44 results about "Bacteria" patented technology

The invention relates to a method for producing an amino acid tobacco-specific foliar fertilizer by using abandoned fur. The method comprises the following steps of: degreasing, drying and crushing various abandoned animal hair in slaughterhouses, hair in barber shops and various abandoned animal leather which are used as main raw materials; carrying out microbial fermentation, mainly Freund crack mould or other keratin decomposing bacteria fermentation; then decomposing and filtering to prepare a crude amino acid solution; and chelating the following components in parts by weight: 30-50 parts of crude amino acid solution, 15-18 parts of potassium sulfate, 15-25 parts of ammonium sulfate, 15-18 parts of potassium dihydrogen phosphate, 3-6 parts of humic acid potassium, 1.3-2 parts of trace elements, 0.3-0.5 part of surfactant, 0.5-1 part of chelating agent and 0.15-0.2 part of plant growth regulating agent. The tobacco-specific foliar fertilizer has the effects of promoting growth of tobacco roots, enhancing the absorption and utilization of tobacco on soil and the fertilizer, promoting lengthening, widening and thickening of tobacco, enhancing the ability of disease resistance, and improving the quality and yield of tobacco leaves.

The invention discloses a method for preparing an organic fertilizer by fermenting cow dung compost. According to the method, two times of composting fermentation are adopted and a composting promoter is added, so that the fermentation speed and the composting degree can be quickly increased; meanwhile, polyacrylamide, bentonite, cocoanut active charcoal and the like are added to reduce the moisture in cow dung and achieve the effect of deodorizing; sodium bicarbonate and triple superphosphate are added to generate carbon dioxide which can expand the cow dung, so that the permeability is increased; the early-stage fermentation temperature of the cow dung can be increased by the heat released from reaction, so that the activity of natural fermentation bacteria agents in the cow dung is improved; meanwhile, when the heat generated by the cow dung after a period time of composting is overhigh, the polyacrylamide absorbs the moisture in the cow dung to achieve the effect of reducing the temperature and to reduce turning times; after the fermentation is completed, the organic fertilizer prepared by drying and dewatering is high in fertilizer efficiency, durable and efficient, has the effects of retaining water, sterilizing and improving soil, and can promote the rapid growth of crops, thus improving the yield and the quality of the crops.

The invention discloses an identification method for the clubroot resistance of alpine radish at the seedling stage. The identification method is characterized by comprising the following steps: S1, sieving soil of cultivated land, adding with a fertilizer and plant ash, mixing sterile soil with an organic fertilizer and calcium superphosphate, in addition, adding with plant ash, and preparing culture soil; S2, taking a hole tray as a culture device, adding the culture soil obtained in S1 into the hole tray, and sowing the seeds with the variety to be identified in the hole tray; S3, grinding fungus roots containing plasmodiophoromycetes, filtering the grinded fungus roots by adopting gauze, preparing a pathogenic spore solution, carrying out counting by adopting a blood counting chamber, preparing the spore solution into inoculating bacteria liquid with the concentration being 1x10<7> spores/ml for later use; S4, adding the culture soil into the hole tray for later use; and S5, completely watering the culture soil in the hole tray, when the water settles and the soil is slightly dry and is suitable for sowing, carrying out dibble seeding and earthing at the central point of each hole, establishing a small shed, carrying out normal field management, and investigating the morbidity at the 7th week after inoculation. According to the identification method provided by the invention, through the root irrigation identification method, the clubroot resistance of relevant experimental population can be improved, and thus the method has great values for the disease-resisting breeding practice and theoretical research.

The invention relates to a method for extracting unsaturated fatty acids from eucalyptus leaves. An organic solvent is used for extraction. Unsaturated fatty acid crude extracts are obtained by low-temperature crystallization treatment after dissolution, and more than 7 percent of unsaturated fatty acid component is obtained by CO2 critical extraction. The extraction method is simple, convenient, low in cost, stable in process and high in extraction efficiency. The unsaturated fatty acids in the eucalyptus leaves cotain polyunsaturated fatty acids such as linoleic acids and linolenic acids, are natural extracts, have the effects of resisting bacteria, resisting oxidation, depressing blood fat, lowering cholesterol, improving memory, suppressing tumors and the like, and can be widely applied to the fields of feeds, cosmetics, foods, biochemical engineering, even medicine and the like.

The invention discloses a method for lowering the content of nitrogen metabolism hazard materials in soy sauce through compound bacteria, and belongs to the technical field of microorganisms. By meansof combined fermentation of staphylococcus xylosus and Tetragenococcus halophilus, by lowering accumulation of biogenic amine and ethyl carbamate, the nitrogen metabolism hazard materials in soy sauce are controlled. In the soy sauce fermentation process, when soy sauce is mixed with finished koji and saline water according to the ratio of 1:1, when staphylococcus xylosus and Tetragenococcus halophilus are inoculated at the same time for fermentation, the total biogenic amine content in the finished soy sauce can be lowered by 64.2%, the content of ethyl carbamate is lowered by 77.2%, and meanwhile the amino nitrogen is increased by 61.1%.

A millipore ceramic type microbe filter for treating the gas or liquid containing bacteria and viruses features that the milliporous calcium phosphate ceramic is used as the filtering medium and the antibiotic agent is combined with said filtering medium. Its advantage is high effect to remove and kill the bacteria and viruses.

The invention relates to a water purification system for aquiculture and relates to the technical field of water treatment and purification. According to the water purification system for aquiculture, a culture pond, a water pump, a filter device, a precision filter and an ultraviolet (UV) sterilization device form a circulation loop through a pipeline, wherein the filter device consists of a cylindrical settling tank of which the bottom has a shape of an elliptical head and a plurality of filter tanks, which are arranged adjacent to each other in turn, a water body flow direction is formed, and filter cotton is arranged between the settling tank and the filter tanks; the UV sterilization device comprises two annular pipelines and a plurality of circular tubes which are arranged between the two annular pipelines; two ends of the circular tubes are communicated with the annular pipelines through pipelines; UV sterilization lamp tubes and protective tubes are arranged in the circular tubes; the UV sterilization lamp tubes are arranged in the protective tubes; and two ends of the UV sterilization lamp tubes and the protective tubes are fixed through end covers. The water purification system for aquiculture has a wide application range, and large-particle substances, bacteria, excrement and other dirt in the water are effectively settled and filtered; and moreover, physical and chemical conditions for aquatic water are stable, and the efficiency for water treatment is high.

The invention belongs to the field of medicine and personal care, and particularly relates to propolis-aloe bacteriostatic gel and a preparation method thereof. The propolis-aloe bacteriostatic gel isprepared from the following raw materials in percentage by mass: 8-12% of golden cypress, 8-10% of fructus cnidii, 6-11% of stemona, 9-12% of fructus kochiae, 7-9% of divaricate saposhnikovia roots,4-8% of seaweeds, 3-6% of aloes, 0.5-0.8% of propolis, 1-1.4% of chlorhexidine acetate, 0.8-1.2% of carbomer 940, 0.08-0.11% of methylparaben, 0.09-0.12% of triethanolamine, 0.9-2.1% of ethanol and 36-40% of purified water. The propolis-aloe bacteriostatic gel is propolis-aloe gel prepared through the compound compatibility of the propolis and the aloes, through the synergistic effect of the propolis and the aloes, the effects of inhibiting the growth of bacteria, promoting wound healing, diminishing inflammation, realizing sterilization, realizing moistening and nourishing, replenishing water, removing acne, removing eczema and realizing spot-fading can be effectively achieved, and especially, the bacteriostatic effect on escherichia coli, staphylococcus aureus, hemolytic streptococcus and the like is achieved.

The invention discloses a light and fluffy ecological bacteriostatic down fiber, which is characterized by being prepared from the following raw materials by weight part: 30-35 of white duck down, 2-4 parts of pearl fiber, 4-7 parts of mercerized cotton fiber, 3.7-5.8 parts of chitin, 1.3-2.3 parts of nano-ferroferric oxide, 6-9 parts of tetrabutyl titanate, 3-5 parts of straw charcoal, 0.6-0.8 part of sodium humate, 175-185 parts of 1-butyl-3-methylimidazole chloride, and a proper amount of water. In the invention, chitin molecules and down fiber are combined permanently through chemical modification, and are not easy to fall off, the chitin adsorbed on the down fiber surface is combined with the saliva of microbial phospholipid and penetrates into cells to adsorb anion equipped cytoplasm and undergo flocculation, the normal physiological activities of cells are disturbed and bacteria are killed, and the antibacterial properties are thorough. The ecological bacteriostatic down fiber prepared by the method provided by the invention has the characteristics of magnetic physical therapy efficacy, light weight and good fluffy degree, and excellent heat retention, etc.

The invention discloses a vehicular air purifier, which comprises a body. The body comprises a casing, an air inlet, a filter membrane component, a dust box, a post-filter air passage, an air compressor, an air outlet, radiation holes and a base plate. The filter membrane component is connected with the air inlet which is arranged on the front side of the casing, the dust box is arranged right below the filter membrane component, the post-filter air passage is connected with the other side of the filter membrane component, the air compressor is connected to the other end of the post-filter air passage, an air supply passage is connected to an air supply port of the air compressor, the air outlet is arranged on the rear side of the casing, and the radiation holes are arranged on the left side, right side and rear side of the casing. The filter membrane component is a hollow fiber microporous membrane, so that dust, bacteria and microbes in the air can be effectively separated out to purify the air. Further, with the dust box, people can clean the vehicular air purifier conveniently.

The invention discloses a bamboo insect breeding feed. The feed comprises raw materials and auxiliary materials, wherein the raw materials comprise the components in percentage by mass: 10%-15% of bamboo sections, 20%-25% of bamboo shoot casings, 10%-20% of fresh bamboo shoot cores, and 10%-15% of corn flour; the auxiliary materials comprise the components in percentage by mass: 10%-15% of bone meal, 20%-25% of dried marine algae powder, 5%-7% of eggs, 10%-15% of yellow mealworm powder, 10%-15% of lactic acid bacteria and an appropriate amount of water. The bamboo insect breeding feed disclosed by the invention has the beneficial effects that the feed which is used as a daily feed of bamboo insects is good in palatability, can effectively promote appetite, increase the daily gain and strengthen the disease resistance; particularly, fresh bamboos are used as internal stuffing fillers, so that the habits and the characteristics that the bamboo insects gnaw the bamboos can be effectively improved, and the bamboo insects adapt to be fed with the feed step by step.

The invention belongs to the field of daily chemical products, particularly relates to bacteriostatic foot powder and a preparation method thereof, and provides bacteriostatic foot powder. The bacteriostatic foot powder comprises the following components in percentages by weight: alkannin 1-3%, cinnaldehydrum 2-4%, berberine 1-5%, patchouli alcohol 2-4%, menthol 1-2%, alkyl glycoside 2-6%, tea saponin 0.5-1.5%, dipotassium glycyrrhizinate 1-3%, a deodorant 2-10%, ethanol 5-25% and corn flour 36.5-82.5%. The bacteriostatic foot powder not only can effectively improve foot hyperhidrosis and prevent and treat foot odour, but also can inhibit bacteria, diminish inflammation and resist allergy, and effectively treats tinea pedis. Meanwhile, the provided bacteriostatic foot powder is gentle in property, and is safe and non-irritant.

The invention provides a simple preservation method for magnaporthe oryzae strain. The simple preservation method comprises the following steps: by taking sterile water as a preservation carrier, preserving magnaporthe oryzae, specifically pouring a solid culture medium into a culture dish, inoculating the magnaporthe oryzae strain in the center of the culture medium, enabling the magnaporthe oryzae strain to grow and multiply at 22 DEG C, further transferring a magnaporthe oryzae strain black into a triangular flask with sterile water, and standing at 22-25 DEG C, thereby achieving simple andrapid medium/long-term preservation on the magnaporthe oryzae strain. The simple preservation method provided by the invention is simple, convenient and rapid, the treated magnaporthe oryzae strain is long in preservation period and stable in bacterium property, and transplanting does not need to be implemented at regular time within a preservation cycle, so that the preservation cost and the space are saved, the operation is convenient and rapid, expected bacteria can be preserved at any time, and the magnaporthe oryzae strain can be relatively convenient to activate, so that the method hasgreat application values for study on biological properties of magnaporthe oryzae and anti-disease breeding of rice.

The invention discloses antioxidant polypeptide expression pichia pastoris engineering bacteria and application. According to the antioxidant polypeptide expression pichia pastoris engineering bacteria and application, the genetic engineering technology is used to build and obtain antioxidant polypeptide expression pichia pastoris engineering bacteria KM71H FZM2014 AOP, CCTCC NO: M 2014552 which can achieve high expression of antioxidant polypeptide VECYGPNRPQF. The prepared antioxidant polypeptide can efficiently remove free radicals and show potent biological activity in an animal experiment with weaned piglets serving as a model. The antioxidant polypeptide has the advantages of being feasible in preparation method, easy to operate, high in expression level, low in cost, high in biological activity and the like and can be widely used in industries, such as breeding, food, health products, aquatic products, infants and cosmetics.

A spiral radiator and spiral funnel is characterized by comprising a spiral pipe (1) and a cover (2), ports are formed in the two ends of the spiral pipe, the cover is provided with a hole, and the port in one end of the spiral pipe communicates with the hole in the cover; or covers are arranged on the ports in the two ends of the spiral pipe correspondingly, and the ports communicate with holes in the covers. The following problems of pollution of viruses, bacteria, dust and the like in air to liquid, low heat dissipation efficiency, slowness, time consumption, refrigerating equipment or lower-temperature liquid needing and mark damage due to wetting are solved, wherein the problems are caused when people cool liquid.

The invention discloses an insect-resistant fusion gene mCryAb-VIP3A for coding insecticidal protein and an expression vector and application thereof, and aims at solving the technical problem that the drug resistance trend of insects to an insecticidal preparation cannot be restrained. According to the invention, an insect-resistant gene and the insect-resistant fusion gene mCryAb-VIP3A are designed, the protein encoded by the insect-resistant fusion gene is also provided, and an expression vector constructed by the insect-resistant fusion gene and recombinant bacteria constructed by the expression vector are designed. The insect-resistant fusion gene is applied to preparation of insect-resistant plant cells. The protein encoded by the insect-resistant fusion gene is applied to preparation of an insect-resistant preparation. The insect-resistant fusion gene disclosed by the invention can be stably expressed in corn, so that the biotoxicity generated by singly transferring the originalVIP3A (a) gene into a plant is avoided, the gene silencing phenomenon existing in exogenous gene expression is overcome, more choices are provided for plant pest control, and a new method is providedfor restraining the drug resistance trend of insects to an insecticidal preparation.

The invention discloses an engineering bacterium of beta-keto thiolase and 3-ketoacid coenzyme A transferase and its application, specifically a special-purpose expression engineering bacterium of beta-keto thiolase and 3-ketoacid coenzyme A transferase and its use in the production of acetoacetate. The engineering bacterium is obtained by leading the expression vector including the beta-keto thiolase and 3-ketoacid coenzyme A transferase in the Escherichia coli. The present invention also provides a preparation method of acetoacetate, which includes: fermenting the engineering bacterium expressing the beta-keto thiolase and 3-ketoacid coenzyme A transferase to obtain the beta-keto thiolase and 3-ketoacid coenzyme A transferase, and then adding substrate glucose and succinate solution, fermenting to obtain the acetoacetate. The special-purpose expression engineering bacterium of beta-keto thiolase and 3-ketoacid coenzyme A transferase of the present invention will play an important role in the industrialization production and wide application prospects.

The invention relates to the field of medical checkup quality control, and concretely relates to a liquid composite protective agent for long-term culture preservation, and a preparation method and an application method thereof. The liquid composite protective agent for long-term culture preservation is prepared from the following components according to 100mL of solution agent: 15 to 20mL of ethylene glycol, 10 to 15mL of glycerinum, 0.5 to 1g of nutrient bouillon, 0.3 to 0.8g of trehalose, 0.1 to 0.5g of brain infusion powder, and the balance distilled water. The liquid composite protective agent for long-term culture preservation provided by the invention has good protection on various bacteria cultures, can ensure the activity of the cultures at the temperature being minus 20 DEG C and below after being mixed with the cultures, cannot affect the biochemical characters of microorganisms, has no need to be freeze-dried, redissolved and preserved in dark, and is convenient to use and operate in clinic bacterial detection. The cultures protected by the liquid composite protective agent provided by the invention can stably survive for more than 1 year at the temperature being minus 20 DEG C, and stably survive for more than 10 years at the temperature being minus 70 DEG C, so that great convenience is provided for the long-term preservation of special cultures.

The invention discloses a traditional Chinese medicine composition for treating cervical erosion. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-15 parts of fallopia japonica, 10-15 parts of cyrtomium fortunei, 5-10 parts of groundsel, 15-20 parts of sophora flavescens, 12-18 parts of Chinese pulsatilla root, 5-10 parts of frankincense, 8-14 parts of cuttlebone, 10-15 parts of aloe leaf, 6-12 parts of shaddock peel, 16-22 parts of gallnut, 5-10 parts of acacia catechu, 10-15 parts of plantain herb, 20-25 parts of mother chrysanthemum, 4-9 parts of fig leaf, 18-24 parts of lithospermum, 5-10 parts of calendula, 9-16 parts of rheum officinale, 12-18 parts of subprostrate sophora, 5-10 parts of nauclea officinalis leaf and 10-15 parts of houttuynia cordata. The traditional Chinese medicine composition has the effects of clearing away heat and drying dampness, inhibiting bacteria and killing bacteria, diminishing inflammation and stopping pain, promoting blood circulation to remove blood stasis and removing slough and promoting growth of tissue regeneration, is obvious in curative effect, does not have a toxic or side effect, does not have an adverse reaction and symptoms, is high in cure rate, difficult to reoccur, safe and reliable and is worthy of promotion and popularization.

The invention discloses a preparation method of modified biochar immobilized petroleum degrading bacterium microspheres, which comprises the following steps: taking reed biomass powder, carbonizing, and activating in CO2 atmosphere to obtain a modified biochar carrier; placing the modified biochar carrier and a petroleum degrading bacterium solution in an immobilized culture medium for mixed adsorption to obtain the modified biochar adsorbing the microbial inoculum; a chitosan solution is used as an embedding agent to embed the modified biochar adsorbing the microbial inoculum, and the modified biochar immobilized petroleum degrading bacterium microspheres are obtained. The reed biochar is modified in a CO2 activation mode, the specific surface area and the pore volume of the immobilized carrier are effectively increased, chitosan is adopted as an embedding material, the degradation efficiency of petroleum degrading bacteria on petroleum is effectively improved, waste reeds are recycled, the problem of constructed wetland waste disposal can be solved, and the method is suitable for industrial production. The resource utilization of wastes is realized.

The invention discloses a method and a device for synchronously and efficiently removing nitrogen and phosphorus from low C/N domestic sewage by using an internal carbon source through a single-stage sequencing batch reactor, and belongs to the field of domestic sewage treatment. After domestic sewage enters the integrated short-cut nitrification endogenous short-cut denitrification phosphorus removal coupled anaerobic ammonia oxidation SBR reactor, an organic carbon source in the domestic sewage is converted into an internal carbon source in bacteria in a front anaerobic section, and the phosphorus release process is also completed; in the low-oxygen aeration stage, the phosphorus-accumulating bacteria absorb a part of phosphate in the sewage, and the ammonia-oxidizing bacteria oxidize ammonia nitrogen in a part of raw water into nitrite; and finally, in the anoxic section, part of nitrite and part of residual ammonia nitrogen generated in the aerobic section are converted into nitrogen by anaerobic ammonia oxidizing bacteria, the generated nitrate nitrogen is reduced into nitrite by denitrifying phosphorus-accumulating bacteria and denitrifying glycogen-accumulating bacteria, and the denitrifying phosphorus-accumulating bacteria further absorb phosphate. According to the method, the carbon source in the raw water is fully utilized to realize synchronous nitrogen and phosphorus removal of the low C/N domestic sewage, the ineffective loss of the carbon source is reduced, the energy consumption is reduced, and the sludge yield is relatively low.