Method for high-efficiency extraction of pumpkin pigments

[0019] Put 30kg of cooked pumpkin into the extraction tank, add 6kg of ethanol solution with a temperature of 40°C and a content of 50%, extract the pumpkin for 3 hours, dissolve the pumpkin red pigment in the ethanol solution, and collect the extract in the precipitate In the tank; dissolve chitosan in 2% acetic acid solution at a concentration of 1%, add it to the precipitation tank, which is 8% of the volume of the extract in the precipitation tank, stir for 1.5h, and let it stand for 3h; to the initial enzymatic hydrolysis Add 0.3kg of cellulase and 0.3kg of alkaline protease to the slurry of pumpkin, react at pH=5-6, 35°C for 6 hours, boil to inactivate the enzyme, add an appropriate amount of water, and stir well; first use a 1um bag Large flocs are removed by coarse filtration of the type filtration device, and the operating pressure difference on both sides of the ultrafiltration membrane is controlled to be 0.6Mpa. The filtrate is subjected to ultrasonic countercurrent ex

[0021] Put 40kg of cooked pumpkin into the extraction tank, add 8kg of ethanol solution with a temperature of 50°C and a content of 60%, extract the pumpkin for 4 hours, dissolve the pumpkin red pigment in the ethanol solution, and collect the extract in the precipitate In the tank; dissolve chitosan in 3% acetic acid solution at a concentration of 1%, add it to the precipitation tank, which is 8% of the volume of the extract in the precipitation tank, stir for 2h, and let stand for 4h; Add 0.8kg of cellulase and 0.8kg of alkaline protease to the pumpkin slurry, react at pH=5-6, 45°C for 7 hours, boil to inactivate the enzyme, add an appropriate amount of water, and stir well; first use a 1um bag Remove the large flocs by coarse filtration of the filtration device, control the operating pressure difference on both sides of the ultrafiltration membrane to 0.8Mpa, conduct ultrasonic countercurrent extraction of the filtrate for 45min, and filter the filtrate and filter residue after