The invention relates to a composite
quality control product of a
liver function, and a preparation method and application thereof, wherein the composite
quality control product comprises alpha-L-
fucosidase,
adenosine deaminase and 5'-
nucleotidase; and the preparation method comprises the following steps of: taking a fresh
pork liver as a
raw material, and obtaining the composite
quality control product of the
liver function simultaneously containing three kinds of enzymes through processes of
grinding, homogenizing, crushing, centrifuging,
salting-out, purifying,
ultrafiltration, freeze-
drying and the like. By optimizing preparation process parameters,
enzyme inactivation in the preparation process is avoided; meanwhile, impurities in the finished product are reduced; therefore, the purity of the target
enzyme is improved; furthermore, the freeze-dried
powder of the composite quality control product with better stability is obtained through a freeze-
drying process; furthermore, a protective agent of
enzyme solution is optimized, so that the
thermal stability and long-term stability of the freeze-dried
powder of the composite quality control product are enhanced; and thus, the composite quality control product of the
liver function, which is simple and rapid in preparation method, low in cost and high in enzyme activity, uniformity and stability, is obtained.