slick to remove disperse oil therein; performing electrochemical treatment on the waste emulsion after the oil-water separation treatment to obtain roughly treated waste emulsion in which the oil slick and disperse oil are removed and the emulsified oil and dissolved oil are flocculated; and performing static settlement and ultrafiltration treatment on the roughly treated waste emulsion to obtain clear water without oil slick, disperse oil, emulsified oil or dissolved oil, wherein the electrochemical treatment is performed by use of energized aluminum plate and a nano ceramic membrane, and the ultrafiltration treatment is performed by use of the power-off nano ceramic membrane. Through the treatment of the method provided by the invention, the water quality of the yielding water is good, and the yielding water can completely meet the limitation requirements on the discharge into a public sewage treatment system in the Integrated Discharge Standard of Water Pollutants DB11 / 307-2013 of Beijing.
Owner:BEIJING SINORICHEN ENVIRONMENTAL PROTECTION

Purification method of teicoplanin

ActiveCN102964430AReduce usageAvoid the problem of remainingPeptide preparation methodsPurification methodsFiltration
The invention provides a purification method of teicoplanin, and the purification method comprises the following steps: (1) adjusting teicoplanin fermentation liquid to be alkaline, then performing filtration; (2) adjusting the filtrate obtained in step (1) to be acidic, eluting the filtrate through macroporous adsorption resin by using water and ammonia water in order, collecting the ammonia water eluate; (3) filtering the eluate obtained in step (2), separating the filtrate through silica gel chromatography, collecting the eluate of ethanol aqueous solution with a volume ratio of 10-50%; (4) adjusting the eluate obtained in step (3) to be acidic, performing ultrafiltration by an ultrafilter membrane with a cutoff molecular weight of 5000-30000 Da, preferably 10000 Da, so as to obtain an ultrafiltrate; and (5) performing nanofiltration of the ultrafiltrate obtained in step (4) through a nanofiltration membrane with a cutoff molecular weight of 100-1000 Da, preferably 200 Da, and concentrating to obtain the teicoplanin. The purification method of the invention improves the production efficiency of teicoplanin to the maximum extent, and thus realizes industrial production of high-purity teicoplanin.
Owner:LIVZON GROUP FUZHOU FUXING PHARMACEUTICAL CO LTD

Cellulase separation and purification method

InactiveCN102876647ASolve the phenomenon of enzyme inactivationHigh yield of enzyme activityMicroorganism based processesEnzymesBiotechnologyUltrafiltration
The invention discloses a cellulase separation and purification method. According to the method, fungus liquid deep layer fermentation cellulase fermentation liquid is subjected to plate frame filtering for removing large particle substances and hyphae in the fermentation liquid, the cellulase plate frame filter liquid is stored into a filter liquid storage tank, in order to avoid the enzyme activity loss, the low-temperature state is maintained, the cellulase plate frame filter liquid is subjected to ultrafiltration membrane concentration and is mixed with protecting agents of lactose and silicon dioxide, the uniform stirring is carried out, the mixed lactose materials pass through bacterium removing membrane equipment for filtering out sundry bacteria, bacterium removing cellulase enzyme material liquid is subjected to the spraying drying process to obtain cellulase powdery finished products. The cellulase powdery finished products separated and purified according to the method provided by the invention solve the problem of great loss of cellulase caused by sundry bacteria generated in the subsequent extraction process of the cellulase fermentation liquid, in addition, after the adoption of the protecting agent spraying, the spraying enzyme activity yield is improved by 18 percent, the cellulase yield of the whole purification process is improved by 23 percent, and the production cost is reduced.
Owner:熊鹏

Composite quality control product of liver function, and preparation method and application thereof

The invention relates to a composite quality control product of a liver function, and a preparation method and application thereof, wherein the composite quality control product comprises alpha-L-fucosidase, adenosine deaminase and 5'-nucleotidase; and the preparation method comprises the following steps of: taking a fresh pork liver as a raw material, and obtaining the composite quality control product of the liver function simultaneously containing three kinds of enzymes through processes of grinding, homogenizing, crushing, centrifuging, salting-out, purifying, ultrafiltration, freeze-drying and the like. By optimizing preparation process parameters, enzyme inactivation in the preparation process is avoided; meanwhile, impurities in the finished product are reduced; therefore, the purity of the target enzyme is improved; furthermore, the freeze-dried powder of the composite quality control product with better stability is obtained through a freeze-drying process; furthermore, a protective agent of enzyme solution is optimized, so that the thermal stability and long-term stability of the freeze-dried powder of the composite quality control product are enhanced; and thus, the composite quality control product of the liver function, which is simple and rapid in preparation method, low in cost and high in enzyme activity, uniformity and stability, is obtained.
Owner:WUHAN LIFE ORIGIN BIOTECH LTD

Additive used for increasing clone number of hybridoma cells and preparation method of additive

ActiveCN105462934APromote growthGrow fastFused cellsCell culture active agentsUltrafiltrationHybridoma cell
The invention provides an additive used for increasing the clone number of hybridoma cells and a preparation method of the additive, and relates to an immunological research technology. The preparation method includes the following steps that 1, culture solution supernatant for eukaryotic cells capable of expressing IL-6 protein is acquired, wherein the cell culture solution supernatant contains expressed IL-6 protein; 2, the cell culture solution supernatant is subjected to ultrafiltration and filtration sterilization in sequence, and a cell culture solution supernatant concentrated solution is obtained; 3, sodium selenite is added into the cell culture solution supernatant concentrated solution, and the additive is obtained. As proved by experimental data, the clone number of the hybridoma cells can be increased by nearly 5 times after the additive is adopted. The invention further provides a hybridoma cell culture medium containing the additive.
Owner:TARCINE BIOMED INC

Municipal waste harmless recycling method with sewage cyclic utilization

InactiveCN109500049AHarmlessRealize resource processingGeneral water supply conservationSolid waste disposalRefuse-derived fuelChemical reaction
The invention relates to a municipal waste harmless recycling method with sewage cyclic utilization. Municipal waste is sorted into waste raw materials and preparation raw materials of waste derived fuel in manual sorting, magnetic sorting and mechanical sorting modes, then the preparation raw materials of the waste derived fuel obtained in the previous process are prepared into the waste derivedfuel, additionally, the raw materials are mixed with clay, limestone and iron powder and are crushed so as to be calcined in a rotary kiln to prepare raw materials for building materials, fly ash andsmoke which are generated by calcination of the rotary kiln are treated in dedusting and chemical reaction modes, and finally, denitrification, nitrification, ultrafiltration and reverse osmosis treatment are performed on sewage, so that the waste cleaning water is purified. The municipal waste is converted into the raw materials for the building materials, the municipal waste can be recycled, sothat the use rate of the municipal waste is greatly increased, and the sewage generated in a waste treatment process can be purified and reused, so that water resources are saved.
Owner:DONGGUAN UNIV OF TECH

Efficient preparation method of low-value fish immunomdodulating peptides

The invention discloses an efficient preparation method of low-value fish immunomdodulating peptides, relates to the development and utilization of low-value fish bioactive peptides and related anti-inflammatory drug products, and belongs to the technical field of low-value fish processing; the preparation method comprises the steps of preparing nemipterus virgatus myosin by a salt extraction method, fully expanding a high-spiral structural domain of myofibrillar protein by using a high-pressure homogenization method; fine-tuning a protein solution to pH 7.5, adding trypsin, performing enzymatic hydrolysis for 90 minutes, promoting an ultrafiltration membrane to separate a nemipterus virgatus protein enzymatic hydrolysate by using impressed current, and obtain a peptide fragment of 500 to3000 components; finally obtaining a powdery product by a freeze-drying method; the method can improve the degree of hydrolysis of the nemipterus virgatus myosin peptide, and the biological propertiesand yield of the immunomodulating peptides; the anti-inflammatory activity of the immunomodulatory peptides prepared by the method is better; the invention realizes the deep processing and comprehensive utilization of the low-value fish, and has important meaning for improving the added value of the low-value fish.
Owner:FUJIAN AGRI & FORESTRY UNIV

Preparation method for purple sweet potato anthocyanin facial mask

InactiveCN110664688ACosmetic preparationsToilet preparationsUltrafiltrationEnzymatic hydrolysis
The invention discloses a preparation method for a purple sweet potato anthocyanin facial mask. The method comprises the following steps: 1) pre-treating fresh purple sweet potatoes; 2) adding acidified ethanol into the obtained purple sweet potato pulp, adding cellulase, performing ultrasonic treatment, performing sieving and filtering, performing separation and purification by macroporous resin,performing gradient elution, and performing concentration and drying to obtain a purple sweet potato anthocyanin extract; 3) performing enzymatic hydrolysis on silk, performing inactivation of enzyme, performing classification by ultrafiltration, and performing concentration and drying to obtain a silk fibroin peptide with a weight-average molecular weight of 2-10k; and 4) compounding the purplesweet potato anthocyanin extract and the silk fibroin peptide according to a ratio, adding other auxiliary materials, and performing uniform mixing to obtain the purple sweet potato anthocyanin facialmask. According to the method, the purple sweet anthocyanin extract obtained by pre-treating the purple sweet potatoes and performing extraction is compounded with the silk fibroin peptide accordingto the specific weight ratio, other auxiliary materials with a specific content are also used to prepare the purple sweet potato anthocyanin facial mask, and the obtained purple sweet potato anthocyanin facial mask is environmentally friendly and safe, and has excellent anti-acne, whitening, spot-fading and anti-wrinkle effects.
Owner:SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI

Method for preventing whole-house separate purifying device film from being polluted

The invention relates to the technical field of water purification, and in particular relates to a method for preventing a whole-house separate purifying device film from being polluted. The method comprises the following steps of: 1) carrying out ultrafiltration membrane backwashing on a hollow fiber ultrafiltration membrane of ultrafiltration equipment through tap water, and then carrying out positive ultrafiltration membrane washing; and 2) carrying out positive spiral-wound membrane washing on a spiral-wound membrane of nanofiltration or reverse osmosis equipment through water. According to the method for preventing the whole-house separate purifying device film from being polluted, the hollow fiber is subjected to ultrafiltration backwashing, positive ultrafiltration washing and positive spiral-wound membrane (nanofiltration or reverse osmosis) washing by the raw water of tap water when in downtime in light of the polluting conditions of the hollow fiber ultrafiltration membrane and the spiral-wound membrane (nanofiltration membrane or reverse osmosis membrane) of a core component of a whole-house separate water supplying device, the serious pollution of the hollow fiber ultrafiltration membrane and the spiral-wound membrane (nanofiltration membrane or reverse osmosis membrane) can be effectively avoided, the stability of the system in running can be ensured, the service life of the reverse osmosis membrane is prolonged, and the operation cost of the system is reduced.
Owner:ZHEJIANG CREATION ENVIRONMENT TECH

Biological pollution resistant reverse osmosis composite membrane and preparation method thereof

ActiveCN112973473AGood rejectionImprove antibacterial propertiesMembranesGeneral water supply conservationUltrafiltrationReverse osmosis
The invention discloses a biological pollution resistant reverse osmosis composite membrane and a preparation method thereof. The preparation method comprises the following steps: contacting a porous ultrafiltration support layer with a tannic acid aqueous solution; contacting with a ferric salt aqueous solution; contacting with a silver nitrate solution; contacting with a polyamine aqueous solution; and contacting with a multi-acyl chloride solution, drying in air at room temperature, and soaking in pure water to obtain the biological pollution resistant reverse osmosis composite membrane. Test results show that the anti-biological pollution reverse osmosis composite membrane prepared by the invention has excellent retention rate and antibacterial property, and meanwhile, the retention rate is kept to be 97.4% or above while relatively high water flux is achieved. In addition, the sterilization capability on staphylococcus aureus and escherichia coli is very high. Meanwhile, the membrane has excellent anti-adhesion performance on bacteria, and microbial contamination on the surface of the membrane can be effectively prevented.
Owner:TIANJIN POLYTECHNIC UNIV

Double-water-channel water purification tap combination filter element

The invention provides a double-water-channel water purification tap combination filter element. After being installed to a purpose-made water tap, the filter element has double water channels, so the water without needs for filtration and water in demands for filtration independently exist in the two channels, thereby ensuring that the user can use the filter element at low cost. The filter element comprises a central tube, wherein the inner cavity of the central tube is a direct-flow water channel; a PP (polypropylene) filter bowl is sheathed on one end of the central tube; the rim of the PP filter bowl is arranged towards the other end of the central tube; the inner wall of the PP filter bowl is provided with a PP cotton prefilter piece; the inner wall of the rim of the PP filter bowl is connected with one end of an ultrafiltration membrane shell in a sealing mode; the upper part of the ultrafiltration membrane shell is provided with a sintered activated carbon rod; the sintered activated carbon rod is provided with a central inner hole; a connecting cover is arranged between the ultrafiltration membrane shell and the sintered activated carbon rod; ultrafiltration membrane yarn is arranged in the ultrafiltration membrane shell; and a water outlet of the inner cavity of the ultrafiltration membrane shell communicates with a water inlet of the connecting cover.
Owner:SUZHOU KAHO POLYMER TECH CO LTD

Method for purifying nitrilase

InactiveCN102363775AImprove purification efficiencyHigh purityLyasesChemical industryUltrafiltration
The invention relates to a method for purifying nitrilase and belongs to a preparation method for the nitrilase. By the method, the purified nitrilase product is prepared from commercial coarse nitrilase by the simple process of preprocessing, ultrafiltration and classification, and freeze drying. The method is simple; the conventional simple equipment is adopted; the method is easy to operate and control, and convenient to popularize and apply, and the production is safe; in the production process, the reaction condition is mild, energy consumption is low, material resources are utilized completely, emission is avoided, and environmental protection is facilitated; and the produced product has high purity, activity and stability and can meet the requirement on preparation of products with high additional value. The product prepared by the method can be widely applied to the industries such as medicines, chemical industry, materials, agriculture and the like.
Owner:CHONGQING UNIV

Preparation process of rabies vaccine without antibiotic addition

ActiveCN112451658AAvoid the risk of adverse reactionsReduce impuritySsRNA viruses negative-senseViral antigen ingredientsPerfusion CultureUltrafiltration
The invention provides a preparation process of a rabies vaccine without antibiotic addition in the whole process from a cytotoxic strain source. A Vero cell pre-master cell bank, a master cell bank,a working cell bank, a CTN-1V strain pre-master seed batch, a master seed batch and a working seed batch are prepared by using a culture medium without antibiotic addition, a bioreactor and a flaky carrier are applied, and through performing pre-culture and perfusion culture on Vero cells, inoculating a working seed batch of rabies viruses, performing washing and exchanging, performing perfusion culture to obtain a virus solution, performing ultrafiltration concentration, performing inactivating, performing purifying, preparing a semi-finished product, performing subpackaging and performing freeze-drying, the rabies virus vaccine is obtained. By optimizing the process of the virus adding course of bioreactor culture, impurity residues in the rabies vaccine are effectively reduced, the residual quantity of bovine serum components, Vero cell DNA and Vero cell host proteins is reduced, compared with other vaccine products on the market, the prepared vaccine product has superiority in clinical trial safety, and the safety of biological products is ensured to the greatest extent.
Owner:CHANGCHUN ZHUOYI BIOLOGICAL CO LTD

Resource utilization method of fructus momordicae industrial waste

The invention provides a resource utilization method of fructus momordicae industrial waste. The resource utilization method comprises the following steps of performing preprocessing, performing mixedenzymolysis with cellulases and papain, performing ultrafiltration, performing strong acidic cation exchange resin chromatography, and performing freeze drying to prepare fructus momordicae protein powder. According to the characteristic that waste liquid generated by production of fructus momordicae shows acidic, acidic cellulases and papain are directly used for treating waste liquid, the maneuverability is high, and the method is suitable for industrial production. Besides, the enzymolysis efficiency is improved, the purpose of turning waste into wealth is realized, the fructus momordicaeresources are comprehensively utilized, considerable economic benefits are created, and the problem of environmental pollution is also solved. Besides, the technology process of the method is low in cost, fructus momordicae protein is produced from the fructus momordicae waste liquid, and the method can be widely applied to trade of health-care products and pharmaceutical industry. Waste can be turned into wealth, and the waste liquid can be recycled, so that the added value of the fructus momordicae is increased.
Owner:广西壮族自治区科学技术情报研究所

Method for separating and purifying ganoderan based on field flow separation technology

ActiveCN114874349AHigh purityHigh activityChemical industryUltrafiltrationUltrafiltrationSeparation technology
The invention provides a method for separating and purifying ganoderan based on a field flow separation technology. According to the method, extracted ganoderma lucidum crude polysaccharide is firstly subjected to protein removal for four times by adopting a Sevag method, then passes through an AF4 pool channel, is subjected to ultrafiltration for a period of time by adopting ultrapure water as a carrier liquid and adopting a 10kDa renewable cellulose membrane at an ultrafiltration flow rate of 0.2 mL / min, and finally is subjected to asymmetric field flow separation, ultrapure water is adopted as a carrier liquid during asymmetric field flow separation, and ultra-pure water is adopted as an ultra-pure liquid during asymmetric field flow separation. And the initial cross flow velocity index is reduced to 0.05 mL / min. According to the method, the protein removal frequency is reduced in the ganoderma lucidum polysaccharide separation and purification process, the carrier liquid in the whole process is ultrapure water, the dialysis desalting step is not needed, the separation and purification efficiency is improved on the basis of protecting the activity of the ganoderma lucidum polysaccharide, and the high-purity ganoderma lucidum polysaccharide is obtained.
Owner:HEBEI UNIVERSITY
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